• 한국식품조리과학회지
• /
• 제13권2호
• /
• pp.87-91
• /
• 1997

방사구와 비방사구에서 채취한 달걀 난황을 배소·압착하여 얻은 난황유를 Folch법으로 정제한 후 gas chromatography로 지방산 조성을 분석한 결과는 다음과 같다. 난황유의 총지질과 중성지질의 지방산 조성은 oleic acid, palmitic acid, linoleic acid 순으로 주성분을 이루었고, 당지질은 palmitic acid, oleic acid, stearic acid, lauric acid 순으로, 인지질은 oleic acid, lauric acid, palmitic acid 순으로 주성분을 나타냈다. 포화지방산 함량은 방사구 보다 비방사구에서 약간 높았고, 포화지방산에 대한 불포화지방산의 비율(USFA/SFA)은 비방사구보다 방사구에서 높았다.

• 대한수의학회지
• /
• 제40권3호
• /
• pp.551-561
• /
• 2000

Swine respiratory diseases have induced severe economic losses in swine industry worldwide. Several methods have been developed and applied to prevent and control the disease. However, those are still problematic in swine industry. Recently, the use of egg yolk antibodies with several advantages was introduced and applied to control diseases in animal as well as human. As the first step of the use of egg yolk antibodies in the control of the swine respiratory diseases, we investigated the immunogens of the causative agensts of the diseases and immune response in egg yolk of hens immunized with them. Bacterial antigens prepared from Bordetella bronchiseptica, Pasteurella multocida 3A and 4D, and Actinobacillus pleuropneumaniae serotype 2 and 5 were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blot and toxicity test in mice. The antigens were injected into laying hens in order to produce antibodies against them in egg yolk. After chickens were immunized three times in 2 weeks interval, the profile of antibody production was examined by ELISA. The production of antibody in egg yolk was started in 2 weeks after the first injection, reached peak in 6-8 weeks and maintained until 12 weeks. Of two adjuvants used in this study, ISA70 was more effective than aluminum hydroxide gel in enhancing immunogenecity, laying rates and safety in hens. These results suggested that egg yolk antibodies could be a good source for production of antibodies specific to pathogenic bacteria inducing respiratory diseases of swine.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권11호
• /
• pp.1503-1509
• /
• 2010

Forty-eight 40-wk-old Hi-sex laying hens were individually caged in an environmentally controlled house to evaluate the effect of garlic (Allium Sativum) juice administration on egg production, egg quality, and yolk cholesterol. Garlic juice was prepared by blending pealed garlic cloves with distilled water (1:1, w/w). Hens were randomly divided into four equal groups; one served as a control and the other three groups were individually gavaged, 3.75 ml, 7.5 ml, or 15 ml garlic juice, three times a week, which respectively represented 0.25, 0.50 and 1% of body weight. Egg production was recorded on a daily basis; egg weight, albumen height, albumen and yolk pH, Haugh unit, and bacterial count of E. coli-challenged eggs were recorded at day of oviposition (day-1) and after 5 and 10 days of storage at room temperature. Yolk cholesterol content was analyzed for five successive weeks. Garlic juice increased (p<0.05) egg weight and mass with no change in egg production intensity. Garlic juice administration recorded higher (p<0.05) albumen height and improvement in Haugh unit. Also, eggs from garlic-treated hens recorded lower (p<0.05) albumen and yolk pH when compared to eggs collected from control hens. Garlic reduced (p<0.05) the $log_{10}$ of bacterial count in egg contents linearly when challenged with E. coli. Egg-yolk cholesterol content was not influenced by garlic juice administration. It is concluded that garlic juice improved performance characteristics and may increase egg shelf life as indicated by egg quality improvement and lower bacterial count of E. coli-challenged eggs. The levels of garlic juice used in this study were insufficient to influence egg yolk cholesterol.

• 한국가금학회지
• /
• 제16권4호
• /
• pp.233-238
• /
• 1989

난황과 난백을 냉동저장 할 경우 발생하는 이화학적 변화를 조사함으로써 계란의 효율적인 저장기법을 확립하고자 실시 한 본 실험의 결과를 요약하면 다음과 같다. 1. 난황액을 냉동저장할 경우 Gel화가 발생하였으며 난백액의 경우 포립성과 포립안전성이 감소하였다. 2. 난자액의 동결저장중 Gel화 방지를 위한 각종 동결방지제(소금, 포도당, 설탕, 각종 amide류) 종에서 소금이 가장 효과적이었다. 3. 난황액과 난백액을 $-5^{\circ}C$와 $-20^{\circ}C$에서 냉동저장할 경우 점도가 증가하였으며 색택의 변화가 일어났는데 난백액의 색택변화는 미약하였다. 4. 난황액에 5% NaCl(소금)을 첨가하여 $-5^{\circ}C$에서 저장하는 방법이 난황의 Gel화를 방지하고 경제적으로 저장할 수 있는 방법이었다. 5. 소금을 첨가하여 냉동저장할 경우 난황액의 유화특성은 다소 감소하였다.

• 한국가금학회지
• /
• 제33권3호
• /
• pp.211-215
• /
• 2006

Genistein의 전이율을 조사하기 위하여 순도 97% 이상의 genistein 250 mg을 펠렛으로 성형한 후 500 mg및 1 g의 용량으로 각각 2, 4개씩 산란계의 어깨 부위 피하에 이식하여 계란의 난황 내에서 검출되는 genistein의 함량을 HPLC 및 HPLC-MS를 이용하여 분석하였다. Genistein 500 mg 처리구에서, 12, 18, 30일에 각각 218, 395 ng/g, 500 ng/g의 genistein 이 전이되어 59일까지 계속적으로 유지되었다. 또한 genistein 1,000 mg 처리구에서는 12일 후에는 240 ng/g, 18일 후에는 406.5 ng/g, 30일 후에는 514 ng/g의 genistein이 전이되어 59일까지 계속적으로 유지되었다. 본 연구를 통하여, 500 ng/g 이상의 genistein이 함유된 계란을 59일 이상 지속적으로 생산할 수 있어 피하이식을 통한 비급이 방식은 기존의 급이 방식에 비해 20배 이상 효과적이었다.

• 한국가금학회지
• /
• 제35권3호
• /
• pp.219-224
• /
• 2008

63주령 ISA Brown 산란계 총 96수를 사용하여, 12일 동안 난황에서의 biological astaxanthin과 chemical astaxanthin 및 capxanthin의 축적을 통한 착색 효과를 비교하였다. 대조구 사료에는 착색제를 첨가하지 않았고, 효모 Phaffia rhodozyma 3%를 대조구 사료에 첨가하여 astaxanthin 함량이 22.5 mg/kg 되게 한 후, 화학적으로 합성한 astaxanthin을 대조구 사료에 45 mg/kg 첨가한 후 및 paprika로부터 추출한 capxanthin을 대조구 사료에 45 mg/kg 첨가구 모두 4개의 처리구들을 두었다. 처리당 6반복, 반복당 4수씩을 임의로 배치하였다. Astaxanthin은 생물학적 및 화학적 급원 모두 급여 9일째까지 축적이 이루어졌다. Capxanthin의 경우, 난황에서 축적되지는 않았고 이로부터 파생되어진 물질들이 급여 $6{\sim}9$일 후에 미미하게 검출되었다. 난황에서의 astaxanthin 축적 정도는 급여한 농도에 비례하였다. 난황의 색깔을 제외하면, 달걀의 다른 품질 요인들은 처리구들 간의 차이가 거의 없었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제24권10호
• /
• pp.1447-1455
• /
• 2011

Sixty four, 30-week-old, Lohmann Brown-Lite laying hens were randomly allocated to one of four treatments with eight replicates per treatment and two hens per replicate for a 10 week study. The control diet was a high energy (11.81 MJ/kg) diet and the moderate energy (11.39 MJ/kg) diets were formulated dropping the level of animal fat. The three moderate energy diets were fed either unsupplemented (0.0%) or supplemented with 0.05 or 0.10% glycine. There were no significant differences (p>0.05) in hen-day egg production, egg weight, feed intake or feed conversion between birds fed the unsupplemented moderate energy and high energy diets. Significant differences were detected concerning egg components and quality measurements as assessed by albumen percentage (p = 0.02), yolk weight (p = 0.02), yolk percentage (p<0.01), yolk to albumen ratio (p<0.01) and yolk color (p = 0.01) between birds fed the unsupplemented moderate and high energy diets. Glycine supplementation of the moderate energy diet linearly increased (p<0.01) egg weight and feed intake with no significant (p>0.05) effects on egg production or feed conversion. Glycine supplementation significantly increased egg content (p<0.01), albumen weight (p<0.01) and percentage (p<0.01) as well as yolk weight (p<0.01) while yolk percentage (p = 0.04), yolk to albumen ratio (p = 0.01) and egg shell percentage (p<0.01) were linearly decreased. Supplementation with glycine produced a tendency (p = 0.09) towards an increase in the percentage of large eggs (63-72.9 g) produced with a concomitant decrease in the percentage of small (below 53 g) eggs (p = 0.09). The overall results of this study indicate that glycine supplementation of laying hen rations has the potential to increase egg production and weight. These increases appeared to be mediated through increases in feed intake and the ileal digestibility of fat and energy.

• 한국축산식품학회지
• /
• 제32권5호
• /
• pp.561-570
• /
• 2012

The objective of this study was to evaluate the effects of an atmospheric pressure plasma (APP) jet on L. monocytogenes inactivation, quality characteristics, and genotoxicological safety of cooked egg white and yolk. APP treatment using He gas resulted in a 5 decimal reduction in the number of L. monocytogenes in cooked egg white, whereas that using $He+O_2$, $N_2$, and $N_2+O_2$ decreased the number further, and to undetectable levels. All treatments of cooked egg yolk resulted in undetectable levels of inoculated L. monocytogenes. There were no viable cells of total aerobic bacteria after APP treatment on day 0 while the control showed approximately 3-4 Log CFU/g. On day 7, the numbers of total aerobic bacteria had increased by approximately 3 log cycles in cooked egg white, but there were no viable cells in cooked egg yolk after 2 min of APP jet. APP treatment decreased the $L^*$-values of cooked egg white and yolk significantly on day 0. No significant sensory differences were found among the cooked egg white samples, whereas significant reductions in flavor, taste, and overall acceptability were found in cooked egg yolks treated with APP jets. SOS chromotest did not reveal the presence of genotoxic products following APP treatments of cooked egg white and yolk. Therefore, it can be concluded that APP jets can be used as a non-thermal means to enhance the safety and extend the shelf-life of cooked egg white and yolk.

• 한국가축번식학회지
• /
• 제4권1호
• /
• pp.28-34
• /
• 1980

This experiment was carried out to study effects fo dosage and insemination interval on fertility and hatchability of whole semen and diluted semen with yolk skim milk and skim milk dilutors. The results obtained are as follows: 1. Whole semen showed higher fertility than diluted semen with yolk skim milk and skim milk dilutors. In case of diluted semen, the fertility was higher in 0.04$m\ell$ dosage than 0.02$m\ell$ and in skim milk than yolk skim milk dilutor. 2. The average fertility in inseminational intervals of 6, 5, 4 and 3 days was 52.4, 35.5, 48.7 and 44.2% in whole semen and 40.6, 17.2, 13.9 and 20.5% in 0.04$m\ell$ diluted semen with yolk skim milk dilutor. The fertility was not improved by shortening of insemination interval. 3. There was no considerable difference in hatchability of fertilized egg among the dosage of 0.02$m\ell$ of whole semen, 0.02$m\ell$ and 0.04$m\ell$ of diluted semen with yolk skim milk, and among the insemination intervals of 6, 5, 4 and 3 days. 4. Some differences in fertility among the passed days after insemination were decreased in the whole semen by shortening insemination interval from 6 and 5 days to 4 and 3days and also decreased in the diluted semen by shortening it to 3 days and by increasing dosage from 0.02$m\ell$ to 0.04$m\ell$. 5. Hatchability of fertilized egg showed no difference among the passed days during 6 days insemination interval both in the whole semen and the diluted semen. 6. The whole semen and the diluted semen with skim milk had not considerable difference in fertility among the passed days during 3 and 4 days insemination intervals, but the diluted semen with yolk skim milk had. 7. Hatchability of fertilized egg from the whole semen diluted semen with yolk skim milk and skim milk dilutors showed no difference among the passed days during 3 and 4 days insemination intervals.

• 센서학회지
• /
• 제24권3호
• /
• pp.151-154
• /
• 2015

The gas sensing characteristic of $MoS_2-MoO_2$ composite yolk-shell spheres were investigated. $MoO_3$-carbon composite spheres were prepared by ultrasonic spray pyrolysis of aqueous droplets containing Mo-source and sucrose in nitrogen, which were converted into $MoO_3$ yolk-shell spheres by heat treatment at $400^{\circ}C$ in air. Subsequently, $MoS_2-MoO_2$ composite yolk-shell spheres were prepared by the partial sulfidation of $MoO_3$. The $MoS_2-MoO_2$ composite yolk-shell spheres showed relatively low and irreversible gas sensing characteristics at < $200^{\circ}C$. In contrast, the sensor showed high and reversible response (S=resistance ratio) to 5 ppm $NO_2$ (S=14.8) at $250^{\circ}C$ with low cross-responses (S=1.17-2.13) to other interference gases such as ethanol, CO, xylene, toluene, trimethylamine, $NH_3$, $H_2$, and HCHO. The $MoS_2-MoO_2$ composite yolk-shell spheres can be used as reliable sensors to detect $NO_2$ in a selective manner.