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http://dx.doi.org/10.5851/kosfa.2012.32.5.561

Effect of Atmospheric Pressure Plasma Jet on Inactivation of Listeria monocytogenes, Quality, and Genotoxicity of Cooked Egg White and Yolk  

Lee, Hyun-Jung (Department of Animal Science and Biotechnology, Chungnam National University)
Song, Hyun-Pa (Department of Radiation Biotechnology, Advanced Radiation Technology Institute)
Jung, Hee-Soo (Agency for Defense Development)
Choe, Won-Ho (Department of Physics, Korea Advanced Institute of Science and Technology)
Ham, Jun-Sang (Quality Control and Utilization Division, National Institute of Animal Science, RDA)
Lee, Jun-Heon (Department of Animal Science and Biotechnology, Chungnam National University)
Jo, Cheo-Run (Department of Animal Science and Biotechnology, Chungnam National University)
Publication Information
Food Science of Animal Resources / v.32, no.5, 2012 , pp. 561-570 More about this Journal
Abstract
The objective of this study was to evaluate the effects of an atmospheric pressure plasma (APP) jet on L. monocytogenes inactivation, quality characteristics, and genotoxicological safety of cooked egg white and yolk. APP treatment using He gas resulted in a 5 decimal reduction in the number of L. monocytogenes in cooked egg white, whereas that using $He+O_2$, $N_2$, and $N_2+O_2$ decreased the number further, and to undetectable levels. All treatments of cooked egg yolk resulted in undetectable levels of inoculated L. monocytogenes. There were no viable cells of total aerobic bacteria after APP treatment on day 0 while the control showed approximately 3-4 Log CFU/g. On day 7, the numbers of total aerobic bacteria had increased by approximately 3 log cycles in cooked egg white, but there were no viable cells in cooked egg yolk after 2 min of APP jet. APP treatment decreased the $L^*$-values of cooked egg white and yolk significantly on day 0. No significant sensory differences were found among the cooked egg white samples, whereas significant reductions in flavor, taste, and overall acceptability were found in cooked egg yolks treated with APP jets. SOS chromotest did not reveal the presence of genotoxic products following APP treatments of cooked egg white and yolk. Therefore, it can be concluded that APP jets can be used as a non-thermal means to enhance the safety and extend the shelf-life of cooked egg white and yolk.
Keywords
atmospheric pressure plasma jet; cooked egg; L. monocytogenes; quality; genotoxicity;
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1 Basaran, P., Basaran-Akgul, N., and Oksuz, L. (2008) Elimination of Aspergillus parasiticus from nut surface with low pressure cold plasma treatment. Food Microbiol. 25, 626-632.   DOI   ScienceOn
2 Bogaerts, A., Neyts, E., Gijbels, R., and Mullen, J. (2002) Gas discharge plasmas and their applications. Spectrochim. Acta Part B. 57, 609-658.   DOI   ScienceOn
3 Deng, S., Ruan, R., Mok, K. C., Huang, G., Lin, X., and Chen, P. (2007) Inactivation of Escherichia coli on almonds using nonthermal plasma. J. Food Sci. 72, M62-M66.   DOI   ScienceOn
4 Devlieghere, F., Vermeiren, L., and Debevere, J. (2004) New preservation technologies: Possibilities and limitations. Int. Dairy J. 14, 273-285.   DOI   ScienceOn
5 Egan, H., Kirk, R. S., and Sawyer, R. (1981) Pearson's chemical analysis of foods. UK: Essex: Longman scientific and technical.
6 European Food Safety Authority. (2011) http://www.efsa.europa.eu/en/topics/topic/foodbornediseases.htm. Last access date was May, 16th, 2011.
7 Gweon, B., Kim, D. B., Moon, S. Y., and Choe, W. (2009) Escherichia coli deactivation study controlling the atmospheric pressure plasma discharge conditions. Curr. Appl. Phys. 9, 625-628.   DOI   ScienceOn
8 Jung H., Gweon, B., Kim, D. B., and Choe, W. (2011) A simple approach to surface modification using polytertrafluoroehylene (PTFE) with laminar and turbulent flows of micro plasma jet at atmospheric pressure. Plasma Process Polym. 8, 533-541.
9 Kim, B., Yun, H., Jung, S., Jung, Y., Jung, H., Choe, W., and Jo C. (2011) Effect of atmospheric pressure plasma on inactivation of pathogens inoculated onto bacon using two different gas compositions. Food Microbiol. 28, 9-13.   DOI   ScienceOn
10 Kim, H. J., Ham, J. S., Kim, K., Ha, J. H., Ha, S. D., and Jo, C. (2010) Quality evaluation of sliced and pizza cheeses treated by gamma and electron beam irradiation. Asian-Aust. J. Anim. Sci. 23, 1112-1117.   과학기술학회마을   DOI   ScienceOn
11 Korachi, M., Gurol, C., and Aslan, N. (2010) Atmospheric plasma discharge sterilization effects on whole cell fatty acid profiles of Escherchia coli and Staphylococcus aureus. J. Electrostat. 68, 508-512.   DOI   ScienceOn
12 Korean Food and Drug Administration. (2011) http://www.kfda.go.kr/fm/index.do?nMenuCode=67. Last access date was March, 3rd, 2011.
13 Kruk, Z. A., Yun, H., Rutley, D. L., Lee, E. J., Kim, Y. J., and Jo, C. (2011) The effect of high pressure on microbial population, meat quality and sensory characteristics of chicken breast fillet. Food Control 22, 6-12.   DOI   ScienceOn
14 Lee, H. J., Jung, H., Choe, W., Ham, J. S., Lee, J. H., and Jo, C. (2011) Inactivation of Listeria monocytogenes on agar and processed meat surfaces by atmospheric pressure plasma jets. Food Microbiol. 28, 1468-1471.   DOI   ScienceOn
15 Lee, K., Paek, K. H., Ju, W. T., and Lee, Y. (2006) Sterilization of bacteria, yeast, and bacterial endospores by atmospheric- pressure cold plasma using helium and oxygen. J. Microbiol. 44, 269-275.
16 Marzin, D.. R., Olivier, P., and Vophi, H. (1986) Kinetic determination of enzymatic activity and modification of the metabolic activation system in the SOS Chromotest. Mutat. Res. 164, 356-359.
17 Mersch,V., Mersch-Sundermann, V., Kevekordes, S., and Mochayedi, S. (1991) Sources of variability of the Escherichia coli PQ37 genotoxicity assay, Mutat. Res. 252, 51-60.   DOI   ScienceOn
18 Marsili, L., Espie, S., Anderson, J. G., and Macgregor, S. J. (2002) Plasma inactivation of food-related microorganisms in liquids. Radiat. Phy. Chem. 65, 507-513.   DOI   ScienceOn
19 Moisan, M., Barbeau, B., Moreau, S., Pelletier, J., Tabrizian, M., and Yahia, L. H. (2001) Low temperature sterilization using gas plasmas: a review of the experiments and an analysis of the inactivation mechanisms. Int. J. Pharm. 226, 1-21.   DOI   ScienceOn
20 Moisan, M., Barbeau J., Crevier, M. ED-C., Pelletier, J., Philip, N., and Saoudi, B. (2002) Plasma sterilization: methods and mechanisms. Pure Appl. Chem. 74, 349-358.   DOI   ScienceOn
21 Montengro, J., Ruan, R., Ma, H., and Chen, P. (2002) Inactivation of E. coli O157:H7 using a pulsed nonthermal plasma system. J. Food Sci. 67, 646-648.   DOI   ScienceOn
22 Moon, S. Y., Kim, D. B., Gweon, B., Choe, W., Song, H. P., and Jo, C. (2009) Feasibility study of the sterilization of pork and human skin surfaces by atmospheric pressure plasmas. Thin Solid Films 517, 4272-4275.   DOI   ScienceOn
23 Montie, T. C., Kelly-wintenberg, K. and Roth, and J. R. (2000) An overview of research using the one atmosphere uniform glow discharge plasma (OAUGDP) for sterilization of surfaces and marterials. IEEE Trans. Plasma Sci. 28,41- 50.   DOI   ScienceOn
24 Naveed, M. A., Qayyum, A., Ali, S., and Zakaullah, M. (2006) Effects of helium gas mixing on the production of active species in nitrogen plasma. Phys. Lett. A. 359, 499- 503.   DOI   ScienceOn
25 Niemira, B.A., and Sites, J. (2008) Cold plasma inactivates Salmonella Stanley and Escherichia coli O157:H7 inoculated on golden delicious apples. J. Food Prot. 71, 1357- 1365.
26 Quillardet, P., and Hofnung, M. (1985) The SOS Chromotest, a colorimetric bacterial assay for genotoxins procedures. Mutat. Res. 147, 65-78.   DOI   ScienceOn
27 Ragni, L., Berardinelli, A., Vannini, L., Montanari, C., Sirri, F., Guerzoni, M. E., and Guarnieri, A. (2010) Non-thermal atmospheric gas plasma device for surface decontamination of shell eggs. J. Food Eng. 100, 125-132.   DOI   ScienceOn
28 Song, H. P., Kim, B., Choe, J. H., Jung, S., Moon, S. Y., Choe, W., and Jo, C. (2009a) Evaluation of atmospheric pressure plasma to improve the safety of sliced cheese and ham inoculated by 3-strain cocktail Listeria monocytogenes. Food Microbiol. 26, 432-436.   DOI   ScienceOn
29 Song, H. P., Shin, E. H., Yun, H. J., Jo, C., and Kim, D. H. (2009b) Establishing the genotoxicological safety of gammairradiated egg white and yolk. Korean J. Food Preserv. 16, 782-788.
30 World Health Organization. (2007) Food safety and foodborne illness. http://www.who.int/mediacentre/factsheets/fs237/en/index.html. Last access date was Feb, 20th, 2011.
31 Yu, S. Q., Huang, C., Hsieh H.F., Huff, F., and Duan, Y. (2006) Sterilization effects of atmospheric cold plasma brush. Appl. Phys. Lett. 88, 1-3.
32 Yun, H., Kim, B., Jung, S., Kruk, Z. A., Kim, D. B., Choe, W., and Jo, C. (2010) Inactivation of Listeria monocytogenes inoculated on disposable plastic tray, aluminum foil, and paper cup by atmospheric pressure plasma. Food Control 21, 1182-1186.   DOI   ScienceOn