• Title/Summary/Keyword: Yield response

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Response Surface Methodological Approach for Optimization of Enzymatic Synthesis of Sorbitan Methacrylate

  • Jeong, Gwi-Taek;Lee, Kyoung-Min;Kim, Hae-Sung;Lee, Woo-Tai;Sunwoo, Chang-Shin;Park, Don-Hee
    • 한국생물공학회:학술대회논문집
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    • 2005.10a
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    • pp.511-516
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    • 2005
  • Sorbitan methacrylate was synthesized from sorbitan dehydrated from D-sorbitol using an immobilized lipase. To optimize the enzymatic synthesis of sorbitan methacrylate, response surface methodology was applied to determine the effects of five-level-four-factors and their reciprocal interactions on sorbitan methacrylate biosynthesis. A total of 30 individual experiments were performed, which were designed to study reaction temperature, reaction time, enzyme amount and substrate molar ratio. A statistical model predicted that the highest conversion yield of sorbitan methacrylate was 100%, at the following optimized reaction conditions: a reaction temperature of 43.06 $^{\circ}C$, a reaction time of 164.25 mins., an enzyme amount of 7.47%, and a substrate molar ratio of 3.98:1. Using these optimal factor values under experimental conditions in four independent replicates, the average conversion yield reached 98.7%${\pm}$1.2% and was well within the value predicted by the model.

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Response Surface Methodological Approach for Optimization of Removal of Free Fatty Acid in Crude Oil

  • Jeong, Gwi-Taek;Lee, Kyoung-Min;Yang, Hee-Seung;Park, Seok-Hwan;Kim, Jae-Hoon;Kim, Do-Man;Park, Don-Hee
    • 한국생물공학회:학술대회논문집
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    • 2005.10a
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    • pp.904-909
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    • 2005
  • To optimize the removal of free fatty acid in crude vegetable oil, response surface methodology was applied to determine the effects of five level-four factors and their reciprocal interactions on removal of free fatty acid. A total of 30 individual experiments were performed, which were designed to study reaction temperature, reaction time, catalyst amount and methanol amount. A statistical model predicted that the highest removal yield of free fatty acid was 99.8%, at the following optimized reaction conditions: a reaction temperature of 64.99$^{\circ}C$, a reaction time of 36.20 mins., an catalyst amount of 13.01% (w/v), and a methanol amount of 15% (v/v). Using these optimal factor values under experimental conditions in three independent replicates, the average removal yield was well within the value predicted by the model.

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Competition for Water in Two Populations of Impatiens pallida (Balsaminaceae) from Contrasting Water Environments (수분환경이 다른 서식지에서 자란 Impatiens pallida 의 두 개체군간 수분에 대한 경쟁)

  • Yang, Hyo-Sik;James B. McGraw
    • The Korean Journal of Ecology
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    • v.19 no.2
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    • pp.165-178
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    • 1996
  • We investigated the role of competition in adaptation to varying water availability levels for two ecotypically-differentiated populations of Impatiens pallida found naturally in low- vs. high-water environments. In a greenhouse experiment, seedlings were grown in pure cultures at two densities (n=1 and 2 plants per pot) and in mixed cultures (n=2) under low-, medium- and high-water treatments. The two populations were shown to be genetically distinct across the range of environmental conditions in the greenhouse experiment, confirming previous findings. The two populations had similar morphological responses to density and water availability in pure cultures and mixtures, but the population from the high-water environment showed a greater growth response to high water availability than did the population from the low-water environment and the difference in growth between the two populations decreased from the high-water to low-water treatment. Relative competitive ability of two populations were compared under three different water treatments and two densities. Differential response to watering treatment and density were not reflected in a difference in relative competitive ability. Relative yield totals were significantly greater than 1 overall. The niche differentiation suggested by RYTs>1 may be responsible for the lack of differential competitive effects observed for populations in the three vatering treatments.

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Optimization of Algerian Thymus fontanesii Boiss. & Reut Essential Oil Extraction by Electromagnetic Induction Heating

  • Ali, Lamia Sid;Brada, Moussa;Fauconnier, Marie-Laure;Kenne, Tierry
    • Natural Product Sciences
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    • v.24 no.1
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    • pp.71-78
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    • 2018
  • The present study deals with the determination of optimal values of operating parameters such as the temperature of heating, the mass of the plant material and the volume of water leading to the best yield of electromagnetic induction (EMI) heating extraction of Algerian Thymus fontanesii essential oil. After an appropriate choice of the three critical variables, eight experiments leaded to a mathematical model as a first-degree polynomial presenting the response function (yield) in the relation to the operating parameters. From the retained model, we were able to calculate the average response, the different effects and their interactions. The maximum of essential oil recovery percentage relative to the initial mass of plant material was 1.69%, and was obtained at ($140^{\circ}C$, 250 g and 4.5 L). The chemical composition of the Algerian T. fontanesii essential oil under the obtained optimal conditions ($140^{\circ}C$, 250 g and 4.5 L), determined by GC/MS and GC/FID, reveled of the presence of major components such as: carvacrol ($70.6{\pm}0.1%$), followed by p-cymene ($8.2{\pm}0.2%$).

Using oscillatory shear to probe the effects of bidispersity in inverse ferrofluids

  • Ekwebelam, C.C.;See, H.
    • Korea-Australia Rheology Journal
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    • v.19 no.1
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    • pp.35-42
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    • 2007
  • The effects of particle size distribution on the magnetorheological response of inverse ferrofluids was investigated using controlled mixtures of two monodisperse non-magnetisable powders of sizes $4.6\;{\mu}m\;and\;80{\mu}m$ at constant volume fraction of 30%, subjected to large amplitude oscillatory shear flow. In the linear viscoelastic regime (pre-yield region), it was found that the storage and loss moduli were dependent on the particle size as well as the proportion of small particles, with the highest storage modulus occurring for the monodisperse small particles. In the nonlinear regime (post yield region), Fourier analysis was used to compare the behaviour of the $1^{st}\;and\;3^{rd}$ harmonics ($I_{1}\;and\;I_{3}\;respectively$) as well as the fundamental phase angle as functions of the applied strain amplitude. The ratio of $I_{3}/I_{1}$ was found to become more pronounced with decreasing particle size as well as with increasing proportion of small particles in the bidisperse mixtures. Furthermore, the phase angle was able to clearly show the transition from solid-like to viscous behaviour. The results suggested that the nonlinear response of a bidisperse IFF is dependent on particle size as well as the proportion of small particles in the system.

Low-Complexity Detection Techniques for High-Density DVD Systems (고밀도 DVD시스템을 위한 저 복잡도 검출 기법)

  • Cho, Han-Gyu;Woo, Choong-Chae;Joo, Man-Sic;Kang, Chang-Eon;Hong, Dae-Sik
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.27 no.10A
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    • pp.1000-1010
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    • 2002
  • Partial response maximum-likelihood (PRML) and fixed-delay tree search with decision feedback (FDTS/DF) yield a sub-optimum performance in storage systems. However, they suffer from the inevitable complexity problems. this paper focuses on detection schemes to overcome the drawbacks of the sequence detections by exploiting minimum run-length d=2. It is expected that the proposed systems yield substantial reductions of both processing speed and receiver complexity. When combined with a decision feedback equalization (DFE), they prove to keep pace with the FDTS/DF with ${\tau}$=2 and even outperform the PR(1111)ML at normalized density S>5.6.

Optimization of Oil from Moringa oleifera seed using Soxhlet Extraction method

  • Ojewumi, M.E.;Oyekunle, D.T.;Emetere, M.E.;Olanipekun, O.O.
    • The Korean Journal of Food & Health Convergence
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    • v.5 no.5
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    • pp.11-25
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    • 2019
  • Extraction of oil from Moringa oleifera seed using Response Surface Methodology (RSM) was investigated. Effects of three factors namely: sample mass, particle size and extraction time on the response, Moringa oleifera a volume extracted, were determined. The Box-Behnken design of RSM was employed which resulted in 15 experimental runs. Extraction was carried out in a 250 ml Soxhlet extractor with Hexane and Ethanol as solvent. The Moringa oleifera seed powder was packed inside a muslin cloth placed in a thimble of the Soxhlet extractor. The extraction was carried out at 60℃ using thermostatic heating mantle. The solvent in the extracted oil was evaporated and the resulting oil further dried to constant weight in the oven. This study demonstrates that Moringa oleifera oil can be extracted from its seed using ethanol and acetone as extraction solvent. The optimum process variables for both solvent (ethanol and acetone) was determined at sample weight of 40 g, particle size of 325 ㎛ and extraction time of 8 hours. It can be deduced that using acetone as solvent produces a higher yield of oil at the same optimum variable conditions compared to when ethanol was used.

Production of Levulinic Acid Using Glucose Derived from Office Waste Paper (사무용 폐지에서 유래된 글루코오스를 이용한 레불린산 생산)

  • Ban, Se-Eun;Park, Yoon;Yi, Sung-Cho;Lim, Ye-Eun;Lee, Jae-Won
    • New & Renewable Energy
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    • v.17 no.2
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    • pp.32-39
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    • 2021
  • The optimal conditions for producing levulinic acid from office waste paper were investigated. Glucose was produced by enzymatic hydrolysis and its yield maximized by varying the soaking time of the substrate and amounts of enzyme and substrate. The optimal conditions to produce levulinic acid using the hydrolysate were determined by response surface methodology, with reaction temperature and catalyst (sulfuric acid) concentration as independent variables. The production model was assessed with an ANOVA regression analysis, and the results indicate its suitability for levulinic acid production (p, F, and lack-of-fit values were 0.003, 20.1, and 0.058, respectively). The optimal conditions were a reaction time of 56.27 min and catalyst concentration of 5.9% with a predicted yield of 2.588 g/L. We verified the findings under the same conditions and obtained 2.323 g/L of levulinic acid.

Effect of Graded Levels of Wheat Bran Supplementation on Intake, Nutrient Digestibility, Microbial N Yield and Growth Rate of Native Bulls Fed Rice Straw Alone

  • Chowdhury, S.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.11 no.2
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    • pp.162-170
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    • 1998
  • The dose response effects of different levels of wheat barn (WB) supplementation to a rice straw based diet has been studied in growing native (Bos indicus) bulls of Bangladesh. Twelve bulls of $266{\pm}29.6kg$ live weight and $32{\pm}9.5$ months old were given either of three diets of ad libitum untreated rice straw alone (T1) or straw supplemented with 2 (T2) or 3 (T3) kg WB. Besides, the experimental animals also received a mineral mixture. In 4 weeks, data were recorded on the intake, digestibility, microbial N (MN) yield, N balance (NB) and growth rate (GR). In the three diets, WB was 0 (T1), 29.6 (T2) and 42 (T3) percent of the total DM intake. With the increasing levels of WB supplementation, the total DM and OM intake increased, but the straw intake decreased linearly. WB supplementation increased the digestibilities of DM, OM and N but had no effect on ADF digestibility. The urinary purine derivative excretion and hence the MN yield increased with WB supplementation. The total MN yield were 7.26 and 35 g/d respectively for 0.2 and 3 kg WB supplementation. However, the efficiency of MN yield was highest (13 g/kg DOMR) at 2 kg WB level. Despite the increase DOM intake, the dietary ME content remain below 6 MJ/kg DM even at 3 kg WB supplementation. The NB were -84,467 and 1,620 mg/kg $W^{0.75}/d$ which were reflected on the GR of -186,346 and 554 g/d for 0, 2 and 3 kg WB supplementation respectively. Depending on the cost effectiveness, on an untreated rice straw diet, WB may be supplemented by up to 3 kg/d (42% of the diet) or more. However, if the maximum utilization of roughage is the main concern, the optimum WB level would probably be around 2 kg (30% of the diet) daily.

Effect of Sulphur and Nitrogen Application on Growth Characteristics, Seed and Oil Yields of Soybean Cultivars

  • Jamal Arshad;Fazli Inayat Saleem;Ahmad Saif;Abdin Malik Zainul;Yun Song Joong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.5
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    • pp.340-345
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    • 2005
  • A field experiment was conducted to assess the growth characteristics, seed and oil yield of two cultivars of soybean (G max (L.) Merr.) cv. PK-416 ($V_1$) and cv. PK-1024 ($V_2$) in relation to sulphur and nitrogen nutrition. Six combinations ($T_1-T_6$) of two levels of sulphur (0 and 40 kg $ha^{-1}$) and two levels of nitrogen (23.5 and 43.5 kg $ha^{-1}$) were applied to the two soybean cultivars as nutrients. Results indicated significant effect of sulphur and nitrogen, when applied together, on the growth characteristics, yield components, and seed and oil yield. Maximum response was observed with treatment $T_6$ (having 40 kg S and 43.5 kg N $ha^{-1}$). Seed and Oil yields were increased 90 and $102\%$ in $V_1$> and 104 and $123\%$ in $V_2$, respectively as compared to the control i.e. $T_1$ (having 0 kg S and 23.5 kg N $ha^{-1}$). Positive responses of S and N interaction on leaf area index, leaf area duration, crop growth rate and biomass production were also observed. The results obtained in these experiments clearly suggest that balanced and judicious application of nitrogen and sulphur can improve both seed and oil yield of soybean cultivars by enhancing their growth.