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http://dx.doi.org/10.13106/kjfhc.2019.vol5.no5.11

Optimization of Oil from Moringa oleifera seed using Soxhlet Extraction method  

Ojewumi, M.E. (Chemical Engineering Department, Covenant University)
Oyekunle, D.T. (Chemical Engineering Department, Covenant University)
Emetere, M.E. (Department of Physics, Covenant University)
Olanipekun, O.O. (Chemical Engineering Department, University of Lagos)
Publication Information
The Korean Journal of Food & Health Convergence / v.5, no.5, 2019 , pp. 11-25 More about this Journal
Abstract
Extraction of oil from Moringa oleifera seed using Response Surface Methodology (RSM) was investigated. Effects of three factors namely: sample mass, particle size and extraction time on the response, Moringa oleifera a volume extracted, were determined. The Box-Behnken design of RSM was employed which resulted in 15 experimental runs. Extraction was carried out in a 250 ml Soxhlet extractor with Hexane and Ethanol as solvent. The Moringa oleifera seed powder was packed inside a muslin cloth placed in a thimble of the Soxhlet extractor. The extraction was carried out at 60℃ using thermostatic heating mantle. The solvent in the extracted oil was evaporated and the resulting oil further dried to constant weight in the oven. This study demonstrates that Moringa oleifera oil can be extracted from its seed using ethanol and acetone as extraction solvent. The optimum process variables for both solvent (ethanol and acetone) was determined at sample weight of 40 g, particle size of 325 ㎛ and extraction time of 8 hours. It can be deduced that using acetone as solvent produces a higher yield of oil at the same optimum variable conditions compared to when ethanol was used.
Keywords
Moringa oleifera; RSM; Design of Experiment; Oil yield; Solvent; Soxhlet extraction;
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1 Abdulkarim, S. M., Lai, O. M., Muhammad, S. K. S., Long, K., & Ghazali, H. M. (2006). Use of enzymes to enhance oil recovery during aqueous extraction of Moringa oleifera seed oil. Journal of Food Lipids, 13, 113-130.   DOI
2 Abdulkarim, S. M., Long, K., Lai, O. M., Muhammad, S. K. S., & Ghazali, H. M. (2005). Some physico-chemical properties of Moringa oleifera seed oil extracted using solvent and aqueous enzymatic methods. Food Chemistry, 93, 253-263.   DOI
3 Abdulkarim, S. M., Long, K., Lai, O.M., Muhammad, S.K.S., Ghazali, H.M. (2007). Frying quality and stability of high-oleic Moringa oleifera seed oil in comparison with other vegetable oils, . Food Chem., 105, 1382-1389.   DOI
4 Anwar, F., Hussain, A. I., Iqbal, S., Bhanger, M.I. (2007). Enhancement of the oxidative stability of some vegetable oils by blending with Moringa oleifera oil. Food Chem., 103, 1181-1191.   DOI
5 Anwar, F., Latif, S., Ashraf, M., & Gilani, A. H. (2007). Moringa oleifera : A Food Plant with Multiple Medicinal Uses. Phytotherapy Research, 25, 17-25. doi:10.1002/ptr   DOI
6 Baoxin, Z., Xiangjing, W., and Wensheng, X. (2011). Optimization of fermentation medium for enhanced production of milbemycin by a mutant of Streptomyces bingchenggensis BC-X-1 using response surface methodology. African Journal of Biotechnology, 10(37), 7225-7235.
7 Bhutada, P. R., Jadhav, A. J., Pinjari, D. V, Nemade, P. R., & Jain, R. D. (2016). Solvent assisted extraction of oil from Moringa oleifera Lam. seeds. Industrial Crops & Products, 82, 74-80. doi:10.1016/j.indcrop.2015.12.004   DOI
8 Busani, M., Patrick Julius, Masika, Muchenje, V. (2012). Antimicrobial activity of Moringa oleifera (Lam.) root extract. Afr. J. Biotechnol., 11, 2797-2802.
9 Rashid, U., Anwar, F., Moser, B.R., Knothe, G. (2008). Moringa oleifera oil: a possible source of biodiesel. Bioresour. Technol., 99, 8175-8179.   DOI
10 Sudamalla, P., Saravanan, P., and Matheswaran, M. (2012). Optimisation of operating parameters using response surface methodology for adsorption of crystal violet by activated carbon prepared from mango kernel. Sustainable Environment Research, 22, 1-7.
11 Temitayo, O. D. (2017). Optimization of Oil Extraction from Thevetia Peruviana (Yellow Oleander) Seeds: A Case Study of Two Statistical Models. International Journal of Engineering and Modern Technology, 3(4), 2504-8856.
12 Tsaknis, J., Lalas, S., Gergis, V., & Spiliotis, V. (1998). A total characterization of Moringa oleifera Malawi seed oil. La Rivista Italiana delle Sostanze Grasse, 75, 21-27.
13 Upadhyay, P., Yadav, M.K., Mishra, S., Sharma, P., Purohit, S. (2015). Moringa oleifera: a review of the medical evidence for its nutritional and pharmacological properties. Int. J. Res. Pharm. Sci., 5, 12-16.
14 Yusoff, M. M., Gordon, M. H., Ezeh, O., & Niranjan, K. (2016). Aqueous enzymatic extraction of Moringa oleifera oil. Food Chemistry, 211, 400-408. doi:10.1016/j.foodchem.2016.05.050   DOI
15 Zhao, S., Zhang, D.,. (2013). A parametric study of supercritical carbon dioxide extraction of oil from Moringa oleifera seeds using a response surface methodology. Sep. Purif. Technol., 113, 9-17.   DOI
16 Fang, X. L., Feng, J. T., Zhang, W.G., Wang, Y. H., and Zhang, X. (2010). Optimization of growth medium and fermentation conditions for improved antibiotic activity of Xenorhabdus nematophila T B using a statistical approach. African Journal of Biotechnology, 9, 8068-8077.   DOI
17 Chabrand, R. M., Kim, H.-J., Zhang, C., Glatz, C. E., & Jung, S. (2008). Destabilization of emulsion formed during aqueous extraction of soybean oil. Journal of the American Oil Chemists Society, 85, 383-390.   DOI
18 Datta, D., Kumar, S. (2012). Modelling and optimisation of recovery process of glycolic acid using reactive extraction. International Journal of Chemical Engineering and Applications, 3, 141-146.   DOI
19 Fakayode, A. O., & Ajav, A. E. (2016). Process optimization of mechanical oil expression from Moringa (Moringa oleifera) seeds. Industrial Crops & Products, 90(142-151). doi:10.1016/j.indcrop.2016.06.017   DOI
20 Ghazali, H. M., Abdulkarim, S.M. (2011). In Moringa Oleifera Seed Oil: Composition, Nutritional Aspects and Health Attributes Elsevier Life Sciences (pp. 787-794).
21 Haider, M. A., Pakshirajan, K. (2007). Screening and optimization of media constituents for enhancing lipolytic activity by a soil microorganism using statistically designed experiments. Applied Biochemistry and Biotechnology, 141, 377-390.   DOI
22 Joglekar, M., and May, A. T. (1987). Product excellence through design of experiments. Cereal Foods World, 32, 857- 868.
23 Kafuku, G., Lam, M.K., Kansedo, J., Lee, K.T., Mbarawa, M. (2010). Heterogeneous catalyzed biodiesel production from Moringa oleifera oil. Fuel Process. Technol., 91, 1525-1529.   DOI
24 Latif, S., & Anwar, F. (2011). Aqueous enzymatic sesame oil and protein extraction. Food Chemistry, 125, 679-684.   DOI
25 Mahmood, K. T., Mugal, T., Haq, I.U., 2010. . (2010). Moringa oleifera: a natural gift-a review. J. Pharm. Sci. Res., 2, 775-781.
26 Latif, S., Anwar, F., Hussain, A. I., & Shahid, M. (2011). Aqueous enzymatic process for oil and protein extraction from Moringa oleifera seed. European Journal of Lipid Science and Technology, 113, 1012-1018.   DOI
27 Liu, G. C., Wang, X. L. (2007). Optimization of critical medium components using response surface methodology for biomass and extracellular polysaccharide production by Agaricus blazei. Applied Microbiology and Biotechnology, 74, 78-83.   DOI
28 Long, J. J., Fu, Y. J., Zu, Y. G., Li, J., Wang, W., Gu, C. B., & Luo, M. (2011). Ultrasound- assisted extraction of flaxseed oil using immobilized enzymes. Bioresource Technology, 102, 9991-9996.   DOI
29 Martins, P. F., Melo, M. M. R. De, & Silva, C. M. (2016). Techno-economic optimization of the subcritical fluid extraction of oil from Moringa oleifera seeds and subsequent production of a purified sterols fraction. The Journal of Supercritical Fluids, 107, 682-689. doi:10.1016/j.supflu.2015.07.031   DOI
30 Mofijur, M., Masjuki, H.H., Kalam, M.A., Atabani, A.E., Arbab, M.I., Cheng, S.F., Gouk, S.W. (2014). Properties and use of Moringa oleifera biodiesel and diesel fuel blends in a multi-cylinder diesel engine. Energy Convers. Manage, 82, 169-176.   DOI
31 Mofijur, M., Masjuki, H.H., Kalam, M.A., Atabani, A.E., Fattah, I.M.R., Mobarak, H.M. (2014). Comparative evaluation of performance and emission characteristics of Moringa oleifera and palm oil based biodiesel in a diesel engine. Ind. Crops Prod., 53, 78-84.   DOI
32 Muyibi, S. A., & Evison, L. M. (1995). Moringa oleifera seeds for softening. Water Resources, 29(4), 1099-1105.
33 Ojewumi, M. E., Emetere, M. E., Babatunde, D. E., & Okeniyi, J. O. (2017b). In Situ Bioremediation of Crude Petroleum Oil Polluted Soil Using Mathematical Experimentation. International Journal of Chemical Engineering, Volume 2017c, Article ID 5184760, 2017, doi:10.1155/2017/5184760.
34 Nguyen, H. N., Gaspillo, P. D., Maridable, J. B., Malaluan, R. M., Hinode, H., Salim, C., & Huynh, H. K. P. (2011). Extraction of oil from Moringa oleifera kernels using supercritical carbon dioxide with ethanol for pretreatment : Optimization of the extraction process. Chemical Engineering & Processing: Process Intensification, 50(11-12), 1207-1213. doi:10.1016/j.cep.2011.08.006   DOI
35 Ojewumi, M.E. (2016). Optimizing the Conditions and Processes for the Production of Protein Nutrient from Parkia biglobosa Seeds. Dissertation submitted In partial fulfillment for the award of Ph.D in the Departemnt of Chemical Engineering, Covenant University, Nigeria.
36 Ojewumi, M. E., Eluagwule, B., Ayoola, A. A., Ogunbiyi, A. T., Adeoye, J., Emetere, M.E., & Joseph, O. O. (2017a). Termiticidal effects of african locust bean (Parkia biglobosa) seed oil extracts. International Journal of Current Research, 53929-53934.
37 Ojewumi, M. E., Adedokun, S. O., Omodara, O. J., Oyeniyi, E. A., Taiwo, O. S., & Ojewumi, E. O. (2017c). Phytochemical and Antimicrobial Activities of the Leaf Oil Extract of Mentha Spicata and its Efficacy in Repelling Mosquito. International Journal of Pharmaceutical Research & Allied Sciences, 6(4), 17-27.
38 Ojewumi, M. E., Banjo, M. G., Oresegun, M. O., Ogunbiyi, T. A., Ayoola, A. A., Awolu, O. O., & Ojewumi, E. O. (2017d). Analytical Investigation of the Extract of Lemon Grass Leaves in Repelling Mosquito. International Journal of Pharmaceutical Sciences and Research, 8(5), 2048-2055. doi:10.13040/ijpsr.0975-8232.8(5).2048-55.
39 Ojewumi, M.E., Olikeze, F., Babatunde, D.E., Emetere, M.E. (2018a). Alternative Solvent Ratios for Moringa Oleifera Seed Oil Extract. International Journal of Mechanical Engineering and Technology, 9(12), 295-307.
40 Ojewumi, M.E., Omoleye, J.A., Ajayi, A.A. (2017e). Optimization of Fermentation Conditions for the Production of Protein Composition in Parkia biglobosa Seeds using Response Surface Methodology. International Journal of Applied Engineering Research, 12(22), 12852-12859.
41 Ojewumi, M.E., Kayode, G.O., Omoleye, J.A., Oyekunle, D.T. (2019). Statistical optimization and sensitivity analysis of rheological models using cassava starch. International Journal of Civil Engineering and Technology (IJCIET), 10(01), 623-639, Article ID: IJCIET_10_01_057 http://www.iaeme.com/ijciet/issues.asp?JType=IJCIET&VType=10&IType=01
42 Ojewumi, M. E., Adeyemi, A. O., & Ojewumi, E. O. (2018b). Oil extract from local leaves - An Alternative to synthetic mosquito repellants. Pharmacophore, 9(2), 1-6.
43 Ojewumi, M. E., Oyeyemi, K. G., Emetere, M. E., & Okeniyi, J. O. (2018c). Data on the rheological behavior of cassava starch paste using different models. Data in Brief, 19, 2163-2177.   DOI
44 Ojewumi, M.E., Adedokun, F.S., Ayoola, A.A., Taiwo, S.O. (2018d). Evaluation of the oil Extract from Mentha spicata and its Chemical Constituents. International Journal of Sciences and Research, 74 (11/1). DOI: 10.21506/j.ponte.2018.11.7.
45 Ojiako, E. N., Okeke, C.C. (2013). Determination of antioxidant of Moringa oleifera seed oil and its use in the production of a body cream. Asian J. Plant Sci. Res., 3, 1-4.
46 Oyekunle, D. T., & Oyekunle, D. O. (2018). Biodiesel production from yellow oleander seed oil via heterogeneous catalyst : performance evaluation of minitab response surface methodology and artificial neural network. Journal of Materials and Environmental Sciences, 9(8), 2468-2477.
47 Panwal, J. H., Viruthagiri, T. and Baskar, G. (2011). Statistical modeling and optimization of enzymatic milk fat splitting by soybean lecithin using response surface methodology. International Journal of Nutrition and Metabolism, 3, 50-57.
48 Patel, S., Kothari, D., and Goyal, A. (2011). Enhancement of dextransucrase activity of Pediococcus pentosaceus SPAm1 by Response Surface Methodology. Indian Jour-nal of Biotechnology, 10, 346-351.