• Asian Pacific Journal of Cancer Prevention
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• 제14권8호
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• pp.4615-4619
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• 2013

Background: Artesunate, extracted from Artemisia annua, has been proven to have anti-cancer potential. Allicin, diallyl thiosulfinate, the main biologically active compound derived from garlic, is also of interest in cancer treatment research. This object of this report was to document synergistic effects of artesunate combined with allicin on osteosarcoma cell lines in vitro and in vivo. Methods: After treatment with artesunate and allicin at various concentrations, the viability of osteosarcoma cells was analyzed by MTT method, with assessment of invasion and motility, colony formation and apoptosis. Western Blotting was performed to determine the expression of caspase-3/9, and activity was also detected after drug treatment. Moreover, in a nude mouse model established with orthotopic xenograft tumors, tumor weight and volume were monitored after drug administration via the intraperitoneal (i.p.) route. Results: The viability of osteosarcoma cells in the combination group was significantly decreased in a concentration and time dependent manner; moreover, invasion, motility and colony formation ability were significantly suppressed and the apoptotic rate was significantly increased through caspase-3/9 expression and activity enhancement in the combination group. Furthermore, suppression of tumor growth was evident in vivo. Conclusion: Our results indicated that artesunate and allicin in combination exert synergistic effects on osteosarcoma cell proliferation and apoptosis.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.1911-1915
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• 2012

Aim: New technologies for the early detection of pancreatic cancer (PC) are urgently needed. The aim of the present study was to screen for the potential protein biomarkers in serum using proteomic fingerprint technology. Methods: Magnetic beads combined with surface-enhanced laser desorption/ionization (SELDI) TOF MS were used to profile and compare the protein spectra of serum samples from 85 patients with pancreatic cancer, 50 patients with acute-on-chronic pancreatitis and 98 healthy blood donors. Proteomic patterns associated with pancreatic cancer were identified with Biomarker Patterns Software. Results: A total of 37 differential m/z peaks were identified that were related to PC (P < 0.01). A tree model of biomarkers was constructed with the software based on the three biomarkers (7762 Da, 8560 Da, 11654 Da), this showing excellent separation between pancreatic cancer and non-cancer., with a sensitivity of 93.3% and a specificity of 95.6%. Blind test data showed a sensitivity of 88% and a specificity of 91.4%. Conclusions: The results suggested that serum biomarkers for pancreatic cancer can be detected using SELDI-TOF-MS combined with magnetic beads. Application of combined biomarkers may provide a powerful and reliable diagnostic method for pancreatic cancer with a high sensitivity and specificity.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 한국방송공학회 2009년도 IWAIT
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• pp.89-93
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• 2009

Embedded systems are becoming more popular as many embedded platforms have become more affordable. It offers a compact solution for many different problems including computer vision applications. Texture classification can be used to solve various problems, and implementing it in embedded platforms will help in deploying these applications into the market. This paper proposes to deploy the texture classification algorithms onto the embedded computer vision (ECV) platform. Two algorithms are compared; grey level co-occurrence matrices (GLCM) and Gabor filters. Experimental results show that raw GLCM on MATLAB could achieves 50ms, being the fastest algorithm on the PC platform. Classification speed achieved on PC and ECV platform, in C, is 43ms and 3708ms respectively. Raw GLCM could achieve only 90.86% accuracy compared to the combination feature (GLCM and Gabor filters) at 91.06% accuracy. Overall, evaluating all results in terms of classification speed and accuracy, raw GLCM is more suitable to be implemented onto the ECV platform.

• Macromolecular Research
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• 제11권3호
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• pp.146-151
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• 2003

The dependence of electrical conductivity on concentrations of diallyldimethylammonium chloride (DADMAC) monomer, linear poly(DADMAC) and their mixture monomer/poly(DADMAC) in dilute aqueous solution exhibits a linear relationship. It was possible to calculate conversion of DADMAC polymerization by measuring its electric conductivity. Although the electrical conductivity of the poly(DADMAC) solution decreased with increasing its molecular weight, in the process of UV or ionizing radiation polymerization the molecular weight of the polymers could be kept constant in the case of fixed temperature, UV-luminous intensity or dose rate. Based on the method mentioned above, the kinetics of UV induced polymerization of DADMAC in aqueous solution was studied; the overall activation energy of polymerization of DADMAC in the water phase was calculated to be 18.8 kJ mol$^{-1}$ . ${\gamma}$-Radiation-induced polymerization of DADMAC in aqueous solution as a function of absorbed dose was studied as well. The conversion of DADMAC increased quickly with dose before 30 kGy and then increased slowly. The experimental data of both UV- and ${\gamma}$-induced polymerization were verified to be reliable by inverted ultracentrifugation method.

• Herbal Formula Science
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• 제17권2호
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• pp.143-150
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• 2009

The effects of aqueous extract of Schizandrae Fructus (AESC) on lead (Pb)-induced changes of monoamine neurotransmitters in the hippocampus (HIP) of adult rats were investigated. Male Sprague-Dawley rats were received intraperitoneal (i.p.) administration of Pb acetate (5 mg/kg/d) for 28 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) in HIP were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Pb treated rats (P < 0.05), while pretreatment with AESC (100 mg/kg/d or 300 mg/kg/d, p.o., 2 h before Pb) greatly inhibited the decrease of monoamine transmitters, respectively (P < 0.05). Also, AESC (300 mg/kg/d) significantly increased the reduction of glutathione contents and superoxide dismutase activities in HIP induced by chronic Pb. These results suggest that AESC ameliorates Pb-induced depletion of monoamine neurotransmitters in HIP through its antioxidant activity.

• Proceedings of the Korean Society for Language and Information Conference
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• 한국언어정보학회 2007년도 정기학술대회
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• pp.522-530
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• 2007

This article investigates the use of distal demonstrative Hitlo in Taiwanese Southern Min (TSM) from a discourse-pragmatic perspective. The analysis is based on a 5-hour corpus of spoken data, including daily conversations, radio interviews, TV drama series, and some random examples. A total of 172 tokens of Hitlos are identified in the data. They can be divided into six categories according to their functions: firstly, exophoric usage, those Hitlos which refer to an object non-linguistically which can be identified in the immediate situation; secondly, endophoric usage, those which refer to an element textually; thirdly, referent introducing function, those which can be used to introduce a new but identifiable referent into the conversation (the referent usually has topical importance); fourthly, hedging expression, those which serve as a marker of imprecision; fifthly, a condition introducing marker, those which function as an indicator of the coming of a conditional sentence; finally, pause fillers, those which help speakers to manage speech turn or indicate the mental states In addition, an interactive function which Hitlo is found to serve will be discussed. Moreover, a grammaticalizational process involving semantic bleaching which Hitlo is probably undergoing is revealed in general. Finally, a filled demonstrative principle, stating that it may be a universal phenomenon to use demonstratives as filled pause will be proposed.

• Proceedings of the SAREK Conference
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• 대한설비공학회 2008년도 하계학술발표대회 논문집
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• pp.1309-1314
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• 2008

I.G.G is abbreviation for Inert Gas Generator, High temperature in Cargo Tank it desulfurize, exhausted and froze the gas that combined brimstone element and soot, then supply Inert gas by blower, and mack tank inside incombustible range this is equipment that nip in the bud the explosion. The blower for suppling inactivated gas has big impeller with heavy weight to achieve the high pressure, it causes a delay for first operation time and too much load is delivered to motor, total destruction by fire of motor is happening frequently. On this research, we will reduce the size & weight of impeller and install it with several stage, it makes an effect for reducing the first operation time. We also intend to contribute to efficient IGG blower design by research a flowing & pressure specialty from the diameter of impeller, number of blade, and size of casing.

• The Journal of Information Systems
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• 제29권1호
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• pp.113-136
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• 2020

Purpose In recent years, "Internet + " is a new social form. Has already brought the vitality of social and economic entities. The rapid development of Internet globalization, promote the development of an emerging network interaction, "Live broadcast". Viewers from all over the world can watch various types of live programs by connecting to the Internet. Viewers and live broadcasters can generate instant and efficient interactions. Design Many companies have taken advantage of the advantages of the live broadcast platform. Use live broadcasts for brand marketing activities. Branding compared to offline, online advertising is cheaper, Spread more quickly, get feedback from consumers more realistic. Merchants are also aware that this type of interaction creates a closer connection between consumers and businesses. This article will deduce a unique research model through lots of prior studies. Establish independent variables from two aspects of live software features and consumer features, and from the seven hypotheses derived, summarize how to make consumers more loyal to the same brand. The audience of Korean and Chinese live broadcast software is also growing. In order compare the differences between the consumer groups in Korea and China, this article uses the same research model, analysis of consumers in Korea and China. Findings Finally based on the results of the study. Proposal for rationalization of companies that use Korean-Chinese live broadcast platforms for brand marketing.

• Archives of Pharmacal Research
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• 제28권4호
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• pp.395-399
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• 2005

From $70\%$ ethanol extract of the roots of Smilax bockii warb., seven flavonoids, kaempferol (1), $kaempferol-7-O-\beta-D-glucopyranoside$ (2), quercetin (3), isorhamnetin (4), (+)-dihydro­kaempferol (5), engeletin (6), isoengeletin (7), and $n-butyl-\beta-D-fructopyranoside$ (8), caffeic acid n-butyl ester (9) were isolated and identified by means of chemical and spectroscopic. Compounds 2, 4, and 6-9 were isolated for the first time from the roots of S. bockii and compounds 2, 8, and 9 were firstly isolated from the genus Smilax. In addition, using the SEAP (Secreted alkaline phosphatase) assay system, we investigated the in vitro anti-inflammatory activity of the $70\%$ ethanol extract of the roots of S. bockii, which showed moderate activity in inhibiting $TNF-\alpha-induced NF-{\kappa}B$ activation with an $IC_{50}$ value of $166.6 {\mu}g/mL$.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권2호
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• pp.122-127
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• 2005

A size exclusion chromatography (SEC) process, in the presence of denaturant in the refolding buffer was developed to refold recombinant human $interferon-\gamma$ ($rhIFN-\gamma$) at a high concentration. The $rhlFN-\gamma$ was overexpressed in E. coli resulting in the formation of inactive inclusion bodies (IBs). The IBs were first solubilized in 8 M urea as the denaturant, and then the refolding process performed by decreasing the urea concentration on the SEC column to suppress protein aggregation. The effects of the urea concentration, protein loading mode and column height during the refolding step were investigated. The combination of the buffer-exchange effect of SEC and a moderate urea concentration in the refolding buffer resulted in an efficient route for producing correctly folded $rhIFN-\gamma$, with protein recovery of $67.1\%$ and specific activity up to $1.2\times10^7\;IU/mg$.