Refolding and Purification of Recombinant Human Expressed as Inclusion Bodies in Escherichia coli Using Size Exclusion Chromatography |
Guan Yi-Xin
(Department of Chemical and Biochemical Engineering, Zhejiang University)
Pan Hai-Xue (Department of Chemical and Biochemical Engineering, Zhejiang University) Gao Yong-Gui (Department of Chemical and Biochemical Engineering, Zhejiang University) Yao Shan-Jing (Department of Chemical and Biochemical Engineering, Zhejiang University) Cho Man-Gi (Advanced Science and Technology Research Center, Graduate School of Biotechnology, Dongseo University) |
1 | Lilie, H., E. Schwarz, and R. Rudolph (1998) Advances in refolding of proteins produced in E. coli. Curr. Opin. Biotechnol. 9: 497-501 DOI ScienceOn |
2 | Cho, T. H., S. J. Ahn, and E. K. Lee (2002) Refolding of protein inclusion bodies directly from E. coli homogenate using expanded bed adsorption chromatography. Bioseparation 10: 189-196 DOI ScienceOn |
3 | Creighton, T. E. (1990) US Patent 4,977,248 |
4 | Batas, B. and J. B. Chaudhuri (1999) Considerations of sample application and elution during size-exclusion chromatography-based protein refolding. J. Chromatogr. A. 864: 229-236 DOI PUBMED ScienceOn |
5 | Fahey, E. M., J. B. Chaudhuri, and P. Binding (2000) Refolding and purification of a urokinase plasminogen activator fragment by chromatography. J. Chromatogr. B. 737: 225-235 DOI ScienceOn |
6 | Rudolph, R. and H. Lilie (1996) In vitro folding of inclusion body proteins. FASEB J. 10: 49-56 |
7 | Gao, Y. G., Y. X. Guan, S. J. Yao, and M. G. Cho (2003) On-column refolding of recombinant human interferon- with an immobilized chaperone fragment. Biotechnol. Prog. 19: 915-920 DOI PUBMED ScienceOn |
8 | Pestka, S., A. J. Langers, C. K. Zoon, and C. E. Samuel (1987) Interferons and their actions. Annual Review Biochemistry 56: 727-777 DOI ScienceOn |
9 | De Bernardez Clark, E. (2001) Protein refolding for industrial processes. Curr. Opin. Biotechnol. 12: 202-207 DOI PUBMED ScienceOn |
10 | Rogl, H., K. Kosemund, W. Kuhlbrandt, and I. Collinson (1998) Refolding of Escherichia coli produced membrane protein inclusion bodies immobilised by nickel chelating chromatography. FEBS Letters 432: 21-26 DOI PUBMED ScienceOn |
11 | Zhang, D. Z., S. H. Wu, Y. D. Hou, and C. Z. Su (1989) The purification of recombinant human interferon-. Chinese J. Virology 5: 37-40 |
12 | Bradford, M. M. (1976) A rapid and sensitive method for the quantization of protein utilizing the principles of protein- dye binding. Anal. Biochem.72: 248-254 DOI PUBMED ScienceOn |
13 | Kim, C. S. and E. K. Lee (2000) Effects of operating parameters in in vitro renaturation of a fusion protein of human growth hormone and glutathiones transferase for inclusion body. Process Biochemistry 36: 111-117 DOI ScienceOn |
14 | Gu, Z. Y., Z. G. Su, and J. C. Janson (2001) Urea gradient size-exclusion chromatography enhanced the yield of lysozyme refolding. J. Chromatogr. A. 918: 311-318 DOI PUBMED ScienceOn |
15 | Li, M., G. F. Zhang, and Z. G. Su (2002) Dual gradient ion-exchange chromatography improved refolding yield of lysozyme. J. Chromatogr. A. 959: 113-120 DOI PUBMED ScienceOn |
16 | Lanckriet, H. and A. P. J. Meddelberg (2004) Continuous chromatographic protein refolding. J. Chromatogr. A. 1022: 103-113 DOI PUBMED ScienceOn |
17 | Gao, Y. G., Y. X. Guan, S. J. Yao, and M. G. Cho (2003) Lysozyme refolding at high concentration by dilution and size-exclusion chromatography. J. Zhejiang University Science 4: 136-141 DOI ScienceOn |
18 | Cui, Z. F., Y. X. Guan, and S. J. Yao (2004) A temperature- sensitive hydrogel refolding system: Preparation of poly(N-isopropyl acrylamide) and its application in lysozyme refolding. Chinese J. Chem. Eng. 12: 556-560 |
19 | Rudlph, R., G. Bohm, H. Lilie, and R. Jaenicke (1997) In Protein Function: A Practical Approach. 2nd ed., pp. 57-99. IRL Press, Oxford, UK |
20 | Du, P (1984) Medical Interferon. pp. 231-307. Liberation Army Publishing House, Beijing, China |
21 | Gao, Y. G., Y. X. Guan, S. J. Yao, and M. G. Cho (2002) Refolding of lysozyme at high concentration in batch and fed-batch operation. Korean J. Chem. Eng. 19: 871-875 DOI ScienceOn |
22 | Geng, X. D. and X. Q. Chang (1992) High-performance hydrophobic interaction chromatography as a tool for protein refolding. J. Chromatogr. A. 599: 185-194 DOI ScienceOn |
23 | Laemmli, U. K. (1970) Detection of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685 DOI PUBMED ScienceOn |
24 | Park, S. J., K. Ryu, C. W. Suh, Y. G. Chai, O. B. Kwon, S. K. Park, and E. K. Lee (2002) Solid-phase refolding of poly-lysine tagged fusion protein of hEGF and angiogenin. Biotechnol. Bioprocess Eng. 7: 1-5 DOI ScienceOn |