• 한국터널지하공간학회 논문집
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• 제22권6호
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• pp.623-641
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• 2020

터널식 전력구는 약 5 km 이내의 연장을 기계식 굴착장비인 밀폐형 쉴드TBM을 이용하여 건설된다. 공기지연 예방 및 적기가압을 위해서는 암반등급별 공사기간의 예측정확도를 향상시켜야하며, 투입된 장비의 성능을 고려한 최적운전이 필수적이다. 이에 본 연구에서는 3.6 m급 쉴드TBM에 적합한 순굴진율 모델을 개발하고자 실대형 굴진시험을 수행하였다. 대표적인 일축압축강도에 대해서 소요추력과 커터헤드 회전속도를 바탕으로 약 100회의 실험을 수행하였다. 이에 풍화암, 연암, 경암분류에 따라 일축압축강도와 장비의 소요추력 조건이 고려된 연직력과 압입깊이 및 일축압축강도와 토크와의 상관관계를 제시하였다. 개발된 순굴진율 모델을 바탕으로 구동부 성능분석과 최적운전 조건에 대해 제시하였다. 본 연구결과를 통해 터널식 전력구 공사기간 단축 및 조기사용에 대한 사용자 편익증대를 기대할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.746-753
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• 2008

The hprK gene encoding bifunctional HPrK/P (kinase/phosphorylase) was cloned from L. mesenteroides SY1, a strain isolated from kimchi. hprK was transcribed as a monocistronic gene. His-tagged HPrH16A and HPrK/P were produced in E. coli BL21 (DE3) using pET26b(+) and purified. HPrK/P phosphorylation assay with purified proteins showed that the kinase activity of HPrK/P increased at slightly acidic pHs. Divalent cations such as $Mg^{2+}$ and $Mn^{2+}$ and glycolytic intermediates such as fructose-1, 6-bisphosphate (FBP) and phosphoenolpyruvate (PEP) increased the kinase activity of HPrK/P, but inorganic phosphate strongly inhibited it. Kinetic studies for the kinase activity of HPrK/P showed that the apparent $K_m$ values were 0.18 and $14.57{\mu}M$ for ATP and HPr, respectively. The $K_m$ value for the phosphorylase activity of HPrK/P was $14.16{\mu}M$ for P-Ser-HPr (HPr phosphorylated at the serine residue).

• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.573-578
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• 2008

Lactobacillus paraplantarum KM (Lp), Weissella sp. 33 (Ws), and Enterococcus faecium 35 (Ef) were used in single (Lp, Ws, Ef) or mixed cultures (Lp+Ws, Lp+Ef, Ws+Ef) for soy milk fermentation ($37^{\circ}C$, 12 h). After 12 h, the cell numbers, pH, and TA of soymilk were $7.4{\times}10^8-6.0{\times}10^9CFU/ml$, 3.8-4.5, and 0.59-0.70%, respectively. Changes in the contents of glycitin and genistin in soymilk fermented with Ef were not significant. The contents of isoflavone glucosides in soymilk fermented with the other cultures decreased significantly with an increase of aglycone contents (p<0.05). It corresponded well with a sharp increase in ${\beta}$-glucosidase activity during fermentation. About 92-100% of the daidzin and 98-100% of the genistin in soymilk were converted to corresponding aglycones by Lp, Ws, or Lp+Ef within 12 h.

• Bulletin of the Korean Chemical Society
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• 제31권2호
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• pp.303-308
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• 2010

Pseudo-first-order rate constants ($k_{obsd}$) have been measured spectrophotometrically for nucleophilic substitution reactions of 4-nitrophenyl benzoate (5a), 4-nitrophenyl 4-methoxybenzoate (5b), and 4-nitrophenyl 4-hydroxybenzoate (5c) with alkali metal ethoxides, $EtO^-M^+$ ($M^+=Li^+$, $Na^+$ and $K^+$) in anhydrous ethanol (EtOH) at $25.0{\pm}0.1^{\circ}C$. The plots of $k_{obsd}$ vs. [$EtO^-M^+$] exhibit upward curvatures in all cases, indicating that $M^+$ ions catalyze the reactions and ionpaired $EtO^-M^+$ species are more reactive than dissociated $EtO^-$. Second-order rate constants for reactions with dissociated $EtO^-$ and ion-paired $EtO^-M^+$ (i.e., $k_{EtO^-}$ and $k_{EtO^-M^+}$, respectively) have been calculated from ion-pair treatment for the reactions of 5a and 5b. However, such ion-pair treatment has failed to determine $k_{EtO^-}$ and $k_{EtO^-M^+}$ values for the reactions of 5c. It has been concluded that reactions of 5a and 5b are catalyzed by one metal ion, which increases electrophilicity of the reaction center through coordination on the carbonyl oxygen. In contrast, reactions of 5c have been suggested to involve two metal ions, i.e., the one coordinated on the carbonyl oxygen increases the electrophilicity of the reaction center while the other one associated on the phenoxy oxygen decreases the charge repulsion between the anionic reagents (i.e., $EtO^-$ and deprotonated 5c). It has been found that the rate equation derived from the mechanism involving two metal ions fits nicely to the kinetic results obtained for the reactions of 5c.

• 동아시아식생활학회지
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• 제25권5호
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• pp.813-821
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• 2015

Asthma is an increasing global health problem, and novel strategies to prevent or ameliorate the condition are needed. The purpose of the present study was to evaluate the anti-inflammatory and anti-asthma effects of Adenophora triphylla var. japonica extracts. We investigated the molecular mechanism underlying the effects of 80% ethanol extracts (AE) of A. triphylla on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. AE treatment inhibited pro-inflammatory cytokines such as TNF-${\alpha}$ and IL-6 as well as nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells. In particular, NO and pro-inflammatory cytokine production was suppressed more effectively by aerial parts (AE-A) than roots (AE-R) of A. triphylla. Quantitative RT-PCR assay showed that AE reduced mRNA levels of iNOS and COX-2. We also evaluated the anti-asthmatic effects of AE-A in an ovalbumin (OVA)-induced BALB/c mouse model. AE-A supplementation significantly reduced the amounts of airway eosinophils, IL-4 and IL-13 levels in BALF, and IgE levels in serum as compared with untreated, OVA-induced mice. These results suggest that AE-A can be considered as a therapeutic agent to potentially relieve asthma.

• 대한간호학회지
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• 제45권6호
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• pp.939-948
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• 2015

Purpose: An emerging trend in Asian higher education is English-medium instruction (EMI), which uses English as the primary instructional language. EMI prepares domestic students for international leadership; however, students report difficulty in learning, and educators have raised questions concerning the effectiveness of EMI. The flipped learning model (FLM), in which lecture and homework activities for a course are reversed, was applied to an English-medium course offered by a college of nursing in Korea. The aims of this study were to: 1) revise an existing English-medium nursing course using the FLM; 2) explore students' learning experiences and their acceptance of the FLM; and 3) identify key factors in the success of FLM. Methods: We used a descriptive, cross-sectional, mixed-methods design and the participants were students at one nursing school in Korea. A series of course development meetings with faculties from the nursing school and the center for teaching and learning were used to develop the course format and content. We conducted course evaluations using the Flipped Course Evaluation Questionnaire with open-ended questions and focus group interviews. Results: Students (N=75) in a 15-week nursing course responded to a survey after completing the course. Among them, seven students participated in one of two focus groups. Overall, students accepted and favored the flipped learning strategy, and indicated that the method enhanced lecture content and their understanding of it. Factors associated with effective instruction included structured monitoring systems and motivational environments. Conclusion: The FLM requires sufficient preparation to facilitate student motivation and maximize learning outcomes.

• 한국해양공학회지
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• 제28권4호
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• pp.294-305
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• 2014

In offshore structures, fire is one of the most important hazardous events. The concern of fires has recently been reflected in the rules and quantified risk assessment based design practice. Within the framework of quantified risk assessment and the management of offshore installations, therefore, more refined computations of the consequences or hazardous action effects due to fire are required. To mitigate fire risk, passive fire protection(PFP) is widely used on offshore structures. This study presents methods for a nonlinear structural response analysis considering the PFP effects under fires. It is found that a structural response analysis is most likely to use valuable technology for the optimization and design of offshore structures with PFP. Thermal and structural response analyses have been performed using LS-DYNA and FAHTS/USFOS. The results of these structural response analyses are compared with each other.

• 한국지반환경공학회 논문집
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• 제3권3호
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• pp.45-51
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• 2002

신축 매립장 부지내 절개사면에서 유출되고 있는 유출수가 인접한 기존의 폐기물 매립장으로부터의 침출수 유입여부를 평가하는데 있다. 판단은 유출되고 있는 유출수의 수질을 기존매립장의 침출수, 감시정 그리고 신축부지 근처의 지하수와 하천수와 비교하였고, 침출수의 여과 실험과 유출수의 유출지점에서 발생되는 악취에 대한 $NH_3$와 $H_2S$분석도 같이 수행하였다. 공사장내 유출수의 오염도가 상대적으로 높은값을 나타내었고, 유출수의 유기물 미량분석과 악취분석도 침출수에서 발견되는 유기물이 검출된 결과를 보여주어, 유출수는 인접한 폐기물 매립장으로부터 침출수가 유입되는 것으로 판단되며 공사시 이에 대한 대책이 선행이 되어야 된다고 사료된다.

• 생약학회지
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• 제41권2호
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• pp.136-140
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• 2010

This study was carried out to utilize non-toxic solvents instead of harmful solvents for the TLC(Thin Layer Chromatography) identification test of herbal medicines. It is recommended not to use harmful solvent such as chloroform at a viewpoint of clean analysis. In this study, we revised the identification test of 10 items in the Korea Pharmacopoeia(KP) and the Korean Herbal Pharmacopoeia(KHP) such as Cornus officinalis S. containing the harmful solvents on the developing solvent and established identification test that is utilized non-toxic solvents by HPTLC(High Performance Thin Layer Chromatography).

• 한국임상수의학회지
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• 제29권6호
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• pp.506-508
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• 2012

5년령 중성화하지 않은 암컷 포메라니언이 닭뼈를 삼킨 후 구토를 주증으로 내원하였다. 내시경을 통한 식도이물 제거 후 식도에 작은 천공을 확인하였다. 수술적 치료 대신 비수술적 관리를 통하여 치료하였고 3주 후 내시경을 통한 재검사 결과 천공부위의 완전한 폐쇄를 확인하였다. 본 연구는 식도 이물에 의한 식도 천공을 비수술적 관리를 통해 성공적으로 치료한 증례에 대한 보고이다.