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Effect of Thiol Compounds and Antioxidants on In Vitro Development and Intracellular Glutathione Concentrations of Bovine Embryos Derived from In Vitro Matured and In Vitro Fertilized II. Effect of Antioxidants with Somatic Cells on Development and Intracellular Glutathione Concentrations of Bovine IVM/IVF Embryos (Thiol 화합물과 항산화제 첨가배양이 소 체외수정란의 체외발육과 세포내 Glutathione 농도 변화에 미치는 효과 II. 항산화제 첨가와 체세포 공동배양이 소 체외수정란의 체외발육과 세포내 Glutathione 농도 변화에 미치는 영향)

  • 양부근;박동헌;우문수;정희태;박춘근;김종복;김정익
    • Korean Journal of Animal Reproduction
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    • v.21 no.4
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    • pp.345-353
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    • 1997
  • Antioxidants and antioxidants with somatic cell co-culture, bovine oviduct epithelial cells(BOEC) and buffalo rat liver cells(BRLC), were studied as a mean of increasing the development and intracellular glutathione(GSH) concnetrations of bovine embryos derived from in vitro matured(IVM) and in vitro fertilized(IVF) oocytes. Cell numbers and intracellular GSH concentrations of blastocysts were also counted. The developmental rate beyond morula stages in CRlaa containing taurine(2.5mM), superoxide dismutase(SOD, 600U) and catalase(250U) were 1%, 75.0%, 64.8% and 62.3%, respectively. The developmental rate in antioxidant groups was significantly higher than in control(P<0.05). The intracellular GSH concentrations of blastocysts cultured in 0, 2.5mM taurine, 600U SOD and 250U catalase were 33.8pM, 39.3pM, 42.3pM and 54.8pM, respectively. This result indicated that the developmental rates and intracellular GSH concentrations of catalase group was significantly higher than any other groups(P<0.05). The developmental capacity in CRlaa plus various antioxidants co-cultured with BOEC were 55.3%(control), 74.1%(2.5mM taurine), 66.7%(600U SOD) and 70.7%(250U catalase) and in CRlaa plus various antioxidants co-cultured with BRLC in control, 2.5mM taurine, 600U SOD and 250U catalase were 63.8%, 75.5%, 71.0% and 73.5%, respectveily, the intracellular GSH concentrations of blastocyst embryos co-cultured with BOEC and BRLC in CRlaa with 0.25mM taurine, 600U SOD and 250U catalase were 73.4pM and 64.4pM, 79.9pM and 67.5pM, 82.3pM and 71.7pM, and 83.0pM and 80.0pM, respectively. Cell numbers of blastocysts were not difference in all experimental groups. These studies indicate that andtioxidants and antioxidant with somatic cell co-culture can increase the proportion of embryo that developed into morula and blastocysts, and the intracellular GSH concentrations of blastocyst embryos.

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Flexural Behavior of Segmental U-Girder and Composite U-Girder Using Ultra High Performance Concrete (초고강도 섬유보강 콘크리트를 사용한 분절형 U거더 및 합성 U거더의 휨거동)

  • Lee, Seung-Jae;Makhbal, Tsas-Orgilmaa;Kim, Sung-Tae;Han, Sang-Mook
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.5 no.3
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    • pp.290-297
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    • 2017
  • The flexural behavior tests of UHPC segmental U-girder and composite U-girder which has 160MPa compressive strength and 15.4m length were carried out. The test variables are volume fraction of steel fibers and slab over the U-girder. Each U-girder has longitudinal re-bars in web and lower flange. PS tendons which has 2 of 15.2mm diameter in upper flange and PS tendons which has 7 of 15.2mm diameter in lower flange were arranged and prestressed at onetime in U-girder connection stage. Enough strong prestressing force which applied to U-girder due to ultra high performance concrete strength can withstand the self weight and dead load in U-girder stage. By comparison with the brittle behavior of U-girder, composite U-girder showed the stable and ductile behavior. After the construction of slab over U-girder, flexural load capacity of composite U-girder can bear the design load in final construction stage with only one time prestressing operation which already carried out in U-girder stage. This simple prestressing method due to the ultra high strength concrete have the advantage in construction step and cost. The shear key which has narrow space has the strong composite connection between ultra high strength concrete U-girder and high strength concrete slab didn't show any slip and opening right before failure load.

Vibration Characteristics of Steam Generator U-tubes with Defect (결함을 가진 증기발생기 U-튜브의 진동특성)

  • 조종철;정명조;김웅식;김효정;김태형
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.13 no.5
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    • pp.400-408
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    • 2003
  • This paper investigates the vibration characteristics of steam generator (SG) U-tubes with defect. The operating SG shell-side flow field conditions for determining the fluidelastic instability parameters such as added mass are obtained from three-dimensional SG flow calculation. Modal analyses are performed for the U-tubes either with axial or circumferential flaw with different sizes. Special emphases are on the effects of flaw orientation and size on the modal and instability characteristics of tubes, which are expressed in terms of the natural frequency, corresponding mode shape and stability ratio. Also, addressed is the effect of the internal pressure on the vibration characteristics of the tube.

Production of Nuclease Activity in U937 Cells by Phorbol 12-Myristate 13-Acetate and Lipopolysaccharide

  • Kwon, Hyung-Joo;Kim, Doo-Sik
    • BMB Reports
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    • v.36 no.5
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    • pp.520-523
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    • 2003
  • The proliferation and differentiation signals of myelogeneous U937 cells are provided by extracellular stimuli, such as lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMA). In a DNA-native-polyacrylamide gel assay system, we demonstrated that a particular nuclease activity is expressed in PMA-stimulated U937 cells and secreted into the culture medium. The nuclease activity was induced in U937 cells by LPS treatment, while the secretion of the enzyme was undetected in the culture medium. Therefore, it is likely that the expression and secretion of the particular nuclease in U937 cells are controlled by extracellular stimulations, such as PMA and LPS treatment.

A Case Study on Establishing U-Information HUB System At Incheon International Airport (인천국제공항공사의 U-Information Hub 시스템 구축에 관한 사례연구)

  • Lee, Dong Wook;Suh, Eun Jung
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.5 no.3
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    • pp.201-213
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    • 2009
  • An Airport is important space and even stands for the country because diverse people from other countries use a variety of facilities in the gateway to the country. Therefore, It is urgent for those people to increase efficiency and convenience of its usage. But we have a problem with process of passenger and this paper with the object of finding the solution in case study. This paper focus on information system, especially Intelligent Airport Information System to streamline process of passenger. There are a few of countries adopted this system in process. Therefore, IIAC(Incheon International Airport Corporation) establishing u-Information HUB System. It is unique and differential characteristics to Facilitation and increase passenger's satisfaction. u-Information HUB system unified stakeholder's process of Facilitation in Air Traffic Sides. u-Information HUB system gets the highest state of efficiency to Facilitation. This Paper is attempt to find new effort

Development and Verification of Technical Criteria for a U-Bridge

  • Kim, Kyoon-Tai;Kim, Chang-Han
    • Journal of the Korea Institute of Building Construction
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    • v.10 no.5
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    • pp.145-153
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    • 2010
  • With the recent acceleration in the adoption of IT in industries, converged Construction-IT has been drawing attention as a new market driver in the IT fusion industry. Despite the simultaneous implementation of U-Cities all over Korea in a manner suiting each city's own attributes, Technical Criteria to build an optimized IT-infrastructure have not yet been prepared. To solve this problem, a standard for U-City construction should be prepared. This research, therefore, draw sample contents in order to establish and verify the criteria for U-City construction. The findings are expected to be meaningful, in that they would serve as significant data for establishing the criteria for future U-City construction.

A Study on the Establishment of U-City Data Management (U-City정보의 생산.수집.가공.활용 및 유통 등 정보관리방안 수립 연구)

  • Ahn, Jong-Wook;Shin, Dong-Bin
    • Journal of Korean Society for Geospatial Information Science
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    • v.17 no.2
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    • pp.27-35
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    • 2009
  • U-City law was enacted U-City construction to support efficiently. But U-City planning system is not set. And detailed instructions are not also available. In particular, U-City data is not available and specific plans and instructions. Therefore, in this research, "U-City Law" by the state and local governments need to establish in the U-City Comprehensive Plan and the U-City master plan to be included in U-City data management was presented matters. U-City comprehensive plan provides the direction of the U-City master plan. Detailed action task are (1) U-City data management standards for the establishment of cooperation and sharing (2) Establishment of U-City data processing and activation (3) U-City data distribution system infrastructure (4) Establishment of data quality and price. U-City master plan is based on the U-City comprehensive plan. And U-City master plan for the data managements are (1) U-City data on the production plan (2) U-City data on the collection plan (3) U-City data on the processing plan (4) U-City data on the activation plan (5) U-City data on the distribution plan.

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U-Shaped RFID Tag Antenna with Isotropic Radiation Characteristic (등방성 복사 특성을 가지는 U-형태의 RFID 태그 안테나)

  • Lee, Sang-Woon;Cho, Chi-Hyun;Lee, Kee-Keun;Choo, Ho-Sung;Park, Ik-Mo
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.5
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    • pp.523-532
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    • 2008
  • In this paper, we proposed a U-shaped RFID tag antenna with isotropic radiation characteristic for the stable operation of RFID system. The proposed antenna is composed of a U-shaped half wavelength dipole and a rectangular-shaped feed. In order to have good impedance matching with a tag chip, the commercial tag chip is attached to the lower center of the feed. A gain deviation characteristic of the U-shaped tag antenna can be further improved by inserting a rectangular slit in the lower center of the U-shaped antenna body. On the condition of VSWR<2, the tag antennas of two structures satisfy the Korea UHF RFID bandwidth and showed the gain deviation of less than 1.63 dB and 0.74 dB for without slit and with slit, respectively. On the condition of VSWR<5.8, the U-shaped tag antenna showed the gain deviation of less than 3.8 dB and 1.2 dB for without slit and with slit, respectively.

Analysis on the post-irradiation examination of the HANARO miniplate-1 irradiation test for kijang research reactor

  • Park, Jong Man;Tahk, Young Wook;Jeong, Yong Jin;Lee, Kyu Hong;Kim, Heemoon;Jung, Yang Hong;Yoo, Boung-Ok;Jin, Young Gwan;Seo, Chul Gyo;Yang, Seong Woo;Kim, Hyun Jung;Yim, Jeong Sik;Kim, Yeon Soo;Ye, Bei;Hofman, Gerard L.
    • Nuclear Engineering and Technology
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    • v.49 no.5
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    • pp.1044-1062
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    • 2017
  • The construction project of the Kijang research reactor (KJRR), which is the second research reactor in Korea, has been launched. The KJRR was designed to use, for the first time, U-Mo fuel. Plate-type U-7 wt.% Mo/Al-5 wt.% Si, referred to as U-7Mo/Ale5Si, dispersion fuel with a uranium loading of $8.0gU/cm^3$, was selected to achieve higher fuel efficiency and performance than are possible when using $U_3Si_2/Al$ dispersion fuel. To qualify the U-Mo fuel in terms of plate geometry, the first miniplates [HANARO Miniplate (HAMP-1)], containing U-7Mo/Al-5Si dispersion fuel ($8gU/cm^3$), were fabricated at the Korea Atomic Energy Research Institute and recently irradiated at HANARO. The PIE (Post-irradiation Examination) results of the HAMP-1 irradiation test were analyzed in depth in order to verify the safe in-pile performance of the U-7Mo/Al-5Si dispersion fuel under the KJRR irradiation conditions. Nondestructive analyses included visual inspection, gamma spectrometric mapping, and two-dimensional measurements of the plate thickness and oxide thickness. Destructive PIE work was also carried out, focusing on characterization of the microstructural behavior using optical microscopy and scanning electron microscopy. Electron probe microanalysis was also used to measure the elemental concentrations in the interaction layer formed between the U-Mo kernels and the matrix. A blistering threshold test and a bending test were performed on the irradiated HAMP-1 miniplates that were saved from the destructive tests. Swelling evaluation of the U-Mo fuel was also conducted using two methods: plate thickness measurement and meat thickness measurement.

Reference Spent Nuclear Fuel for Pyroprocessing Facility Design (파이로공정 시설 개념설계를 위한 기준 사용후핵연료 선정)

  • Cho, Dong-Keun;Yoon, Seok-Kyun;Choi, Heui-Joo;Choi, Jong-Won;Ko, Won-Il
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.6 no.3
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    • pp.225-232
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    • 2008
  • An estimation has been made for inventories and characteristics of spent nuclear fuel(SNF) to be generated from existing and planned nuclear power plants based on the 3rd Basic Plan for Electric Power Demand and Supply. The characteristics under consideration in this study are dimensions, a fuel rod array, a weight, $^{235}U$ enrichment, and the discharge burnup in terms of fuel assembly. These are essentially needed for designing a pyroprocessing facility. It is appeared that the anticipated quantity by the end of 2077 is about 23,000 tU for PWR spent nuclear fuel. It is revealed that the proportion of SNF with the initial $^{235}U$ enrichment below 4.5 weight percent(wt.%) is approximately 95 % in total. For SNF with 16$\times$16 fuel rod array the proportion is expected approximately 74% in total. It appears that the average burnup of SNF will be 55 GWd/tU after the medium and/or latter part of 2010s while the average burnup is 45 GWd/tU at present. Finally, a requirement in terms of reference SNF for designing the pyroprocessing facility has been derived from the above-mentioned results. The anticipated SNF seems to be 16$\times$16 Korean Standard Fuel Assembly with a cross section of 21.4 cm$\times$21.4 cm, a length of 453 cm, a mass of 672 kg, the initial $^{235}U$ enrichment of 4.5 wt.%, and the discharge burnup of 55 GWd/tU.

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