Kim, Min-Chul;Lee, Ka-Soon;Lee, Byung-Jin;Kwon, Byung-Gu;Ju, Jung-Il;Gu, Ja-Hyeong;Oh, Man-Jin
Journal of the Korean Society of Food Science and Nutrition
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v.36
no.10
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pp.1307-1313
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2007
In order to evaluate the utilization of immature green wheat kernels as food processing material, these experiments were carried out to analyze general composition, free sugars, free amino acids, mineral content and color of 3 wheat varieties according to their heading dates. Contents such as moisture, crude protein, crude lipid and ash were gradually decreased according to the days after heading of 3 wheat varieties, while crude fiber and starch were gradually increased. Maltose and maltotriose contents in immature green wheat kernels were high but its rapidity decreased by closing to ripeness. Contents of free amino acids showed a tendency to decrease, among which alanine content was the highest around 25 days after heading, and then glutamic acid, GABA, glycine in order. Contents of total free amino acid were decreased by closing to ripeness. Among 3 wheat varieties, its contents of Guru wheat was the highest. As wheat matured, ${\beta}-glucan$ contents were decreased from 1.5% to 0.28% on 43 days after heading. Mineral contents of 3 wheat varieties were generally increased by closing to ripeness after coming into ears, and K, Mg were especially prominent. As wheat matured, lightness was decreased, while redness and yellowness were increased. Considering chemical composition and color, the immature green wheat were produced through blanching the spikes harvested before the yellow ripe stage. The harvested green wheat cereals is able to eat raw wheat or cook it as food processing material.
Nuclear SSR (nSSR) and chloroplast SSR (cpSSR) markers were analyzed to assess the parameters of mating system in seed orchard, such as outcrossing rates, the number of potential pollen contributors, paternal contribution rates, degree of pollen contamination, and biparental inbreeding ($t_m-t_s$). In 2006, 2007, seeds were collected from the seed orchard of Pinus densiflora, established in 1977 at Anmyeon island. Estimates of outcrossing rates ranged from 94.9 to 100% (mean 98.9%) in 2006 and from 91.2 to 100% (mean 97.7%) in 2007 on the basis of the analysis of cpSSR haplotypes and from 90.3 to 100% (mean 95.9%) in 2006 and from 81.6 to 100% (mean 95.3%) in 2007 on the basis of the analysis of nSSR genotypes. By cross checking of both DNA markers, mean cumulative outcrossing rates of 100% and 98.9% were estimated in each year. Mean contamination rates were estimated as 48.9% and 42.4%, respectively. On the basis of cpSSR haplotype observed in each seed, paternal contribution rates (the number of pollen contributors) were estimated as 0.458 (mean 16.2) in 2006 and 0.512 (mean 14.8) in 2007. In conclusion, considering pretty high level of outcrossing rates observed in a seed orchard, there might be little to be influenced by inbreeding depression for genetic potential of the seeds induced by selfing. Estimates of the mating system parameters obtained from the two reproductive years were not statistically different, which revealed stable genetic quality of seeds produced in different years. Observed results from this study may provide useful information for the management and establishment of the seed orchard of the progressive generation.
Kim, Sung Geun;Jung, Hun;Kim, Sin Sun;Jeon, Kyung Hwa;Song, Kyo Young;Kim, Jin Jo;Jin, Hyung Min;Kim, Wook;Park, Cho Hyun;Park, Seung Man;Lim, Keun Woo;Kim, Seung Nam;Jeon, Hae Myung
Journal of Gastric Cancer
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v.7
no.1
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pp.9-15
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2007
Purpose: DNA methylation is an important epigenetic factor in tumorigenesis. We hypothesized that polymorphism of the promoter of the DNA methyltransferase 3b (DNMT3b) genes, which are responsible for regulating the methylation status of tumor suppressor genes, are associated with increased risk of gastric cancer. Materials and Methods: In this hospital-based case-control study, to determine the role of this polymorphism of the promoter of DNA methyltransferase 3b (DNMT3b) genes in gastric cancer, we genotyped 176 cases and 70 control subjects. To determine the genotype, we used a polymerase chain reaction restriction fragment length polymorphism assay. We compared alleles and genotypes between the two groups and revealed an association of DNMT3b promoter polymorphism with increased risk of gastric cancer in the Korean population. Results: Genotype frequencies were 14.8% (Cytosine-Cytosine), 71.6% (Cytosine-Thymine), and 13.6% (Thymine- Thymine) in the case patients and 40.0% (Cytosine-Cytosine), 42.9% (Cytosine-Thymine), and 17.1% (Thymine-Thymine) in the control subjects, respectively. Compared with CC homozygotes, CT heterozygotes had a 4.523-fold increased risk (OR, 2.13; 95% CI, 2.324~8.803), and the TT homozygotes had a 2.154-fold elevated risk (OR, 1.42; 95% CI, 0.899~85.165). For the T variant genotype (CT+TT), there was a 3.846-fold increased risk (OR, 1.88; 95% CI, 2.040~7.251). However, no significance was observed in the genotype distributions of both polymorphisms according to histopathology, stage of stomach cancer. The Ssame results were observed with Helicobacter infection. Conclusion: DNMT3b promoter polymorphism, especially the T variant genotype, is associated significantly with thean increased risk of gastric cancer.
It is judged that celadons with '○' pattern and '⊙' pattern have the indication of manufacturer by engraving mark at the internal center of the bottom. These celadons were produced at No.8.23.27 kiln sites located at Sadang-ri(사당리), Daegu-myon(대구면), Gangjin-gun(강진군), Jeollanamdo(전라남도), Korea and '⊙’ patterned celadon porcelain was found only at No. 23 and 27 kiln sites, so it is assumed that its production sites were limited. Kinds of celadons included bowl, dish, cup, bottle, case and vessel for emptied wine and there were a lot of dishes with a variety of patterns. These celadons were engraved by molded design technique using the same ceramic design as the pattern of celadons in the period of their prosperity and it was appropriate method to produce celadons of a good quality with production method to keep decorative design like pattern or size uniformly. It was also found that inlay method was not universalized in this period and similar celadons were produced with molded design in large quantities. These celadons had partially fire clay temper, but most of them were burnt by supporting the inner bottom of U-type base with quartzite after whole glazing. It was known through relics excavated at Seokreung(석릉) royal tomb of King Heejong(희종) at Ganghwa(강화) and Hyeeumwon(혜음원) temple site at Paju(파주) and military house at Wakamiyaoji(약궁대로) avenue Kamakura(겸창)-city, Japan(일본) that these celadons were manufactured at two quarters of the 13th century centering around 1230s. Therefore, these celadons will be significant materials to fill the gap of the celadons in the 13th century resulted from the record chronologically that green celadons were manufactured in the 12th century. And this period was invaded by Mongolia(몽고), but central control was achieved and green celadons were continuously produced. It was also considered that these celadons were excavated at Gabgot-ri(갑곶리) and Gwanchung-ri(관청리), Ganghwa(강화), Hyeeumwon temple, Paju which played the role of temporary palace, Bu1guksa(불국사) temple at Gyeongju(경주), Silsangsa(실상사) temple at Namwon(남원) and relics of Jangheung(장흥) adjacent to Daeguso(대구소) and these were produced for high class.
This study was conducted to investigate the life history of Compsidia populnea which is major species of the stem-borers on Populus alba{\times}glandulosa$. Peak emergence of the adult of Compsidia populnea L. was around 10th${\sim}$l2nd of May in Chuncheon vicinities, having one generation a year in Korea. There was a preovipositional period of 10.7 days and a ovipositional period of 14.3.days. Mean adult longevity was 11 days for male and 13.8 days for female. Individual eggs were long oval with somewhat narrowed tip at one side and soft, 2.6mm in length, 0.8mm in diameter, and were laid singly under the U-shaped scars which were made by female before oviposition. Total number of scars per female averaged 56.6 ($8{\sim}135$) and eggs were found in 67.9 percent of the total scars. Egg period was $8{\sim}11$ days at $25^{\circ}C$ constant temperature and $7{\sim}14$ days in the field condition. Early young larvae stayed for $2{\sim}3$ weeks under the scars and then boring into the xylem, forming galls at this time. Size of the gall was 1.8cm($1.3{\sim}2.5$) in length, 1.6cm($1.0{\sim}2.2$) in diameter and the length of mine was everage 3.1cm. Fully grown larvae were developed around the early October and overwintered in the mines of galls. Pupation was done around the early of April. Pupal developmental period averaged 11 days($9{\sim}13$ days) at $25^{\circ}C$ constant temperature condition.
Kim, Hee-Cheol;Jung, Sung-Won;Kim, Dae-Kwang;Jung, Chul-Ho
Korean Journal of Biological Psychiatry
/
v.14
no.3
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pp.167-176
/
2007
Objectives:A considerable number of pharmacogenetic studies have been performed in recent years to define the association of antipsychotic drug response with dopamine receptor polymorphisms. The purpose of this study was to investigate the relationship between the therapeutic response to antipsychotic drugs and the polymorphisms of the dopamine D2, D3, and D4 receptor genes(DRD2, DRD3 and DRD4, respectively). Methods:We conducted retrospective chart review of 200 consecutively hospitalized patients with the diagnosis of schizophrenia(DSM-IV) who were treated with various antipsychotics(94% atypical antipsychotics) at Bugok National Hospital, Korea. The patients were divided into two groups, responders and non-responders, by responsiveness to antipsychotic drugs according to a four-point scale used in previous studies; responders included moderate to marked responded patients and non-responders included none to minimal responded patients. We analyzed the Ser311Cys polymorphism in the DRD2, the Ser9Gly polymorphism in the DRD3, and the exon III 48 bp repeat polymorphism in the DRD4. Results:Among the total patients of 200, 141(70.5%) were categorized as responders. There were no significant differences in the frequencies of the DRD2, DRD3, and DRD4 alleles and genotypes between responders and non-responders. Conclusion:These results suggest that the Ser311Cys polymorphism in the DRD2, the Ser9Gly polym- orphism in the DRD3, and the exon III 48bp repeat polymorphism in the DRD4 are not associated with the therapeutic response to antipsychotic drugs in Korean schizophrenic patients. A larger prospective study is needed to elucidate the association between antipsychotic response and dopamine receptor gene polymorphism.
Journal of Korean Tunnelling and Underground Space Association
/
v.19
no.6
/
pp.1059-1075
/
2017
The objective of this study was to evaluate the freezing and thawing resistance of the existing drainage system for leakage treatment of underground concrete structures operating in cold regions. The freezing and thawing test was conducted on 4 types of drainage system specimens to evaluate the freezing and thawing resistance of the drainage system. The freezing and thawing resistance was evaluated on 4 types of Hotty-gel, as a waterproofing material, connection methods and on two methods to fix the drainage board with Hotty-gel on the surface of cement concrete specimen. One cycle of the freeze-thaw testing was 48 hours (24 hours of freezing and 24 hours of thawing), and the temperatures of freezing and thawing were at $-18^{\circ}C$ and $10^{\circ}C$, respectively. Among the 4 types of Hotty-gel connection methods, leakage occurred after 28 cycles (8 weeks) of freeze-thawing only in the Hotty-gel connection method with the 'V' groove applied to the corner of the drainage board. No leakage occurred in the 3 types of Hotty-gel connection methods. In two fixing methods, leakage occurred in the method of fixing the drainage board on the cement concrete specimen using the washer, screw and plastic wall plug. Leakage occurred at one point after 10 cycles (3 weeks) of freezing and thawing. After 28 cycles (8 weeks) of freezing and thawing, leakage point increased to 5 points. As time passed, the leak point was not increased, but the amount of leakage was increased at each leak point. The Hotty-gel connection method with cross-sectional diagonal shape was evaluated to be the highest in the production efficiency considering the production time and manufacturing method of the Hotty-gel connection shape. In the construction efficiency considering the construction time and construction method, the fixing method of air nailer, fixed nail and washer was superior to that of the washer, screw and plastic wall plug.
Kim, Young Mi;Hong, Kyung Nak;Park, Yu Jin;Hong, Yong Pyo;Park, Jae In
Korean Journal of Plant Resources
/
v.28
no.1
/
pp.101-110
/
2015
Parameters of mating system and pollen flow of a Pinus densiflora population in Buan, South Korea, were estimated using seven nuclear microsatellite markers. The expected heterozygosity ($H_e$) was 0.614 in mother trees and 0.624 in seeds. Fixation index (F) was 0.018 and 0.087 in each generation. There was no significant genetic difference between the generations (P > 0.05). From MLTR, the outcrossing rate ($t_m$), the biparental inbreeding ($t_m-t_s$), and the correlation of paternity ($r_p$) were 0.967, 0.057, and 0.012, respectively. tm was larger but $t_m-t_s$ and $r_p$ were smaller than those of allozyme markers in Pinus densiflora. These values were similar to those of microsatellite markers in other pine species. The optimal pollen dispersal model from TwoGener was the normal dispersal model with the effective density of 220 trees/ha and its level of genetic differentiation in pollen pool structure (${\Phi}_{ft}$) was 0.021. The average radial distance of pollen flow (${\delta}$) was calculated as 11.42 m, but no correlation between the pairwise-${\Phi}_{ft}$ and the geographical distance among mother trees was at Mantel test (r = -0.141, P > 0.05). Although the effective pollen dispersal in the population seems to be restricted, the amount of genetic variation might be maintained in each generation without a loss of genetic diversity. It might be because the genetic diversity in pollen pool was high but the genetic difference between pollen donors was small under the complete random mating condition in the Pinus densiflora population in Buan.
This study was carried out to classify vegetation structure of the Hyangjeokbong in the Deogyusan National Park using the gradient analysis and phytosociological method. The vegetation was classified into Quercus mongolica community(Rhododendron schlippenbachii subcommunity, typical subcommunity), Q. serrata community, Q. variabillis community, Pinus densiflora community, Cornus controversa community, Fraxinus mandshurica community and Taxus cuspidata community. Ecological characteristics such as species composition, layer structure, vegetation ratio, and the distribution of individual trees by DBH(diameter at breast height) were significantly different among communities. The order of important value of the forest community with DBH 2cm above plants was Q. mongolica(81.2), F. mandshurica, Q. serrata, P. densiflora, Acer pseudosieboldianum, Q. variabillis, Rhododendron schlippenbachii, C. controversa, T. cuspidata. Distribution of DBH of Q. mongolica and Q. serrata had suggesting a continuous domination of these species over the other species for the time being. In contrast, F. mandshurica appeared limited to the valley of the sheet and a higher frequency of young individuals, suggesting a continuous domination of these species the development of a climax forest terrain. P. densiflora and T. cuspidata had suggesting a continuous domination of these species over the other species for the time being in maintain. Q. variabilis and C. controversa had a formality distribution, suggesting a continuous domination of these species over the other species for the time being. This study examined the correlation between each community and the environment according to DCCA ordination. The Q. mongolica community and T. cuspidata community predominated in the highest elevation habitats which had few moisture, Ca and in the low pH. The Q. serrata community mainly occurred in the low elevation habitats which had many moisture. P. densiflora community predominated in the middle elevation and south-facing slope habitats which had few moisture, Ca and in the low pH. F. mandshurica community predominated in the low elevation habitats which had many moisture, Ca and pH.
Ku, Ja Hyeong;Kim, Tae Ill;Ahn, Joo Won;Lee, Kyu Seung;Kim, Moon Kyu
Korean Journal of Agricultural Science
/
v.19
no.1
/
pp.16-27
/
1992
To determine a potential of new sludge fertilizer for horticultural crops, comparative studies between commercial fertilizers (Jandibiryo and Wonyebokbi) and sludge fertilizer (Sludgebiryo) were made through examining the growth responses on zoysiagrass (Zoysia japponica Steud.) and several horticultural plants. 1. The pH of new sludge fertilizer remained near 6.5 regardless the particle size. The solubility of elements was highest in phosphorus, followed by nitrogen and potassium in the order. Especially, desorption of potassium was continued up to 48 hrs after solubilization. 2. There was an increase in shoot number per plant, length of stolon and rhizome, and root weight as well as clipping yield of zoysiagrass in the treatment of large size Sludgebiryo compared to small one and Jandibiryo. 3. Regardless the size of fertilizers, Sludgebiryo increased flower numbers in salvia (Salvia officinalis L. 'Hatzazz') compared to Wonyebokbi, although the difference was not great, However, leaf area and fresh weight of plant were more increased in Wonyebokbi application. 4. Flower diameter of marigold (Tagetes erecta L. 'Inca') was slightly increased in Sludgebiryo application, but the average number of lateral shoots and fresh weight per plant were significantly increased in the treatment of Wonyebokbi application. 5. Sludgebiryo effectively increased the length of both main and lateral shoots, number of flowers and weight of shoot in vinca (Vinca rosea L. 'Little Linde'), but root growth of plant was higher in Wonyebokbi application. 6, No differences between Wonyebokbi and Sludgebiryo were found in promoting the growth of leaves of perilla (Perilla frutescens Hara 'Yubsil' ), but chlorophyll content and seed weight were slightly higher in the application of Wonyebokbi compared to Sludgebiryo. In conclusion, the effectiveness of Sludgebiryo for horticultural plants was almost equal to commercial fertilizers such as Jandibiryo or Wonyebokbi. Expecially, Sludgebiryo appeared to effective on the growth of zoysiagrass, and the increase of flower size and numbers in flower crops. Results indicate that new-made Sludgebiryo can be recommended for turfgrass culture, and the flower crops in which quality depends on flower number and flower size.
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