• Title/Summary/Keyword: Y/C 분리

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Isolation and Identification of Cellulomonas fimi, Characteristics of its Cellulase and Conversion of the Sawdust into Ethanol (Cellulomonas fimi의 분리(分離) 및 동정(同定), cellulase 특성(特性)과 톱밥의 Ethanol 전환(轉換))

  • Lee, Chan-Yong;Lee, Ke-Ho
    • Applied Biological Chemistry
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    • v.28 no.2
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    • pp.98-105
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    • 1985
  • In the sheep and cattle's rumen, facultative anaerobic cellulolytic bacteria were isolated by using Hungate's roll tube technique. In the 21 isolated species, one was screened by its strong cellulolytic activity and identified as Cellulomonas fimi C-14 by investigate morphological, cultural, physiological characteristics and electron microgram. Optimum conditions of the cell growth and enzyme production were pH 6.5 an $30^{\circ}C$, Thiamine and biotin support a good growth of C. fimi C-14. In the enzyme activities, Crystalline cellulose hydrolyzing activity, CMCase activity and ${\beta}-glucosidase$ activity were 20.6, 226.6 and 0.56$(unit{\times}10^3/ml)$ at pH 6.0, $40^{\circ}C$. By addition of fungal cellulase, enzyme activity was increased. Simultaneous Saccharification Fermentation is better than two step fermentation in ethanol yield with Saccharomyces cerevisiae DY2.

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A Study on the Elution Behavior of Pd-Isonitroso ethylacetoacetate imine Chelates by Reversed-phase High Performance Liquid Chromatography (역상 고성능 액체 크로마토 그래피에 의한 Pd(II) - isonitrosoethylacetoacetate imine 유도체 킬레이트의 용리거동에 관한 연구)

  • Kim, Hyun
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.19 no.1
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    • pp.20-30
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    • 1993
  • Liquid Chromatographic behavior of Pd(II) in Isonitosoethylacetoacetate imine IEAA-NR: R=H, CH3, C2H5, n-C3H7, n-C4H9, C6H5-CH2) Chelates were investigated by reversed phase high performance 1iquid chromatography on Micropak MCH-5 Column using Methanol /water as mobile phase. The optimum condition for the separation of Pd-Isonitrosoethylacetoacetate imine chelates were examined with respect to the flow rate, mobile phase strength. It was found that Pd(IEAA-NR)2 chelates were eluted in an acceptable range of the capacity factor value (0 $\leq$ log k' $\leq$ 1), The dependence of the logarithm of capacity factor(k') on the volume fraction of water in mixture with in the binary mobile phase was examined. Also, the dependence of k'on the liquid-liquid extraction distribution constant in methanol-water / n-alkane extraction system was on system was invert tigated for Pd(IEAA-NR)2. Both kinds of dependence are linear, which suggests that the retention of the electroneutral metal chelates be largely due to the solvophobic effect.

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Studies on the Growth of Excised Root between Indica- Japonica Hybrids and Japonica Type in Paddy Rice (통일형수도와 일본형수도에 있어서 종근.분리근의 생육에 관한 연구)

  • 김진호;서인석;이성춘
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.2
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    • pp.147-154
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    • 1989
  • The experiments were carried out to compare the growth characteristics of excised seminal roots in Japonica type, Indica- Japonica hybrids and its parents. Based on the modified culture medium by Kawata et al., several levels of pH. sucrose and casamino acid were varied in the culture media. The excised root length at 27$^{\circ}C$ incubator was little different between Japonica type and Indica-Japonica hybrids, but at 30$^{\circ}C$ incubator the excised root elongation of Indica-Japonica hybrids was much better than that of Japonica type. The dry matter weight of excised root in Indica- Japonica hybrids was significantly heavier than in Japonica type at both 27 and 30$^{\circ}C$ incubators. The difference of dry root weight between Japonica type and Indica- Japonica hybrids was caused from the number of lateral roots, especially the root numbers of 25 cm length. The main and lateral seminal root growth of Japonica type grew best at the lot of 0.2 percent casamino acid. However, the lots of 0 and 0.2 percent casamino acid showed good results in the main and lateral excised root growth. respectively of Indica-Japonica hydrids. The growth of excised roots was better in Indica-Japonica hybrids than in Japonica type at even various levels of pH, sucrose and casamino acid. Between the parents of Indica - Japonica hybrids, the excised root growth of Indica type was more predominant than that of Japonica type, and the effect of casamino acid on the excised root growth in the formar was similar to that of Indica type. This result indicated that the growth characteristics of excised seminal roots in Indica Japonica hybrids were derived from the parent, Indica type.

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Elucidation of the Mechanism of Propylene/Propane Separation through Faujasite Zeolite Membrane (Faujasite 제올라이트 분리막을 통한 프로필렌/프로판 분리 메카니즘 규명에 대한 연구)

  • Min, Hae-Hyun;Park, You-In;Chang, Jong-San;Park, Yong-Ki;Cho, Churl-Hee
    • Membrane Journal
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    • v.28 no.5
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    • pp.351-360
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    • 2018
  • In this study, propylene/propane separation mechanism through NaY zeolite membrane was investigated. As permeation temperature increased, both propylene and propane permeances increased, saturated and decreased again, and a maximum selectivity was shown at around 50 to $60^{\circ}C$. Propane permeance in mixed gas experiment was much smaller than that in single gas experiment, and propylene/propane mixed gas selectivity was much larger than single gas permselectivity. As permeation time increased in transient permeation experiment, propylene permeance initially increased and saturated, while propane permeance decreased and saturated. All the experimental results announced that propylene/propane separation through NaY zeolite membrane was from preferentially adsorbed propylene molecules. The adsorbed propylene molecules efficiently prevented propane molecules from permeating through the membrane, and sufae diffused through the membrane. NaY zeolite capillary membrane prepared in the present study showed a high mixed gas selectivity of 12 and high propylene permeance of 497 GPU for a propylene/propane (89 : 11) mixture at $50^{\circ}C$ and 4 bar. Therefore, it was concluded that NaY zeolite membrane is one of promising membrane materials for propylene/propane separation due to the low cost and high separation performance.

Isolation and Identification of Mucor mueedo C-7 for Producing The Milk-clotting Enzyme (응유효소를 생산하는 Mucor mucedo C-7의 분리 및 동정)

  • 조재민;이웅수;김교창
    • Microbiology and Biotechnology Letters
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    • v.18 no.5
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    • pp.449-453
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    • 1990
  • This study was attempted to obtain the efficient milk-clotting enzyme from microorganisms as a rennet subtitute. Fungi which showed the formation ability of the milk-clotting enzyme were selected out from samples of soil hay and wastes etc. Among these isolated fungi, strain no. C-7 which had presented higher value in the ratio of milk-clotting activity to proteolytic activity was selected. The hyphae of this strain was white to gray and no septa. A single sporangiophore which stand erectly above growing hyphae was monomucor type without branching. A globose sporangium was developed at the tip of each sporangiophore. The suitable temperature and pH for the growth of no. C-7 was 20-$30^{\circ}C$ and pH 3.0-8.0 respectively. These morphological and physiological characteristics implied that strain no. C-7 was Mueor mucedo.

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The First Isolation of Chalamydia pneumoniae from a Korean Patient (한국인에서 처음 분리된 Chlamydia pneumoniae)

  • Lee, Seung-Joon;Jung, He-Hyeok;Kim, Suk-Kyeong;Choi, Dae-Hee;Han, Seon-Suk;Nam, Eui-Cheol;Won, Jun-Yeon;Park, Weon-Seo;Lee, Myung-Goo;Jung, Ki-Suck
    • Tuberculosis and Respiratory Diseases
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    • v.53 no.5
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    • pp.569-576
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    • 2002
  • Background : Chlamydia pneumoniae is one of common causes in upper and lower respiratory infections. Isolating C. pneumoniae from clinical specimens is very difficult due to the characteristics of the organism. Recently, we succeeded in isolating C. pneumoniae from a Korean patient, who suffered from acute pharyngitis. This is the first isolate from a clinical specimen in Korea. Methods : We attained a nasopharyngeal swab from a 22-year-old female patient, and inoculated it on a monolayer of the Hep-2 cell line. After 8 passages, we found the inclusion bodies of C. pneumoniae by an immunofluorescence(IF) test. The species-specific monoclonal antibody IF staining and species-specific PCR were done to confirm the species of the isolate, and electron microscopy was used to characterize the morphology. Results : The isolated was confirmed to be C. pneumoniae by species-specific IF and PCR, and the strain was named LKK-1. The shape of the elementary body was round and with a narrow periplasmic space, as shown by electron microscopy, which is similar to the Japanese strain, but not the Western strain. Conclusion : We succeeded in isolating C. pneumoniae from a 22-year-old patient with acute pharyngitis, which is the first isolate in Korea. In the future, this Korean strain will be useful to the study of C. pneumoniae.

Isolation and Characterization of the Strain Producing Angiotensin Converting Enzyme Inhibitor from Soy Sauce (간장으로부터 Angiotensin Converting Enzyme 활성 저해물질 생성 균주의 분리 동정)

  • 차명화;박정륭
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.4
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    • pp.594-599
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    • 2001
  • This study was attempted to isolate and identify the strain revealing high angiotensin converting enzyme (ACE) inhibitory activity from various soy fermented foods, i.e. meju, soybean paste and soy sauce. Forty-two strains with morphologically different characteristics were selected and the ACE inhibitory and proteolytic activities were examined. Of the strains tested, SS103 which was isolated from soy sauce showed the highest ACE inhibitory and proteolytic activities and was finally selected for further studies. The SS103 strain showed motility, rod form and ellipsoidal spores. The shape of colonies on the agar media was irregular, mucoidal and surface dull. The strain could grow under aerobic conditions of pH 5~9 and 10~$50^{\circ}C$. Main cellular fatty acid was $C_{15:0}$ anteiso, $C_{17:0}$ cis and $C_{17:0}$ iso, which was 33.9%, 18.8% and 16.5%, respectively. Based upon these morphological, biochemical and cultural properties, SS103 was identified as a Bacillus subtilis. Optimum cultural condition of Bacillus subtilis SS103 was pH 8.0, $37^{\circ}C$ and 48 hr.48 hr.

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클라우드 무선 접속 네트워크 개요와 주요 이슈 분석

  • Park, Sang-Gyu;Lee, Tae-Seop;Park, Se-Ung
    • Information and Communications Magazine
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    • v.30 no.9
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    • pp.78-84
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    • 2013
  • 최근 4세대 이동통신은 기술/경제적인 이유로 클라우드 기지국 (또는 C-RAN: Cloud Radio Access Network) 구조를 택하는 추세이다. C-RAN은 기존 일체형 기지국에서의 MAC/PHY 기능을 담당하는 BBU(Base Band Unit)와 RF신호 송수신 만을 담당하는 RRH(Remote Radio Head)로 분리된 기지국 구조를 가지며, RRH의 무선 송수신 신호는 중앙의 BBU에 집중되어 처리된다. 본고에서는 BBU와 RRH로 분리되어 설치/운용되는 C-RAN의 구조와 기지국 가상화 개념을 소개하며, 향후 이동통신시스템에서의 기술동향과 관련하여 C-RAN에서 발생할 수 있는 이슈들을 살펴본다.

Application of MSAP to JDAM Separation from an F/A-18C Aircraft (MSAP을 이용한 F/A-18C로 부터 JDAM 분리 해석)

  • Lee Seungsoo
    • Journal of computational fluids engineering
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    • v.9 no.1
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    • pp.41-47
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    • 2004
  • A successful store separation analysis tool, MSAP(Multi-body Separation Analysis Program) has been applied to F/A-18C/JDAM CFD Challenge Ⅱ. The challenge was devised to challenge CFD community to use CFD methodologies to predict and match the trajectory of a JDAM MK-84 separating from F/A-l8C. Trajectory simulations for two flight conditions were performed. Comparison between computed and measured flight trajectories for both conditions shows a good agreement.

벼로부터 chloroplast small heat shock protein cDNA의 cloning 및 characterization

  • 이병현;원성혜;이효신;김기용;김미혜;정동민;조진기
    • Proceedings of the Korean Society of Grassland Science Conference
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    • 1999.06a
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    • pp.71.2-72
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    • 1999
  • 고등식물에 있어서 엽록체에 존재하는 저 분자량 heat shock protein (smHSP)은 식물의 내열성 획득에 있어서 필수유전자임이 mutant를 이용한 유전학적인 연구에 의해 보고된 바 있다. 고온내성이 강한 작물인 벼로부터 엽록체 smHSP cDNA를 분리하고자 벼의 잎에서 분리한 mRNA로 작성한 cDNA library로부터 screening하였다. 선발된 smHSP cDNA는 1,026 bp의 염기로 구성되어 있었으며, 239개의 아미노산으로 구성되는 예상분자량 26.6 kDa의 단백질을 code하고 있었다. 또한 다른 식물로부터(중략)

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