• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.29 no.1
• /
• pp.11-14
• /
• 1984

The inheritance of scent in rice leaf and its genetic association with base color, hull color and resistance to bacterial leaf blight were studied in F$_2$ population of two rice crosses, P-33-C-19 (scented)/Zhu-Lian-Ai and P-33-C-19/Kataktara DA2. The scent was identified by leaf analysis at tillering stage. The scented and non-scented plants segregated in the ratio of 1scented: 3non-scented showing that a recessive gene responsible for the expression of scentedness. The scentedness was independently segregated with base color. hull color and resistance to bacterial leaf blight.

• Proceedings of the Membrane Society of Korea Conference
• /
• 1991.04a
• /
• pp.1-4
• /
• 1991

고분자와 희석제 간의 interaction은 phase diagram의 형태로 결정하고 상분리 mechanism은 phase diagram에 의해 설명된다. Thermodynamic interaction이 TIPS 분리막의 구조에 미치는 영향을 조사하기 위하여 PP/C20alkane, PP/C20acid, PP/TA 3가지 system을 선택하였다. 200$^{\circ}$C에서 25 $^{\circ}$C로의 급냉과 분당 10 $^{\circ}$C의 냉각조건을 적용하였다. 각 phase diagram의 형태와 냉각조건에 따라 상분리 mechanism이 달라져서 서론에서 언급한 바와 같이 각기 특유의 구조를 갖게됨을 확인하였다. 같은 종류의 희석제의 chain length가 분리막의 구조에 미치는 영향을 조사하였다. 희석제의 chain length에 따라 결정화 온도가 달라지며 PP와의 결정화 속도 차이에 의하여 구조가 다양하게 변화되었다. 또한 가 희석제의 확산계수의 차이에 의하여 PP 결정화 시 희석제의 외부 방출 정도가 달라 이에 의한 구조의 차이도 관찰되었다. TIPS 공정에서 용액의 조성이 분리막의 구조에 미치는 영향을 조사하였는데, 조성에 의한 nucleation density의 차이에 따라 PP spherulite의 충돌 (impingment)등이 구조에 영향을 미쳤으며 porosity도 변화하였다. 또한 PP/TA system에서는 조성에 따라 상분리 mechanism이 달라져 현격한 구조의 차이를 보였다. 또한 냉각 속도에 따라 PP spherulitic structure가 변화함을 확인하였고, PP 결정화에 따른 희석제의 방출에 의하여 interspherulitic과 intraspherulitic pore를 갖는 2중 구조를 갖게 됨을 알 수 있다.

• Korean Journal of Microbiology
• /
• v.27 no.4
• /
• pp.404-414
• /
• 1989

Attempts were made to isolate and identify Gram-positive or lactic acid bacteria in Kimchi fermentation. Species diversity depended on isolation media and temperatures, and diversity tended to be reduced with decrease of temperature. MRS and KM (natural medium prepared from Kimchi materials) were suitable respectively for isolation and present number of species. Identification of isolates was performed by dichotomous identification schemes arranged on the basis of Bergey's manual of Systematic Bacteriology (1986). Gram-positive bacteria isolated at different temperatures (5, 15, $25^{\circ}C$) were 5 species of Leuconostoc, 4 species of Streptococcus, 3 species of Pediococcus, 2 species of Bacillus and 18 species of Lactobacillus. Species with high frequency of appearance were Lactobacillus plantarum, Streptococcus raffinolactis, Leuconostoc mesenteroides subsp. mesenteroides at $25^{\circ}C$, L. plantarum, Lactobacillus fructosus, L. mesenteroides subsp. mesenteroides at $15^{\circ}C$ and L. mesenteroides subsp. mesenteroides, Leuconosotoc paramesenteroides, Lactobacillus maltaromicus at $15^{\circ}C$. In general, Kimchi fermentation was achieved by Lactobacillus spp. (59.7% frequency) at $25^{\circ}C$ and Leuconostoc spp. (65.2% frequency) at $5^{\circ}C$. Pediococcus cerevisiae and Streptococcus faecalis which have been so far known as bacteria of Kimchi fermentation were not isolated.

• Journal of Life Science
• /
• v.13 no.4
• /
• pp.522-528
• /
• 2003

The RAD4 gene of Saccharomyces cerevisiae is essential for the incision step of UV-induced excision repair. A yeast RAD4 gene has been previously isolated by functional complementation. In order to identify the RAD4 homologous gene from fungus Coprinus cinereus, we have constructed cosmid libraries from electrophoretically separated chromosomes of the C. cinereus. The 13 C. cinereus chromosomes were resolved by pulse-field gel electrophoresis, hybridized with S. cerevisiae RAD4 DNA, and then isolated homologous C. cinereus chromosome. The insert DNA of the RAD4 homolog was contained 3.2 kb. Here, we report the characterization of fungus C. cinereus homolog of yeast RAD4 gene. Southern blot analysis confirmed that C. cinereus contains the RAD4 homolog gene and this gene exists as a single copy in C. cinereus genome. When total RNA isolated from C. cinereus cells was hybridized with the 1.2 kb PvuII DNA fragment of the S. cerevisiae RAD4 gene, a 2.5 kb of transcript was detected. In order to investigation whether the increase of transcripts by DNA damaging agent, transcripts levels were examined after treating the cells. The level of transcript did not increase by untraviolet light (UV). This result indicated that the RAD4 homologous gene is not UV inducible gene. Gene deletion experiments indicate that the RAD4 homologous gene is essential for cell viability.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
• /
• 2004.05a
• /
• pp.397-401
• /
• 2004

장조림 원료 쇠고기 우둔살과 가열 처리한 쇠고기 우둔살에서 중온성 세균, 저온성 세균, 혐기성 세균, 포자형성균 및 대장균군 등과 같은 미생물 균수의 변화를 측정하였고, B. cereus, C. botulinum, C. perfringens, L. monocytogenes 등과 같은 9종류의 주요 병원성 세균의 분리를 시도하였다. 원료육에서 중온균, 저온균, 혐기성 균 등은 대부분 높은 분포를 보였으나 가열 처리육에서는 급격히 감소하였으며, 대장균군, E. coli, Enterpbacteriacea, C. perfringens, S. aureus 등은 원료육과 가열 처리한 쇠고기 우둔살에서 모두 검출되지 않았다. 식중독균인 B. cereus, C. perfringens 및 L. monocytogenes등 3균주가 원료육에서 분리된 반면, C. botulinum, E. coli 0157:H7, Salmonella spp., Shigella spp., S. aureus 및 Y. enterocolitica는 분리되지 않았다.

• Applied Chemistry for Engineering
• /
• v.10 no.3
• /
• pp.445-449
• /
• 1999

Hydrophilic polyphosphazenes were synthesized from hydrophobic polyphosphazenes by adding methoxyethylenoxy side chains and cast by dip-coating method into membranes supported on porous polypropylene mesh filter sheet for metal separation testing. A solution of $Cr^{3+},\;Co^{2+},\;Mn^{2+}$ nitrates was used in diffusion experiments which were conducted from $25^{\circ}C$ to $60^{\circ}C$. lt was found that the ion transport properties were increased as the repeating number of ethylenoxy side chain increased. Membrane from trifluoroethoxy methoxyethoxyethoxyethoxy co-substituted polyphosphazenes was found to separate $Cr^{3+}$ ion from $Mn^{2-}$ and $Co^{2+}$ ions with separation factor of 4.5 at $60^{\circ}C$.

• The Korean Journal of Mycology
• /
• v.38 no.2
• /
• pp.146-151
• /
• 2010

A total of 167,955 microorganisms were isolated from 366,661 clinical specimens. Among them, 6,517 strains of the Candida spp. were isolated from the department of laboratory medicine in "C" hospital from Jan. 1, 2005 to Dec. 31, 2009. All clinical specimens were reviewed by the medical records of patients with Candida by the method of retrospectiveness. From this, we got the some isolated pure cultured yeasts. We identified these yeast by the identification kit system of VITEKII and VITEKII-ID-YST card. The isolation frequencies of Candida spp. were as follows. 56.4%,of C. albicans, 17.7% C tropicalis, 10.7% C glabrata and 9.5% C parapsilosis. The isolated frequency of Candida spp. in 2009 was 1.9 times higher than that in 2005. The clinical materials showing over 10.0% isolation rate were in sputum (30.1%), random urine (25.0%), 15.8% blood (15.8%) and catheterized urine (13.5%) in Candida spp.. The clinical department of showing over 7.0% isolation rate were in pulmonary medicine (20.5%), renal medicine (11.0%), infection disease medicine (10.4%), critical care medicine (10.0%), hematooncology (9.6%), general surgery (7.5%) and gastrointestinal medicine (7.4%) in Candida spp.. In monthly analysis, Candida spp. were most friquency isolated in July (10.6%), but lowest one in February (6.1%). Candida spp. were most frequently isolated in patient of over 50 years old (16.7-40.1%) than those isolated from the patients under the age of 0-49 (1.3-7.5%).

• Journal of Yeungnam Medical Science
• /
• v.4 no.1
• /
• pp.17-23
• /
• 1987

New born rat heart cells were dissociated using trypsin and/or collegenase to elucidate the dissociation efficiency of these two enzymes. And the effect of dimethyl sulfoxide during and immediately after cell dissociation was also investigated to clarify the so-called protective activity of dimethyl sulfoxide on cell performance. The results can be summarized as follows. 1. Cold trypsin 18 hours pretreatment followed by warm collagenase treatment resulted best cell viability and cell yield. 2. Single, warm trypsin treatment gave the poorest result. 3. Dimethyl sulfoxide did not seem to play any protective role during or immediately after rat heart cell dissociation. It had very damaging effect on rat heart cells.

• Korean Journal of Food Science and Technology
• /
• v.31 no.5
• /
• pp.1324-1329
• /
• 1999

This study was carried out to investigate the distribution and characteristics of Listeria monocytogenes isolated from frozen Mandoo and pizza in 1998. A total 72 samples were examined and USDA, FDA and modified cold enrichment methods were used for the detection of Listeria spp. Overall prevalence of L. monocytogenes in frozen foods was 9.7% and L. monocytogenes was isolated from 11.1% of frozen Mandoo and 5.6% of frozen pizza. The highest detection rate of Listeria spp. in frozen Mandoo was found at USDA method and the serotype of L. monocytogenes isolates was 4. Isolated L. monocytogenes was confirmed by PCR method with Hly 1 and 2 as primers. It would be necessary to develop more rapid and specific method to isolate and confirm L. monocytogenes from foods because USDA and PCR methods used in this study took 3-4 days. D value of L. monocytogenes isolate in tryptic soy broth was 49.2 sec at $60^{\circ}C$ and 8.8 sec $at\;65^{\circ}C$, and D value of L. monocytogenes in foods with high distribution rate of Listeria spp. would be necessary to evaluate for the safe use of frozen foods.

• Proceedings of the Korean Operations and Management Science Society Conference
• /
• 1995.09a
• /
• pp.211-219
• /
• 1995

블록-삼각(Block-angular)구조를 갖는 선형 제약식과 분리되지 않는 콘벡스 목적함수의 대규모 비분리 콘벡스 최적화 문제의 해법으로 번들-분해법 (Bundle Based Decomposition)을 이용한 알고리즘 SQA(Separable Quadratic Approximation)은 비분리 콘벡스 프로그램을 분리가능한 2차계획 법(Separable Quadratic Programming) 문제로 근사화시켜 번들-분해법을 축 차적으로 적용한다. 본 연구는 수렴성(local convergence & global convergence) 및 알고리즘 구현 [1]에 이어 이에 대한 수치적용 결과를 중심 으로 소개한다. 수치 적용은 ANSI C로 작성된 SQA 프로그램을 SUN SPARC II에서 실행하였으며 이때 대규모 비분리 최적화 문제의 비분리 목 적함수와 블록-삼각 구조의 선형 제약식들이 계수들은 ANSI C의 랜덤함수 로부터 임의의 값들을 이용하였다. 이와같은 다양한 비분리 콘벡스 최적화 문제에 대한 수렴성, 반복회수 및 처리시간등의 결과와 함께 GAMS/MINOS 의 최적해를 소개한다.