Journal of Yeungnam Medical Science
- Volume 4 Issue 1
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- Pages.17-23
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- 1987
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- 2799-8010(eISSN)
Effects of Trypsin, Collagenase and Dimethyl Sulfoxide on Dissociation of Rat Heart Cells
배양을 위한 심근세포분리에 미치는 Trypsin, Collagenase와 Dimethyl Sulfoxide의 영향
- Park, Chang-Woo (Department of Anatomy, College of Medicine, Yeungnam University) ;
- Lee, Yung-Chang (Department of Anatomy, College of Medicine, Yeungnam University)
- Published : 1987.06.30
Abstract
New born rat heart cells were dissociated using trypsin and/or collegenase to elucidate the dissociation efficiency of these two enzymes. And the effect of dimethyl sulfoxide during and immediately after cell dissociation was also investigated to clarify the so-called protective activity of dimethyl sulfoxide on cell performance. The results can be summarized as follows. 1. Cold trypsin 18 hours pretreatment followed by warm collagenase treatment resulted best cell viability and cell yield. 2. Single, warm trypsin treatment gave the poorest result. 3. Dimethyl sulfoxide did not seem to play any protective role during or immediately after rat heart cell dissociation. It had very damaging effect on rat heart cells.
백서심장조직을 trypsin과 collagenase 두가지의 효소를 사용하여 분리하면서 각 효소의 분리효과와 두가지 효소의 복합적 세포분리효과를 조사하였으며 이와 동시에 dimethyl sulfoxide가 세포분리과정 및 단기간 배양중에 세포에 미치는 효과를 조사한 결과를 다음과 같이 요약할 수 있다. 1. 백서심장조직에서 세포를 분리할 때
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