• Title/Summary/Keyword: X-vector

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Autonomously Mitochondrial Replicating Sequence of Aspergillus nidulans (Aspergillus nidulans mtDNA의 자가복제절편)

  • 장승환;한동민;장광엽
    • Korean Journal of Microbiology
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    • v.35 no.3
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    • pp.218-225
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    • 1999
  • We isolated the ANRI fragment from Aspergillus nidulons that could autononlously replicate and enhance transformation efficiency about $10^4$ fold compared lo the integrative vector in Saccha,omgcer cerevisioe. In A. nidulans recombinant plasmid pLJ16-4.5 which carries the 4.5 kb EcoRI fragment of ANRI showed a 170-[old increase of transformation efficiency compared to the integrative vector pLJ16 and could be recovered from iransfonnants as an intact form. Estimated copy number of transforming plasmid pLJ16-4.5 was scored as 2 to 3 copies in transformed A. nidulans. Recoinbinant plasinid pILJ16-4.5 is inilotically unstable; being lost Irom 65% of aswual progeny of transformants on selective medium and 90% on complete medium. Southern analysis of transformant DNA showed that the pILJ16-4.5 is maintained in free form. The sequencing data showed that ANRl fragment was originated from mitochondiral DNA of A. nid~ilans and contained high AT content as much as 74.7%. One ARS consensus sequence (A/T)TTr4T(A/G)TTT(AiT). I I ARS-like sequence (agreement 10 of 11) and ABFl binding core consensus sequence (TCN7ACG). Also six gyrase binding core consensus sequence (YRTGNYNNY: y=C or T, R=A or G, N=A, G, C or T) of $\Phi$X174 and SV40 DNA and one b site (CACTTTACC) combining with gyrase in ColEl are shown. ANRl can be developed as a repl&ng plasinid for lransfoimation system in A. nirlulmis.

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A Fast 4X4 Intra Prediction Method using Motion Vector Information and Statistical Mode Correlation between 16X16 and 4X4 Intra Prediction In H.264|MPEG-4 AVC (H.264|MPEG-4 AVC 비디오 부호화에서 움직임 벡터 정보와 16~16 및 4X4 화면 내 예측 최종 모드간 통계적 연관성을 이용한 화면 간 프레임에서의 4X4 화면 내 예측 고속화 방법)

  • Na, Tae-Young;Jung, Yun-Sik;Kim, Mun-Churl;Hahm, Sang-Jin;Park, Chang-Seob;Park, Keun-Soo
    • Journal of Broadcast Engineering
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    • v.13 no.2
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    • pp.200-213
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    • 2008
  • H.264| MPEG-4 AVC is a new video codingstandard defined by JVT (Joint Video Team) which consists of ITU-T and ISO/IEC. Many techniques are adopted fur the compression efficiency: Especially, an intra prediction in an inter frame is one example but it leads to excessive amount of encoding time due to the decision of a candidate mode and a RDcost calculation. For this reason, a fast determination of the best intra prediction mode is the main issue for saving the encoding time. In this paper, by using the result of statistical relation between intra $16{\times}16$ and $4{\times}4$ intra predictions, the number of candidate modes for $4{\times}4$ intra prediction is reduced. Firstly, utilizing motion vector obtained after inter prediction, prediction of a block mode for each macroblock is made. If an intra prediction is needed, the correlation table between $16{\times}16$ and $4{\times}4$ intra predicted modes is created using the probability during each I frame-coding process. Secondly, using this result, the candidate modes for a $4{\times}4$ intra prediction that reaches a predefined specific probability value are only considered in the same GOP For the experiments, JM11.0, the reference software of H.264|MPEG-4 AVC is used and the experimental results show that the encoding time could be reduced by 51.24% in maximum with negligible amounts of PSNR drop and bitrate increase.

Construction of a Plant Chloroplast Transformation Vector to Produce the Antimicrobial Peptide Stomoxyn (대장균에서 항균 펩타이드 stomoxyn을 생산하기 위한 형질전환 벡터 제작)

  • Jin Gyu Go;Hyeon Ho Gil;Soon Young Kim
    • Journal of Life Science
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    • v.34 no.7
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    • pp.493-499
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    • 2024
  • Antimicrobial peptides are antimicrobial substances inherent in animals and plants, with strong antibacterial activity even in small amounts and with various other functions such as antiviral and antioxidant actions. Plants can be grown with just water and sunlight, allowing for their mass production at low costs. However, transforming a chloroplast into one that produces antimicrobial peptides, rather than growing plants, increases the amount of protein expression and minimizes contamination of the ecosystem because gene transfer by pollen does not occur. In that context, using transgenic plant chloroplasts to produce recombinant proteins increases protein degradation and reduces the solubility of proteins. To solve this problem, we fused SUMO, a fusion protein, with a recombinant protein. We also used a 6xHis tag to purify the fusion protein. The antimicrobial peptide stomoxyn is an antibacterial substance found in stable flies. Stomoxyn has an α-helix structure and is amphiphilic, which allows it to dissolve bacterial cell membranes. In this study, we constructed a transformation vector to express stomoxyn in both plant chloroplasts and Escherichia coli and used this vector to confirm the expression of stomoxyn in E. coli. The expression of the protein was then confirmed in E. coli using a transformation vector. The expressed stomoxyn was purified by nickel column and SUMOase treatment, and its antibacterial activity was confirmed using an agar diffusion assay. The EGFP gene was used to ensure that the transformed vector was inserted into the chloroplast.

Fast Game Encoder Based on Scene Descriptor for Gaming-on-Demand Service (주문형 게임 서비스를 위한 장면 기술자 기반 고속 게임 부호화기)

  • Jeon, Chan-Woong;Jo, Hyun-Ho;Sim, Dong-Gyu
    • Journal of Korea Multimedia Society
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    • v.14 no.7
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    • pp.849-857
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    • 2011
  • Gaming on demand(GOD) makes people enjoy games by encoding and transmitting game screen at a server side, and decoding the video at a client side. In this paper, we propose a fast game video encoder for multiple users over network with low-powered devices. In the proposed system, the computational complexity of game encoders is reduced by using scene descriptors, which consists of an object motion vector, global motion, and scene change. With additional information from game engines, the proposed encoder does not need to perform various complexity processes such as motion estimation and ratedistortion optimization. The motion estimation and rate-distortion optimization skipped by scene descriptors. We found that the proposed method improved 192 % in terms of FPS, compared with x264 software. With partial assembly code, we also improved coding speed by 86 % in terms of FPS. We found that the proposed fast encoder could encode over 60 FPS for real-time GOD applications.

Synthesis of Uniform CA and 90/150 Hybrid CA (Uniform CA와 90/150 Hybrid CA의 합성)

  • Kim, Han-Doo;Cho, Sung-Jin;Choi, Un-Sook;Kwon, Min-Jeong;Kong, Gil-Tak
    • The Journal of the Korea institute of electronic communication sciences
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    • v.11 no.3
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    • pp.293-302
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    • 2016
  • In this paper we analyze the CA formed by combining the uniform 102 CA $\mathbb{C}_u$ and the m-cell 90/150 hybrid CA $\mathbb{C}_h$ whose characteristic polynomial is $(x+1)^m$. We analyze cycle structures of complemented group CA derived from $\mathbb{C}_u$ and propose a condition of complemented CA dividing the entire state space into smaller cycles of equal lengths. And we analyze the cycle structure of complemented group CA $\mathbb{C}^{\prime}$ derived from the CA $\mathbb{C}$ formed by combining $\mathbb{C}_u$ and $\mathbb{C}_h$ with complement vector F such that $(T+I)^{q-1}F{\neq}0$ where $(x+1)^q$ is the minimal polynomial of $\mathbb{C}$.

Design and Implementation of LTE-TDD 2×2 MIMO Bidirectional RF Hybrid Beamforming System (LTE-TDD 2×2 MIMO 양방향 RF 하이브리드 빔포밍 시스템 설계 및 구현)

  • Lee, Kwang-Suk;Kim, Dong-Hyun;Oh, Hyuk-Jun
    • Journal of Korea Society of Industrial Information Systems
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    • v.23 no.4
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    • pp.23-31
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    • 2018
  • This paper presented the implementation and design of the 2T-2R wireless HD video streaming systems over 1.7 GHz frequency band using 3GPP LTE-TDD standard on NI USRP RIO SDR platform. The baseband of the system used USRP RIO that are stored in Xilinx Kintex-7 chip to implement LTE-TDD transceiver modem, the signal that are transmitted from USRP RIO up or down converts to 1.7 GHz by using self-designed 1.7 GHz RF transceiver modules and it is finally communicated HD video data through self-designed 2x9 sub array antennas. It is that communication method between USRP RIO and Host PC use PCI express x4 to minimize delay of data to transmit and receive. The implemented system show high error vector magnitude performance above 32 dBc and to transmit and receive HD video in experiment environment anywhere. The proposed hybrid beam forming system could be used not only in the future 5G mobile communication systems under 6 GHz frequency band but also in the systems over 6 GHz frequency band like ones in mmWave frequency bands.

Transceiver IC for CMOS 65nm 1-channel Beamformer of X/Ku band (X/Ku 대역 CMOS 65nm 단일 채널 빔포머 송수신기 IC )

  • Jaejin Kim;Yunghun Kim;Sanghun Lee;Byeong-Cheol Park;Seongjin Mun
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.24 no.4
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    • pp.43-47
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    • 2024
  • This paper introduces a phased-array single-channel transceiver beamformer IC built using 65nm CMOS technology, covering the 8-16 GHz range and targeting the X and Ku bands for radar and satellite communications. Each signal path in the IC features a low noise amplifier (LNA), power amplifier (PA), phase shifter (PS), and variable gain amplifier (VGA), which allow for phase and gain adjustments essential for beam steering and tapering control in typical beamforming systems. Test results show that the phase-compensated VGA offers a gain range of 15 dB with 0.25 dB increments and an RMS gain error of 0.27 dB. The active vector modulator phase shifter delivers a 360° phase range with 2.8125° steps and an RMS phase error of 3.5°.

A FUNCTIONAL CENTRAL LIMIT THEOREM FOR MULTIVARIATE LINEAR PROCESS WITH POSITIVELY DEPENDENT RANDOM VECTORS

  • KO, MI-HWA;KIM, TAE-SUNG;KIM, HYUN-CHULL
    • Honam Mathematical Journal
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    • v.27 no.2
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    • pp.301-315
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    • 2005
  • Let $\{A_u,\;u=0,\;1,\;2,\;{\cdots}\}$ be a sequence of coefficient matrices such that ${\sum}_{u=0}^{\infty}{\parallel}A_u{\parallel}<{\infty}$ and ${\sum}_{u=0}^{\infty}\;A_u{\neq}O_{m{\times}m}$, where for any $m{\times}m(m{\geq}1)$, matrix $A=(a_{ij})$, ${\parallel}A{\parallel}={\sum}_{i=1}^m{\sum}_{j=1}^m{\mid}a_{ij}{\mid}$ and $O_{m{\times}m}$ denotes the $m{\times}m$ zero matrix. In this paper, a functional central limit theorem is derived for a stationary m-dimensional linear process ${\mathbb{X}}_t$ of the form ${\mathbb{X}_t}={\sum}_{u=0}^{\infty}A_u{\mathbb{Z}_{t-u}}$, where $\{\mathbb{Z}_t,\;t=0,\;{\pm}1,\;{\pm}2,\;{\cdots}\}$ is a stationary sequence of linearly positive quadrant dependent m-dimensional random vectors with $E({\mathbb{Z}_t})={{\mathbb{O}}$ and $E{\parallel}{\mathbb{Z}_t}{\parallel}^2<{\infty}$.

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Morningside Pi2 Pulsation Observed in Space and on the Ground

  • Ghamry, Essam
    • Journal of Astronomy and Space Sciences
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    • v.32 no.4
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    • pp.305-310
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    • 2015
  • In this study, we examined a morningside Pi2 pulsation, with a non-substorm signature, that occurred in very quiet geomagnetic conditions (Kp = 0) at 05:38 UT on December 8, 2012, using data obtained by Van Allen Probes A and B (VAP-A and VAP-B, respectively) and at a ground station. Using 1 sec resolution vector magnetic field data, we measured the X-component of the pulsation from the Abu Simbel ground station (L = 1.07, LT = UT +2 hr, where LT represents local time) in Egypt. At the time of the Pi2 event, Abu Simbel and VAP-A (L = 3.3) were in the morning sector (07:38 LT and 07:59 MLT, respectively, where MLT represents magnetic local time), and VAP-B was in the postmidnight sector (04:18 MLT and L = 5.7). VAP-A and VAP-B observed oscillations in the compressional magnetic field component (Bz), which were in close agreement with the X-component measurements of the Pi2 pulsation that were made at Abu Simbel. The oscillations observed by the satellites and on the ground were in phase. Thus, we concluded that the observed morningside Pi2 pulsation was caused by the cavity resonance mode rather than by ionospheric current systems.

THE SOLAR-B MISSION

  • ICHIMOTO KIYOSHI;TEAM THE SOLAR-B
    • Journal of The Korean Astronomical Society
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    • v.38 no.2
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    • pp.307-310
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    • 2005
  • The Solar-B is the third Japanese spacecraft dedicated for solar physics to be launched in summer of 2006. The spacecraft carries a coordinated set of optical, EUV and X-ray instruments that will allow a systematic study of the interaction between the Sun's magnetic field and its high temperature, ionized atmosphere. The Solar Optical Telescope (SOT) consists of a 50cm aperture diffraction limited Gregorian telescope and a focal plane package, and provides quantitative measurements of full vector magnetic fields at the photosphere with spatial resolution of 0.2-0.3 arcsec in a condition free from terrestrial atmospheric seeing. The X-ray telescope (XRT) images the high temperature (0.5 to 10 MK) corona with improved spatial resolution of approximately 1 arcsec. The Extreme Ultraviolet Imaging Spectrometer (EIS) aims to determine velocity fields and other plasma parameters in the corona and the transition region. The Solar-B telescopes, as a whole, will enable us to explore the origins of the outer solar atmosphere, the corona, and the coupling between the fine magnetic structure at the photosphere and the dynamic processes occurring in the corona. The mission instruments (SOT/EIS/XRT) are joint effort of Japan (JAXA/NAO), the United States (NASA), and the United Kingdom (PPARC). An overview of the spacecraft and its mission instruments are presented.