• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.39 no.11
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• pp.995-1004
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• 2011

Object X-ray method commonly used for hole search in overset grids requires huge amount of time due to complicated vector calculations to search the cross-points as well as time-consuming hole search algorithm with respect to background grids. Especially, when the grid system is in motion relative to the background, hole points should be searched at every time step, leading to hung computational burden. To cope with this difficulties, this study presents an efficient hole search algorithm mainly designed to reduce hole searching time. To this end, virtual surface with reduced grid points is suggested and logical operators are employed as a classification algorithm instead of complicated vector calculations. In addition, the searching process is further accelerated by designating hole points in a row rather than discriminating hole points with respect to each background grid points. If there exists a relative motion, the present algorithm requires much less time because only the virtual surface needs to be moved at every time step. The hole searching time has been systematically compared for a few selected geometries.

• Journal of Life Science
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• v.15 no.6 s.73
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• pp.928-934
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• 2005

ATF2 is a cellular transcription factor which belongs to the CREB/ATF class and it is leucine zipper protein which generally binds to DNA as dimers. This paper presents the procedure for subcloning the ATF2 gene and the results of experiment used the expressed ATF2. The pET expression vector was used since it produced 6xHis fusion protein for easy purification using affinity column. The Nickel chelating chromatography was used for Purifying the expressed ATE2 from E- codi BL2l. Subsequen시y In vitro binding pull-down assay showed the binding specificity of ATF2 with AP-1 family factors such as BATF, c-Fos, c-Jun and ATF2 itselgf. ATF2 forms homodimer as well as strong heterodimer with BATF. It also forms stable dimer with c-Jun but barely binds with c-Fos.

• BMB Reports
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• v.40 no.5
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• pp.656-661
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• 2007

In this report, we describe an optimized method for generation of ephA8 BAC transgenic mice expressing the lacZ reporter gene under ephA8 regulatory sequences. First, we constructed a targeting vector that carries a 1.2 kb ephA8 DNA upstream of its first exon, a lacZ expression cassette, a kanamycin cassette, and a 0.7 kb ephA8 DNA downstream of its first exon. Second, the targeting vector was electroporated into cells containing the ephA8 BAC and pKOBEGA, in which recombinases induce a homologous recombination between the ephA8 BAC DNA and the targeting vector. Third, the FLP plasmid expressing the Flipase was electroporated into these bacteria to eliminate a kanamycin cassette from the recombinant BAC DNA. The appropriate structures of the modified ephA8 BAC DNA were confirmed by Southern analysis. Finally, BAC transgenic mouse embryos were generated by pronuclear injection of the recombinant BAC DNA. Whole mount X-gal staining revealed that the lacZ reporter expression is restricted to the anterior region of the developing midbrain in each transgenic embryo. These results indicate that the ephA8 BAC DNA contains most, if not all, regulatory sequences to direct temporal and spatial expression of the lacZ gene in vivo.

• Journal of Life Science
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• v.11 no.4
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• pp.385-391
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• 2001

Filamentous hemagglutinin (FHA) is considered as an essential immunogenic component for incorporation into acellular vaccines against Bordetella pertussis, the causative agent of whooping cough. Classically, antipertussis vaccination has employed an intramuscular route. An alternative approach to stimulate mucosal and systemic immune responses is oral immunization with recombinant live vaccine carrier strains of Salmonella typhimurium. An attenuated live Salmonella vaccine sgrain($\Delta$cya $\Delta$crp) expressing recombinant FHA(rFHA) was developed. Stable expressionof rFHA was achieved by the use of balanced-lethal vector-host system. which employs an asd deletion in the host chromosome to impose in obligate requirement for diaminopimelic acid. The chromosomal $\Delta$asd mutation was complemented by a plasmid vector possessing the asd$^{+}$ gene. A 3 kb DNA fragment encoding immuno dominant regionof FHA was subcloned in-frame downstream to the ATG translation initiation codon in the multicopy Asd$^{+}$ pYA3341 vector to create pYA3457. Salmonella vaccine harboring pYA3457 expressed approximately 105kDa rFHA protein. The 100% maintenance of [YA3457 in vaccine strain was confirmed by stability examinations. Additionally, a recombinant plasmid pYA3458 was constructed to overpress His(8X)-tagged rFHA in Essherichia coli. His-tagged rFHA was purified from the E. coli strain harboring pYA3458 using Ni$^{2+}$-NTA affinity purification system.>$^{2+}$-NTA affinity purification system.

• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.193-199
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• 2010

We report here the production of transgenic chickens that can regulate human erythropoietin (hEPO) gene expression. The glycoprotein hormone hEPO is an essential for viability and growth of the erythrocytic progenitors. Retrovirus vector system used in this study has two features including tetracycline-controllable promoter and woodchuck hepatitis virus posttranscriptional regulator element (WPRE). The former is for to reduce the possibility of physiological disturbance due to constitutional and unregulated expression of hEPO gene in the transgenic chicken. The latter is for maximum expression of the foreign gene when we turn-on the gene expression. A replication-defective Moloney murine leukemia virus (MoMLV)-based vectors packaged with vesicular stomatitis virus G glycoprotein (VSV-G) was injected beneath the blastoderm of non-incubated chicken embryos (stage X). Out of 325 injected eggs, 28 chicks hatched after 21 days of incubation and 16 hatched chicks were found to express the hEPO gene delivered by the vector. The biological activity of the recombinant hEPO in transgenic chicken serum was comparable to its commercially available counterpart. The recombinant hEPO in transgenic chicken serum had N- and O-linked carbohydrate simillar to that produced from in vitro cultured cells transformed with hEPO gene.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.4
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• pp.1471-1476
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• 2012

Background and Aim: Currently available systemic therapies for malignant melanoma produce low response rates in patients, and more effective treatment modalities are clearly needed. The tumor necrosis factor (TNF)-related apoptosis-inducing ligand has a significant impact on therapy for patients with X-linked inhibitor of apoptosis protein-downregulation malignant melanoma. The primary objective of this study was to assess its therapeutic potential. Materials and Methods: We employed a conditionally replicating oncolytic adenoviral vector, named CRAd5.TRAIL/siXIAP, with the characteristics of over-expression of the therapeutic gene TRAIL and downregulation of XIAP in one vector. B16F10-luc cells were employed to detect anti-tumor activity of CRAd5.TRAIL/siXIAP in vitro and in vivo. Results: CRAd5.TRAIL/siXIAP enhanced caspase-8 activation and caspase-3 maturation in B16F10 cells in vitro. Furthermore, it more effectively infected and killed melanoma cells in vitro and in vivo than other adenoviruses. Conclusion: Taken together, the combination of upregulation of TRAIL and downregulation of siXIAP with one oncolytic adenoviral vector holds promise for development of an effective therapy for melanomas and other common cancers.

• Computers and Concrete
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• v.20 no.5
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• pp.555-562
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• 2017

The accuracy and integrity of stress data acquired by bridge heath monitoring system is of significant importance for bridge safety assessment. However, the missing and abnormal data are inevitably existed in a realistic monitoring system. This paper presents a data reconstruction approach for bridge heath monitoring based on the wavelet multi-resolution analysis and support vector machine (SVM). The proposed method has been applied for data imputation based on the recorded data by the structural health monitoring (SHM) system instrumented on a prestressed concrete cable-stayed bridge. The effectiveness and accuracy of the proposed wavelet-based SVM prediction method is examined by comparing with the traditional autoregression moving average (ARMA) method and SVM prediction method without wavelet multi-resolution analysis in accordance with the prediction errors. The data reconstruction analysis based on 5-day and 1-day continuous stress history data with obvious preternatural signals is performed to examine the effect of sample size on the accuracy of data reconstruction. The results indicate that the proposed data reconstruction approach based on wavelet multi-resolution analysis and SVM is an effective tool for missing data imputation or preternatural signal replacement, which can serve as a solid foundation for the purpose of accurately evaluating the safety of bridge structures.

• Journal of Information Processing Systems
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• v.12 no.3
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• pp.436-454
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• 2016

This research presents the battery discharge rate models for the energy consumption of mobile phone batteries based on machine learning by taking into account three usage patterns of the phone: the standby state, video playing, and web browsing. We present the experimental design methodology for collecting data, preprocessing, model construction, and parameter selections. The data is collected based on the HTC One X hardware platform. We considered various setting factors, such as Bluetooth, brightness, 3G, GPS, Wi-Fi, and Sync. The battery levels for each possible state vector were measured, and then we constructed the battery prediction model using different regression functions based on the collected data. The accuracy of the constructed models using the multi-layer perceptron (MLP) and the support vector machine (SVM) were compared using varying kernel functions. Various parameters for MLP and SVM were considered. The measurement of prediction efficiency was done by the mean absolute error (MAE) and the root mean squared error (RMSE). The experiments showed that the MLP with linear regression performs well overall, while the SVM with the polynomial kernel function based on the linear regression gives a low MAE and RMSE. As a result, we were able to demonstrate how to apply the derived model to predict the remaining battery charge.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.1
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• pp.163-168
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• 2011

Blogs are an easy way to publish information, engage in discussions, and form communities on the Internet. Recently, there are several varieties of spam blog whose purpose is to host ads or raise the PageRank of target sites. Our purpose is to develope the system which detects these spam blogs (splogs) automatically among blogs on Web environment. After removing HTML of blogs, they are tagged by part of speech(POS) tagger. Words and their POS tags information is used as a feature type. Among features, we select useful features with X2 statistics and train the SVM with the selected features. Our system acquired 90.5% of F1 measure with SPLOG data set.

• Journal of information and communication convergence engineering
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• v.8 no.4
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• pp.349-354
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• 2010

Moving pixel displacement detection algorithm using correlation functions for making panorama image on the continuous images is presented in this paper. The input images get from a CMOS image sensor (CIS). The camera is maintained by constant brightness and uniform sensing area in test input pattern. For simple navigation and capture image has to 70% overlapped region. A correlation rate in two image data is evaluated by using reference image with first captures, and compare image with next captures. The displacement of the two images are expressed to second order function of x, y and solved with finding the coefficient in second order function. That results in the change in the peak correlation displacement from the reference to the compare image, is moving to pixel length. The navigating error is reduced by varying the path because the error is shown in the difference of the positioning vector between the true pixel position and the navigated pixel position. The algorithm performance is evaluated to be different from the error vector to vary the navigating path grid.