• Biomedical Science Letters
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• v.12 no.3
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• pp.139-143
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• 2006

Jopock (Jpk) has previously been ascertained that induces both bacterial and mammalian cell death. The Escherichia coli cells expressing Glutathion S-transferase (GST) fused Jpk showed elongated phenotype and inhibited cell growth which led eventual cell death. In an attempt to search the genetic suppressor of the lethal protein Jpk in bacterial cells, we constructed a unidirectional protein expression vector inserting tac promoter next to the C-terminus Jpk in pGEX-Jpk. The function of additional tac promoter was confirmed by substituting lac promoter in Plac-TOPO plasmid. The cells harboring plac- TOPO, which regulates $lacZ{\alpha}$ gene expression under lac promoter, formed blue colonies in 5-bromo-4-3 $indolyo-{\beta}-D-galactoside$ (X-gal) plate. When lac promoter was changed to tac promoter, same results were observed. Since the addition of tac promoter did not affect the toxic effect of Jpk, the pGEX-Jpk-ptac could be a useful vector for the screening of suppressor(s) for Jpk, in which GST-Jpk and a putative Jpk-suppressing protein are coexpressing from two unidirectional tac promoters, which response to the same inducer, $isopropyl-{\beta}-D-thiogalactopyranoside (IPTG)$.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.95-96
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• 2003

본 연구에서는 VSV-G(vesicular stomatitis virus G glycoprotein)로 포장된 MoMLV(Moloney murine leukemia virus) retrovirus vector system을 이용하여 GFP가 발현되는 형질전환 닭을 생산하고자 하였다. GFP 유전자를 retroviral vector 내의 RSV(Rous sarcoma virus) promoter의 조절 하에 도입한 후, Gp293 세포주에서 virus 형태로 생산하였으며, 이 virus를 초원심분리로 고농축하여 stage X 계란의 배 반엽 층에 주입하여 GFP가 발현되는 형질전환 닭을 생산하였다. 생산된 닭에서의 GFP의 발현은 epifluorescence stereomicroscope를 이용하여 확인하였다. 이 방법은 기존의 여러 형질전환 가금 방법에 비하여 기술적인 용이성과 경제성을 가지므로(Muramatsu, Park and Okumura 1998), 매우 효율적이고 주목할 만한 형질전환 가금 생산 방법으로 사료된다. 형질전환 가금의 생산에서 retrovirus vector를 이용하는 방법은 다양한 종류의 표적세포에 대하여 retrovirus 고유의 감염성에 의한 외래 유전자의 전이가 용이하고, 전이된 유전자가 진정염색질 영역 내로 선택적으로 도입될 수 있으며 유전적으로 안정성을 나타내므로 매우 효과적인 방법이다. 그러나 가금에서는 초기 배 발달에 의한 급격한 세포의 수적 증가로 인해 고감염성 virus의 획득이 요구되므로, 본 연구에서는 virus의 농축에 있어 보다 안정적이고 숙주 범위에 있어서 pantropic한 VSV-G에 기반을 둔 retrovirus vector system을 확립하였다. 이 system은 기존의 형질전환 닭의 생산방법에 비해 외래 유전자의 전이에 있어서 매우 효과적인 것으로 확인되었으며, 또한 여러 유용한 생리활성물질을 분비하는 형질전환 동물의 생산에 있어서 상당한 기여를 할 것으로 사료된다.

• Korean Journal of Animal Reproduction
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• v.26 no.3
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• pp.205-214
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• 2002

There are many methods to introduce exogenous DNA into embryo to produce transgenic animals. Exogenous gene can be integrated into oocyte by sperm vector. In this study, sperm was used as a vector for a transgene, which is encoding enhanced green fluorescent protein (EGFP). The objective of this study was to investigate the expression of exogenous gene in bovine embryos after injection of spermatozoa cocultured with EGFP DNA fragment. Spermatozoa were plunged into liquid nitrogen and thawed several times or shook in 0.2% Triton X-100 to remove sperm membrane followed by DTT treatment. The injected oocytes were co-cultured with vero cells in CR1aa, and expression of EGFP gene was observed under fluorescent microscope. Blastocyst formation rates of oocytes injected with sperm treated with DTT, DTT-freezing or DTT-Triton X-100 were 34.7, 39.4 and 31.9%, respectively. The rates of EGFP expression in oocytes injected with 54 ng DNA after DTT-treated, DTT-freezing and DTT-Triton X-100-treated sperm were 0, 19.1 and 13.9%. On the other hands, expression rate of oocytes injected with sperm cocultured with 13.5, 27 and 63.5 ng of EFGP DNA were 6.7, 9.0 and 5.1%, respectively. When intact sperm was mixed with 63.5 ng/${mu}ell$ EGFP DNA fragment, and then electroporated before injection, the expression rate of injected oocyte was 2%. Unexpectedly, electro-poration could not increase the expression rate. These results suggest that sperm can be used as a transgene vector, even if the efficiency was low (19.1%).

• Korean Journal of Mathematics
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• v.30 no.4
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• pp.593-601
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• 2022

Let C(Q) denote Yeh-Wiener space, the space of all real-valued continuous functions x(s, t) on Q ≡ [0, S] × [0, T] with x(s, 0) = x(0, t) = 0 for every (s, t) ∈ Q. For each partition τ = τ_m,n = {(s_i, t_j)|i = 1, . . . , m, j = 1, . . . , n} of Q with 0 = s₀ < s₁ < . . . < s_m = S and 0 = t₀ < t₁ < . . . < t_n = T, define a random vector X_τ : C(Q) → ℝ^mn by X_τ (x) = (x(s₁, t₁), . . . , x(s_m, t_n)). In this paper we study the conditional integral transform and the conditional convolution product for a class of cylinder type functionals defined on K(Q) with a given conditioning function X_τ above, where K(Q)is the space of all complex valued continuous functions of two variables on Q which satify x(s, 0) = x(0, t) = 0 for every (s, t) ∈ Q. In particular we derive a useful equation which allows to calculate the conditional integral transform of the conditional convolution product without ever actually calculating convolution product or conditional convolution product.

• The KIPS Transactions:PartA
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• v.16A no.6
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• pp.411-418
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• 2009

Vector quantization algorithm based on fuzzy clustering has been widely used in the field of data compression since the use of fuzzy clustering analysis in the early stages of a vector quantization process can make this process less sensitive to its initialization. However, the process of fuzzy clustering is computationally very intensive because of its complex framework for the quantitative formulation of the uncertainty involved in the training vector space. To overcome the computational burden of the process, this paper introduces an array architecture for the implementation of fuzzy vector quantization (FVQ). The arrayarchitecture, which consists of 4,096 processing elements (PEs), provides a computationally efficient solution by employing an effective vector assignment strategy during the clustering process. Experimental results indicatethat the proposed parallel implementation providessignificantly greater performance and efficiency than appropriately scaled alternative array systems. In addition, the proposed parallel implementation provides 1000x greater performance and 100x higher energy efficiency than other implementations using today's ARMand TI DSP processors in the same 130nm technology. These results demonstrate that the proposed parallel implementation shows the potential for improved performance and energy efficiency.

• Bulletin of the Korean Mathematical Society
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• v.34 no.2
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• pp.155-171
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• 1997

Let $M^n$ be a connected subminifold of a Euclidean space $E^m$, equipped with the induced metric. Denoty by $\Delta$ the Laplacian operator of $M^n$ and by x the position vector. A well-known T. Takahashi's theorem [13] says that $\delta x = \lambda x$ for some constant $\lambda$ if and only if $M^n$ is either minimal subminifold of $E^m$ or minimal submanifold in a hypersphere of $E^m$. In [9], O. Garay studied the hypersurfaces $M^n$ in $E^{n+1}$ satisfying $\delta x = Dx$, where D is a diagonal matrix, and he classified such hypersurfaces. Garay's condition can be seen as a generalization of T.

• Journal of the Korean Mathematical Society
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• v.36 no.4
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• pp.649-662
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• 1999

A random vector =(X1,…, Xn) is conditionally weakly associated if and only if for every pair of partitions 1=(X$\pi$(k+1),…,X$\pi$(k)), 2=(X$\pi$(k+1),…,X$\pi$(n)) of P(1$\in$A│2$\in$B, $\theta$$\in$I) $\geq$P$\in$A│$\theta$$\in$I whenever A and B are open upper sets and $\pi$ is any permutation of {1,…,n}. In this note we develop some concepts of conditional positive dependence, which are weaker than conditional weak association but stronger than conditional positive orthant dependence, by requiring the above inequality to hold only for some upper sets and applying the arguments in Shaked (1982).

• Journal of the Korean Statistical Society
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• v.25 no.2
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• pp.243-253
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• 1996

Let X and Y be random vectors in R$^{n}$ . A random vector X is 'more associated' than Y if and only if P(X $\in$ A ∩ B) - P(X $\in$ A)P(X $\in$ B) $\geq$ P(Y $\in$ A ∩ B)-P(Y $\in$ A)P(Y $\in$ B) for all open upper sets A and B. By requiring the above inequality to hold for some open upper sets A and B various notions of positive dependence orderings which are weaker than 'more associated' ordering are obtained. First a general theory is given and then the results are specialized to some concepts of a particular interest. Various properties and interrelationships are derived.

• Journal of the Chungcheong Mathematical Society
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• v.7 no.1
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• pp.59-67
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• 1994

Let (${\Omega},{\Sigma},{\mu}$) be a measure space. A function $f:{\Omega}{\rightarrow}X$ is said to be equioscillated if for each set $A{\in}{\Sigma}$ of positive measure and for each ${\epsilon}$ > 0, there is a measurable subset B of A of positive measure such that the inequality s$sup_{{\omega}{\in}B}x^*f({\omega})-inf_{{\omega}{\in}B}x^*f({\omega})$ < ${\epsilon}$ holds for every $x^*$ with $||x^*||{\leq}1$. Strong measurability of a vector valued function is characterized using equioscillation.

• Bulletin of the Korean Chemical Society
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• v.15 no.1
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• pp.64-74
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• 1994

The nonstoichiometric chalcogenides with NaCl-type structure were prepared and the physical and structural properties were studied. The homogeneous range and the structural change were studied based on X-ray powder diffractions using Rietveld-type full-profile fitting technique. Wide homogeneous ranges were observed in Y-S and Y-Se systems, and relatively narrow homogeneous ranges were observed in Yb-S and Yb-Se systems. Both in $Yb_{1-x}S\;and\;Yb_{1-x}Se$, a vacancy ordering transition occurred in (111) plane direction. The ordered superstructure had cubic symmetry(Fm$\bar{3}m) with doubled unit cell "a" parameter compared to the original NaCl-type. The superlattice developed in a continuous second-order transitiion was characterized by the reduced waved vector k= $(a^*+b^*+c^*)/2$. Y-S system had metallic, and YSe, YbSe system had semiconducting properties in their homogeneous ranges. It was observed that the change of electronic transport properties in extended homogeneous range did not depend on the relativeratio of metal to nonmetal, but on the quantities of vacancies.