• 생약학회지
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• 제48권4호
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• pp.298-303
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• 2017

To know the anti-oxidative effect of Epimedii Herba (Berberidaceae), the methanol extract of this plant was investigated by using a Caenorhabditis elegans model system. The methanol extract of this plant showed relatively significant DPPH radical scavenging and superoxide quenching activities. The ethyl acetate soluble fraction of Epimedii Herba (EHE), which showed the most potent DPPH radical scavenging and superoxide quenching activities, was tested on its effects on superoxide dismutase (SOD), catalase, intracellular ROS, and oxidative stress tolerance in Caenorhabditis elegans. Furthermore, in order to verify that regulation of stress-response genes is responsible for the increased stress tolerance of the EHE treated C. elegans, we checked SOD-3 expression using a transgenic strain. As a result, the EHE increased SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Besides, EHE-treated CF1553 worms showed higher SOD-3::GFP intensity than that of non-treated controls.

• 한국잠사곤충학회지
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• 제8권
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• pp.51-62
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• 1968

1. 수원지방에서 다화성잠저승의 세대회수는 5~6회이다. 2. 다화성잠저승의 란, 유충, 용, 기간은 대체적으로 2일, 8일, 18일 간이다. 3. 기상조건 특히 온도에 대하여 본충의 세대기간은 큰 영향을 받는다. 각세대중에서 가장 짧은 세대는 제3세대로서 21일 14시간이고 가장 긴 세대는 제5세대로서 약40일 간이다. 4. 가잠에 기생한 본충의 유충은 탈출후 3.5cm-10.5cm 토중으로 들어가 화용한다. 5. 다화성잠저승은 용태월동을 하지 않고 유충태월동을 한다고 생각된다. 6. 실외사육에 있어서 본충에 대한 피해는 27.9% 였다. 7. 견잠에 있어서 다화성잠저승의 피해를 받을지라도 영견가능하고 견질에 대하여 큰 영향이 없다. 8. 수견후 본충의 전국 피해조사에 있어서 춘잠기가 6.36%, 추잠기가 4.09%, 년간 5.25%였다.

• Parasites, Hosts and Diseases
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• 제40권2호
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• pp.83-88
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• 2002

The 8 kDa antigenic protein of Clonorchis sinensis was partially purified by ammonium sulfate precipitation and subsequently by a column chromatographic steps. The purified protein was separated into 7 and 8 kDa protein bands through SDS-tricine gel electrophoresis, while the protein was fecund to migrate to a 8 kDa band in 7.5-15% SDS-PAGE. The molecular weight of the antigen was estimated to be 110 kDa by Superose 6 HR 10/30 gel filtration. The purified antigen strongly reacted with the human sera of clonorchiasis. The hyperimmune sera of BALB/c mice immunized against the 8 kDa protein were reacted with both the crude extract and the excretory-secretory product of adult worms, but not with the metacercarial extract. Immunohistochemical staining demonstrated that the protein was distributed to the tegument and subtegumental cells and also to the seminal receptacle. The present findings suggest that the 8 kDa protein is a partition of the multicomplek protein originating from various organs of adult C. sinenis, and that it is composed of several 7 and 8 kDa proteins.

• Parasites, Hosts and Diseases
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• 제35권3호
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• pp.211-214
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• 1997

중국산 수입 미꾸리에서 검출한 돼지악구충(Gnothostoma hispidum)의 유충(early third-stage larvae: EL3)을 10∼20개씩 비단잉어, 가물치 황소개구리. 마우스 및 흰쥐 등에 각각 경구 감염시킨 후 감수성을 관찰하였다. 총 40마리 및 45마리의 유충을 감염시킨 비단잉어 4마리와 가물치 3마리에서는 충체가 한 마리도 검출되지 않았다 유충을 총 30마리 감염시킨 황소개구리 3마리에서는 감염 후 2주에 위 및 장(8마리)과 간(1마리)에서 각각 검출되었는데 미꾸리에서 검출된 것과 같은 EL3이었다. 마우스 5마리에 유충을 각각 10마리씩 감염시키고 4주 후에 감염여부를 검사하였던 바. 총 37마리(74%)가 근육(31마리), 간(5마리). 신장(1마리) 등에서 검출되었다 유충 115마리를 흰쥐 9마리에 감염시키고 4주 및 8주 후에 충체를 회수하였던 바 총 40마리(34.8%) 가 모두 근육에서 검출되었다 이상의 결과로 중국산 수입 미꾸리에서 검출한 돼지악구충의 제3기 유충이 어류나 개구리에서 보다 마우스와 흰쥐에 더 잘 감염됨을 알 수 있었다.

• Parasites, Hosts and Diseases
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• 제47권2호
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• pp.189-191
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• 2009

Four feral cats and a raccoon dog purchased from a local collector on Aphaedo Island, Shinan-gun, where human Gymnophalloides seoi infections are known to be prevalent, were examined for their intestinal helminth parasites. From 2 of 4 cats, a total of 310 adult G. seoi specimens were recovered, Other helminths detected in cats included Heterophyes nocens (1,527 specimens), Pygidiopsis summa (131), Stictodora fuscata (4), Acanthotrema felis (2), Spirometra erinacei (15), toxocarids (4), and a hookworm (1). A raccoon dog was found to be infected with a species of echinostome (55), hook-worms (7), toxocarids (3), P. summa (3), and S. erinacei (1). No G. seoi was found in the raccoon dog. The results indicate that feral cats and raccoon dogs on Aphaedo are natural definitive hosts for intestinal trematodes and cestodes, including G. seoi, H. nocens, and S. erinacei, It has been first confirmed that cats, a mammalian species other than humans, play the role of a natural definitive host for G. seoi on Aphaedo Island.

• Parasites, Hosts and Diseases
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• 제52권3호
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• pp.273-280
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• 2014

The changing patterns of goblet cell hyperplasia, intestinal epithelial cell turnover, and intestinal motility were studied in ICR and C57BL/6 mice infected with Gymnophalloides seoi (Digenea: Gymnophallidae). Whereas ICR mice retained G. seoi worms until day 7 post-infection (PI), C57BL/6 mice showed a rapid worm expulsion within day 3 PI. Immunosuppression with Depo-Medrol significantly delayed the worm expulsion in C57BL/6 mice. Goblet cell counts were increased in both strains of mice, peaking at day 1 PI in C57BL/6 mice and slowly increasing until day 7 PI in ICR mice. In C57BL/6 mice infected with G. seoi, newly proliferating intestinal epithelial cells were remarkably increased in the crypt, and the increase was the highest at day 1 PI. However, in ICR mice, newly proliferating intestinal epithelial cells increased slowly from day 1 to day 7 PI. Intestinal motility was increased in G. seoi-infected mice, and its chronological pattern was highly correlated with the worm load in both strains of mice. Meanwhile, immunosuppression of C57BL/6 mice abrogated the goblet cell proliferation, reduced the epithelial cell proliferation, and suppressed the intestinal motility. Goblet cell hyperplasia, increased intestinal epithelial cell turnover, and increased intestinal motility should be important mucosal defense mechanisms in G. seoi-infected C57BL/6 mice.

• 한국동물위생학회지
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• 제40권3호
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• pp.169-175
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• 2017

Acanthocephalan worms were harvested from the posterior intestines of the cultured marine fishes from January to July 2016 in a fish market located in Daejeon metropolitan city, Republic of Korea. Totally 450 cultured fishes (rock fish 100, olive flounder 250, red sea bream 100) were surveyed. Of the 100 red sea bream fish, 37 fishes (37%) were diagnosed as positive for Longicollum pagrosomi by light, electron microscopic and molecular examination. The number of worm was 25~78 (mean $51{\pm}13$). However, we can't found any worm from the cultured rock fish and olive flounder. After sequencing, none of Pomphorhynchidae family are not identical based on 18S rRNA gene, and this data were identified with the first report for 18S rRNA gene sequence of L. pagrosomi. Furthermore, we confirmed that L. pagrosomi of the cultured red sea bream in Republic of Korea is very common parasite.

• 대한수의학회지
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• 제34권1호
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• pp.127-134
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• 1994

As a series of studies to investigate the effect of immunosuppression on Ascaris suum infection in undefinitive hosts, a delicate relationship between host and parasite, in the present studies golden hamsters were alloted to experiment 1(normal undefinitive host group) and experiment 2(immunosuppressive group treated with prednisolone acetate) and inoculated with a single dose of 1,500 embryonated Ascaris suum eggs. The recovery rates, sizes and features of the larvae and immunological responses in the hamsters were chronologically monitored according to somatic migration. In both experiments, the larvae failed to develop into the adults, but the more and larger larvae were observed for a longer period from experiment 2 in comparison with experiment 1. The numbers of the mast cells in the small intestinal mucosa and mesenteric lymph nodes, of the goblet cells in the small intestinal mucosa and of T-cells in the mesenteric lymph nodes, spleens and cardiac blood from experiment 2 were fewer than those from the experiment 1. In general, increasing of these cells followed by expulsion of the worms in the both groups. Profound leukopenia due to lymphopenia was found through trial period in experiment 2. Considering the experimental results, development or expulsion mechanism of somatic migrant larvae may be related to lymphopenia and temporary increasing tendency of the mast cells, the goblet cells and T-cells. In addition, patent infection of A suum in the hamsters was not obviously observed in spite of immunosuppression by prednisolone acetate.

• 대한수의학회지
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• 제33권3호
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• pp.465-469
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• 1993

낭미충(Cysticercus cellutosae)에 자연감염된 돼지의 각 장기를 조직학적으로 검사하였던 바 다음과 같은 결과를 얻었다. 피막을 형성하는 낭미충은 골격근, 파하직, 심장 및 뇌조직에서 관찰되었다. 조직학적 소견으로는 골격근의 근막내와 심장 심외막하에서 낭충주위는 피막의 형성과 함께 급성 염증반응이 인정되었고 부위에 따라서는 교원섬유 및 선유아세포의 증식에 의한 두터운 피막이 관찰되었고 인접한 골격근 또는 심근과 견고하게 부착된 예도 있었다. 피막주위에서는 호산구, 임파구, 대식세포의 침윤이 부위에 따라 경미하거나 또는 심한 형태로 다양하였다. 대뇌의 연막하에 형성된 피막주위에는 혈관과 결합조직의 증식이 현저하였고, 혈관주위 원형세포의 침윤과 임파결절 모양의 구조가 인정되었다. GFAP 면역반응은 혈관주위를 따라 GFAP 양성의 섬유가 잘 발달되었고 피막낭 외측을 따라 전체를 둘러싸는 경항이었다. 결론적으로 유구낭미충 감염돼지의 조직소견은 감염 장기에 따라 염증반응이 다양하고 낭충의 피막은 감염 초기에 형성된 것으로 추정되었다.

• Applied Microscopy
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• 제15권1호
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• pp.101-111
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• 1985

A study was undertaken to observe the distribution and ultrastructure of the sensory papillae of the liver and lung flukes which was obtained from experimentally infected rats and dogs. For this study, the rats were artificially infected with metacercariae isolated from Pseudorasbora parve, and the dogs were artificially infected with metacercariae isolated from Cambaroides similis. The liver flukes (Clonorchis sinensis) were collected from the bile ducts of the rats which were autopsied 5 weeks later since infection. The lung flukes (Paragonimus westermani) were collected from the lung of the dogs which were autopsied 3 months later since infection. With the collected worms, ultrastructure of sensory papillae was studied by means of a scanning and transmission electron microscope. The liver flukes were compared with the lung flukes. The results of the observation are as follows. 1. The sensory papillae of the liver flukes was classified into 3 types and most of sensory papillae were distributed on the oral sucker. 2. The sensory papillae of the lung flukes were distinguished 8 types. The sensory papilla type VIII was specifically distributed on the oral sucker and type I, II were distributed on the tegumental surface of the worm. The sensory papillae of the lung flukes were mostly observed between oral sucker and ventral sucker of the worm excusively 3. The sensory papillae of the liver flukes were distributed around the sucker while those of the lung flukes were developed between spine. 4. The sensory papillae were formed by many tegumental ridge in the liver flukes while was made of many small sensory papillae in the lung flukes. 5. The sensory cilia were observed between tegumental ridges in the sensory papilla of the liver flukes and also in lung flukes. And they are alike in that respect. 6. The sensory papillae were not developed in the posterior part of the liver and lung flukes but mostly nerve endings were formed in the tegumental syncytium, and it was connected with neuropile.