Three lepidopteran insect pests of the legume pod borer, Maruca vitrata (Lepidoptera: Pyralidae), the soybean pod worm, Matsumuraeses phaseoli (Lepidoptera: Tortricidae) and an Ostrinia spp. (Lepidoptera: Pyralidae) attacking adzuki bean, Vigna angularis, were confirmed as the major insect pests during the reproductive developmental stage of adzuki bean, and M. vitrata existed dominantly. Almost all plants in the adzuki bean field were injured by at least one of the three species, and $15{\sim}60%$ of reproductive organs were injured. Unbloomed inflorescence occurred continuously through the reproductive stage of adzuki bean. While the flower was many at the early stage and its number soon decreased, the pod was few at the early stage, but its number increased soon and maintained at a constant level. The results suggested that the adzuki bean injured by insect pests compensate the flower loss by developing new inflorescence, but the compensation do not cause the development of new pods. While M. vitrata and M. phaseoli were observed in flowers, pods and stem mainly during the first half of reproductive stage of adzuki bean, Ostrinia spp. was observed only in pods and stem during the second half. In addition, while all instars of larvae of M. vitrata were observed, larvae between the third and fifth instars for M. pahseoli and Ostrinia spp. were observed.
Kim, Seon-Gon;Kim, Do-Ik;Ko, Suk-Ju;Kang, Beom-Ryong;Kim, Hong-Jae;Choi, Kyeong-Ju
Korean journal of applied entomology
/
v.48
no.1
/
pp.81-86
/
2009
Economic injury level and control thresholds for the management of beet army worm, Spodoptera exigua (Lepidoptera: Noctuidae) were evaluated on chinese cabbage of two different planting time. Two inoculation times were tested for each planting and the number of inoculated larva was 10, 20, 40, 80, respectively. Damages of leaves by first inoculation were 63.2% after eight days planting on 80 larva inoculation plot. By the second inoculation, those were below 50% after 20 days planting on the end of September. The linear relationships between population density and yield reduction were as following; Y = -10.62x + 867.9 ($R^2\;=\;0.643$) for 5 days and Y=-6.432x + 1074 ($R^2\;=\;0.720$) for 20 days. Based on these results the economic injury level was 5.4 larva for five days and 9.0 larva for 20 days per 20 chinese cabbage. The control thresholds calculated by 80% level of economic injury level were 4.3 and 7.2 larva, respectively.
This study was aimed to investigate the enzyme-histochemical localization and characteristics of alkaline and acid phosphatase extracted from adult of Echinostoma hortense. Using the Gomori calcium stain and the Gomori lead nitrate satin method, we found that the alkaline and acid phosphatases were localized mostly in the intestine, vitellaria and pharynx of Echinostoma hortense. The three isozymes of alkaline phosphatase and two isozymes of acid phosphatase were separated from Echinostoma hortense by electrophoresis. The isozymes of alkaline phosphatase were 145.9, 207.5, 220.8 kDa and the isozymes of acid phosphatase were 179.5 and 209.4 kDa. The activity of alkaline phosphatase was denatured completely after heating at 9$0^{\circ}C$ for 12 seconds. The optimum pH and temperature for activity of alkaline phosphatase were about pH 9 and 4$0^{\circ}C$, while the optimum pH for activity of acid phosphatase was about pH 5. The maximum activity of alkaline phosphatase was at 189 unit, but maximum activity of acid phosphatase was at 71 unit As the result from above, we observed that alkaline and acid phosphatases funtion mainly in the alimentary tract and vitellaria. Echinostoma hortense performs the parasitism in the intestine of host by using proper isozyme of phosphatase.
The present study was performed with morphological and molecular analysis (cox1 and nad1 mitochondrial genes) to identify the proglottids of spirometrid tapeworm found in the stool of an African lion, Panthera leo, in the Serengeti plain of Tanzania. A strand of tapeworm strobila, about 75 cm in length, was obtained in the stool of a male African lion in the Serengeti National Park ($34^{\circ}$ 50' E, $02^{\circ}$ 30' S), Tanzania, in February 2012. The morphological features of the adult worm examined exhibited 3 uterine coils with a bow tie appearance and adopted a diagonal direction in the second turn. The posterior uterine coils are larger than terminal uterine ball and the feature of uteri are swirling rather than spirally coiling. The sequence difference between the Spirometra species (Tanzania origin) and S. erinaceieuropaei (GenBank no. KJ599680) was 9.4% while those of S. decipiens (GenBank no. KJ599679) differed by 2.1% in the cox1 and nad1 genes. Phylogenetic tree topologies generated using the 2 analytic methods were identical and presented high level of confidence values for the 3 major branches of the 3 Spirometra species in the cox1 gene. The morphological and molecular findings obtained in this study were nearly coincided with those of S. ranarum. Therefore, we can know for the first time that the African lion, Panthera leo, is to the definitive host of this tapeworm.
Journal of agricultural medicine and community health
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v.10
no.1
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pp.26-35
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1985
The present study was undertaken to evaluate the prevalence rate of Taenia species infection and distribution of taeniasis caused by Taenia solium among Koreans in Seoul and Cheju Do In Korea during the period from August to December 1984. A total of 4,256 stool specimens from the students of Mapo Ku and 360 stool specimens from the inhabitants of Guro-Ku in Seoul was collected and examined by cellophane thick smear technique. On the other hand 1,015 stool specimens from the students of Jocheon Myun and 265 specimens from the students of Jocheon Myun and 265 specimens from the inhabitants of Aeweol Myun and Gujwa Myun in Cheju Do were also examined. The results were summarized as follows ; Four (0.1%) out of 4.256 students were positive and 2 (0.6%) out of 360 inhabitants in Seoul were positive (Table 1, 2). Positive rates of taeniasis in Cheju Do were 1.7% (17) out of 1,015 students and 12.5% (33) out of 5 villagers (Table 3, 4). In order to observe the distribution of Taenia solium infection, the scolex or a part of Taenia spp. were collected from the stool of positive cases by anthelmintic treatment. For the species identification, expelled proglottides were examined microscopically by the number of branches of the uterus, presence of vaginal sphincter or the accessory ovarian lobe etc. Three cases were infected with Taenia solium among 6 egg positive cases in Seoul. But only 1 case was infected with Taenia solium out of 7 students taking anthelmintics voluntarily by recommendation of Korean Association for Parasite Eradication (KAFPE). On the other hand, among 32 cases of egg positive cases of this study and 26 cases of KAFPE in Cheju Do, 13 cases (22.4%) were infected with Taenia solium. But 13 cases who were not examined and complained expulsion of proglottides in their stool were infected with Taenia saginata (Table 5). Among 62 persons infected with Taenia saginata, only 4 cases did not know their infection of this worm. Rut in 17 cases with Taenia solium, 7 persons did not know their infection until stool examinations were performed (Table 6).
While various examples of rationale or benefits of ascariasis control could justify a necessity of control activity in a community, few practical index other than the e99 Prevalence or intensity of infection has been used to evaluate the efficacy obtained by the control activity. A literature-based retrospective study was undertaken to provide some numerical data on the decreasing morbidity of surgical ascariasis as a consequence of ascariasis control in Korea. A thorough review of literatures (1959∼1990) on biliary and other surgical complications of ascariasis reported from general hospitals revealed that their incidence has been decreasing very proportionally to the national figures of its e99 Prevalence and worm burden. For example, according to the remarkable decreases of the prevalence of ascariasis, there is an evident decreasing tendency of the proportion(%) of ciliary ascariasis cases among all biliary surgical patients and the proportion of biliary ascariasis cases among all biliary stone patients in general hospitals. It is highly suggested that the Korean people have been much benefited by the control activity of ascariasis executed at a national scale since 1960s, especially in terms of incidence of biliary and other surgical complications of ascariasis.
The complete life cycle of Echinostoma hertense has been maintained in the laboratory, using Lymnaea persia snails and Rana nigromaculata tadpoles as the first and second intermediate hosts. ICR mice was used as the definitive host. Within the egg of 5. hotense, the miracidium was fully matured in 13 days of incubation at $29~30^{\circ}C$. The miracidium was $93.8{\times}53.6{\;}{\mu\textrm{m}}$ in average size, covered with numerous cilia of $7~11{\;}{\mu\textrm{m}}$ length. The epidermal plates were arranged in 6-8-4-2 formula. The first generation rediae ($1.19{\times}0.27{\;}mm$ in average size) were observed in 14 days after miracidial challenge to the snails, and the second generation rediae ($1.40{\times}0.26{\;}mm$ in average size) in 30 days. The average sixte of the cercaria was $295.5{\times}145.0{\;}{\mu\textrm{m}}$. Their head crown was poorly developed, and collar spines were not yet observed. After a cercarial challenge to the tadpoles, all of the tadpoles became infected and the average worm recovery rate was 88.5%. The majority of the metacercariae (75.5%) were recovered from the muscle of the tadpole's posterior body and the rest (24.3%) from their gills. The metacercariae from the tadpoles were elliptical, and $167.7{\times}129.9{\;}{\mu\textrm{m}}$ in average size. The recovery rate of adults from the mice was difFerent by the age of the metacercariae grown in the tadpoles. The metacercariae younger than 5 hrs could not infect mice whereas those older than 6 hrs could infect mice. The recovery rate became higher as the metacercaria matured, with the peak recovery rate of 90.0 % at the metacercarial age of 9 days. Thereafter the recovery rate decreased to 55.0% at the age of 50 days. As shown by the above results, the whole life cycle of E. hcrtense has been completed in the laboratory. At least 55~58 days were required to maintain one egg-to-egg cycle of E. hortense.
Journal of agricultural medicine and community health
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v.16
no.2
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pp.154-164
/
1991
Albendazole is a broad spectrum anthelmintics with high activity against intestinal nematodes as well as tremtodes and cestodes infections. But so far wormicidal effect of albendazole against Paragonimus westermani is uncertain. The present study undertaken to observe the morphological changes of Paragonimus westermani which was obtained from experimentally infected with metacercariae isolated from Cambaroides similis and 9 of them were given albendazole 2 doses of 25mg/kg daily for the 2 days and 4 days from the day of 20 weeks after infection. The Paragonimus werms were collected from the lung of the cats which were autopsied at 24 hours after treatment. The fine structures of the collected worms were studied by means of scanning and transmission electron microscopy. The findings of the observations were compared with those of untreated worms. The results are as follows : 1) In the scanning electron microscopic(SEM) observations, the worms obtained from 2 days treated group showed many blebs which were formed on the surface of damaged tegument in between two suckers. Protrusion of oral sucker typically observed and surrounding tegumental ridges were damaged. 2) The worms obtained from 4 days treated group showed marked contraction revealing half size of normal worms. 3) The transmission electronmicroscopic(TEM) observations indicated that large blebs of irregular shapes were formed by the destruction of tegumental ridge of tegumental syncytium and also showed degeneration of mitochondriae. 4) Destruction of tegumental syncytium formation of blebs were usually observed in the ventral side of the worms. 5) Differentiation of tegumental layer and basement layer muscular layer and parenchymal layer around two suckers of worms obtained at 4 days treated group was difficult. 6) Many host cells invaded and destroyed the degenerated tegument by the occurrence of blebs or the exposed muscular layer of the worm after exfoliation of the tegument.
The aim of the present study is to investigate the interannual variabilities of the East Asia monsoon rainfall associated with the global sea surface temperature anomaly(SSTA). For this study, the summer rainfall(from June to August) over the twenty-eight period of 1961-1988 were analyzed with being divided by nine-subregions over East Asia including Korea, China and Japan. From the analysis of the principal modes explaining the interannual variation, the interannual variabilities of summer rainfalls in South Japan and Korea are larger than those of the other subregions of the East Asia. There is a strong negative correlation between the summer rainfalls of south China and Korea. In this study, the relationship between the summer monsoon of each subregion and SSTs of the tropical NINO regions, of western Pacific warm pool, and of the subtropical ocean were investigated. The longitudinal sections of the lagged cross correlations of the summer rainfal1 anomaly in (a) Korea and (b) south China, and the monthly SSTA in the equatorial(averaged from 65 to 6N) Pacific were analyzed. The negative maximum correlation pattems of Korea''s stammer rainfal1 and SSTs over the eastern Pacific is transfered to positive maximum conrlation over central Pacific region with a biennial periodicity. In South China, the significant positive correlations are found at -12 month lag over the eastern Pacific and maximum negative correlation at 16 month lag over the central Pacific with the quasi-biennial oscillation. But the correlation coefficient reverses completely to that in Korea. In order to investigate the most prevailing interannual variability of rainfall related to the favored SSTA region, the lagged cross correlations between East Asia rainfall and SSTs over the moO regions(NINO 1+2(0-105, 90W-80W), NINO 3(5N-5S, 150W-90W), NINO 4(5N-5S, 160E-l50W) and the western Pacific worm pool (5N-5S, 120E-l60E) were analyzed. Among the lagged cross-correlation cycles in NINO regions, the maximum correlations for the negative lagged months prevail in NINO 1+2 and NINO 3, and the cross correlations for the positive lagged months NINO 4. It is noteworthy that correlation between the western Pacific warm pool SSTA and the monsoon rainfall in Korea and South China have the maximum value at negative 4 month lag. The evolution of the correlation between the East Asia monsoon rainfall and SSTA is linked to the equatorial convective cluster and related to northward propagating situation, and raising the possibility that the East Asia monsoon precipitation may be more fundamentally related to the interaction of intraseasonal oscillations and the sub-regional characteristics including the surface boundary conditions and the behavior of climatological air mass.
The aim of the present study is to investigate the interannual variabilities of the East Asia monsoon rainfall associated with the global sea surface temperature anomaly(SSTA). For this study, the summer rainfall(from June to August) over the twenty-eight period of 1961-1988 were analyzed with being divided by nine-subregions over East Asia including Korea, China and Japan. From the analysis of the principal modes explaining the interannual variation, the interannual variabilities of summer rainfalls in South Japan and Korea are larger than those of the other subregions of the East Asia. There is a strong negative correlation between the summer rainfalls of south China and Korea. In this study, the relationship between the summer monsoon of each subregion and SSTs of the tropical NINO regions, of western Pacific warm pool, and of the subtropical ocean were investigated. The longitudinal sections of the lagged cross correlations of the summer rainfal1 anomaly in (a) Korea and (b) south China, and the monthly SSTA in the equatorial(averaged from 65 to 6N) Pacific were analyzed. The negative maximum correlation pattems of Korea's stammer rainfal1 and SSTs over the eastern Pacific is transfered to positive maximum conrlation over central Pacific region with a biennial periodicity. In South China, the significant positive correlations are found at -12 month lag over the eastern Pacific and maximum negative correlation at 16 month lag over the central Pacific with the quasi-biennial oscillation. But the correlation coefficient reverses completely to that in Korea. In order to investigate the most prevailing interannual variability of rainfall related to the favored SSTA region, the lagged cross correlations between East Asia rainfall and SSTs over the moO regions(NINO 1+2(0-105, 90W-80W), NINO 3(5N-5S, 150W-90W), NINO 4(5N-5S, 160E-l50W) and the western Pacific worm pool (5N-5S, 120E-l60E) were analyzed. Among the lagged cross-correlation cycles in NINO regions, the maximum correlations for the negative lagged months prevail in NINO 1+2 and NINO 3, and the cross correlations for the positive lagged months NINO 4. It is noteworthy that correlation between the western Pacific warm pool SSTA and the monsoon rainfall in Korea and South China have the maximum value at negative 4 month lag. The evolution of the correlation between the East Asia monsoon rainfall and SSTA is linked to the equatorial convective cluster and related to northward propagating situation, and raising the possibility that the East Asia monsoon precipitation may be more fundamentally related to the interaction of intraseasonal oscillations and the sub-regional characteristics including the surface boundary conditions and the behavior of climatological air mass.
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