• Title/Summary/Keyword: Wome

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Intravenous and Intra-arterial Delivery of Plasmid DNA/Cationic Lipiodol Emulsion Complexes

  • Chae, Min-Jung;Chung , Hes-Son;Kwon, Ick-Chan;Chung, Jin-Wook;Park, Jae-Hyung;Sohn, Young-Taek;Jeong, Seo-Young
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.226.3-227
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    • 2003
  • A cationic lipid emulsion (o/w) containing lipiodol and 1, 2-dioleoyl-sn- glycero-3-trimethylammonium-propane (DOTAP) has been prepared as a gene delivery system. In order to increase the transfection efficiency of the lipiodol emulsion, 1 2-dioleoyl-sn-glycero-3-phospho-ethanolamine (DOPE) and polyoxyethylene sorbitan monooleate (Tween 80) were incorporated as additional lipids. By including DOPE and Tween 80, the cationic emulsion became a more potent gene carrier under in vitro condition in the presence of serum, and under in vivo condition. (omitted)

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Efficacy of Duplex-nested PCR and Fluorescent PCR in the Preimplantation Genetic Diagnosis for Duchenne Muscular Dystrophy (근이영양증에 대한 착상전 유전진단에서 Duplex-nested PCR과 Fluorescent PCR 방법의 효용성)

  • Lee, Hyoung-Song;Choi, Hye Won;Lim, Chun Kyu;Park, So Yeon;Kim, Jin Young;Koong, Mi Kyoung;Jun, Jin Hyun;Kang, Inn Soo
    • Clinical and Experimental Reproductive Medicine
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    • v.32 no.1
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    • pp.17-26
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    • 2005
  • Objective: Preimplantation genetic diagnosis (PGD) is reserved for couples with a risk of transmitting a serious and incurable disease, and hence avoids the undesirable therapeutic abortion. In this study, we evaluated the efficacy of PGD for Duchenne muscular dystrophy (DMD) cases by the fluorescent PCR with polymorphic linked markers and the conventional duplex-nested PCR methods. Methods: Biopsy of one or two blastomeres was done from the embryos fertilized by ICSI on the third day after fertilization. We performed two cases of PGD-DMD by the duplex-nested PCR for the causative mutation loci and the SRY gene on Y chromosome. The triplex fluorescent PCR for the mutation loci, the SRY gene and the polymorphic microsatellite marker on X chromosome was applied for two cases of PGD-DMD. Results: By the duplex-nested PCR, successful diagnosis rate was 95.5% (21/22), but we could not discriminate the female embryos whether normal or carrier in this X-linked recessive disease. However, the triplex fluorescent PCR method showed 100% (27/27) of successful diagnosis rate, and all female embryos (n=17) were distinguished normal (n=10) from carrier (n=7) embryos. Unaffected and normal embryos were transferred into mother's uterus after diagnosis. A healthy normal male was achieved after PGD with the duplex-nested PCR method and a twin, a male and a female, were delivered with triplex fluorescent PCR method. The normality of dystrophin gene was confirmed by amniocentesis and postnatal genetic analysis in all offsprings. Conclusion: The fluorescent PCR with polymorphic marker might be useful in improving the specificity and reliability of PGD for single gene disorders.

A Human Metabolic Study for Determination of Daily Requirement of Riboflavin (한국인의 리보플라빈 일일필요량 측정을 위한 인체대사연구)

  • 윤진숙
    • Journal of Nutrition and Health
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    • v.22 no.6
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    • pp.507-515
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    • 1989
  • Ten healthy wome 2-~25 years participated in a 5-week metabolic study to investigate the daily riboflavin requirement of Korean women. Three daily menu and meal patterns were used. Low riboflavin(LR) diet provided riboflavin at a level of 0.71mg / 1000Kcal based on food composition table. High riboflavin(HR) diet provided 1.14mg / 1000Kcal. The riboflavin status was assessed by erythrocyte glutathione reductase activity coefficient(EGRAC) and urinary exretion of riboflavin. Mean EGRAC of LR period and HR period were 1.17+0.08 and 1.01+0.03, repectively. There were discrepanices of riboflavin content of experimental diet between chemical analysis and calculation by Korean food composition table. Urinary riboflavin excretion of subjects was acceptable over the whole study period. It appeared that 0.71mg / 1000Kcal is requirement of riboflavin to maintain EGRAC withing normal range. Thus, it is suggested that current recommendation of 0.6mg / 1000Kcal of riboflavin intake may not be adequate as RDA level in Korea.

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A Study on 3D Modeling Technique using Parametric Representation (계수 표현 모델을 이용한 3차원 모델링 기법에 관한 연구)

  • Kim Tae-Eun
    • Journal of Digital Contents Society
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    • v.5 no.3
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    • pp.176-179
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    • 2004
  • Superquadrics can represent various and complex 3D object with only wome parameters(size, position, deformation etc). So if we use both superquadrics and deformed superquadrics, we can reoresent more realistic 3D object which are existed in real world. In this paper we use the Z-buffer algorithm and stencil buffer together because this is very useful when the wuperquadric primitives are combined. The fundamental ideas are illustrated with a number of tables and figures.

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Development of the Basic brassiere pattern - focusing on 75A - (브래지어 원형 설계 - 75A를 중심으로-)

  • Park, You-Shin;Jang, Eun-Young
    • Journal of Fashion Business
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    • v.12 no.1
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    • pp.109-119
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    • 2008
  • The purpose of this study is to dvelop the basic pattern of wome which are fitting for 75A. The collected data were statistcally processed usig the SPSS 10.0 for Mans Standard Deiation, Cronbach's $\alpha$, etc. This study suggested a size chart for body measurement for constructing basic brssiere patterns with size of 75A. The construction of this experimental brassiere were lower cup, upper cup, front panel and wing and characterized by a round cup wired as well as a full cup embrcing the entire breasts. The drafting method for constructing basic brassiere patterns for 75A was completed by selecting a total of 11subjects, and two wearing tests were carried out with the subjects. the dimensions and shapes of the basic brassiere pattern suggested in this research proved to be fitting and to have a wearing effect that brings together and supports the breasts.

The Effect of Public Health Center Program participation on Metabolic Syndrome and Risk of Disease in Middle- aged and Elderly Women (보건소 건강교실 참여가 중,고령 여성의 대사증후군과 질병 발병 위험에 미치는 효과)

  • Lee, Yong-Soo
    • Journal of the Korea Convergence Society
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    • v.7 no.6
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    • pp.317-325
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    • 2016
  • The purpose of this study was to examine the effect of the education intervention program as a nutrition program and exercise for the improvement of metabolic syndrome in women who voluntarily participated in health program in public health centers over 30 years of age. The subjects of this study were 120 women who completed the 12 - week obesity clinic program among the 400 obesity clinic participants in the northeastern of Seoul. Exercise and nutrition programs were administered on a daily basis for 1 hour every week for 12 hours with exercise and nutrition for 1 week to assess the effect of the participants on the risk factors of metabolic syndrome in health program. The metabolic syndrome and the risk of the disease were examined by analyzing the metabolic syndrome risk factors, change magnitude and change rates before and after the intervention. Waist circumference(p <.001), systolic blood pressure (p <.001), diastolic blood pressure (p <.001) and body mass index (p <.001) decreased during the 12 - week health program. Body weight (p <.01), waist circumference (p <.05), BMI (p <.01) and fat mass (p <.05) decreased more in the age group below 50 years.

The Effect of Changing the Order of Exercise Types on Body Composition and Blood Lipid in Obese Women (복합운동의 운동유형 순서변화가 비만여성에게 미치는 영향)

  • Han, Chang-Soo;Lee, Gyu-Seung;Lee, Moo-Sik;Hwang, Hye-Jeong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.12
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    • pp.3888-3894
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    • 2009
  • The purpose of this study was to compare the effect of changing the order of exercise types on body composition and blood lipid in obese women. The subjects were 40-years obese women. They were trained during the 12 weeks by combined exercise program which included aerobic and resistant exercises. 1. In the cases of weight, A group(aerobics and resistance training)more decreased than B group(resistance training and aerobics). There was not statistically significant difference before and after 12-week combined exercise program between the groups. In the case of body fat mass, A group more decreased than B group. There was a statistically significant difference between the groups(p<0.01). Lean body mass, A group more increased than B group but there was a statistically significant difference between the groups. A and B groups were decreased in abdominal percent fat and there was a not statistically significant difference between the groups. 2. There were not any significant differences between two groups after 12-week combined exercise program at total cholesterol(TC), triglyceride, and high density lipo-protein(HDL) cholesterol. 3. In the case of blood glucose, group A showed more decreased than group B after 12-week combined exercise program. The difference before and after exercise program of A and B groups was statistically significant(p<0.01). In conclusion, the combined training which include resistant and aerobic exercise may use selectively to induce change of body composition and blood lipid for weight loss in 40-aged obese wome

A study on Knowledge, Attitude and Practice about women who experienced induced abortion (인공임신 중절 경험 부인에 대한 지식, 태도 및 실천에 관한 조사연구)

  • 김상혜
    • Journal of Korean Academy of Nursing
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    • v.8 no.2
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    • pp.51-61
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    • 1978
  • Important object of this survey is for promoting of maternal health and popularizing a right recognition which analyzed and study about knowledge, Attituded and Practice about women who experienced induced abortion. This surveyed materials obtained from total 300 wome (each 150 cases) who experienced induced abortion at Obstetrics and Gynecological Department in K university hospital and D health center in Seoul for 1 month. (1978. 9. 1.-1978. 9. 30) Their Results are as follows: A. General characteristics of surveyed cases, The highest age distribution was between 30-34 (29.3%) in health center, upper than 40 (32.7%) in University hospital. B. Knowledge 1. Knowledge about the induced abortion obtained mainly from their friends in 36.4% and Hospital in 20.7%. 2. The rate of Women who have dangerous thinking about induced abortion was 63.3%. C. Attitude 1. The rate of women who gained guilt feeling after induced abortion was 52.7%, out of total 41.4% was responsed careless thinking if they have a proper reason. 2. Attitude about induced abortion when they have unwanted baby was absolutely necessary in 57.3%, relatively necessary in 30.6%, that is, most of women showed to want induced abortion if necessary. D. Practice 1. The women who have experienced induced abortion was only one times in 44.7%, two times in 22.0%, three times in 17.7%, total average times were 2.3. 2. The reason for induced abortion was highest due to limitation of family (38.0%), next failure of Contraception (15.8%). 3. The operating sites of induced abortion were local clinics in 93.0%, universal hospital in 7.0%. The difference was remakable. 4. The rate of complicated cases after induced abortion was 54.7%, among them, general weakness in 32.3%, vaginal bleeding or spotting in 23.8% and abdominal discomfortness or lumbago in 17.7%. When symptom appeared, the rate of treated Cases was 74.4%, their sites were general hospitals in 54.1%, local clincis in 18.0%, Herb medications in 15.6% and pharmaceutics in 12.3 in that order. 5. The average times of induced abortion follows by educational level were 2.6 in graduated elimentary school group, each 2.4 in graduated middle school and College group, 2.2 in graduated high school in that order. 6. The average times of induced abortion follows by ages were most with 3.3% in upper than 40 years old. The average times of all surveyed cases were 2.3.

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