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Isolation and Characterization of Three Kinds of Lipopeptides Produced by Bacillus subtilis JKK238 from Jeot-Kal of Korean Traditional Fermented Fishes (한국 전통젓갈에서 분리한 Bacillus subtilis JKK238 균주 유래 세 종류 Lipopeptide의 분리 및 특성)

  • Yoon Sang-Hong;Kim Jung-Bong;Lim Yoong-Ho;Hong Seong-Ryeul;Song Jae-Kyeung;Kim Sam-Sun;Kwon Soon-Wo;Park In-Cheol;Kim Soo-Jin;Yeo Yun-Soo;Koo Bon-Sung
    • Microbiology and Biotechnology Letters
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    • v.33 no.4
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    • pp.295-301
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    • 2005
  • About seven hundred bacterial strains were collected from Jeot-Kal, a Korean traditional fermented fishes, in various Korean districts. One of the strains designated JKK238 has its ability to antagonize in vitro the growth of a wide variety of plant pathogenic fungi responsible for diseases of economical importance. The JKK238 strain was isolated from Oh-Jeot, a kind of fermented shrimps, of Kangkyeung in Korea, and was identified as Bacillus subtilis based on its physiological characteristics, fatty acids compositions of cellular wall, and 16S rDNA sequence analysis. We isolated simply antimicrobial lipopeptides (AMLP) by $25\%$ ammonium sulfate precipitation of 3 days-old tryptic soy broth cultures of the JKK238 strain. Further analysis of AMLP revealed that B. subtilis JKK238 produces a wide variety of antifungal lipopeptide isomers from the iturin, fengycin and surfactin families simultaneously. Above results indicate that the JKK238 strain can be added to the limited number B. subtilis strains reported to co-produce the three kinds of lipopeptide families.

Synthesis of Poly(styrene-co-alkyl methacylate)s for Pour Point Depressants of Diesel containing Biodiesel (바이오디젤을 함유한 경유용 저온유동성 향상제의 합성: 폴리(스티렌-co-알킬 메타크릴레이트))

  • Yang, Young-Do;Kim, Young-Wun;Chung, Keun-Wo;Hwang, Do-Huak;Hong, Min-Hyeuk
    • Applied Chemistry for Engineering
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    • v.19 no.5
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    • pp.497-503
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    • 2008
  • A variety of techniques has been employed in order to reduce problems caused by the crystallization of paraffin and saturated fatty acid esters in diesel fuel containing biodiesels. Methacrylate copolymers are known as additives which reduce the pour point and cold filtering plugging point (CFPP) of diesel fuels. This paper describes the synthesis, characterization and low temperature properties, having as an initial step the synthesis of the alkyl methacrylate monomers by esterification of methacrylic acid with C12, C18, and C22 fatty alcohols. The copolymerization of these monomers with styrene was then performed, with molar ratios of 30:70, 50:50 and 70:30 for styrene:alkyl methacrylate. All copolymers were characterized by $^1H-NMR$, FT-IR, and gel permeation chromatography (GPC). The poly(styrene-co-alkyl methacrylate)s (PStmSMAn) leads to a large reduction in the pour point and CFPP of poly(styrene-co-alkyl methacrylate) in ultra low sulfur diesel (ULSD) and BD5 with treated 100~5000 ppm of poly(styrene-co-alkyl methacrylate). BD5 fuel containing 5000 ppm of the copolymer (PSt82SMA18) showed a $25^{\circ}C$ and $9^{\circ}C$ reduction in their pour points and CFPP, respectively.

An Exploratory Study on the Continuity of Consumer Empathy Response -Can Consumer Empathy Response expand on a Self-empathetic basis?- (소비자 공감반응의 연속성에 관한 연구 -소비자 공감반응은 자기 공감적으로 확장할 수 있는가?-)

  • Ock, Jung-Wo
    • Management & Information Systems Review
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    • v.39 no.1
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    • pp.75-91
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    • 2020
  • The purpose of this study is to examine the application of in-depth interviews in terms of exploration on the basis of the theoretical basis of various aspects of consumer empathy, and the question of whether the consumer can expand to the process of self empathy. Most studies related to consumer empathy divide the responsive dimension of consumer empathy into rational and emotional dimension based on empathy theory, and look at the empathic response perceived by consumers to the stimulus presented from the current point of view. In this study, however, we want to go one step further and confirm that the consumers themselves can ultimately go to the self-empathy stage by forming a creative street of reproduction and redevelopment. As a result of exploratory research through in-depth interviews, it was indirectly confirmed that consumer's empathetic response to specific marketing stimulus exists at the level of creative and self-empathetic as well as the emphasis of previous studies. Based on these findings, this study confirmed that consumer empathetic responses could go beyond a single dimension to form a multidimensional structure and move toward an expanded structure of empathic self-creation. This suggests that consumers' empathic responses should be grasped in terms of continuity of empathic responses rather than explained in a specific dimension. Although this study is meaningful as an early research of exploratory nature, it is necessary to supplement various content validity and refine the research method through subsequent studies. This study is expected to expand the understanding that consumer's empathy can be extended to other people's empathy and to be self-empathy.

Effects of Fermented Oyster Extract Supplementation on Body Composition, Muscular Strengths and Blood Muscle Growth Fact ors in Elderly Women (발효굴 섭취가 중년 여성의 신체조성, 근력 및 혈중 근성장 인자에 미치는 영향)

  • Park, Joung-Hyun;Kim, Dong-Seok;Lee, Bae-Jin;Her, Jung-Soo;Jeon, Byeong Hwan
    • Journal of Marine Bioscience and Biotechnology
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    • v.13 no.2
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    • pp.76-85
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    • 2021
  • Crassostrea gigas were fermented using L. brevis BJ20 to prepare fermented oyster extract (FO). The participants of this study were randomly assigned to FO and placebo (CON) groups. The FO group was given 1.0 g of FO supplementation and the CON group was given sucrose each day for eight weeks. The effects of FO supplementation on body composition, muscula r strength, and blood factors associated with muscle growth were assessed. The FO supplement was enriched with arginine (6,183.3 mg), phenylalanine (217.9 mg), leucine (122.6 mg), isoleucine (59.8 mg), valine (16.4 mg), and γ-amino butyric acid (GABA, 1,053.7 mg). The total fat was significantly decreased in the FO group compared with the CON group (p < 0.05). 60D/S Ext.T/Wo rk and 60D/S Flex.T/Work concomitantly with 60D/S Flex.PeakTQ/BW were significantly increase d by FO treatment compared to CON group (p < 0.05). However, posture stability was not significa ntly different between the groups. The levels of angiotensin-converting enzyme were significantly decreased within the FO group (p < 0.05). The FO group showed significantly decreased levels of tumor necrosis factor-α and increased levels of human growth hormone compared with the CON group (p < 0.01). The levels of insulin-like growth factor-1 increased (p < 0.01) in the FO group while that of creatine kinase and triglyceride decreased significantly compared with the CON group (p < 0.05). These results demonstrated that FO supplementation is effective in preventing sarcopenic obesity and maintaining and strengthening muscular function in elderly wom en. Hence, FO supplements can be used as functional ingredients for these benefits.

Analysis of Wikipedia Citations in Peer-Reviewed Journal Articles (학술논문에서의 위키피디아 인용에 관한 연구)

  • Shim, Wonsik;Byun, Jeayeon;Kim, Minjung
    • Journal of the Korean Society for Library and Information Science
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    • v.47 no.2
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    • pp.247-264
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    • 2013
  • Wikipedia represents a revolutionary experiment in the sense that it allows anonymous Internet users to contribute, change, and edit Encyclopedia contents used by tens of millions of people. While a very popular information source on the Internet, because of its questionable information credibility and accuracy, citing Wikipedia articles is being regarded as a risky behavior for scholars. The present study identified 282 scholarly articles from Thomson Reuters' Web of Science citation database that cite Wikipedia at least once. Out of the millions of articles indexed in Web of Science, the proportion of articles citing Wikipedia is extremely small. On the other hand, the numbers are showing a marked increase since 2011. Wikipedia citing articles are distributed in subject areas, such as library and information science, business, psychology, education, and communication more often than in other areas. The distribution of a total of 577 citations from 267 articles for which we were able to obtain full texts shows that Wikipedia is being cited mainly in studies of Wikipedia (139 citations, 24.1%) or as a ready reference source (331 citations, 57.4%). At the same time, about 15% of total citations turned out to be cases of potentially risky behaviors in which Wikipedia is being cited as a crucial basis or data source for study.

Identification of the Oligotrophic Bacteria Strain 7F Biocontrolling Phytophthora Blight Disease of Red-pepper (고추 역병 방제를 위한 저영양 길항세균 7F 균주의 동정)

  • Kim, Dong-Gwan;Yeo, Yun-Soo;Kwon, Soon-Wo;Jang, Kil-Su;Lee, Chang-Muk;Lee, Mi-Hye;Kim, Soo-Jin;Koo, Bon-Sung;Yoon, Sang-Hong
    • Research in Plant Disease
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    • v.16 no.1
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    • pp.41-47
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    • 2010
  • A total of 10,753 oligotrophic bacteria were isolated from the cultivated soils of red-pepper infected by Phytophthora blight disease in various regions of Korea (Chungju, Anmyon, Taean, Andong, Eumsung and Goesan). Seven bacteria isolates among these collected resources were selected by the first screening of in vitro antagonistic assay against major several plant pathogenic fungi including Phytophthora capsici. Finally, strain 7F was selected by pot assay for a possible biological control agent against Phytophthora blight disease of pepper seedling in the greenhouse. Strain 7F was identified as Bacillus subtilis on the basis of its 16S rDNA sequence analysis and as standardized biochemical characteristics assay kits such as API20 NE. In the experiment of P. capsici zoospore infected red-pepper on the pot test, infection rate of red-pepper with nonetreatment to Phytophthora blight disease was 87%, while the rate was only 6% in the pot treated with strain 7F. This result indicated that the Bacillus subtilis strain 7F will be useful as a potential biocontrol agent for Phytophthora blight disease of red-pepper.

Synthesis of Poly(alkyl methacrylate)s Containing Various Side Chains for Pour Point Depressants (서로 다른 측쇄 구조를 가진 폴리(알킬 메타크릴레이트)계의 저온유동성 향상제 합성)

  • Hong, Jin-Sook;Kim, Young-Wun;Chung, Keun-Wo;Jeong, Soo-Hwan
    • Applied Chemistry for Engineering
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    • v.21 no.5
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    • pp.542-547
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    • 2010
  • n-Paraffin and saturated fatty acid methyl esters in the diesel and bio-diesel fuel crystallize at low temperature. Many articles have addressed various solutions for the low temperature crystallization problem and one of them is the use of methacrylate copolymers. In this work, we synthesized a series of copolymers in the reaction condition of 70 : 30 molar ratio of lauryl methacrylate (LMA) (or stearyl methacrylate (SMA)) and alkyl methacrylates. The structures of the copolymers were characterized by $^1H$-NMR and FT-IR spectroscopy, and the molecular weight of copolymers were obtained from Gel Permeation Chromatography (GPC) method. The concentrations of additives were 500~1000 ppm and 1000~10000 ppm in diesel fuels and bio-diesel fuel (BD5 and BD20), respectively. The addition of copolymers changes the many properties of fuel such as the pour point (PP), cloud point (CP) and cold filtering plugging point (CFPP). For example, the low temperature properties of the copolymers containing SMA ($PSMAmR_2n$) were excellently improved about 15, 7, and $10^{\circ}C$ for PP, CP and CFPP, respectively.

Tracking Interdisciplinary Relationships between Scientific Researches in Energy Fields using Bibliometic Analysis (과학기술논문을 통한 에너지 연구 분야의 다학제 동태 추적분석)

  • Kang, Jong-Seok;Chung, Hyun-Sang;Lee, Il-Hyung
    • Journal of Korea Technology Innovation Society
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    • v.13 no.4
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    • pp.680-699
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    • 2010
  • In this paper, we attempted to analysis and track dynamic properties of the interdisciplinary relationship between scientific research areas in energy field using bibliometric analysis. We created network maps with SCs (subject categories) defined from $WoS^{\circledR}$ (Web of Science, Thomson Scientific ISI, Philadelphia, USA) using co-occurrence analysis method in order to identify overall disciplines directly linked with energy fields and investigate the change of interaction between SCs as a function of time. From the results of this study, thermodynamics, fuels, chemistry/chemical engineering, and electrochemistry have differentiated into more specific disciplines while the strength of interaction with energy field gradually has increased. Meanwhile, "nuclear physics" was developed to "nuclear science & technology" toward applicable target sector and also the interaction of "environmental science" with "energy generation area" among various energy disciplines recently showed the radical increase as compared with the values of 4 years ago. Finally, through combinative reviews of today's energy policy established by South Korea government, this study will give a help for keeping up with national energy agenda meeting the diverse characteristics of academic disciplines of energy field. In addition, our results support that the use of such network analysis based on bibliometric analysis to discern shifts in academic R&D strategies and target sectors.

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Analysis of Species Variety and Physiological Characteristics of Denitrifying Oligotrophic Bacteria Isolated from the Specific Environment in Korea (국내 특수 생태환경의 탈질 저영양 세균의 종 다양성 및 생리적 특성 분석)

  • Lee, Chang-Muk;Weon, Hang-Yeon;Kwon, Soon-Wo;Kang, Han-Chul;Koo, Bon-Sung;Yoon, Sang-Hong
    • Microbiology and Biotechnology Letters
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    • v.39 no.3
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    • pp.210-217
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    • 2011
  • In an effort to isolate novel bacteria for the bioremediation of over-fertilized soils, we identified 135 denitrifying cells out of 3,471 oligotrophic bacteria pools (3.9%) using a denitrification medium supplemented with potassium nitrate as the sole nitrogen source. Soil samples were taken from ecologically well-conserved areas, including a mountain swamp around the demilitarized zone (Yongneup), two ecoparks (Upo and the Mujechi bog), and ten representative islands around the Korean peninsula (Jejudo, Daecheongdo, Socheongdo, Baekryeongdo, Ulrungdo, Dokdo, Geomundo, Hongdo, Huksando and Yeonpyeongdo). All of the 135 bacteria produced nitrogen gas from the denitrification medium, and were proved to be nitrate reductase positive by API-BioLog tests. Phylogenetic analysis using 16S rDNA sequences revealed that the 135 bacteria consisted of 44 different genera. Along with the most prominent, Proteobacteria (87.4%), we identified denitrifying bacteria from Firmicutes (9.4%), Actinobacteria (2.4%), and Bacteroidetes (0.8%). Physiological analyses of the 44 representative denitrifying bacteria, under various pH levels, growth temperatures and salt stresses, revealed 12 favorable denitrifying strains for soil bioremediation.

Research Performance Evaluation Based on Quantitative Information Analysis in the Field of Herbal Medicine for Dementia Treatment (계량정보분석 기반의 연구개발 성과분석 : 치매 치료용 천연약물 분야)

  • Jeon, Won-Kyung;Han, Chang-Hyun;Kang, Jong-Seok;Heo, Eun-Jung;Han, Joong-Su;Lee, Young-Joon
    • Journal of Oriental Neuropsychiatry
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    • v.22 no.3
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    • pp.101-113
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    • 2011
  • Objectives : Trend of R&D of herbal medicine for dementia treatment was examined based on the quantitative information analysis for establishing the national strategy of research on dementia treatment with oriental medicine. Methods : Definition was made to clarify the technology for development of herbal medicine for dementia treatment. Based on the initial keyword provided by experts in the field, queries were compounded to conduct search in the search engines of WoS and DWPI. The raw data (papers or patents) extracted from the initial search were examined by expert-review before objects of analysis were determined. Then, the accumulated data was analyzed in terms of year, country and organization, which led to examination of the trend of R&D. And the research performance evaluation for dementia treatment technologies was also made in terms of country, organization and researcher based on the forward citation analysis. The international cooperation intensity was examined on the basis of analysis of network by researcher before analysis results were put together to select lead researchers. Results : According to the quantitative information analysis of 1,330 articles that were selected as analysis objects, the number of papers on natural products research for dementia treatment has increased by around 4.6 times in recent five years. This indicates that the intensive studies have been underway recently. It was found to be the US that had the highest level in research filed of herbal medicine for dementia treatment and the highest capacity of international cooperation for that purpose. On the contrary, Korea had the share of papers at 5.1%, the number of countries in cooperation research at 8, and the article quality index at 0.40, showing that the qualitative level was insufficient, compared to the quantitative outcome. In particular, Korea was found to have no intensity of international cooperation among researchers. In case of patent, the results of information analysis of 305 patents selected as analysis objects demonstrated that China had the highest share while Korea had the very low frequency of patent application quantitatively. Conclusions : In this study, the research to develop herbal medicine for dementia treatment has recently drawn much attention that has spread around the globe. Therefore, these results suggest establishing the strategy to develop technology for dementia treatment with oriental medicine in the future based on quantitative information analysis.