Browse > Article

Isolation and Characterization of Three Kinds of Lipopeptides Produced by Bacillus subtilis JKK238 from Jeot-Kal of Korean Traditional Fermented Fishes  

Yoon Sang-Hong (National Institute of Agricultural Biotechnology(MIAB))
Kim Jung-Bong (National Institute of Agricultural Biotechnology(NIAB))
Lim Yoong-Ho (Department of Molecular Biotechnolgy, Konkuk University)
Hong Seong-Ryeul (Department of Genetic Engineering, Sungkyunkwan University)
Song Jae-Kyeung (National Institute of Agricultural Biotechnology(NIAB))
Kim Sam-Sun (National Institute of Agricultural Biotechnology(NIAB))
Kwon Soon-Wo (National Institute of Agricultural Biotechnology(NIAB))
Park In-Cheol (National Institute of Agricultural Biotechnology(NIAB))
Kim Soo-Jin (National Institute of Agricultural Biotechnology(NIAB))
Yeo Yun-Soo (National Institute of Agricultural Biotechnology(NIAB))
Koo Bon-Sung (National Institute of Agricultural Biotechnology(NIAB))
Publication Information
Microbiology and Biotechnology Letters / v.33, no.4, 2005 , pp. 295-301 More about this Journal
Abstract
About seven hundred bacterial strains were collected from Jeot-Kal, a Korean traditional fermented fishes, in various Korean districts. One of the strains designated JKK238 has its ability to antagonize in vitro the growth of a wide variety of plant pathogenic fungi responsible for diseases of economical importance. The JKK238 strain was isolated from Oh-Jeot, a kind of fermented shrimps, of Kangkyeung in Korea, and was identified as Bacillus subtilis based on its physiological characteristics, fatty acids compositions of cellular wall, and 16S rDNA sequence analysis. We isolated simply antimicrobial lipopeptides (AMLP) by $25\%$ ammonium sulfate precipitation of 3 days-old tryptic soy broth cultures of the JKK238 strain. Further analysis of AMLP revealed that B. subtilis JKK238 produces a wide variety of antifungal lipopeptide isomers from the iturin, fengycin and surfactin families simultaneously. Above results indicate that the JKK238 strain can be added to the limited number B. subtilis strains reported to co-produce the three kinds of lipopeptide families.
Keywords
Bacillus subtilis; lipopeptides; antifungal activities; iturin; fengycin; surfactin;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Grau, A., C. Juan, J. C. Gomez-Fernandez, F. Peypoux, and A. Ortiz. 2001. Aggregational behavior of aqueous dispersions of the antifungal lipopeptide iturin A. Peptides 22: 1-5   DOI   ScienceOn
2 Hiraoka, H. O. Asaka, T. Ano, and M. Shoda. 1992. Characterization of Bacillus subtilis RB 14, Coproducer of peptide antibiotics iturin A and surfactin. J. Gen. Appl. Microbiol. 38: 635-640   DOI   ScienceOn
3 Kluge, B., J. Vater, J. Salnikow, and K. Eckart. 1988. Studies on the biosynthesis of surfactin, a lipopeptide antibiotic from Bacillus subtilis ATCC 21332. FEBS Left. 231: 889-901
4 Lin, S. C. and H. J. Jiang. 1997. Recovery and purification of the lipopeptide biosurfactant of Bacillus subtilis by ultrafiltration. Biotechnol. Techniq. 11: 413-416   DOI   ScienceOn
5 Roongsawang, T., T. Kameyama, M. Haruki, M. Morikawa. 2002. Isolation and characterization of a halotolerant Bacillus subtilis BBK-l which produces three kinds of lipopeptides: bacillomycin L, plipastatin, and surfactin. Extremophiles 6: 499-506   DOI   ScienceOn
6 Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. lmmunol. Methods 65: 55-63   DOI   PUBMED   ScienceOn
7 Wang, J., J. Liu, X. Wang, J. Yao, and Z. Yu. 2004. Application of electrospray ionization mass spectrometry in rapid typing of fengycin homologues produced by Bacillus subtilis. Lett. Appl. Microbiol. 39: 98-102   DOI   ScienceOn
8 Phister, T. G., D. J. O'Sullivan, and L. L. McKay. 2004, Identification of bacilysin, chlorotetaine, and iturin A produced by Bacillus sp. strain CS93 isolated from Pozol, a Mexican fermented maize dough. Appl. Environ. Microbiol. 70: 631-634   DOI   ScienceOn
9 Razafindralambo, H., and M. Paquot. 1993. Purification of antifungal lipopeptides by reversed-phase high-performance liquid chromatography. J. Chromatography 639: 81-85   DOI   ScienceOn
10 Tsuge, K., T. Ano, and M. Shoda. 1996. Isolation of a gene essential for biosynthesis of the lipopeptide antibiotics plipastatin B1 and surf actin in Bacillus subtilis YB8. Arch. Microbiol. 165: 243-251   DOI   ScienceOn
11 Regine, M.D. and F. Peypoux. 1994. Iturins, a special class of pore-forming lipopeptides: biological and physicochemical properties. Toxicology 87: 151-174   DOI   PUBMED   ScienceOn
12 Akpa, E., P. Jacques, B. Wathelet, M. Paquot, R. Fuchs, H. Budzikiewicz, and P. Thonart. 2001. Influence of culture conditions on lipopeptide production by Bacillus subtilis. Appl. Biochem. Biotechnol. 91: 551-561   DOI   ScienceOn
13 Vanittanakam, N. and W. Loeffler. 1986. Fengycin - a novel antifungal lipopeptide antibiotics produced by Bacillus subtilis F29-3. J. Antibiotics (Tokyo) 39: 888-901   DOI