• The Korean Journal of Mycology
• /
• v.46 no.3
• /
• pp.307-314
• /
• 2018

The goal of this study was to investigate the microbiological characteristics of the ethanol-producing wild yeast, Aureobasidium pullulans P-1, isolated from flowers growing near the Yedang reservoir, Chungnam province, Korea, and in addition, to optimize its fermentation ability for the production of Makgeolli. A. pullulans P-1 was oval-shaped, and formed ascospores and pseudomycelium. The P-1 strain was a halophilic and sugar tolerant yeast which grew in 15% NaCl and 50% glucose-containing yeast extract-peptone-dextrose media. The P-1 strain was also resistant to 20% ethanol. Changes of the physicochemical properties during Makgeolli fermentation by A. pullulans P-1 were investigated. A maximum of 8.45% ethanol was obtained when a mixture of cooked rice, 150% water, and 35% ipguk per cooked rice was fermented by 5% A. pullulans P-1 culture broth at $25^{\circ}C$ for 10 days. Antihypertensive angiotensin I-converting enzyme inhibitory activity in the Makgeolli ferment produced by A. pullulans P-1 reached a high of 71.1% after 10 days.

• Horticultural Science & Technology
• /
• v.31 no.4
• /
• pp.467-472
• /
• 2013

Twenty and 16 accessions of the countrywide weedy melon (Cucumis melo var. agrestis) collections were evaluated in 1986 and 1990, respectively. There was a good variation in the plant height and their leaves were small in general. In sex expression, 30 of the accessions were andromonoecious, 4 were monoecious and 2 were hermaphrodite. Female flower set on main stems was extremely rare except for on hermaphrodite. On andromonoecious and monoecious plants, abundant female flowers set at the 1st and 2nd node of branch vines. Bitter taste was found in the young fruits of all the accessions tested. Mature fruits were dark yellow, yellow, light yellow or milky white in color, small in size, and very low in sweet taste. The number of seeds per fruit varied from 50.6 to 158.4 showing the characteristics of wild species for perpetuation in wild.

• Korean Journal of Environmental Agriculture
• /
• v.34 no.2
• /
• pp.149-154
• /
• 2015

BACKGROUND: Yeast isolates associated with the leaves, stems, and flowers of the tiger lily needed to be identified using isolation methods that have previously been used effectively in yeast biotechnology. A culture-based approach was necessary for the isolation of many yeast strains associated with tiger lily. METHODS AND RESULTS: In this study, the homogenized leaves, stems, and flowers of tiger lily were spreaded onto GPY medium containing chloramphenicol, streptomycin, Triton X-100, and L-sorbose. A total of 82 yeast strains from the leaves, 94 and 97 yeast strains from the stems and flowers were isolated, respectively. Yeast isolates were identified by phylogenetic analysis based on internal transcribed spacer region sequencing. The yeast species isolated from the leaves comprised of 31 isolates of the genus Pseudozyma, 28 of Aureobasidium pullulans, and 11 of the genus Cryptococcus. Those isolated from the stems comprised of 40 of A. pullulans and 11 of Cryptococcus, and 95 of A. pullulans While, 1 isolate each of the genera Rhodotorula and Metschnikowia were isolated from the flowers. CONCLUSION: We identified site-specific yeast communities associated with tiger lily. These yeast isolates may have high potential for application in the field of biotechnology.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.28 no.11
• /
• pp.1353-1360
• /
• 2004

The purpose of this study is to develop a cultural products design of korean image using fabrics. The study is carry out in literature research methods and make sample product. We examine existing researches about cultural product design development to understand developed items, materials, and design directions. The brainstorming method and the established literature researches were used to obtain the design components of Korean image. A cultural products design was developed into the decorative structural details. The results can be summarized as follows: Korean image is pure, passive, gentle, small, and lasting life-force at weeds and wild flowers. Korean form is rounded curve and soft silhouette as streamy, not exaggerate. Korean color is not a finishing treatment, non-dyed natural color, or pastel, light greyish, and dull tone. Korean textile is a natural materials as linen, ramie, and coarse silk. Final sample products are table supplies, home goods and bed goods applying the sewing method of tuck, quilting, top-stitching, patch work, applique and embroidery.

• Journal of Life Science
• /
• v.11 no.3
• /
• pp.242-247
• /
• 2001

Botrytis cinerea E12 was isolated from the leaves, flowers and fruits of eggplant in the greenhouse in Halrim, Kimhae and Dejeo, Pusan. The leaves infected with the pathogen were appeared initially brown-color, small gray spots at the edge, and finally fall down. The fruit was showed the symptoms of circular or irregular shapes, followed by sunken. When the symptoms were developed, the conidia formed on the surface with gray color. To determine the pathogenicity of B. cinerea E12 against the eggplants, the conidia were suspended with 30% tomato juice, PDB and sterile water, respectively. The result showed that the conidial suspension with 30% tomato juice was highly effective on the pathogenicity as more than 90%. Moreover, the symptoms caused by inoculum were the same as those of wild-type pathogen.

• Journal of Agricultural Extension & Community Development
• /
• v.10 no.2
• /
• pp.251-266
• /
• 2003

Diffusion of non-professional flower growing in Korea still lags behind. The objectives of the study are to establish a theoretical modes to explain the diffusion of social floriculture, to identify some characteristics and problems of growing flower crops to cultivate amenities and emotions, and to suggest remedial measures for fostering it. Theoretical model adopted in this study consists of such variables as education, social movement, policies, diffusion of social flower growing, amenities, and emotional cultivation. For identifying the diffusion characteristics of flower growing, projects implemented by a non-governmental organization, schools, local and central government organizations were analyzed. In order to stimulate the diffusion of social flower growing and to increase its effects, there needs to strengthen education of stakeholders, establishment of partnership among schools, non-governmental and governmental organizations, and institutional supports including manpower and finances. Linking flower growing projects to community festival and tourism development programs can foster institutionalization of community flower growing. Furthermore, the introduction of wild, symbolic and traditional flowers and trees will help to specialize and improve community landscapes.

• Korean Journal of Agricultural Science
• /
• v.46 no.2
• /
• pp.335-340
• /
• 2019

Two species of Leycesteria, L. gracilis (Kurz) Airy Shaw and L. formosa Wall. (Caprifoliaceae), from the Lao Cai and Yen Bai Provinces are reported here as a new generic record for the flora of Vietnam. During floristic surveys of northern Vietnam, L. gracilis and L. Formosa were encountered. A critical morphological study of all the collected specimens and the type of materials consulting the relevant literature led us to confirm the identity of our collected specimens as L. gracilis and L. formosa. The genus differs from other Vietnamese genera of Caprifoliaceae by the presence of a spike inflorescence or flowers in sessile whorls of 6. Taxonomic notes, description, and photographs are provided, together with short notes on the distribution, ecology and phenology of the two species.

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2003.04a
• /
• pp.49-55
• /
• 2003

The morphology of the leaves and the flowers of angiosperms exhibit remarkable diversity. One of the factors showing the greatest variability of leaf organs is the leaf index, namely, the ratio of leaf length to leaf width. In some cases, different varieties of a single species or closely related species can be distinguished by differences in leaf index. To some extent, the leaf index reflects the morphological adaptation of leaves to a particular environment. In addition, the growth of leaf organs is dependent on the extent of the expansion of leaf cells and on cell proliferation in the cellular level. The rates of the division and enlargement of leaf cells at each stage contribute to the final shape of the leaf, and play important roles throughout leaf development. Thus, the control of leaf shape is related to the control of the shape of cells and the size of cells within the leaf. The shape of flower also reflects the shape of leaf, since floral organs are thought to be a derivative of leaf organs. No good tools have been available for studies of the mechanisms that underlie such biodiversity. However, we have recently obtained some information about molecular mechanisms of leaf morphogenesis as a result of studies of leaves of the model plant, Arabidopsis thaliana. For example, the ANGUSTIFOLIA (AN) gene, a homolog of animal CtBP genes, controls leaf width. AN appears to regulate the polar elongation of leaf cells via control of the arrangement of cortical microtubules. By contrast, the ROTUNDIFOLIA3 (ROT3) gene controls leaf length via the biosynthesis of steroid(s). We provide here an overview of the biodiversity exhibited by the leaf index of angiosperms. Taken together, we can discuss on the possibility of the control of the shapes and size of plant organs by transgenic approaches with the results from basic researches. For example, transgenic plants that overexpressed a wild-type ROT3 gene had longer leaves than parent plants, without any changes in leaf width. Thus, The genes for leaf growth and development, such as ROT3 gene, should be useful tools for the biodesign of plant organs.

• Journal of Species Research
• /
• v.4 no.2
• /
• pp.152-158
• /
• 2015

Dendranthema boreale and D. indicum are easily distinguished from other Korean Dendranthema spp. by having yellow flowers. We have found a putative new taxon of Dendranthema having white flowers, except for sharing most characters with Dendranthema boreale. The chloroplast (cp) genome of the putative new taxon of Dendranthema, Dendranthema sp. K247003, registered in National Agro-Biodiversity Center (ABC), was completely characterized as a genetic barcode. The cp-genome of Dendranthema sp. K247003 was 151,175-bp in size: LSC was 82,886-bp, IR 24,971-bp, SSC 18,347-bp. The cp-genome of Dendranthema sp. K247003 contains 113 genes and 21 introns consisted of 79 protein coding genes, 4 RNA genes, and 30 tRNA genes, with 20 group II introns and one group I intron. Some of the genes and there introns were duplicated in IR. The cp-DNA of Dendranthema sp. K247003 is distinguished from that of D. boreale IT121002 by 67 SNPs in genic regions of 24 protein coding genes and by a 9-bp INDEL in ycf1. Further cp-DNA study will give us better information on genetic markers of Dendranthema species.

• Journal of Plant Biotechnology
• /
• v.38 no.1
• /
• pp.54-61
• /
• 2011

Antisense construct of cDNA for senescencerelated ACC oxidase (CAO) cDNA isolated from carnation flowers were introduced into tobacco by Agrobacteriummediated transformation. The decreasing expression of NtACO and the reduction of ethylene production were observed in these transgenic lines. In contrast, the SAMDC transcripts and spermidine content were increased. The findings that higher content of spermidine in the ethylene suppressed transgenic plants compared with wild-type should be directly resulted in the enhancement of SAMDC activity followed by the increased accumulation of SAMDC transcript. To investigate the pathogenic response in these transgenic plants, wild-type and transgenic plants were inoculated with Phytophthora parasitica pv. nicotianae. Transgenic plants suppressing ethylene production showed the increased resistance against fungal pathogen, comparing with wild-type plant. PR-protein genes expression in CAO-AS-2 and CAOAS-4 were also higher at the normal growth condition and pathogenic response than in wild-type plants. The results of higher spermidine content and SAMDC activity in transgenic plants, CAO-AS-2 and CAO-AS-4, support the possibility that an increase in spermidine content might induce the higher transcripts of PR-protein genes. This results agreed with the phenomena that spermidine promoted the expression of PR1a and a SAMDC inhibitor, MGBG, decreased the expression of PR1a in leaf discs. These results suggest that the resistance against fungal pathogen in transgenic tobacco impaired in ethylene production might be caused by increasing in polyamine, especially spermidine, biosynthesis.