• The Korean Journal of Mycology
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• v.22 no.2
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• pp.172-183
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• 1994

The productions of arbuscular mycorrhizal fungal(AMF) spores were observed by adding three different commercial fertilizers on AMF inhabiting soils. Various morphological features, vesicles, arbuscles, sporulations of spore, and flower-like-structures, were also found in the mycorrhizal roots during 80 days after transplanting. Spore prodcutions of the employed AMF were observed to be periodically increased with the intervals of 40 days. Sorghum, green onion, hot pepper, and wild legume plants were appeared to be a good plant for productions of AMF and as the host of AMF. The productions of AMF spores was inversely related to phosphate fertilizer, and also observed to be low in the plant pots added with whole balanced fertilizers.

• Korean Journal of Veterinary Service
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• v.30 no.1
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• pp.23-32
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• 2007

A total of 60 samples (38 domestic honeys and 22 foreign honeys) were collected from October in 2003 to May in 2004 in Incheon metropolitan area, and contents of important quality-related components in honeys were analyzed using methods of Korea Food Code. Most of the honeys were satisfied with Korean limit (21%) in moisture content even though 4 samples brought by Korean travellers and 1 sample in domestic honey (wild flower) were higher than the limit. The lowest mean level of moisture in the honeys was imported foreign honeys (16.95%). All of the samples were below of 0.6% in the analysis of ash contents. The highest content was imported foreign honey (0.19%) while the lowest content was in domestic acacia honey (0.05%). Acidity of domestic honeys (9.49-9.94meq/kg) was approximately half of that in foreign honeys. All samples were satisfied with Korean limit (40.0meq/kg). In the analysis of fructose and glucose, only three samples of foreign honeys were lower than Korean limit (sum of both ; 65%). The content of sucrose in all samples was less than 7%. Mean of Hydroxymethylfurfural (HMF) contents were 12.83-24.3mg/kg in domestic honey, and 31.34-45.58mg/kg in foreign honey. However, three samples brought by Korean travellers were not satisfied with Korean limit (${\leq}80mg/kg$). In conclusion, quality of domestic honey was better than that of foreign honey. Also, it may be needed that the honeys purchased by Korean travellers in foreign countries should be continuously monitored.

• Journal of Plant Biotechnology
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• v.31 no.2
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• pp.121-126
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• 2004

Bleeding heart (Dicentra spectabilis L. Lemaire) is one of the most valuable wild flower in Korea. This work was conducted for the mass production of somatic embryos through suspension culture and more effective plant regeneration system in Dicentra spectabilis. High-frequency embryogenic callus proliferation was achieved in SH liquid medium supplemented with 1 mg/L 2,4-D. Half-strength SH medium was suitable concentration for somatic embryo induction and germination. About 5,000 embryos were produced per 250$m\ell$ flask after 4 weeks of culture. Germination rate of somatic embryos was decreased when GA$_3$ was added in medium. The plantlets showed a 58％ survival rate when transferred to pots after 1 month of culture. The results indicate that micropropagation procedure via somatic embryogenesis can be applied for an efficient mass propagation of Dicentra spectabilis.

• Journal of Plant Biotechnology
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• v.37 no.1
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• pp.89-95
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• 2010

Dihydroflavonol 4-reductase (DFR) is a key enzyme of the flavonoid biosynthesis pathway which catalyses the NADPH-dependent reduction of 2R,3R-trans-dihydroflavonols to leucoanthocyanidins. In this study we describe cloning and expression of the genes encoding the flavonoid-biosynthetic enzyme DFR in Gypsophila paniculata L. Inspection of the 1279 bp long sequence revealed an open reading frame 1063 bp, including a 36 bp 5' leader region and 181 bp 3' untranslated region. Comparison of the coding region of this DFR cDNA sequence including the sequences of Arabidopsis thaliana, Citrus sinensis, Dianthus caryophyllus, Ipomoea batatas, Matthiola incana, Nierembergia sp, Petunia hybrida, Solanum tuberosum, Vitis vinifera reveals an identity higher than 69% at the nucleotide level. The function of this nucleotide sequences was verified by comparison with amino acid sequences of the amino-terminus and tryptic peptides from purified plant enzyme, by northern blotting with mRNA from wild type and mutant plants, by in vitro expression yielding and enzymatically active reductase, as indicated by the small leucopelargonidin peak. Genomic southern blot analysis showed the presence of only one gene for DFR in Gypsophila paniculata.

• Korean Journal of Veterinary Service
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• v.28 no.3
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• pp.203-213
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• 2005

Oxytetracycline, tetracycline, chlortetracycline and doxycycline in honey were separated by solid phase extraction (SPE) and determined with high performance liquid chromatography (HPLC) with UV/Visible detector. Analysis was carried out using following conditions: XTerra $C_8$ column $(3.9\times150mm\;i.d. 5{\mu}m)$, mobile phase composed of 0.01M oxalic acid : methanol : acetonitrile (820 : 80 : 100, v/v/v), isocratic pump at a flow rate of 0.9 ml/min. and $50{\mu}l$ of injection volume, UV/Visible detector with wavelength of 360nm. The calibration curves of four tetracyclines showed linearity $(\gamma^2>0.999)$ at concentration range of $100\~1,000 ng/ml$. The recoveries in fortified honey represented more than $70\%$ with low coefficient of variation $(<10\%)$ for concentration range of four tetracyclines. The detection limits for oxytetracycline, tetracycline, chlortetracycline and doxycycline were 13.8, 14.6, 26.2 and 24.9ng/g in acacia honey. respectively. We also monitored tetracyclines residue in domestic honey [n : 38, acacia (20), wild flower (18) ] and foreign honey [n=22, legally distributed (13), illegally distributed (9)] using modified Charm II screening and HPLC confirmation methods. Seven of the 60 samples $(11.7\%)$ were suspect positive using modified Charm II screening test. Chlortetracycline residue was found in one foreign honey (illegally distributed) tested at concentrations of 0.22 ppm. Conclusively, for more effective control of tetracyclines used in beekeeping should be further survey for residues in honey and also national guidelines (maximum residue limit : MRL) and methods should be obligatory.

• Korean Journal of Veterinary Service
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• v.29 no.4
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• pp.451-457
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• 2006

This study was conducted to investigate the contents of lead (Pb) and cadmium(Cd) in honeys (n=60) from April to May in 2004 and to provide the scientific basis for heavy metal regulations of Korea Food Code. The samples were digested with acids, then analyzed for the contents of Pb and Cd by an inductively coupled plasma spectrometer (ICP). The contents of Pb and Cd [minimum-maximum (mean)] were 0.189-1.82 mg/kg (0.568) and not detected (ND)-0.016 mg/kg (0.0008) in domestic acacia honeys (n=20), ND-1.702 mg/kg (0.329) and ND-0.243 mg/kg (0.013) in domestic wild flower honeys (n= 18), ND-0.322 mg/kg (0.073), ND-0.027 mg/kg (0.002) in imported honeys (n=13), ND-3.754 mg/kg (0.671) and ND-0.658 mg/kg (0.073) in foreign honeys (n=9) brought by Korean travellers, respectively. According to the results, foreign honeys brought by Korean travellers were detected with the highest level of Pb and Cd. Therefore, we recommend that heavy metals of domestic and foreign honeys should be continuously monitored. It is also thought that these results could be the important references to establish the standard of Pb and Cd in honey.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.57-63
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• 2005

In order to develop the mass production method of anticancer compound-polyacetylene from tissue culture of Panax ginseng C.A. Mayor, these studies were carried out for the effects of various chemicals used as precursor and elicitor in viかo. Ginseng callus cultured on the growth medium containing 5mg/l L-phenylalanine was well grown and detected polyacetylene compounds as well as panaxydol and panxynol. But same media containing $\alpha-methyl-D.L.methionine$ and D.L.-norleucine was not detected any polyacetylene. Panaxydol, one of polyacetylene and active anticancer compound, was detected in calli cultured on media with upper 1mg/l chitosan used as elicitor, but panaxynol was not detected. Nigeran used as active elicitor, caused to decrease the growth of ginseng callus, and don't work as elictor on the biosynthesis of polyacetylene from ginseng callus.

• Journal of Life Science
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• v.16 no.7 s.80
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• pp.1195-1198
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• 2006

The importance of wild plant resources along with the development of high biotecyhnology is highlighted for exploitation of new materials which can make the added value. The goal of this study is providing fundamental data bases for developing new materials through the analyses of morphological characteristics, antibiotics and molecular cytogenetics in Baegseon (Dictamnus dasycarpus Turcz.). Baegseon has several characteristics; there are two types of flower color, pink and white, the seed germination starts about February 20th, the maximum flowering season is around May 17th in southern Korea, the growth period is about 60 days. The number of chromosomes are 2n=2x=36, the size of chromosomes in metaphase is $4.2{\sim}8.1{\mu}m$. The amount of 2C nuclear DNA is 1.93pg, and there is no variation of genome size amoung varieties. The extraction juice of baegseon young roots has the excellent antibiotic activity against the mold (Botrytis cinerea).

• Journal of the Korea Fashion and Costume Design Association
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• v.16 no.2
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• pp.41-53
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• 2014

Contemporary fashion designers are keen on linking the past and the present through culture and fashion by the attempt to investigate and research on the costumes recorded in its history and to reinterpret it on their own terms. Covering the history of culture ranging from the 70s and 80s to the ancient Greek and Egyptian periods, these reinterpretations also reflect the latest fad of the times in which a fashion designer lives and works. Above all, Rococo costume from the eighteenth century is one of the fashion styles adored by fashion designers. What constitute the costume of this period creating the image of sensual women are not only the form of outfit, colors, the design of accessories, shoes and hairstyle but also the details such as frills, ribbons and flower decorations. In contemporary times, the original Rococo style has been modified and turned into various styles in the haute couture and street fashion. These modernized styles include Rock Rococo, Modern Rococo, Lolita Look, Gyaru Fashion and etc., all of which adopt the Rococo style and consider it as the symbol of feminine beauty. However, their approaches to the original Rococo style differ from each other. Hence, advocating the elegance of women is not invariably the case. Often, the interpretations of the Rococo are unconventional, defiant and wild. And, sometimes, these new versions of Rococo are overly exaggerated and emphasized or, reversely, reduced. In this research, various references on the Rococo elements such as colors, forms, accessories and detailed features that has influenced on the contemporary fashion will be analyzed. This analysis will demonstrate how the Rococo style of the eighteenth century has been integrated into contemporary fashion giving birth to new Rococo fashion styles.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.23 no.1
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• pp.97-106
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• 2020

Urban parks provide a variety of ecosystem services and are an important means of providing positive functions to urban ecosystems. Recently, various studies on wildlifes in urban parks have been conducted. However, there is a lack of research on habitat use in urban parks at important times such as before and after fledging in bird ecology. This study analyzed habitat use and home-range before and after fledging on Cheongsa park, a neighborhood park located in Cheonan city. An artificial nest was set up to check and capture great tit in fledging time. One female was captured and attached to the NTQB-2 (0.4g) radio transmitter, the location was tracked using SIKA Radio Tracking Receiver, hand-held three element Yagi antenna and GPS. Location information was recorded for 10 minutes for 3 hours each morning and afternoon for 12 days from May 17 to May 31, 2019. As a result, the home-range of the target species was 1.776 ha (MCP) and the core area was 499 ㎡ (KD 50%). The average daily home-range was 0.513 ha for the entire period, 0.688 ha before fledging, 0.339 ha after fledging based on MCP. The bird moved about 29.9 m on average and moved up to 131.7 m. For the most of the time, the great tit stayed inside the park, but the bird also used small green spaces such as street trees, tree flower beds, and green areas of unused lands. The results of this study could be applied to the study of habitat use and the greenery management policy of the urban park considering wild birds.