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The Effects of Various Chemicals on the Production of Polyacetylene in Ginseng Callus in vitro Culture  

Yoon Jae-Ho (Institute of KuRae Wild Flower)
Song Won-Seob (College of Agriculture and Life Sciences, Sunchon National University)
Lee Mee Sook (Dept. of Food and Nutrition, Hannam University)
Yang Deok Chun (College of Life Science, Kyung Hee University)
Publication Information
Korean Journal of Plant Resources / v.18, no.1, 2005 , pp. 57-63 More about this Journal
Abstract
In order to develop the mass production method of anticancer compound-polyacetylene from tissue culture of Panax ginseng C.A. Mayor, these studies were carried out for the effects of various chemicals used as precursor and elicitor in viかo. Ginseng callus cultured on the growth medium containing 5mg/l L-phenylalanine was well grown and detected polyacetylene compounds as well as panaxydol and panxynol. But same media containing $\alpha-methyl-D.L.methionine$ and D.L.-norleucine was not detected any polyacetylene. Panaxydol, one of polyacetylene and active anticancer compound, was detected in calli cultured on media with upper 1mg/l chitosan used as elicitor, but panaxynol was not detected. Nigeran used as active elicitor, caused to decrease the growth of ginseng callus, and don't work as elictor on the biosynthesis of polyacetylene from ginseng callus.
Keywords
anticancer; callus culture; chitosan; L-phenylalanine; polyacetylene; panaxydol;
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