• Title/Summary/Keyword: White-Cell

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Biocompatibility of bioaggregate cement on human pulp and periodontal ligament (PDL) derived cells (사람의 치수 및 치주인대 세포에 대한 Bioaggregate 시멘트의 생체적합성에 대한 연구)

  • Chung, Choo-Ryung;Kim, Eui-Seong;Shin, Su-Jung
    • Restorative Dentistry and Endodontics
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    • v.35 no.6
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    • pp.473-478
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    • 2010
  • Objectives: This study was performed to investigate the biocompatibility of newly introduced Bioaggregate on human pulp and PDL cells. Materials and Methods: Cells were collected from human pulp and PDL tissue of extracted premolars. Cell culture plate was coated either with Bioaggregate or white MTA, then the same number of cells were poured to cell culture dishes. Cell attachment and growth was examined under a phase microscope after 1,3 and 7 days of seeding. Cell viability was measured and the data was analyzed using Student t-test and one way ANOVA. Results: Both types of cells used in this study were well attached and grew healthy on Bioaggregate and MTA coated culture dishes. No cell inhibition zone was observed in Bioaggregate group. There was no statistical difference of viable cells between bioaggreagte and MTA groups. Conclusions: Bioaggregate appeared to be biocompatible compared with white MTA on human pulp and PDL cells.

Morphological Characteristics and Composition of Cell Wall Polysaccharides of Brassica campestris var. pekinensis (Baechu) (배추조직의 형태학적 특성과 세포벽 다당류의 조성)

  • Kim, Sun-Dong;Park, Hong-Deok;Kim, Mi-Gyeong
    • Food Science and Preservation
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    • v.4 no.3
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    • pp.301-309
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    • 1997
  • This study was conducted to examine morphological characteristics and the content of cell wall polysaccharides of Brassica campestris var. pekinensis(baechu). First of all, the variety of scientific name and naming of parts of baechu in the literatures of kimchi showed, which will unify marks. So, we propose not so much mid-rib and leaf blade of baechu leaf as white part and green part, respectively. On the other hand, the forms of vessel elements of white part in baechu consist in ring, sclariform and reticulate thickening. The proximate compositions and contents of cell wall polysaccharides of baechu has significant differences between its cultivars. The cell wall pectin from baechu exhibited four peals with molecular weights of 2,000,000, about 100,000 and less than 10,000 by gel filteration chromatography and hemicellulose did two peaks with molecular weights of 2,000,000 and 10,000.

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Authentic-color Characteristic of the Fringe-field Switching Mode using a Liquid Crystal with Negative Dielectric Anisotropy (유전율 이방성이 음인 액정을 이용한 Fringe-field Switching Mode의 Authentic-color 특성)

  • Song, Je-Hoon;Choi, Yoon-Seok;Moon, Dae-Gyu;Han, Jeong-In;Lee, Seung-Hee
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.17 no.6
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    • pp.633-640
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    • 2004
  • We have studied color tracking of a fringe-field driven homogenously aligned nematic liquid crystal (LC) cell with negative dielectric anisotropy and compared it with other devices such as the twisted nematic(TN) and in-plane switching(IPS) modes. According to studies, the TN device shows bluish color at grey scale and even at a low retardation cell it cannot avoid color tracking. The authentic IPS device having cell retardation value of 0.23 ${\mu}{\textrm}{m}$ also shows bluish white color. However, the FFS device shows excellent color tracking characteristics even at high retardation value of the cell while keeping high transmittance and greenish white.

Anti-Helicobacter pylori, Cytotoxic, and Anti-inflammatory Activities of White Ginseng Extract

  • Jee, Hee-Sook;Chang, Kyung-Hoon;Moon, Sun-Hee;Park, Se-Ho;Paik, Hyun-Dong
    • Food Science and Biotechnology
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    • v.17 no.5
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    • pp.1106-1109
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    • 2008
  • The anti-Helicobacter pylori activity, cytotoxicity, and anti-inflammatory activity of white ginseng extract (WGE) were investigated in vitro in this study. The antimicrobial effects of WGE toward H. pylori strains 52 J99, SSI, and 51 were tested using the disk diffusion method. Among these H. pylori strains, H. pylori 52 was the most sensitive, having the largest inhibition zone (19 mm), followed by J99, SSI, and 51. The zone of inhibition due to WGE increased significantly with increasing dosage. The cytotoxicity of WGE toward the human cancer cell lines A-549 (human lung carcinoma), HEC-1-B (human endometrial adenocarcinoma), HeLa (human uterin adenocarcinoma), and SW-156 (human kidney carcinoma) was measured using the 3-(4,5-dimethylthizol-2-yl)-2,5-diphenylate-tetrazolium bromide (MTT) assay. WGE exhibited an inhibitory effect on cell growth at 2.0 mg/mL for all tumor cell lines. An analysis of anti-inflammatory activity using the RAW 264.7 cell line showed that the inhibition of nitric oxide (NO) production increased as the WGE content increased. These results demonstrate the potential of WGE to be used as a health-promoting substance.

Hematologic and Coagulation Changes in Hypothermic Dogs (신체냉각이 혈액세포성분 및 응혈기전에 미치는 영향에 관한 실험적 연구)

  • 최대영
    • Journal of Chest Surgery
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    • v.2 no.1
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    • pp.105-114
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    • 1969
  • This experiment was carried out to study the responses of cellular component of blood and bone marrow to cold and also the changes of coagulation during cooling. Forty-two mongrel dogs were subjected to hypothermia by ice-water surface cooling technique. Lowest body temperature ranged from 21-23 degree. Dogs were divided into 3 groups,Group I, 12 dogs: pentothal anesthesia for 3 hours, Group II, 20 dogs;hypothermic group and Group III,10 dogs;postsplenectomy hypothermic group. Results were summarized as follows: 1. Hemoglobin, hematocrit and red blood cell count significantly increased when animals were cooled, and increase was noted in similar magnitude among the animals of Group I. 2. White blood cell count extremely decreased after cooling and effect of splenectomy on white blood cell count was not apparent. No significant changes were seen among Group I. 3. Differential count of white blood cell when cooled showed relative increase of polymorphonuclear neutrophil and decrease of lymphocyte. 4. There was marked decrease of platelets when body temperature reached to 21-23degree and essentially. no changes was noted in Group I. 5. Clotting time, bleeding time, plasma prothrombin time, recalcification time, and fibrinolysis showed no significant changes when dogs were cooled. Clot retration and prothrombin consumption during hypothermia appeared to be poor. In Group II, bleeding time decreased after splenctomy and when body temperature was lowered, plasma prothrombin time, clot retraction, and prothrombin consumption decreased. Decreased bleeding time and poor clot retraction were noted in Group I. 6. It was found that megacaryocyte count decreased even though platelet count of peripheral blood markedly diminsished when animals were cooled. There was some tendency of erythroid hyperplasia noted during hypothermia.

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Observation of Soft-Rot Wood Degradation Caused by Higher Ascomyceteous fungi

  • Lee, Yang-Soo
    • Mycobiology
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    • v.28 no.1
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    • pp.47-50
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    • 2000
  • The capability of higher ascomyceteous fungi to cause typical soft-rot decay for wood under laboratory conditions is reviewed and discussed. Fungi tested were extremely active in the decomposition of timbers. Scanning electron micrographs illustrated typical soft-rot decay pattern of higher wood decay ascomycetes, with the exception of H. trugodes that caused white-rot decay. Most of the fungi tested could be grouped as soft-rot fungi that showed typical soft-rot type II. Hypha confined primarily to the resin canals in softwoods or vessel elements in hardwoods and spread tracheid to tracheid via pits of cell wall to cell wall with mechanical force.

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Effects of Gojineumja on Immunosuppression Induced by Methotrexate in Rat (고진음자(固眞飮子)가 methotrexate로 유발된 흰쥐의 면역기능저하(免疫機能低下)에 미치는 영향(影響))

  • Kang, Dae-Won;Kang, Seok-Bong
    • The Journal of Internal Korean Medicine
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    • v.25 no.4
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    • pp.117-128
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    • 2004
  • Objectives : To examine the effects of Gojineumja on white rats which deteriorated immunity caused by Methotrexate(MTX), first of all, MTX was fed to the rats once a day for 4 day. Methods : After the immune response of the rats are deteriorated, dried extracts of Gojineumja(GJE) mixed in water was fed to the white rats once a day for l4days. The next conclusion was made by examining the rates of B-cells and T-cells of the peripheral blood and the changes in rates of CD4+ T-cells and CD8+ T -cells of the blood sampled from the spleen and peripheral region. Especially the count of CD3+ CD4+ T-cells of the peripheral blood and the count of CD3+ CD4+ T-cells of the spleen the count of CD4+/ CD8+ T-cell of the peripheral blood and the spleen proved the significant effect of increasing immune responses statistically. Results :(1) The following are the summary of the results. (2) The percentage of B lymphocyte of peripheral blood was increased significantly in GJE group as compared with control group. (3) The percentage of CD3+ CD4+ T-cell of peripheral blood was increased significantly in GJE group as compared with control group. (4) The percentage of CD3+ CD8+ T-cell of peripheral blood was not different statistically. (5) The percentage of CD4+/ CD8+ T-cell of peripheral blood was increased significantly in GJE group as compared with control group. (6) The percentage of CD3+ CD4+ T-cell of spleen was increased significantly in GJE group as compared with control group. (7) The percentage of CD3+ CD8+ T-cell was not different statistically. (8) The percentage of CD4+ /CD8+ T-cell was not different statistically. Conclusions : Gojineumja has an effect of increasing immune responses on white rats with deteriorated immunity caused by MTX.

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Distribution of the neuropeptide Y immunoreactive neurons in the olfactory bulb of striped field mouse(Apodemus agrarius) (야생등줄쥐(Apodemus agrarius) 후각망울의 neuropeptide Y 면역반응세포의 분포)

  • Jeong, Young-gil;Kim, Kil-soo;Jung, Ju-young;Lee, Nam-seob;Lee, Kyeng-youl;Kim, Moo-kang
    • Korean Journal of Veterinary Research
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    • v.39 no.3
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    • pp.407-416
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    • 1999
  • This study was carried out to investigate the NPY-immunohistochemical characteristics of the olfactory bulb in the striped field mouse(Apodemus agrarius). The animals were anesthesized with thiopental sodium and perfused with 4% paraformaldehyde through left ventricle and aorta. Brains were removed and tranfered 10%, 20% and 30% sucrose. Sections were then cut on a cryostat into $40{\mu}m$-thick. The tissue immunostained with avidin-biotinylated complex method. The main olfactory bulb consisted of seven circumferential laminae : an olfactory nerve fiber layer, a glomerular layer with glomeruli surrounding by periglomerular cells, an external plexiform layer having granule and tufted cells, a mitral cell layer, a narrow internal plexiform layer, a granule cell layer forming several cell rows and a layer of white matter. The accessory olfactory bulb had four layers : an olfactory or vomeronasal nerve fiber layer, a glomerular layer consisting of small glomeruli, a mixed layer not distinguishing the external plexiform/mitral cell/granule cell layers and a granule cell layer. Most of NPY-immunoreactive(NPY-IR) neurons in main olfactory bulb were localized in the deeper portion of granule cell layer, white matter and anterior olfactory nucleus. In addition, some NPY-IR neurons were identified in the external plexiform layer. The shape of NPY-IR neurons of all olfactory bulb were predominant round or oval, sometime multipolar in shape. And most NPY-IR processes were parallel to long axis of white matter. In accessory olfactory bulb, NPY-IR neurons were not found in all region.

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The Effects of Aerobic Exercise on Body Temperature and Blood Components on Smoking Male University Students (남자 대학생들의 흡연여부에 따른 유산소운동이 체열과 혈액 검사치에 미치는 영향)

  • Lee, Jin;Bang, Hyun-Soo
    • Journal of Korean Physical Therapy Science
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    • v.24 no.2
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    • pp.1-8
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    • 2017
  • Background: To determine the effect of Aerobic exercise(AE) on body temperature and blood components in smoking male subjects. Methods: 15 subjects were randomly assigned to Smoking group(smoking, n=8) and Non-smoking group(Non-smoking, n=7). To measure body temperature and blood components. For evaluation of body temperature, the Infrared Thermography, IT was used, and blood components was measured using the Complete Blood cell Count(CBC). Results: The results shows that White blood cell (WBC) was significantly (p<0.05) increased in Smoking group. and no significantly difference between groups(p<0.05). Regarding body temperature, was significantly (p<0.05) increased in Smoking group and Non-somking group. and no significantly difference between groups(p<0.05). Conclusion: Aerobic exercise can increase White blood cell and body temperature in smokers.

Temperature Dependency of Non-dispersive Infrared Carbon Dioxide Gas Sensor by Using White-Cell Structure (White-Cell 구조를 응용한 비분산 적외선 이산화탄소 센서의 온도특성)

  • Yi, SeungHwan;Park, YoungHwan;Lee, JaeKyung
    • Journal of Sensor Science and Technology
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    • v.25 no.5
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    • pp.377-381
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    • 2016
  • NDIR $CO_2$ gas sensor was prototyped with ASIC implemented thermopile sensor which included temperature sensor and White-Cell structure in this paper. The temperature dependency of dual infrared sensors ($CO_2$ and reference IR sensors) has been characterized and their output voltage ratios according to the temperature and gas concentration were presented in this paper for achieving temperature compensation algorithm. The initial output voltages of NDIR $CO_2$ gas and reference IR sensors showed $3^{rd}$ order polynomial and linear output voltages according to the variation of ambient temperatures from 253 K to 333 K, respectively. The output voltages of temperature sensor presented a linear dependency according to the ambient temperature and could be described with V(T) = -3.0069+0.0145T(V). The characteristics of output voltage ratios could be modeled with five parameters which are dependent upon the ambient temperatures and gas concentration. The estimated $CO_2$ concentrations showed relatively high error below 300 ppm (maximum 572 % at 7 ppm $CO_2$ concentration), however, as the concentration increased from 500 ppm to 2,000 ppm, the overall estimated errors of $CO_2$ concentrations were less than ${\pm}10%$ in this research.