Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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Biocompatibility of bioaggregate cement on human pulp and periodontal ligament (PDL) derived cells

사람의 치수 및 치주인대 세포에 대한 Bioaggregate 시멘트의 생체적합성에 대한 연구

  • Chung, Choo-Ryung (Department of Orthodontics, Yonsei University College of Dentistry) ;
  • Kim, Eui-Seong (Department of Conservative Dentistry, Yonsei University College of Dentistry) ;
  • Shin, Su-Jung (Department of Conservative Dentistry, Yonsei University College of Dentistry)
  • 정주령 (연세대학교 치과대학 치과교정과) ;
  • 김의성 (연세대학교 치과대학 치과보존과) ;
  • 신수정 (연세대학교 치과대학 치과보존과)
  • Received : 2010.09.15
  • Accepted : 2010.10.17
  • Published : 2010.11.30
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Abstract

Objectives: This study was performed to investigate the biocompatibility of newly introduced Bioaggregate on human pulp and PDL cells. Materials and Methods: Cells were collected from human pulp and PDL tissue of extracted premolars. Cell culture plate was coated either with Bioaggregate or white MTA, then the same number of cells were poured to cell culture dishes. Cell attachment and growth was examined under a phase microscope after 1,3 and 7 days of seeding. Cell viability was measured and the data was analyzed using Student t-test and one way ANOVA. Results: Both types of cells used in this study were well attached and grew healthy on Bioaggregate and MTA coated culture dishes. No cell inhibition zone was observed in Bioaggregate group. There was no statistical difference of viable cells between bioaggreagte and MTA groups. Conclusions: Bioaggregate appeared to be biocompatible compared with white MTA on human pulp and PDL cells.

연구목적: 본 연구는 인간의 치수 및 치근단 조직에서 유리된 세포를 이용하여 비교적 최근 소개된 Bioaggregate의 생체친화성을 평가하는 데에 있다. 연구 재료 및 방법: 사람의 발거치로 부터 치수 및 치근단 조직에서 배양된 세포를 이용하였다. Bioaggregate와 white MTA를 혼합하여 세포배양판에 적용한 후 같은 수의 세포를 배양하였다. 1, 3, 그리고 7일 후 위상차현미경을 사용하여 세포의 부착과 성장을 관찰하고 cell viability test를 시행하였다. 얻어진 결과는 Student t-test및 one way ANOVA를 이용하여 분석하였다. 결과: 두가지 종류의 세포 모두 Bioaggregate와 MTA가 혼합된 배양판에서 잘 성장하였으며 Bioaggregate군에서는 inhibition zone이 관찰되지 않았다. Cell viability test에서 두 그룹간 통계적인 유의성 차이는 없었다. 결론: Bioaggreagete는 치수 및 치근단 세포에 대하여 MTA와 유사한 세포친화성을 보였다.

Keywords

References

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