Browse > Article

Anti-Helicobacter pylori, Cytotoxic, and Anti-inflammatory Activities of White Ginseng Extract  

Jee, Hee-Sook (Division of Animal Life Science, Konkuk University)
Chang, Kyung-Hoon (Division of Animal Life Science, Konkuk University)
Moon, Sun-Hee (Division of Animal Life Science, Konkuk University)
Park, Se-Ho (Dongwon Korean Ginseng Co., Ltd.)
Paik, Hyun-Dong (Division of Animal Life Science, Konkuk University)
Publication Information
Food Science and Biotechnology / v.17, no.5, 2008 , pp. 1106-1109 More about this Journal
Abstract
The anti-Helicobacter pylori activity, cytotoxicity, and anti-inflammatory activity of white ginseng extract (WGE) were investigated in vitro in this study. The antimicrobial effects of WGE toward H. pylori strains 52 J99, SSI, and 51 were tested using the disk diffusion method. Among these H. pylori strains, H. pylori 52 was the most sensitive, having the largest inhibition zone (19 mm), followed by J99, SSI, and 51. The zone of inhibition due to WGE increased significantly with increasing dosage. The cytotoxicity of WGE toward the human cancer cell lines A-549 (human lung carcinoma), HEC-1-B (human endometrial adenocarcinoma), HeLa (human uterin adenocarcinoma), and SW-156 (human kidney carcinoma) was measured using the 3-(4,5-dimethylthizol-2-yl)-2,5-diphenylate-tetrazolium bromide (MTT) assay. WGE exhibited an inhibitory effect on cell growth at 2.0 mg/mL for all tumor cell lines. An analysis of anti-inflammatory activity using the RAW 264.7 cell line showed that the inhibition of nitric oxide (NO) production increased as the WGE content increased. These results demonstrate the potential of WGE to be used as a health-promoting substance.
Keywords
white ginseng extract; Helicobacter pylori; anti-Helicobacter pylori activity; cytotoxicity; anti-inflammatory activity;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Cheng Y, Shen LH, Zhang JT. Anti-amnestic and anti-aging effects of ginsenoside $Rg_1\;and\;Rb_2$ and its mechanism of action. Acta Pharmacol. Sin. 2: 143-149 (2005)
2 Wang BX, Cui JC, Liu AJ, Wu SK. Studies on the anti-fatigue effect of saponins of stem and leaves of Panax ginseng (SSLG). J. Tradit. Chin. Med. 2: 89-94 (1983)
3 Lu Zo, Dice JF. Ginseng extract inhibits protein degradation and stimulates protein synthesis in human fibroblasts. Biochem. Bioph. Res. Co. 1: 636-640 (1985)
4 Zhu S, Zou K, Cai S, Meselhy MR, Komatsu K. Simultaneous determination of triterpene saponins in Ginseng drugs by high performance liquid chromatography. Chem. Pharm. Bull. 52: 995-998 (2004)   DOI   ScienceOn
5 Lam SK, Ng TB. Pananotin, a potent antifungal protein from roots of the traditional Chinese medicinal herb Panax ginseng. Planta Med. 68: 1024-1028 (2002)   DOI   ScienceOn
6 Park SJ. Inhibition of red ginseng on 5-hydroxyeicosatetraenoic acid (5-HETE) biosynthesis from arachidonic acid in Helicobacter pylori-infected gastric cells. Nutr. Sci. 9: 152-158 (2006)   과학기술학회마을
7 Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65: 55-63 (1983)   DOI
8 Yun TK, Lee YS, Lee YH, Yun HY. Cancer chemopreventive compounds of red ginseng produced from Panax ginseng C.A. Meyer. J. Ginseng Res. 25: 107-111 (2001)
9 Ohshima H. Genetic and epigenetic damage induced by reactive nitrogen species: Implications in carcinogenesis. Toxicol. Lett. 140/141: 99-104 (2003)   DOI
10 Wu CF, Bi XL, Yang JY, Zhan JY, Dong YX, Wang JH, Wang JM, Zhang R, Li X. Differential effects of ginsenosides on NO and $TNF-{\alpha}$ production by LPS-activated N9 microglia. Int. Immunopharmacol. 7: 313-320 (2007)   DOI   ScienceOn
11 Lee JH, Shim JS, Lee JS, Kim MK, Chung MI, Kim KH. Pectinlike acidic polysaccharide from Panax ginseng with selective antiadhesive activity against pathogenic bacteria. Carbohyd. Res. 341: 1154-1163 (2006)   DOI   ScienceOn
12 Court WA, Hendal JG, Elmi J. Reversed-phase high performance liquid chromatographic determination of ginsenosides of Panax quinquefolium. J. Chromatogr. A 755: 11-17 (1996)   DOI   ScienceOn
13 Masuda H, Naohide K, Woo GJ, Shin IS. Inhibitory effects of gochoonangi (Wasabia japonica) against Helicobacter pylori and its urease activity. Food Sci. Biotechnol. 13: 191-196 (2004)
14 Jung NP, Song SO, Choi SU. Cytotoxicity of white and red ginseng against cancer cells and their effects on the cell cycle. J. Ginseng Res. 24: 183-187 (2000)
15 Surh YJ, Na HK, Lee JY, Keum YS. Molecular mechanisms underlying anti-tumor promoting activities of heat-processed Panax ginseng C.A. Meyer. J. Korean Med. Sci. 16: S38-S41 (2001)   DOI
16 Park S, Choue RW, Cho Y, Ziboh VA. Regional biosynthesis of prostaglandins and hypoxyeicosatetraenoic acids from arachidonic acid in the rat stomach tissue. Prostag. Leukotr. Ess. 68: 35-42 (2003)   DOI   ScienceOn
17 Park WB, Choung By, Park SM, Kim HS, Lyu SY. Effects of drying process of mistletoes on cytotoxicities against cultured HL-60 and Molt-4 cells. Food Sci. Biotechnol. 8: 391-396 (1999)
18 Bao HY, Zhang J, Yeo SJ, Myung CS, Kim HM, Kim JM, Park JH, Cho J, Kang JS. Memory enhancing and neiroprotective effects of selected ginsenosides. Arch. Pharm. Res. 3: 333-342 (2005)
19 Keum YS, Park KK, Lee JM, Chun KS, Park JH, Lee SK, Kwon H, Surh YJ. Antioxidant and anti-tumor promoting activities of the methanol extract of heat-processed ginseng. Cancer Lett. 150: 41-48 (2000)   DOI   ScienceOn
20 Rai D, Bhatia G, Sen T, Palit G. Anti-stress effects of Ginkgo biloba and Panax ginseng: A comparative study. J. Pharmacol. Sci. 4: 458-464 (2003)
21 Bae EA, Han MJ, Coo MK, Park SY, Kim DH. Metabolism of 20(S)- and 20(R)-ginsenoside $Rg_3$ by human intestinal bacteria and its relation to in vitro biological activation. Biol. Pharm. Bull. 25: 58-63 (2002)   DOI   ScienceOn
22 Kwon JH, Belanger JMR, Pare JRJ, Yaylayan V. Application of the microwave-assisted process (MAP) to the fast extraction of ginseng saponins. Food Res. Int. 36: 491-498 (2003)   DOI   ScienceOn
23 Kim SO, Park CW, Moon S, Lee HA, Kim B, Lee DU, Lee JH, Park J. Effects of high-hydrostatic pressure on ginsenoside concentration in Korean red ginseng. Food Sci. Biotechnol. 16: 848-853 (2007)   과학기술학회마을
24 Yuan CS, Wo JA. Ginsenoside variability in American ginseng samples. Am. J. Clin. Nutr. 75: 600-601 (2002)   DOI
25 Vuksan V, Sung MK, Sievenpiper JL, Stavro PM, Jenkins AL, Di Buono M, Lee KS, Leiter LA, Nam KY, Arnason JT, Choi M, Naeem A. Korean red ginseng (Panax ginseng) improves glucose and insulin regulation in well-controlled, type 2 diabetes: Results of a randomized, double-blind, placebo-controlled study of efficacy and safety. Nutr. Metab. Cardiovas. 21: 1-11 (2006)