• The Journal of the Korean Society for Microbiology
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• v.18 no.1
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• pp.67-71
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• 1983

Antisera to ox red blood cells were prepared by injection of ox red blood cell stroma without adjuvant in outbred white rabbits. Purified IgM fraction for T-cell subset assay was obtained from these rabbit anti-ox red blood cell stroma antisera by precipitation with 50% saturated ammonium sulphate followed by DEAE-Cellulose chromatography and Sephadex G-200 gel filtration. The serological identification of purified IgM fraction was achieved by immunoelectrophoresis with guinea pig antiserum against rabbit anti-ox red blood cell IgM antibody.

• Journal of the korean veterinary medical association
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• v.26 no.1
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• pp.38-42
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• 1990

The effect of Aloe vera administration(500mg/kg, P.O.) for 21 days on the total white blood cell, packed cell volume, hemoglobin, total protein, serum glutamic pyruvic transaminase and sorbitol dehydrogenase values in cobalt-60 gamma irradiated rats was s

• Genomics & Informatics
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• v.8 no.3
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• pp.122-130
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• 2010

Abnormal hematological values are associated with various disorders including cancer and cardiovascular, metabolic, infectious, and immune diseases. We report the copy number variations (CNVs) in clinically relevant hematological parameters, including hemoglobin level, red and white blood cell counts, platelet counts, and red blood cell (RBC) volume. We describe CNVs in several loci associated with these hematological parameters in 8,842 samples from Korean population-based studies. The data that we evaluated included four RBC parameters, one platelet parameter, and one associated with total white blood cell (WBC) count, exceeding the genome-wide significance. We show that CNVs in hematological parameters are associated with some loci, different from previously associated loci reported in single nucleotide polymorphism (SNP) association studies.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.20 no.2
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• pp.58-63
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• 2012

An altitude chamber, also known as a hypobaric chamber, is a device used during aerospace or high terrestrial altitude research or training to simulate the effects of high altitude on the human body. Although data from altitude chamber researches using experimental animals have been accumulated, studies in the humans exposed to hypobaric conditions are seldomly reported. Despite the importance of altitude chamber flight training in the field of aviation physiology, the hematological analysis of post-flight physiological changes has rarely been performed. The aims of the present study were to investigate the alterations in blood components during altitude chamber flight and to determine whether the differences between pre- and post-flight values are significant. Sixty experienced pilots in the Republic of Korea Air Force were enrolled in the altitude chamber flight training. Venous blood samples were obtained before and immediately after the flight. Compared with the pre-flight values($6.32{\times}10^3/mm^3$, $5.02{\times}10^6/mm^3$, 15.61 g/dL, respectively), white blood cell count, red blood cell count and hemoglobin level were significantly increased after the flight($6.77{\times}10^3/mm^3$, $5.44{\times}10^6/mm^3$, 16.26 g/dL; p=0.006, p=0.012, p<0.001, respectively). These alterations may be attributable to the exposure to hypobaric hypoxia, 100% oxygen supply for denitrogenation, considerable rise and fall in altitude and psychophysical stress due to these factors. In further studies, experimental groups and methods should be individualized to ensure objectivity and diversification. In addition, multiple time-frame analyses regarding the changing pattern of each blood component are also required to elucidate the physiological process for adapting to the high terrestrial altitude exposure.

• Environmental Analysis Health and Toxicology
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• v.3 no.1_2
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• pp.55-64
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• 1988

Experiments were performed on mice to investigate the influences of ampicillin and ethanol on the immune response. Ampicillin was injected intraperitoneally and ethanol was administered in the drinking water. Mice were sensitized and challenged with sheep red blood cells. Immune responses were evaluated by humoral immunity, cellular immunity, peripheral circulating white blood cell and phagocyte activity. 1. The combined administration of ampicillin and ethanol as compared to ampicillin had not influence on the weight of spleen, but increased the weight of thymus. 2. Humoral immune response was slightly reduced by ampicillin. Especially, the combined administration of ampicillin and ethanol significantly reduced hemclysin production. 3. Cellural immune response was reduced by ampicillin. The combine administration of ampicillin and ethanol significantly reduced cellural immune response. 4. Peripheral circulating white blood cell was reduced by the combined administration of ampicillin and ethanol as compared to ampicillin. 5. The combined administration of ampicillin and ethanol as compared to ampicillin had not influence on the phagocyte activity.

• Environmental Analysis Health and Toxicology
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• v.3 no.1_2
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• pp.9-19
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• 1988

The effect of rancid perilla oil on the immune response in mice was studied. ICR male mice were divided into 5 groups and were fed on the experimental diets for 4 weeks. Mice were sensitized and challenged with sheep red blood cell. Immune responses were evaluated by antibody production, Arthus reaction, delayed type hypersensitivity (DTH), Rosette forming cell and macrophage activity. Biochemical items were measured by serum protein and serum albumin. The weight of spleen, thymus and liver were measured. The rancid perilla oil diets decreased humoral and cellular immune responses, the number of peripheral circulating white blood cells and total protein and serum albumin. These results showed that the high rancid perilla oil diet decreased more humoral and cellular immune response, the number of peripheral circulating white blood cells, and total protein and serum albumin than the low rancid perilla oil diet did.

• Environmental Analysis Health and Toxicology
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• v.4 no.1_2
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• pp.1-10
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• 1989

Experiments were performed on mice to investigate the influences of doxycycine and ethanol on the immune responses. Doxycycline was injected intraperitoneally and ethanol was administered in the drinking water. Mice were sensitized and challenged with sheep red blood cells. Immune responses were evaluated by humoral immunity, cellular immunity, peripheral circulating white blood cell and phagocyte activity. Pathotoxicological influences were measured by serum protein and albumin. The weight of spleen, thymus and liver were measured. Doxycline and ethanol combined administration decreased the weight of thymus and spleen. Humoral and cellular immune response were reduced by doxycycline administration. Especially ethanol combined administration significantly reduced humoral and cellular immune response. Phagocyte activity was increased by ethanol combined administration and peripheral circulating white blood cell was significantly increased by ethanol adminstration. Ethanol combined administration decreased serum A/G ratio.

• Korean Journal of Pharmacognosy
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• v.11 no.2
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• pp.104-107
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• 1980

Mansam, the root of Codonopsis pilosula (Campanulaceae) has a action on blood metabolism. It has been known to possess anti leukemic effect by increasing of red blood cell, at same time, by decreasing white blood cell, with this connection, present study is aimed to clarify such potential anti leukenic effect by testing ethanol extract and chloroform extract against murine tumor model, lymphocytic leukemia P388. The data indicated that these extracts appeared to be inactive against this tumor line.

• Journal of the Korean Burn Society
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• v.22 no.2
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• pp.38-44
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• 2019

Purpose: The immature granulocyte count has been reported to be a marker of infection and sepsis. The difference in leukocyte subfractions (delta neutrophil index, DNI) in ADVIA 2120 reflects the fraction of circulating immature granulocytes in the blood. This study evaluated the clinical utility of DNI as a severity and prediction marker in critically ill patients with burn sepsis. Methods: One hundred and sixty nine patients admitted to the burn care unit were studied. DNI (the difference in leukocyte subfractions identified by myeloperoxidase and nuclear lobularity channels) was determined using a specific blood cell analyzer. Results: Seventy one patients (42 %) were diagnosed with burn sepsis. DNI was significantly higher in patients with burn sepsis than in patients without (P<0.01). Delta neutrophil index was a better indicator of burn sepsis than C-reactive protein, lactate, white blood cell count, HCO3, base excess, lactate, procalcitonin (odds ratio, 6.31; confidence interval 2.36~16.90; P<0.01). And the receiver operating characteristic curves showed that delta neutrophil index, AUC 0.795 (95% confidence interval, 0.721~0.869; P<0.05) was a better predictor of burn sepsis than lactate, procalcitonin, white blood cell, base excess and abbreviated burn severity index. Conclusion: Delta neutrophil index may be used as a early marker of patients with burn sepsis.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.14
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• pp.5733-5739
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• 2014

Background: Breast cancer is the most common female malignancy in the world. Beta glucan can be a hematopoietic and an immune modulator agent in cancer patients. The aim of this trial was to determine the effect of beta glucan on white blood cell counts and serum levels of IL-4 and IL-12 in women with breast cancer undergoing chemotherapy. Materials and Methods: This randomized double-blind placebo-controlled clinical trial was conducted on 30 women with breast carcinoma aged 28-65 years. The eligible participants were randomly assigned to intervention (n=15) or placebo (n=15) groups using a block randomization procedure with matching based on age, course of chemotherapy and menopause status. Patients in the intervention group received two 10-mg capsules of soluble 1-3, 1-6, D-beta glucan daily and the control group receiving placebo during 21 days, the interval between two courses of chemotherapy. White blood cells, neuthrophil, lymphocyte and monocyte counts as well as serum levels of IL-4 and IL-12 were measured at baseline and at the end of the study as primary outcomes of the study. Results: In both groups white blood cell counts decreased after 21 days of the intervention, however in the beta glucan group, WBC was less decreased non significantly than the placebo group. At the end of the study, the change in the serum level of IL-4 in the beta glucan group in comparison with the placebo group was statistically significant (p=0.001). The serum level of IL-12 in the beta glucan group statistically increased (p=0.03) and comparison between two groups at the end of the study was significant after adjusting for baseline values and covariates (p=0.007). Conclusions: The findings suggest that beta glucan can be useful as a complementary or adjuvant therapy and immunomodulary agent in breast cancer patients in combination with cancer therapies, but further studies are needed for confirmation.