The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Purification of Anti-Ox Red Blood Cell IgM Antibody for T-cell Subset Assay

T-cell subset 정량(定量)을 위한 항우적혈구(抗牛赤血球) IgM 항체(抗體)의 분리(分離) 정제(精製)(II)

  • Ha, Youn-Mun (Department of Microbiology, School of Medicine, Kyung Hee University) ;
  • Ho, Soon-Tae (Department of Microbiology, School of Medicine, Kyung Hee University)
  • 하윤문 (경희대학교 의과대학 미생물학교실) ;
  • 호순태 (경희대학교 의과대학 미생물학교실)
  • Published : 1983.12.31
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Abstract

Antisera to ox red blood cells were prepared by injection of ox red blood cell stroma without adjuvant in outbred white rabbits. Purified IgM fraction for T-cell subset assay was obtained from these rabbit anti-ox red blood cell stroma antisera by precipitation with 50% saturated ammonium sulphate followed by DEAE-Cellulose chromatography and Sephadex G-200 gel filtration. The serological identification of purified IgM fraction was achieved by immunoelectrophoresis with guinea pig antiserum against rabbit anti-ox red blood cell IgM antibody.

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