• BMB Reports
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• 제39권1호
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• pp.61-67
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• 2006

Molecular co-suppression phenomena are important to consider in transgene experiments. Embryogenic cells were obtained from immature cotyledons and engineered with two different gene constructs (pHV and pHVS) through particle bombardment. Both constructs contain a gene conferring resistance to hygromycin (hpt) as a selective marker and a modified glycinin (11S globulin) gene (V3-1) as a target. sGFP(S65T) as a reporter gene was, however, inserted into the flanking region of the V3-1 gene (pHVS). Fluorescence microscopic screening after the selection of hygromycin, identified clearly the expression of sGFP(S65T) in the transformed soybean embryos bombarded with the pHVS construct. Stable integration of the transgenes was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. Seeds of transgenic plants obtained from the pHV construct frequently lacked an accumulation of endogenous glycinin, which is encoded by homologous genes to the target gene V3-1. Most of the transgenic plants expressing sGFP(S65T) showed highly accumulation of glycinin. The expression of sGFP(S65T) and V3-1 inherits into the next generations. sGFP(S65T) as a reporter gene may be useful to increase the transformation efficiency of transgenic soybean with avoiding gene co-suppression.

• Applied Biological Chemistry
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• 제40권4호
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• pp.271-276
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• 1997

박테리오파아지 T7 RNA polymerase 유전자를 식물체내에서 이용할 수 있을지 알아보기 위하여 상처유발인 감자 단백질 분해효소 억제제 유전자의 프로모터에 박테리오파지 T7 RNA polymerase 유전자를 연결시킨 후 담배에 도입시켰다. 형질전환 식물체의 DNA에 대한 Southern hybridization에 의하면 T7 RNA polymerase 유전자가 식물체내에 1-2 copy가 존재하며, Northern hybridization에 의하면 T7 RNA polymerase의 RNA가 상처에 따라 생성되는 것을 확인하였다. 또한 Western hybridization에 의하면 식물체내 T7 RNA polymerase 단백질이 생성되는데 그 크기는 대장균에서 생성되는 단백질 크기와 유사한 80 kDa 이었으며 시험관내에서 전사체에 뉴클레오타이드를 결합시키는 능력이 있음도 확인하였다. 따라서 T7 RNA polymerase 유전자를 이용하여 식물체내에서 원하는 유전자의 발현을 증대시킬 수 있을 것으로 사료된다.

• BMB Reports
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• 제46권10호
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• pp.495-500
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• 2013

Turnip yellow mosaic virus (TYMV) is a positive strand RNA virus. We have modified TYMV coat protein (CP) by inserting a c-Myc epitope peptide at the N- or C-terminus of the CP, and have examined its effect on assembly. We introduced the recombinant CP constructs into Nicotiana benthamiana leaves by agroinfiltration. Examination of the leaf extracts by agarose gel electrophoresis and Western blot analysis showed that the CP modified at the N-terminus produced a band co-migrating with wild-type virions. With C-terminal modification, however, the detected bands moved faster than the wild-type virions. To further examine the effect, TYMV constructs producing the modified CPs were prepared. With N-terminal modification, viral RNAs were protected from RNase A. In contrast, the viral RNAs were not protected with C-terminal modification. Overall, the results suggest that virion assembly and RNA packaging occur properly when the N-terminus of CP is modified, but not when the C-terminus is modified.

• 영재교육연구
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• 제23권6호
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• pp.919-945
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• 2013

영재성의 의미와 특성 탐색은 후속되는 영재 판별 및 교육과정 운영을 위한 전제조건이다. 즉, 체계와 내실을 갖춘 영재교육의 운영을 위해서는 특정 영재성의 의미와 세부 특성에 관한 분석이 우선적 과제이다. 이에 이 연구는 예술영재성의 범주에 속하는 음악 및 전통예술, 발레 영재성 관련 국내외 문헌 및 사례를 중심으로 각 영재성의 의미와 특성을 분석하고 비판적 시각에서 논의를 전개하였다. 그 결과, 음악 및 전통예술(전통음악, 전통무용, 연희), 발레 분야 영재성의 심리학적 구인과 세부 특성을 확인할 수 있었고, 각 영역 특성 간 공통점과 차이점을 비교분석함으로써 예술영재가 지닌 내재적 특성 요인과 외재적 조건 요인을 수렴할 수 있었다. 이 연구의 결과가 예술영재성에 관한 논의 및 후속 연구의 토대가 되기를 기대한다.

• 수완나부미
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• 제12권2호
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• pp.157-200
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• 2020

This paper explores critically and historically some of the popular academic views concerning the development of the study of Southeast Asia through the lens of the contributions of particular scholars and institutions. Within the broad field of Southeast Asian Studies the focus is on the disciplines of geography, history and ethnology. There are certain views concerning the development of scholarship on Southeast Asia which continue to surface and have acquired, or are in the process of acquiring "mythical" status. Among the most enduring is the claim that the region is a post-Second World War construction primarily arising from Western politico-strategic and economic preoccupations. More specifically, it is said that Southeast Asian Studies for a considerable period of time has been subject to the American domination of this field of scholarship, located in programs of study in such institutions as Cornell, Yale and California, Berkeley, and, within those institutions, focused on particular scholars who have exerted considerable influence on the directions which research has taken. Another is that, based on the model or template of Southeast Asian Studies (and other area studies projects) developed primarily in the USA, it has distinctive characteristics as a scholarly enterprise in that it is multidisciplinary, requires command of the vernacular, and assigns special importance to what has been termed 'groundedness' and historical, geographical and cultural contextualization; in other words, a Southeast Asian Studies approach as distinct from disciplinarybased studies addresses local concerns, interests, perspectives and priorities through in-depth, on-the-ground, engaged scholarship. Finally, views have emerged that argue that a truly Southeast Asian Studies project can only be achieved if it is based on a set of locally-generated concepts, methods and approaches to replace Western ethnocentrism and intellectual hegemony.

• 한국실내디자인학회논문집
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• 제17호
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• pp.216-222
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• 1998

With the continued integration through scientification and the progress I the technology of construction around the world Korea's inability to stay within the mainstream of the movement has put it in danger of losing it's very identity. As such Korean cityscapes are filled with overwhelming prevalence of buildings and constructs void of national identity but rather reflective of unconditional application of the western standards of architecture which has so far been the compass of the movement. Accordingly the purpose of this research is the understanding of the current world view concerning of architecture the critical regionalism through the examination of the background of theory which reflect the architectural Kenneth Prampton and also through Ricardo Legorrata Charles Correa Antoine Predock's analysis. Furthermore this research hopes to explore and offer a methodology through which a unique Korean identity can be established in Korea's Modern architecture.

• Asia Marketing Journal
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• 제18권1호
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• pp.75-97
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• 2016

Globalization and the emergence of new markets has placed increased emphasis on cross-cultural sales and marketing research. Despite considerable advances in the personal selling literature, little is known about the cross-cultural transferability of key constructs. Given the degree to which well-accepted relational sales behaviors such as ASB and customer oriented selling reflect Western values such as individualism and low uncertainty avoidance, the relative efficacy of such practices in alternative cultural context is less clear. Using a Korean sample, our results confirm the beneficial direct effects of these relational selling practices upon performance. However, we also find strong workgroup interdependence diminishes the effects of ASB and that performance fully mediates the relationship between both sales behaviors and job satisfaction. In contrast to prior results, we find female salespeople and those newer to their position demonstrate stronger ASB.

• 생명과학회지
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• 제17권3호통권83호
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• pp.356-361
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• 2007

${\beta}-catenin$과 결합하는 ErbB2의 부위를 조사하기 위하여 proteasome에 의하여 분해되지 않는 ${\beta}-catenin$과 다양한 ErbB2 construct를 COS7 세포에 transfection한 후 ErbB2 단백질을 그것의 항체로 가라앉혔다. 이 때 공침한 ${\beta}-catenin$을 Western blot으로 분석하였다. C 말단에서부터 잘려진 ErbB2 단백질 중에 kinase 영역을 가지고 있는 것들만 ${\beta}-catenin$과 공침하였다. kinase 영역의 필요성을 확인하기 위하여 kinase 영역이 내부에서 제거된 ErbB2 construct를 ${\beta}-catenin$과 transfection 한 후 동일한 실험을 실시하였다. 이 실험에서 ${\beta}-catenin$는 kinase 영역이 내부적으로 제거된 ErbB2 단백질과 공침하지 않았다. 이 결과는 ${\beta}-catenin$과 결합하는 ErbB2의 위치는 kinase 영역내에 있음을 제시한다.

• 대기
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• 제28권3호
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• pp.291-303
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• 2018

In this study, a new consensus technique for predicting tropical cyclone (TC) intensity in the western North Pacific was developed. The most important feature of the present consensus model is to select and combine the guidance numerical models with the best performance in the previous years based on various evaluation criteria and averaging methods. Specifically, the performance of the guidance models was evaluated using both the mean absolute error and the correlation coefficient for each forecast lead time, and the number of the numerical models used for the consensus model was not fixed. In averaging multiple models, both simple and weighted methods are used. These approaches are important because that the performance of the available guidance models differs according to forecast lead time and is changing every year. In particular, this study develops both a multi-consensus model (M-CON), which constructs the best consensus models with the lowest error for each forecast lead time, and a single best consensus model (S-CON) having the lowest 72-hour cumulative mean error, through on training process. The evaluation results of the selected consensus models for the training and forecast periods reveal that the M-CON and S-CON outperform the individual best-performance guidance models. In particular, the M-CON showed the best overall performance, having advantages in the early stages of prediction. This study finally suggests that forecaster needs to use the latest evaluation results of the guidance models every year rather than rely on the well-known accuracy of models for a long time to reduce prediction error.

• Molecules and Cells
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• 제26권6호
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• pp.554-557
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• 2008

Double-stranded RNA (dsRNA) induces gene silencing in a sequence-specific manner by a process known as RNA interference (RNAi). The RNA-induced silencing complex (RISC) is a multi-subunit ribonucleoprotein complex that plays a key role in RNAi. VIG (Vasa intronic gene) has been identified as a component of Drosophila RISC; however, the role VIG plays in regulating RNAi is poorly understood. Here, we examined the spatial and temporal expression patterns of VIG-1, the C. elegans ortholog of Drosophila VIG, using a vig-1::gfp fusion construct. This construct contains the 908-bp region immediately upstream of vig-1 gene translation initiation site. Analysis by confocal microscopy demonstrated GFP-VIG-1 expression in a number of tissues including the pharynx, body wall muscle, hypodermis, intestine, reproductive system, and nervous system at the larval and adult stages. Furthermore, western blot analysis showed that VIG-1 is present in each developmental stage examined. To investigate regulatory sequences for vig-1 gene expression, we generated constructs containing deletions in the upstream region. It was determined that the GFP expression pattern of a deletion construct (${\Delta}-908$ to -597) was generally similar to that of the non-deletion construct. In contrast, removal of a larger segment (${\Delta}-908$ to -191) resulted in the loss of GFP expression in most cell types. Collectively, these results indicate that the 406-bp upstream region (-596 to -191) contains essential regulatory sequences required for VIG-1 expression.