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http://dx.doi.org/10.5352/JLS.2007.17.3.356

ErbB2 kinase domain is required for ErbB2 association with β-catenin  

Ha, Nam-Chul (Research Institute for Drug Development, College of Pharmacy, Pusan National University)
Xu, Wanping (Urologic Oncology Branch, National Cancer Institute)
Neckers, Len (Urologic Oncology Branch, National Cancer Institute)
Jung, Yun-Jin (Research Institute for Drug Development, College of Pharmacy, Pusan National University)
Publication Information
Journal of Life Science / v.17, no.3, 2007 , pp. 356-361 More about this Journal
Abstract
To investigate the region of ErbB2 for the $ErbB2-{\beta}-catenin$ interaction, a proteasome $resistant-{\beta}-catenin$ and various ErbB2 constructs were transfected in COS7 cells. ErbB2 proteins were immunoprecipitated, and coimmunoprecipitated ${\beta}-catenin$ was examined by Western blotting. ${\beta}-catenin$ coimmunoprecipitated with full length ErbB2. Of the truncated ErbB2 proteins DT (1-1123), DHC (1-1031) and DK (1-750), the ErbB2 constructs containing the kinase domain, DT and DHC, precipitated together with ${\beta}-catenin$ but DK containing no kinase domain did not. To further test the requirement of the kinase domain for ${\beta}-catenin-ErbB2$ interaction, the presence of ${\beta}-catenin$ in the immunocomplex was examined following transfection with an ErbB2 mutant (${\triangle}750-971$) whose kinase domain is internally deleted and subsequent immunoprecipitation of the ErbB2 mutant. ${\beta}-catenin$ was not detected in the immunocomplex. These results suggest that the ErbB2 kinase domain comprises a potential site for ${\beta}-catenin$ binding to the receptor tyrosine kinase.
Keywords
${\beta}-catenin$; ErbB2; protein association;
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