• Title/Summary/Keyword: Welding Length

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DNA damages with Fpg/Endo Ⅲ FLARE Assay in cynomolgus monkeys exposed to stainless steel welding fume (용접흄 흡입노출 영장류에서 Fpg/Endo Ⅲ FLARE Assay를 이용한 DNA 손상 및 회복)

  • Rim, Kyung Taek;Kim, Soo Jin;Chung, Yong Hyun;Kim, Hyeon Yeong;Maeng, Seung Hee;Yu, Il Je
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.17 no.4
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    • pp.272-281
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    • 2007
  • 선박제조업을 비롯한 운송업 및 건축업 등의 다양한 분야에서 용접기술이 이용되어 옴에 따라 용접근로자들에 대한 산업보건학적 관심이 높아지고 있다. 노출정도가 다양하기는 하지만 용접흄은 6가 크롬을 비롯한 금속화합물과 유해가스, 화학물질 등을 복합적으로 포함하고 있는 스테인레스 스틸 용접흄에 대한 유전독성영향을 평가하기 위하여 흡입챔버를 이용, 실험동물인 영장류에 스테인레스 스틸 용접흄을 노출시키고 혈액 내 lymphocytes에 생성된 용접흄 노출농도 및 시간별 DNA 손상정도 및 그 회복효소를 측정함으로써, 유해성이 완전하게 확인되지 않은 용접흄에 노출되어 나타날 수 있는 암을 비롯한 심각한 건강영향을 예방하기 위한 각 지표들을 찾아 그 유용성을 비교하고자 하였다. 영장류를 노출시키기 위해 robotic arm을 장치한 영장류 흡입노출 시스템을 개발하였으며, 이 노출 시스템을 이용하여 수컷 영장류 6마리에 대해 용접흄 노출시험을 실시하였는데 실험군은 대조군 2, 저농도 ($31mg/m^3$) 노출군 2, 고농도 ($63mg/m^3$) 노출군 2마리로 구성하였고, 1일 2시간씩 일주일에 5일 동안 용접흄에 노출시켰다. 노출 농도는 지속적으로 모니터링 하였고, 노출과정 중에 영장류의 혈액을 채취하여 lymphocytes를 분리, 단세포 DNA 손상을 선별하기 위해 DNA 손상회복 효소인 E. coli formamidopyrimidine-DNA glycosylase (Fpg)와 endonuclease Ⅲ (Thymine Glycol-DNA glycosylase) 투여와 Comet asaay (single cell gel electrophoresis, 단세포겔전기영동기법)를 결합시켜 이용하는 Fpg/Endo III FLARE 분석법을 사용하였다. Fpg enzyme에 의한 olive tail moment값의 변화는 16주 노출군부터 노출부검(34주)군 까지 노출농도가 높아짐에 따른 olive tail moment 기하평균 값의 양 반응관계를 보기는 어렵지만, 고농도군의 경우 27주 노출군에서 가장 높은 olive tail moment 값을 보이고 이후 차츰 감소하였다. 한편 16주에서 22주까지의 노출기간에서는 대조군에 비해 노출군에서 DNA손상정도(olive tail moment값)는 모두 유의하게 높았으나, 6, 12, 18, 25, 31, 33, 35주간 노출하였을 때는 다른 결과를 보였다. 각 실험군의 Fpg enzyme에 의한 tail length값의 분포를 살펴볼 때, 저농도군 및 고농도군에서 27주간 노출하였을 때 가장 높은 tail length 값을 보이고 이후 차츰 감소하는 경향을 보였다. 또한 16, 22주간 노출하였을 때 대조군에 비해 노출군에서 tail length 값이 유의하게 높았으나, 20주간에서만 양 반응관계가 관찰되었고, 다른 주간에서는 양 반응 및 기간 반응관계를 나타내지는 않았다. Endo III enzyme에 의한 olive tail moment값의 변화는 기간별 노출군에서 대조군에 비해 높은 DNA손상정도(olive tail moment값)를 나타내는 결과들이 있었지만, 10, 12, 16, 22, 25, 31주간 노출하였을 때 등 상당수 노출기간에서 반응관계를 나타내지는 않았다. 각 실험군의 Endo III enzyme에 의한 tail length값의 분포를 살펴볼 때, 18, 20, 27, 33주간 노출하였을 때 대조군에 비해 노출군에서 tail length 값이 조금 높았지만, 양 반응 및 기간 반응관계를 보이지 않았고 수치의 크기가 불규칙하게 변화하였다. 즉, DNA에 있어 산화된 pyrimidine을 형성하여 손상된 부위의 염기를 제거함으로써 AP site (abasic site)를 만들고 이들이 Comet assay를 통해 break로 전환된 것을 포함한 DNA손상을 측정하기 위하여 endonuclease III (Endo III)를 첨가시킨 Endo III FLARE 분석법을 실시한 결과, 본 연구에서 나타난 결과는 용접흄 노출 영장류에서 Olive tail moment 및 tail length 공히 노출량 및 노출기간 반응관계를 볼 수 없었다. Endo III FLARE 분석법을 통한 산화적 DNA 손상지표는 영장류에 적용하기에는 적응반응현상으로 대조군과 유의한 차이도 관찰할 수 없었고 더욱이 역으로 대조군에서의 자연발생적 수치가 더 높아질 수 있어 용접흄 노출 영장류의 모니터링 지표로 사용하기에는 제한점이 있었다.

Effect of Bonding Temperature and Bonding Pressure on Deformation and Tensile Properties of Diffusion Bonded Joint of STS304 Compact Heat Exchanger (STS304 콤팩트 열교환기 고상확산접합부의 접합부 변형과 인장성질에 미치는 접합온도 및 접합압력의 영향)

  • Jeon, Ae-Jeong;Yoon, Tae-Jin;Kim, Sang-Ho;Kim, Hyeon-Jun;Kang, Chung-Yun
    • Journal of Welding and Joining
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    • v.32 no.4
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    • pp.46-54
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    • 2014
  • In this study, the effect of bonding temperature and bonding pressure on deformation and tensile properties of diffusion bonded joint of STS304 compact heat exchanger was investigated. The diffusion bonds were prepared at 700, 800 and $900^{\circ}C$ for 30, 60 and 90 min in pressure of 3, 5, and 7 MPa under high vacuum condition. The height deformation of joint decreased and the width deformation of joint increased with increasing bonding pressure at $900^{\circ}C$. The ratio of non-bonded layer and void observed in the joint decreased with increasing bonding temperature and bonding pressure. Three types of the fracture surface were observed after tensile test. The non-bonded layer was observed in diffusion bonded joint preformed at $700^{\circ}C$, the non-bonded layer and void were observed at $800^{\circ}C$. On the other hand, the ductile fracture occurred in diffusion bonded joint preformed at $900^{\circ}C$. Tensile load of joint bonded at $800^{\circ}C$ was proportional to length of bonded layer and tensile load of joint bonded at $900^{\circ}C$ was proportional to minimum width of pattern. The tensile strength of joint was same as base metal.

Mechanical Properties and Microstructure on Dissimilar Friction-Stir-Weld of Aluminium Alloys (FSW된 이종알루미늄합금의 접합 특성 및 미세 조직)

  • Han, Min-Su;Jang, Seok-Ki
    • Journal of Advanced Marine Engineering and Technology
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    • v.35 no.1
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    • pp.75-81
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    • 2011
  • Dissimilar joining of aluminum 6061-T6 alloy to aluminum 5083-O alloy was performed using friction-stir welding technique. The mechanical properties, hardness, macro- and micro-structure on dissimilar friction-stir-weld aluminium alloy were investigated. Mechanical properties of the weld mainly depend on which Al alloy is placed at the retreating sides of the rotating tool respectively during dissimilar friction-stir weld because the microstructure of stir zone was mainly composed of welded Al alloys of the retreating side. Onion ring pattern was observed like lamella structure stacked by each Al alloy in turn. It apparently results in defect-free weld zone that traverse speed was changed to 124 mm/min under conditions of tool rotation speed like 1250 rpm with 5 mm of tool's prove diameter, 4.5 mm of prove length, 20 mm of shoulder diameter, and $2^{\circ}$ of tilting angle. The 231 MPa of ultimate stress and the 121 MPa of yield point are obtained about the friction-stir-welded Al 6061-T6(AS) to Al 5083-O(RS).

An Analytical Study on the Improvement of the Performance of Swivel Valve Tube Couplers (스위벨 밸브 튜브 커플러 개발을 위한 해석 연구)

  • Lee, Jun-Ho;Sung, Jae-Kyeong
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.10 no.3
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    • pp.1-6
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    • 2011
  • This study focused on the localization of swivel type tube couplers, which all depend on imports. In this study, a computer application analysis was performed using a finite element method as a preliminary study. In the major developments related to the objective of this study, the air brake system produced by car makers represents a different in the installation point of an air tank according to the type of cars or in the length and direction of its hoses and that leads to cause lots of problems. For solving such problems, the design of the major elements in a swivel type tube coupler was analyzed using a finite element method, and its validity was also verified. In the process that verifies the validity of this study, it was necessary to investigate how much external force affects the desorption of the tube support, which is the most important element in swivel type tube couplers. For achieving the investigation, a pressure test was implemented for the tube support according to the Federal Motor Vehicle Safety Standards(FMVSS). In the results of the pressure test, all samples satisfied the FMVSS. In addition, several tests were implemented by installing the sample of the developed swivel type tube coupler to an actual vehicle. In particular, rotation tests with various angles were applied by welding the swivel type coupler to an air tank through an argon welding process. In the results of the installing test for an actual vehicle, it was verified that the designed structure was determined as a structure that is able to endure the eccentric torque and deformation pressure applied to several directions that are the major problems in such fixed type tube couplers. Therefore, in the comparison of the performance of the developed product with the product of PARKER, it was possible to verify that the localized swivel type tube coupler developed in this study shows more excellent than that of the existing products by PARKER.

A Study on the Stress Distribution and Stress Concentration of Pipe with Respect to Attached Shape and Method of the Bracket in a Welding Structure (브래킷 결합형식에 따른 용접 구조물의 파이프에서 발생하는 응력분포와 응력집중에 관한 연구)

  • Jeon, Hyung-Yong;Sung, Rak-Won;Han , Geun-Jo
    • Journal of the Korean Society for Precision Engineering
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    • v.16 no.8
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    • pp.28-37
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    • 1999
  • This investigation is the result of the structural analysis by finite element method and test for considering stress distribution and stress concentration to be generated according to the change of attached shape and method of the bracket to pipe in welding structure. Generally, members that consist structures are subjected to various forces and are jointed each other with a number of bracket. In this case, circular pipe was adapted in order to weld these members easily and to study the optimal design which is used a beam with shape section as main components of the structure, According to attached shape and method, distributed stress on circular pipe is appeared so differently. This may result deeply effects with respect to thickness, material properties. So a study on attaching shape and method of bracket to circular pipe is needed. In this paper, to obtain the maximum equivalent stress or stress concentration was used experimental and F.E.M. analysis. First five parameter was defined with respect to attached a shape and method to circular pipe i.e. the variation of the attached area, the variation of the attached shape, the variation of the attached length, the variation of both directin angles, the variation of the upper angle. Afterward the experimental analysis was practiced as the variation of the both direction angel and the finite element analysis was practiced as each parameters. We can discover stress distribution and stress concentration according to the change of form of bracket. And the result can be referenced for a design of similar structure.

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One Dimensional Thermal Elasto-Plastic Analysis Using Layered Beam Theory (적층보 이론을 이용한 1차원 열탄소성 해석)

  • S.I.,Seo;C.D.,Jang
    • Bulletin of the Society of Naval Architects of Korea
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    • v.27 no.4
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    • pp.51-57
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    • 1990
  • There exist residual stresses and deformations in welded structures because of nonuniform temperature distribution. The thermal elasto-plastic analysis is necessary to describe the behavor of the structure during welding. In this paper, we calculated the residual stresses and deformations of the welded beam using the I-dimensional layered beam theory. In the previous 1-dimensional analyses, there were restrictions that the equilibrium conditions which were effective only on beams with infinite length were used, and the boundary conditions could not be considered adequately. But, the layered beam theory based on the incremental finite element method, can overcome these restrictions. On the other hand, in the 2-dimensional analysis, the computing time is large because of many degrees of freedom, and there was inaccuracy in the calculation of welding deformations. However, the layered beam theory can take into account the variation of properties along the depth, and can reduce the degrees of freedom considerably in comparision with the 2-dimensional analysis, and shows good agreement with the experiments.

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The implementation of interface between industrial PC and PLC for multi-camera vision systems (멀티카메라 비전시스템을 위한 산업용 PC와 PLC간 제어 방법 개발)

  • Kim, Hyun Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.1
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    • pp.453-458
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    • 2016
  • One of the most common applications of machine vision is quality inspections in automated production. In this study, a welding inspection system that is controlled by a PC and a PLC equipped with a multi-camera setup was developed. The system was designed to measure the primary dimensions, such as the length and width of the welding areas. The TCP/IP protocols and multi-threading techniques were used for parallel control of the optical components and physical distribution. A coaxial light was used to maintain uniform lighting conditions and enhance the image quality of the weld areas. The core image processing system was established through a combination of various algorithms from the OpenCV library. The proposed vision inspection system was fully validated for an actual weld production line and was shown to satisfy the functional and performance requirements.

Fatigue Evaluation of a Steel Bridge in Service through Stress History Measurement and Consideration of Stress Category (공용중인 교량의 응력이력 계측 및 응력범주를 고려한 피로평가)

  • Na, Sung-Ok;Kwon, Min-Ho;Cha, Cheol-Jun;Kim, In-Ho
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.18 no.2
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    • pp.108-116
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    • 2014
  • The proper stress history measurement should be conducted in order to examine the accurate cause of fatigue cracks or the fatigue safety in the steel bridge. Only one strain gauge is generally installed in the field for the stress history examination because of the field circumstances, economic feasibility, workability, and so on. However, this method may not consider the actual size of the specific structure, the gauge length, and the affect of stress concentration in the welded joint. In addition, it is difficult to apply for the stress analysis. Therefore, this study suggests improvements that are a great number of gauge installations, the gauge location adjustment, and the use of the minimum length gauge. It is drived the correlative equation of strain for the distance between the welding toe and the strain gauge installation, and compare correlative equation with equation of IIW. Also, this study could estimate the remaining life and fatigue damage of bridge in service by selecting the suitable stress category. In conclusion, it is possible to understand the member which is high in the fatigue cracks, and the quantitative relations between the welding toe and the strain gauge installation distances. The proposed approach in this study can make an more accurate fatigue damage and a remaining life prediction so that the improved method should be applied in measuring the strain of bridges from now on.

Evaluation of Buckling Load and Specified Compression Strength of Welded Built-up H-section Compression Members with Residual Stresses (잔류응력의 영향을 고려한 조립 H-형강 부재의 좌굴하중 및 설계압축강도 평가)

  • Lee, Soo-Keuon;Yang, Jae-Guen;Kang, Ji-Seok
    • Journal of Korean Society of Steel Construction
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    • v.29 no.1
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    • pp.81-88
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    • 2017
  • Residual stress is defined as stress that already exists on a structural member from the effects of welding and plastic deformation before the application of loading. Due to such residual stress, welded H-section compression members under centroidal compression load can undergo buckling and failure for strength values smaller than the predicted buckling load and specified compressive strength. Therefore, this study was carried out to evaluate the effect of residual stress from welding on the determination of the buckling load and specified compressive strength of the H-section compression member according to the column length variation. A three-dimensional nonlinear finite element analysis was performed for the H-section compression member where the welded joint was fillet welded by applying heat inputs of 3.1kJ/mm and 3.6kJ/mm using the SAW welding method.

A Study on the Snap-through Behaviour of Plate Elements due to the Initial Deflection Shape (초기처짐형상에 따른 판부재의 천이거동에 관한 연구)

  • Park, Joo-Shin;Lee, Kye-Hee;Ko, Jae-Yong
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.18 no.1
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    • pp.13-20
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    • 2005
  • Since High Tensile Steel has been widely used to thin plate on the steel structure and marine structure, It has increased possibility of buckling. Especially, initial deflection of ship structure is mainly caused by heat processing of welding or cutting etc. This initial deflection has negative effect to thin plate, which would incur a complicated nonlinear behavior accompanied with secondary buckling. If idealized initial deflection is considered in early marine structure design of secondary buckling, accuracy and reliability will be improved considerably. The measurement data of initial deflection from experiment is applied to finite element series analysis. For FEA(ANSYS), Applied nonlinear buckling analysis is used by Newton-Raphson method & Arc-length method included in this program.