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Agronomic Traits of Soybean Breeding Lines with Low Stachyose and Raffinose Contents (Stachyose 및 Raffinose 저함량 콩 선발계통의 농업적 형질)

  • Ha, Do Su;Moon, Jin Young;Choi, Sang Woo;Shim, Sang In;Kim, Min Chul;Chung, Jong Il
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.2
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    • pp.143-148
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    • 2017
  • Soybean [Glycine max (L.) Merr.] seed is an important dietary source of protein, oil, carbohydrates, isoflavones, and other nutrients for humans and animals. Raffinose and stachyose are the main antinutritional factors in soybean seed. They are carbohydrates belonging to the raffinose family of oligosaccharides, which are not readily digested in humans and cause flatulence or diarrhea. The genetic reduction of the raffinose and stachyose contents in mature soybean seeds will improve the nutritional value of soybean. The objective of this research was to evaluate agronomic traits with 10 $F_6$ strains selected from breeding populations derived from a cross among seven parents. The contents of raffinose and stachyose in mature seeds were detected by high-performance liquid chromatography. Agronomic traits such as flower color, flowering date, harvesting date, lodging, plant height, seed coat color, hilum color, 100 seed weight, and yield were evaluated. Ten intermediate parents showed low raffinose and stachyose contents. The intermediate parent 883-1 had a small seed size, six intermediate parents (15A1, 15D1, RS-5, RS-33, RS-64, and RS-70) had a medium seed size, and two intermediate parents (14G20 and RS-21) had a large seed size. The intermediate parent RS-21 had a black seed coat and a green cotyledon. Four intermediate parents (883-1, 14G20, RS-5, and RS-21) had elite agronomic traits. The new intermediate parents developed through this study will be used to develop improved soybean cultivars with low contents of raffinose and stachyose.

Effect of Dietary Silkworm Droppings on Chemical Composition of Pork Loin (잠분(蠶糞) 급여가 돈육 등심의 화학적 조성에 미치는 영향)

  • Lee, J.I.;Lee, J.D.;Ha, Y.J.;Jung, J,D.;Lee, J.W.;Lee, J.R.;Kwack, S.J.;Kim, D.H.;Do, C. H.
    • Journal of Animal Science and Technology
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    • v.46 no.6
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    • pp.1013-1022
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    • 2004
  • This study investigated the effects of silkworm droppings(SWD) added diet feeding on chemical composition of pork loin meat. Pigs were divided into 7 treatment groups(10 pigs/group) and subjected to one of seven treatment diets(O, 1 0/0, 2 0/0, 3% SWD for 4 weeks, 1 0/0, 2 0/0, 3% SWD for 8 weeks, SWD diets; total fed diets) before slaughter. Pork loin were collected from the animals(1l0 kg body weight) slaughtered at a commercial slaughter house. Pork loin meat were aerobic packaged and then stored at$4^{\circ}C$ for 2, 5, 8, or 12 days. Samples were analyzed for TBARS, sensory evaluation, myoglobin content, fatty acid composition, amino acid and mineral content. TBARS value was not significantly different between the control and SWD treatment groups. Myoglobin content of SWD fed group pork was significantly increased than that of control group(P<0.05). Marbling score and acceptability were increased in SWD fed pork compared to those in control pork. Na, Mg, Ca, Mn and Fe content of SWD fed group pork was significantly decreased than those of control group(P<0.05). P content was significantly increased than that of control group(P< 0.05). In the change of amino acid composition, the contents of aspartic acid, serine and tyrosine were decreased by dietary SWD-supplementation, whereas the SWD-supplementation resulted in the higher glutamic acid, alanine and isoleucine. As dietary SWD was increased in feed, the content of oleic acid was significantly increased, but stearic acid was significantly decreased(P<0.05).

Determination of Crude Protein Requirements for Maintenance of Prepubertal Hanwoo Heifers (한우 암송아지의 성성숙 전 유지 단백질 요구량 결정)

  • Nam, In-Sik;Oh, Young-Kyoon;Jang, Sun-Sik;Kim, Do-Hyung;Seol, Yong-Joo;Kim, Kyoung-Hoon
    • Journal of Animal Science and Technology
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    • v.53 no.2
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    • pp.171-176
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    • 2011
  • Two trials at different body weights of Hanwoo heifers (average body weight of 143 and 257 kg, respectively) were conducted to determine crude protein requirements for maintenance (CPm). Six Hanwoo heifers in each trial were used in two 3 ${\times}$ 3 Latin square design with three diets containing three levels of CP, 14 days in each period. In trial 1, the diets were based on 2.8 kg fresh wt./day/heifer timothy hay (LCP) with supplements of either 250 g ground corn and 150 g corn gluten meal (MCP) or 500 g ground corn and 300 g corn gluten meal (HCP). In trial 2, the diets were based on 4.8 kg fresh wt./day/heifer timothy hay (LCP) with supplements of either 350 g ground corn and 250 g corn gluten meal (MCP) or 700 g ground corn and 500 g corn gluten meal (HCP). In trial 1, CP intakes were 236.6, 340.1, and 459.8 g/d for LCP, MCP, and HCP, respectively. Crude protein balances were 0.51, 1.87 and 3.20g/$BW^{0.75}$/d for LCP, MCP, and HCP, respectively. In trial 2, CP intakes were 415.2, 606.9 and 793.0g/d for LCP, MCP and HCP, respectively. Crude protein balances were 0.67, 1.03, 2.99 g/$BW^{0.75}$/d for LCP, MCP, and HCP, respectively. The maintenance requirements for CP from the regression equation between CP intake and CP balance were 4.58g/$BW^{0.75}$/d (trial 1) and 5.02 g/$BW^{0.75}$/d (trial 2) and lower than the value (5.56 g/$BW^{0.75}$/d) adopted by Korean Feeding Standards for Hanwoo (2007).

Influences of Worm Casting Organic Fertilizer on Weed Invasion on the Creeping Bentgrass Sward (지렁이분 시비가 잡초의 침입과 벤트그라스 잔디초지에 미치는 영향)

  • Park Sung-Jun;Cho Nam-Ki;Kang Young-Kil;Song Chang-Khil;Hyun Hae Nam;Cho Young-Il
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.3
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    • pp.211-216
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    • 2005
  • This study was conducted from March 21 to July 10 in 2004 at JeJu to investigate the influences of worm casting organic fertilizer rates (0, 150, 300, 450, 600 kg/10a) on creeping bentgrass sward. The result obtained were summarized as follows; plant height was getting longer as organic fertilizer increased from 0 to 600 kg/10a. But it was no significance from 450 to 600kg/10a. Root length, SPAD reading value, leave and root weight were the same trend with plant height response. Percentage of land cover and density of creeping bentgrass increased as fertilizer rate increased from 0 to 600 kg/10a. But percentage of land cover and density of weed decreased. Number of weed species were decreased as increasing of organic fertilizers. Then ranking of the dominant weeds were Chenopodium album var. cetrorubrum and Digitaria adscendens, Polygonum hydropiper (at 0kg/10a), Portulaca oleracea and Digitaria adscendens, Polygonum hydropiper (at 150kg/10a), Polygonum hydropiper and Poa annua, Digitaria adscendens (at 450 kg/10a), Polygonum hydropipr and Digitaria adscendens, Portulaca oleracea (at 600 kg/10a).

Effect of Sand Particle Sizes on Turf Vegetation of Creeping Bentgrass (모래입경이 Creeping Bentgrass 잔디 초지의 식생에 미치는 영향)

  • Park Sung-Jun;Cho Nam-Ki;Kang Young-Kil;Song Chang-Khil;Cho Young-Il
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.3
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    • pp.205-210
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    • 2005
  • This study was conducted from March 21 to July 9 in 2004 at JeJu Island to investigate the effect of different particle sizes (0.3-0.5, 0.5-0.8, 0.8-1.0, 1.0-1.5 and 1.5-2.0mm) on creeping bentgrass vegetation. The results obtained were summarized as follows; plant height became shorter as particle size was increased from 0.3-0.5 to 1.5-2.0 n. Root length, Minolta SPAD-502 chlorophyll reading value, leave and root weight were directly proportional plant height response. Degree of land cover and density of creeping bentgrass decreased as the particle size was increased from 0.3-0.5 to 1.5-2.0nm, and degree land cover and density of weed increased. The number of weed species were increased as the sand particle size was increased. Then ranking of the dominant weeds were Portulaca oleracea, Trifolium repens and Cyperus amuricus (at 0.3-0.5 and 0.5-0.8mm particle size), Trifolium repens, Portulaca oleracea and Polygonum hydropiper (at 0.8-1.0mm particle size), Portulaca oleracea, Polygonum hydropiper and Poa annua (at 1.5-2.0mm particle size). Based on the these findings, the optimum sand particle size for growth of creeping bentgrass seems to be about 0.3-0.5m in volcanic ash soils of Jeju island.

Effect of Cycloheximide and Holding Solution on Vase Life of Cut 'Blue Magic' Iris Flowers according to the Flower Development and Opening Stages (Cycloheximide 및 보존용액 처리가 개화단계에 따른 절화 아이리스 '블루매직'의 수명에 미치는 영향)

  • Kim, Young-A;Lee, Jong-Suk
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.790-795
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    • 2010
  • This experiment was conducted to clarify the effects of cycloheximide and holding solution on vase life of cut 'Blue Magic' iris. The vase life of iris flowers held in 3% sucrose (S) + $100mg{\cdot}L^{-1}$ hydroxy quinoline sulfate (HQS) + $50mg{\cdot}L^{-1}$ $AgNO_3$ + $100mg{\cdot}L^{-1}$ Benzylaminopurine (BA), 3% S + $100mg{\cdot}L^{-1}$ HQS + $10{\mu}M$ cycloheximide (CHI), or 3% S + $100mg{\cdot}L^{-1}$ HQS + $50{\mu}M$ CHI were much longer than those held in distilled water. Squeeze stem phenomenon that showed at a holding solution containing $200mg{\cdot}L^{-1}$ HQS disappeared at a holding solution containing $100mg{\cdot}L^{-1}$ HQS. The holding solution containing 3% S + $100mg{\cdot}L^{-1}$ HQS + $50mg{\cdot}L^{-1}$ $AgNO_3$ + $100mg{\cdot}L^{-1}$ BA extended the most effective treatments on vase life, fresh weight, water balance, and flowering of cut iris flowers. However, the holding solution containing 3% S + $100mg{\cdot}L^{-1}$ HQS + $10{\mu}M$ CHI and 3% S + $100mg{\cdot}L^{-1}$ HQS + $50{\mu}M$ CHI was not effective in solution uptake or transpiration, but did result in high water balance. Iris flowers treated with CHI at the half-open flower stage showed increases in ornamental value, such as full flower opening and extended vase life. To improve flower quality and prolonging vase life of cut iris flowers, a holding solution containing $50{\mu}M$ CHI can be used continuously from the half-open stage.

Preparation of TiO2-SiO2 Powder by Modified Sol-Gel Method and their Photocatalytic Activities (수식 졸-겔법에 의한 TiO2-SiO2분체합성 및 광촉매활성)

  • Kim, Byung-Kwan;Mizuno, Noritaka;Yasui, Itaru
    • Applied Chemistry for Engineering
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    • v.7 no.6
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    • pp.1034-1042
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    • 1996
  • Various $TiO_2-SiO_2$ composite powders were prepared by the modified sol-gel method using 1-dodecanol as DCCA (Dryng Control Chemical Additive ). Their characterizations were carried out and their photocatalytic catalysis was examined on the evolution reaction of hydrogen. The weight losses at $500^{\circ}C$ of only $TiO_2$ and $SiO_2$ powders were 33. 0wt% and 42.5wt%, respectively, and those of the $TiO_2/SiO_2$ powders ($TiO_2/SiO_2=25/75$, 50/50 and 75/25) were about $70.0{\pm}3.0wt%$. The released substances from the powders were almost organic matters. The as-prepared powders except only $TiO_2$ powder were amorphous. Transformation of anatase to rutil was hindered by $SiO_2$ component and the crystallinity of anatase was decreased with increasing $SiO_2$ contents. The as-prepared powders were bulky states. By heating at $600^{\circ}C$ for 1 hr $TiO_2-SiO_2$ powders ($TiO_2=100%$, $TiO_2/SiO_2=75/25,\;50/50$) showed agglomerates consisted of particles in submicron, but those of $TiO_2/SiO_2=25/75$ and $SiO_2=100%$ were still bulky states. Specific surface area of the powders heat-treated at $600^{\circ}C$ for 1hr was increased with $SiO_2$ concents and their pore sizes were also depended on $SiO_2$ contents. The photocatalytic activity of $TiO_2/SiO_2=75/25$ heat-treated at $600^{\circ}C$ for 1hr was 0.240mo1/h.g-cat as $H_2$ evolution rate. This value was about 2.0 times that of P-25(Degussa P-25) as a standard photocatalyst.

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Characteristic Properties of Fucoidan Sulfate Purified from Gompi, Ecklonia stolonifera (곰피에서 정제한 Fucoidan Sulfate의 특성)

  • Lee, Hong-Soo;Jin, Sung-Hyun;Kim, Hee-Sook;Ryu, Byung-Ho
    • Korean Journal of Food Science and Technology
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    • v.27 no.5
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    • pp.716-723
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    • 1995
  • The fucoidan purified from Korean brown seaweed, Ecklonia stolonifera was characterized on molecular structure and blood anticoagulant activities. Extraction was conducted at $100^{\circ}C$ with water and repeated twice. The crude fucodian was 151.1g out of 20.0 kg of Ecklonia stolonifera. The Fucoidan-1, which was purified from crude fucoidan using calcium chloride and cetyl pyridium chloride (CPC), was 35.2% against crude fucoidan. Fucoidan-5 was obtained approximately 28.1% from Fucoidan-1 through DEAE-Toyopearl 650 M ion-exchange column chromatography and showed one band by cellulose acetate electrophoresis. The molecular weight of Fucoidan-5 was estimated to be about 21,000∼23,000 dalton by Sephacryl S-300 gel filtration chromatography. Fucoidan-5 consists of 35.7% of fucose and 4.3% of galactose and the molar ratio of fucose and sulfate was about one to one. IR spectrum of Fucoidan-5 showed absorption at $1240\;cm^{-1}\;and\;850\;cm^{-1}$ and specific rotation value, $[\alpha]$, was $[\alpha]$. These results suggests that the sulfate maybe bind at $C_{4}$ carbon on ${\alpha}-L-fucose$. Gas chromatograph of methyl alditol acetate revealed that Fucoidan-5 is a fucose containing sulfated polysaccharide with $({\alpha}l-2)\;or\;({\alpha}l-2)$ glycosidic linkage. Anti-thrombin activity of the Fucoidan-5 was estimated as 1.4 time stronger than heparin. From above results, the purification methods using CPC and ion exchange chromatography is effective tools for obtaining highly purified fucoidan from Gompi, Ecklonia stolonifera.

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The Consumption Status and Preference for Sprouts and Leafy Vegetables (새싹채소 및 쌈채소의 선호도 및 소비실태)

  • Jun, So-Yun;Kim, Tae-Hun;Hwang, Sung-Heon
    • Food Science and Preservation
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    • v.19 no.5
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    • pp.783-791
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    • 2012
  • The purpose of the study was to provide baseline information that could encourage consumers and establish the direction of development for sprouts and leafy vegetables. A survey on the consumption and preference for sprouts and leafy vegetables of 823 people over 20 years of age in Seoul and Gyeonggi province was conducted. The preference for sprouts was higher in men ($3.75{\pm}0.96$) than in women ($3.64{\pm}0.84$), whereas the preference for leafy vegetables was higher in women ($4.06{\pm}0.74$) than in men ($3.88{\pm}0.90$). Based on age, the respondents in their 40s or above had high preferences for sprouts and leaf vegetables due to the importance that they accorded to health and nutrition (p<0.01). The sprouts and leafy vegetables were purchased mainly for their high nutritional value. The purchase frequency of once a month or more for leafy vegetables (82.7%) was higher than that for sprouts (57.1%). The consumers purchased sprouts and leafy vegetables mainly from a large discount store. The respondents in their 20s showed the highest percentage of purchasing from a local store whereas the older ones showed a high preference for traditional markets. The reason for the choice of the place of purchase was convenience. The freshness and price were important factors in the consumption of sprouts and leafy vegetables. In the case of leafy vegetables, the consumers preferred to buy them using the conventional weight measurement system rather than in small packages. On the other hand, mixed small packages were preferred for sprouts. The purchase frequency was correlated with the preferences, need for improvement and purchase intent for sprouts and leafy vegetables. The aspects that require improvement for producing high-quality sprouts and leafy vegetables were the nutritional and functional properties. To increase the consumption of sprouts and leafy vegetables, there is need for variations and improvement of the quality and safety of the products.

숙성 토하젓의 기능성분 및 토하젓 소스 개발에 관한 연구

  • 박복희
    • Proceedings of the SOHE Conference
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    • 1997.12a
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    • pp.39-43
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    • 1997
  • Toha-jeod was manufactured by seven methods ; low salt group (L:15% sodium chloride), high salt g group (H:23% sodium chloride), 50% conventional soybean sauce group (S), low salt group containing 2% w wheat bran (W2%-L), high saIt group containing 2% wheat bran (W2%-H),high salt group containing 2% wheat bran (W2%-H), high salt group containing 4% wheat bran (W4%-H). After these seven groups were refrigerated at $4{\pm}1^{\circ}C$, they were sampled at intervals of three months and analyzed functional components. The free amino acid in Toha-jeod which are omitine, glutamic acid, leucine, alanine, lysine and valine increased gradually up to six months of fermentation and decreased by nine months. Conventional soybean sauce group increased continuously during the fermentation process. Hypoxanthine was altered almost among other nueletides. ATP was not detected, IMP and inosine had disapapted after the six months fermentation. Polyene fatty acids and n-3 fatty acids were decreased and s saturated fatty acids were not altered in the group containing wheat bran during fermentation. In the Hunter values, the group containing wheat bran and high salt group showed lower level than the group n not containing wheat bran and low salt group. Redness indicating the value of Toha-jeod increased as Toha-jeod was fermentated. Low salt group and conventional soybean sauce group were superior to other groups in the extent of redness. As the fermentation of Toha-jeod progressed for a long time, molecular weight distribution tended to become less molecular and the formation of chitin oligosaccharides was increased significantly. After nine months of fermentation, 24.75% chitin oligosaccharides [($GlcNAd_4$ ~ ($GlcNAd_8$, M.W. 823~1789] were created in the high salt group containing 2% wheat bran. [($GlcNAd_6$. M.W. 1236J , that is NACOS-6, which was reported as an antitumor activity material, was present in 4.01~4.37% of total Toha chitin content. 66.30% chitin oligosaccharides were created in conventional soybean sauce.

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