• 생명과학회지
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• 제18권1호
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• pp.114-119
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• 2008

Wee1 인산화효소는 세포주기 조절의 핵심 단백질인 cdc2/cyclinB 복합체를 인산화하여 활성을 억제, 조절하는 주요한 효소이다. 지금까지 포유동물에서는 Wee1A, Wee1B 그리고 Myt1의 세 가지 효소가 발견되었다. Wee1 인산화효소의 조절기작을 연구하기 위하여 생쥐의 Wee1A와 Wee1B를 발톱개구리의 난자에 주사한 후 단백질의 변형을 관찰하였다. 이 세포주기 과정에서 두 효소는 모두 인산화 되었으며, Wee1A단백질은 분해되는 것을 관찰 할 수 있었다. 또한 세포외 인산화 방법을 통하여 Wee1A가 PKA와 Akt에 의해 인산화됨을 확인하였다. 이러한 Wee1 인산화효소의 인산화와 단백질 안정성에 영향을 미치는 단백질 내의 부위를 살펴보고자, Wee1A와 Wee1B의 아미노 도메인과 카르복실 도메인을 서로 치환한 단백질을 제조하여 개구리 난자에 주사하고 인산화 정도와 단백질의 안정성을 조사하였을 때, Wee1A의 아미노 도메인이 단백질의 인산화와 안정화에 중요한 영향을 미친다는 것을 규명하였다. 그리고 Wee1B의 아미노 도메인과 Wee1A의 카르복실 도메인은 효소의 활성을 조절하는 역할을 한다.

• 대한임상검사과학회지
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• 제38권1호
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• pp.72-81
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• 2006

Wee1 is a kinase regulator of the M-phase promoting factor (MPF; a complex of cdc2 and cyclin B1). The present study was undertaken to determine the role(s) of wee1 in the early stages of mouse ovarian follicles. The expression of wee1 and the correlated cell-cycle components, namely cdc2, cyclin B1, and cdc25C, were evaluated by immunohistochemistry. In addition, the expression of Tyr15-phosphorylated cdc2 (cdc2-p) was also examined to determine whether wee1 kinase phosphorylates cdc2 existed. Each component except cdc25C was found cytoplasmic in the oocytes at all stages of follicles, while cdc25C was not detected in primordial follicles. It was found primarily in ovarian somatic cells and to a small extent in granulosa cells of the growing follicles. To further confirm the expression of cell-cycle components in the primordial follicular oocytes, day1 ovaries were enzymatically and mechanically dissociated, then oocytes were isolated from somatic including pre-granulosa cells, and we confirmed that cdc2-p was expressed in oocytes of primordial follicles. From the results of the present study, we concluded wee1, without the counteracting cdc25C, would cause meiotic arrest of oocytes by the inhibitory phosphorylation of cdc2. The expression of all these proteins in the granulosa cells of growing follicles may regulate their mitosis concurrently with the growth of oocytes and follicles.

• 충청수학회지
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• 제18권1호
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• pp.41-49
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• 2005

In this paper, we prove the generalized Hyers-Ulam- Rassias stability of the functional equation $$af(\frac{x+y+z}{b})+af(\frac{x-y+z}{b})+af(\frac{x+y-z}{b})+af(\frac{-x+y+z}{b})=cf(x)+cf(y)+cf(z)$$.

• Journal of the Korean Statistical Society
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• 제12권2호
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• pp.100-109
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• 1983

Let ${X_i}$ be a process with stationary and independent increments whose log characteristic function is expressed as $ibut-2^{-1}\sigma^2u^2t+t\int_{{0 }^c}{(exp(iux)-1-iux(i+x^2)^{-1})dv(x)}$. Our main result is taht $x^2(\int_{\y\>x}{dv(y)})/(\int_{$\mid$y$\mid$\leqx}{y^2dv(y)+\sigma^2}) \to 1$ as $x \to 0 (resp. x \to \infty)$ is necessary, and sufficient for ${X-i}$ to have ${A_t}$ and ${B_t}$ such that $(X_t-A_t)/B_t \to^D n(0,1)$ as $t \to 0 (resp. t \to \infty)$.

• BMB Reports
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• 제46권6호
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• pp.289-294
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• 2013

To maintain cellular homeostasis against the demands of the extracellular environment, a precise regulation of kinases and phosphatases is essential. In cell cycle regulation mechanisms, activation of the cyclin-dependent kinase (CDK1) and cyclin B complex (CDK1:cyclin B) causes a remarkable change in protein phosphorylation. Activation of CDK1:cyclin B is regulated by two auto-amplification loops-CDK1:cyclin B activates Cdc25, its own activating phosphatase, and inhibits Wee1, its own inhibiting kinase. Recent biological evidence has revealed that the inhibition of its counteracting phosphatase activity also occurs, and it is parallel to CDK1:cyclin B activation during mitosis. Phosphatase regulation of mitotic kinases and their substrates is essential to ensure that the progression of the cell cycle is ordered. Outlining how the mutual control of kinases and phosphatases governs the localization and timing of cell division will give us a new understanding about cell cycle regulation.

• Journal of Radiation Protection and Research
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• 제6권1호
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• pp.31-33
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• 1981

The 42 patients with carcinoma of the cervix, performed intracavitary radiotherapy, were analysed the doses at points A and B comparing to the mghr prescription. The doses at points A and B were calculated by PC-12 computer planning system. Correlation coefficienty between doses at points A and B and the mghr prescription are 0.82 (p<0.001) and 0.90 (p<0.001) respectively. The slope of the point A line is 0.70 and the slope of the point B is 0.21. Therefore, the dose at point A is approximately 3/4 the mghr prescription and the dose at point B is approximately 1/4 the mghr precription.

• 천문학회지
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• 제29권spc1호
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• pp.145-146
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• 1996

We have estimated a metal abundance of [Fe/H]= -0.04 $\pm$ 0.05 dex, a reddening of E(B- V)= 0.28 mag, an age of 1.1 $\pm$ 0.1 Gyr, and a distance of 2.5 $\pm$ 0.2 kpc for NGC 1245 using the Washington filter system.

• 호남수학학술지
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• 제26권1호
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• pp.61-75
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• 2004

It is shown that every almost linear mapping $h:{\mathcal{A}}{\rightarrow}{\mathcal{B}}$ of a unital Poisson Banach algebra ${\mathcal{A}}$ to a unital Poisson Banach algebra ${\mathcal{B}}$ is a Poisson algebra homomorphism when h(xy) = h(x)h(y) holds for all $x,y{\in}\;{\mathcal{A}}$, and that every almost linear almost multiplicative mapping $h:{\mathcal{A}}{\rightarrow}{\mathcal{B}}$ is a Poisson algebra homomorphism when h(qx) = qh(x) for all $x\;{\in}\;{\mathcal{A}}$. Here the number q is in the functional equation given in the almost linear almost multiplicative mapping. We prove that every almost Poisson bracket $B:{\mathcal{A}}\;{\times}\;{\mathcal{A}}\;{\rightarrow}\;{\mathcal{A}}$ on a Banach algebra ${\mathcal{A}}$ is a Poisson bracket when B(qx, z) = B(x, qz) = qB(x, z) for all $x,z{\in}\;{\mathcal{A}}$. Here the number q is in the functional equation given in the almost Poisson bracket.

• 한국가구학회지
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• 제14권1호
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• pp.47-58
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• 2003

Italian furniture companies play a leading role in the world furniture market and are acknowledged as good design trend leader. Their craftsmanship which has been handed down from the past is thoroughly applied to the process of design and production. Two well-known furniture companies in Italy, 'B&B Italia' and 'Kartell', are chosen as a case study model to grasp the key of their success. In general and objective material collecting and researching, this study aims at understanding the method of the advanced corporations' design development, marketing strategy and brand identity This study is expected to use as a research material for Korean furniture industry to escape from the serious depression after IMF shock.

• 생명과학회지
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• 제16권5호
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• pp.828-833
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• 2006

옥수수 (Zea mays L.) 꽃은 암술 세포사멸과 수술 세포 성장정지 등을 통하여 양성상태에서 단성 상태로 성결정 과정을 완성한다. 본 논문에서는 옥수수 성 결정 동안 세포주기 유전자들의 시간적, 공간적 발현조절을 조사하였다. 세포주기의 양성조절 인자 즉 cyclin A, cyclin B, cyclin dependent kinase A (CDK A), Mad2 유전자들은 성장하는 암술과 수술에서 높게 발현되는 반면 죽어가는 암술과 성장이 정지되는 수술에서는 이들의 발현이 사라졌다. 이와 반대로, Wee1과 CDK inhibitor (CKI) 같은 세포주기 음성 조절유전자들은 야생형 암꽃과 tasselseed2 돌연변이 수꽃의 성장이 정지하고 있는 수술에서 발현이 증가되었지만, 흥미롭게도, 이들 유전자들은 죽어가는 암술세포에서는 발현되지 않았다. 이들 결과들을 통하여 옥수수 성 결정 과정 중에서 암술 세포사멸과 수술세포 성장정지는 세포주기조절과 밀접한 관계가 있으며, 특히 성장이 정지하는 수술과 죽어가는 암술에서의 음성 세포주기 조절 유전자들의 다른 발현양상은 이 둘의 성 결정 메커니즘이 구별 될 것이라고 사료된다.