• Journal of Life Science
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• v.18 no.1
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• pp.114-119
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• 2008

The molecular machinery controlling cell cycle is centered around the regulation of the activity of maturation-promoting factor (MPF), a complex composed of a catalytic Cdc2 and the cyclinB regulatory subunit. Cdc2 kinase is inactivated by phosphorylation of inhibitory kinase, Wee1. It has been known that there are three different Wee1 kinases in the mammalian cell, Wee1A, Wee1B and Myt1. To investigate the regulatory mechanism of Wee1 kinases, the phosphorylation and degradation of Wee1A and Wee1B were checked in the Xenopus oocyte cell cycle. When Wee1 kinases were injected into frog oocyte, Wee1B was more stable than Wee1A. Wee1A and Wee1B kinase were phosphorylated by many kinases such as PKA and Akt. The roles of amino or carboxyl terminal in mouse Wee1A or Wee1B kinase were investigated using chimeric constructs. The degree of protein phosphorylation, degradation and cell cycle progression were different between chimeric constructs. The amino domain of Wee1A was implicated in the protein phosphorylation and degradation while amino domain of Wee1B and carboxyl domain of Wee1A were involved in the activity regulation. These results suggested that the domains of Wee1 kinase have different and significant roles in regulating the Wee1 kinases in the cell cycle progression.

• Korean Journal of Clinical Laboratory Science
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• v.38 no.1
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• pp.72-81
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• 2006

Wee1 is a kinase regulator of the M-phase promoting factor (MPF; a complex of cdc2 and cyclin B1). The present study was undertaken to determine the role(s) of wee1 in the early stages of mouse ovarian follicles. The expression of wee1 and the correlated cell-cycle components, namely cdc2, cyclin B1, and cdc25C, were evaluated by immunohistochemistry. In addition, the expression of Tyr15-phosphorylated cdc2 (cdc2-p) was also examined to determine whether wee1 kinase phosphorylates cdc2 existed. Each component except cdc25C was found cytoplasmic in the oocytes at all stages of follicles, while cdc25C was not detected in primordial follicles. It was found primarily in ovarian somatic cells and to a small extent in granulosa cells of the growing follicles. To further confirm the expression of cell-cycle components in the primordial follicular oocytes, day1 ovaries were enzymatically and mechanically dissociated, then oocytes were isolated from somatic including pre-granulosa cells, and we confirmed that cdc2-p was expressed in oocytes of primordial follicles. From the results of the present study, we concluded wee1, without the counteracting cdc25C, would cause meiotic arrest of oocytes by the inhibitory phosphorylation of cdc2. The expression of all these proteins in the granulosa cells of growing follicles may regulate their mitosis concurrently with the growth of oocytes and follicles.

• Journal of the Chungcheong Mathematical Society
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• v.18 no.1
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• pp.41-49
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• 2005

In this paper, we prove the generalized Hyers-Ulam- Rassias stability of the functional equation $$af(\frac{x+y+z}{b})+af(\frac{x-y+z}{b})+af(\frac{x+y-z}{b})+af(\frac{-x+y+z}{b})=cf(x)+cf(y)+cf(z)$$.

• Journal of the Korean Statistical Society
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• v.12 no.2
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• pp.100-109
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• 1983

Let ${X_i}$ be a process with stationary and independent increments whose log characteristic function is expressed as $ibut-2^{-1}\sigma^2u^2t+t\int_{{0 }^c}{(exp(iux)-1-iux(i+x^2)^{-1})dv(x)}$. Our main result is taht $x^2(\int_{\y\>x}{dv(y)})/(\int_{$\mid$y$\mid$\leqx}{y^2dv(y)+\sigma^2}) \to 1$ as $x \to 0 (resp. x \to \infty)$ is necessary, and sufficient for ${X-i}$ to have ${A_t}$ and ${B_t}$ such that $(X_t-A_t)/B_t \to^D n(0,1)$ as $t \to 0 (resp. t \to \infty)$.

• BMB Reports
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• v.46 no.6
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• pp.289-294
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• 2013

To maintain cellular homeostasis against the demands of the extracellular environment, a precise regulation of kinases and phosphatases is essential. In cell cycle regulation mechanisms, activation of the cyclin-dependent kinase (CDK1) and cyclin B complex (CDK1:cyclin B) causes a remarkable change in protein phosphorylation. Activation of CDK1:cyclin B is regulated by two auto-amplification loops-CDK1:cyclin B activates Cdc25, its own activating phosphatase, and inhibits Wee1, its own inhibiting kinase. Recent biological evidence has revealed that the inhibition of its counteracting phosphatase activity also occurs, and it is parallel to CDK1:cyclin B activation during mitosis. Phosphatase regulation of mitotic kinases and their substrates is essential to ensure that the progression of the cell cycle is ordered. Outlining how the mutual control of kinases and phosphatases governs the localization and timing of cell division will give us a new understanding about cell cycle regulation.

• Journal of Radiation Protection and Research
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• v.6 no.1
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• pp.31-33
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• 1981

The 42 patients with carcinoma of the cervix, performed intracavitary radiotherapy, were analysed the doses at points A and B comparing to the mghr prescription. The doses at points A and B were calculated by PC-12 computer planning system. Correlation coefficienty between doses at points A and B and the mghr prescription are 0.82 (p<0.001) and 0.90 (p<0.001) respectively. The slope of the point A line is 0.70 and the slope of the point B is 0.21. Therefore, the dose at point A is approximately 3/4 the mghr prescription and the dose at point B is approximately 1/4 the mghr precription.

• Journal of The Korean Astronomical Society
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• v.29 no.spc1
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• pp.145-146
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• 1996

We have estimated a metal abundance of [Fe/H]= -0.04 $\pm$ 0.05 dex, a reddening of E(B- V)= 0.28 mag, an age of 1.1 $\pm$ 0.1 Gyr, and a distance of 2.5 $\pm$ 0.2 kpc for NGC 1245 using the Washington filter system.

• Honam Mathematical Journal
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• v.26 no.1
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• pp.61-75
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• 2004

It is shown that every almost linear mapping $h:{\mathcal{A}}{\rightarrow}{\mathcal{B}}$ of a unital Poisson Banach algebra ${\mathcal{A}}$ to a unital Poisson Banach algebra ${\mathcal{B}}$ is a Poisson algebra homomorphism when h(xy) = h(x)h(y) holds for all $x,y{\in}\;{\mathcal{A}}$, and that every almost linear almost multiplicative mapping $h:{\mathcal{A}}{\rightarrow}{\mathcal{B}}$ is a Poisson algebra homomorphism when h(qx) = qh(x) for all $x\;{\in}\;{\mathcal{A}}$. Here the number q is in the functional equation given in the almost linear almost multiplicative mapping. We prove that every almost Poisson bracket $B:{\mathcal{A}}\;{\times}\;{\mathcal{A}}\;{\rightarrow}\;{\mathcal{A}}$ on a Banach algebra ${\mathcal{A}}$ is a Poisson bracket when B(qx, z) = B(x, qz) = qB(x, z) for all $x,z{\in}\;{\mathcal{A}}$. Here the number q is in the functional equation given in the almost Poisson bracket.

• Journal of the Korea Furniture Society
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• v.14 no.1
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• pp.47-58
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• 2003

Italian furniture companies play a leading role in the world furniture market and are acknowledged as good design trend leader. Their craftsmanship which has been handed down from the past is thoroughly applied to the process of design and production. Two well-known furniture companies in Italy, 'B&B Italia' and 'Kartell', are chosen as a case study model to grasp the key of their success. In general and objective material collecting and researching, this study aims at understanding the method of the advanced corporations' design development, marketing strategy and brand identity This study is expected to use as a research material for Korean furniture industry to escape from the serious depression after IMF shock.

• Journal of Life Science
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• v.16 no.5
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• pp.828-833
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• 2006

Maize (Zea mays L.) pistil cell death and stamen cell arrest are pivotal process on the sex determination, which diverges from bisexual state of floral meristem to unisexual state in staminate or pistillate floret. We investigated the temporal and spatial distribution of cell cycle gene expression during maize sex determination. The positive regulatory genes of cell cycle, cyclin A, cyclin B, cyclin dependent kinase (CDK) and Mad2 were highly expressed in the developing pistil and stamen but the expression was disappeared in the dying pistil and arresting stamens. In contrast, the negative regulatory genes of cell cycle, Wee1 and CDK inhibitor (CKI) were expressed in the arresting stamens in the wild-type ear and tasselseed2 mutant tassel, however, these genes were not detected in dying pistil although the cyclin B gene expression was disappeared. These results suggest that both the pistil cell death and stamen cell arrest process in maize sex determination are involved in cell cycle regulation, but the different expression patterns of negative regulatory cell cycle genes in the arresting stamens and aborting pistils suggest that the two processes may have distinctive modes of action.