• Microbiology and Biotechnology Letters
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• v.49 no.1
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• pp.65-74
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• 2021

Serine proteases are the most versatile proteolytic enzymes with tremendous applications in various industrial processes. This study was designed to investigate the biochemical properties, critical residues, and the catalytic potential of alkaline serine protease using in-silico approaches. The primary sequence was analyzed using ProtParam, SignalP, and Phyre2 tools to investigate biochemical properties, signal peptide, and secondary structure, respectively. The three-dimensional structure of the enzyme was modeled using the MODELLER program present in Discovery Studio followed by Molecular Dynamics simulation using GROMACS 5.0.7 package with CHARMM36m force field. The proteolytic potential was measured by performing docking with casein- and keratin-enriched residues, while the effect of the inhibitor was studied using phenylmethylsulfonyl fluoride, (PMSF) applying GOLDv5.2.2. Molecular weight, instability index, aliphatic index, and isoelectric point for serine protease were 39.53 kDa, 27.79, 82.20 and 8.91, respectively. The best model was selected based on the lowest MOLPDF score (1382.82) and DOPE score (-29984.07). The analysis using ProSA-web revealed a Z-score of -9.7, whereas 88.86% of the residues occupied the most favored region in the Ramachandran plot. Ser327, Asp138, Asn261, and Thr326 were found as critical residues involved in ligand binding and execution of biocatalysis. Our findings suggest that bioengineering of these critical residues may enhance the catalytic potential of this enzyme.

• Journal of the Korean Society of School Health
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• v.33 no.3
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• pp.139-147
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• 2020

Purpose: The study aimed to identify factors affecting unintentional injuries at school focusing on violent experiences in adolescents. Methods: The study used the raw data of the Korea Youth Risk Behavior Web-based Survey (2019). A total of 60,040 students were included in the analysis. Descriptive statistics, χ2 test, and multiple logistic regression were performed, using SPSS version 25.0. General characteristics, gender, school, academic achievement, living with family, socioeconomic status, vigorous physical activity, perceived stress, sleep satisfaction, high caffeine drinking, risky alcohol drinking, smoking, sexual experience, drug use, and safety education, were adjusted for and the relationship between unintentional injuries and violence was analyzed. Results: The results showed 2.4% of the adolescents had experienced violence and 24.7% had suffered unintentional injuries. Violent experiences had a significant effect on unintentional injuries among adolescents. Injuries were 1.93 times higher among adolescents who had experienced violence. Gender, school levels, high-intensity physical activity, stress, sleep satisfaction, high caffeine drinks, dangerous drinking, drug use, and safety education were associated with unintentional injuries among adolescents. Conclusion: Adolescents at high risk of suffering violence and injuries should be identified and assessed preemptively. In addition, it is necessary to implement systematic school health-centered safety education programs in order to prevent violence and injuries.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.9 no.2
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• pp.129-135
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• 2015

The objective grading system for the facial palsy is needed. In this study, the facial palsy grading system was developed with combination of three dimensional image processing and Nottingham scale. The developed system is composed of 4 parts; measurement part, image processing part, computational part, facial palsy evaluation & display part. Two web cam were used to get images. The 8 marker on face were recognized at image processing part. The absolute three dimensional positions of markers were calculated at computational part. Finally, Nottingham scale was calculated and displayed at facial palsy evaluation & display part. The effects of measurement method and position of subject on Nottingham scale were tested. The markers were measured with 2-dimension and 3-dimension. The subject was look at the camera with $0^{\circ}$ and $11^{\circ}$ rotation. The change of Scale was large in the case of $11^{\circ}$ rotation with 2-dimension measurement. So, the developed system with 3-dimension measurement is robust to the orientation change of subject. The developed system showed the robustness of grading error originated from subject posture.

• 대한공업교육학회지
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• v.31 no.2
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• pp.233-256
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• 2006

The purpose of this study was to analyse between foreign curriculum and our high school industrial technology subject-matter curriculum, to review trend and stream of curriculum revision, and purpose and content system of subject-matter. This study was conducted through reviewing literature; research reference, journal, book, and Web materials. in this study, comparative target country was restricted to Japan, U. S. A., U. K., and N. Z., Australia that administer to similar subject with our industrial technology subject-matter. The major finding of this study were as follows: 1. A similar subject-matter with our industrial technology subject0matter was Japan' 'foundation of industrial technology' and 'project research', U. S. A.' 'technology' and etc, U. K.' 'design and technology', and N. Z.' 'technology', 'New South Wales in Australia' design and technology'. 2. The result of analysis to purpose and strength of subject-matter, our' industrial technology subject-matter was oriented to knowledge, understanding and career search in industrial area. but, the other was emphasized technological problem solving by process-based method with thinking and action. 3. In the curriculum content, our country was treat to content area of a broad industrial world. on the other hand, Japan; relationship between human and technology, environment, process technology and product technology, project research. U. S. A.; technology content standards by knowledge, process and context, U. K., N. Z., and Australia were focused 'design process'. Based on above results, the recommendation can be established as follows: 1. A study on the implementation of industrial technology curriculum. 2. A study on the perception and need assessment of expert and stakeholder about purpose and content system. of industrial technology subject-matter.

• The Journal of Society for e-Business Studies
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• v.21 no.2
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• pp.151-161
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• 2016

In the era of the Web 2.0, characterized by the openness, sharing and participation, it is easy for internet users to produce and share the data. The amount of the unstructured data which occupies most of the digital world's data has increased exponentially. One of the kinds of the unstructured data called personal online product reviews is necessary for both the company that produces those products and the potential customers who are interested in those products. In order to extract useful information from lots of scattered review data, the process of collecting data, storing, preprocessing, analyzing, and drawing a conclusion is needed. Therefore we introduce the text-mining methodology for applying the natural language process technology to the text format data like product review in order to carry out extracting structured data by using R programming. Also, we introduce the data-mining to derive the purpose-specific customized information from the structured review information drawn by the text-mining.

• Journal of the Korean Society for Library and Information Science
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• v.44 no.2
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• pp.29-50
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• 2010

The purpose of this study presents a model of the integrated online library service with LMS (Learning Management System). The new library service is called 'Library on Blackboard' which was developed and implemented by Murray State University Libraries in Murray, Kentucky, USA. This user-centered service with applied information technology has the potential to enhance the quality of the library performance in university libraries. The introduction of the Learning Management System in South Korea has a potential to enhance the education environment and promote new library services. With the emergence of new information technologies and growing popularity in online classes and distance education programs, the importance and usage of Learning Management Systems in higher education has increased.

• Ocean and Polar Research
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• v.29 no.4
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• pp.401-410
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• 2007

In Korea the study of marine heterotrophic protists started in the late 1980s, and since the early 1990s many studies have been conducted in various marine environments. In this article, studies on the distribution and abundance of protists and the biotic interactions(bacteria-protists, phytoplankton-protists) conducted in Korean coastal waters are reviewed, and a field study is reported and discussed. The field study in Masan Bay was carried out from February 2004 to November 2005 at seven selected stations representative of the bay. During the study, the mean abundance of heterotrophic bacteria and the mean concentration of chlorophyll-a were $2.1{\times}10^6\;cells\;mL^{-1}$ and $9.8{\mu}g\;L^{-1}$, respectively. Heterotrophic protists consisted of heterotrophic dinoflagellates, heterotrophic nanoflagellates(excluding dinoflagellates) and ciliates, and their abundances were means of $7.9{\times}10^4\;cells\;L^{-1}$, $1.2[\times}10^3\;cells\;mL^{-1}$, and $4.0{\times}10^4\;cells\;L^{-1}$, respectively. Generally, the chlorophyll-a concentra+CZ14tions and the abundances of heterotrophic bacteria and protists were higher in the inner zone of the bay, where there are high concentrations of organic matters, than in the middle and outer zones. Using the grazing rates of heterotrophic nanoflagellates on bacteria previously reported in this area, it can be calculated that about 69% of bacterial producton was removed by HNF grazing activity. About 24% of initial chlorophyll-a concentration was removed by microzooplankton grazing activity. In conclusion, this study suggests that in Masan Bay heterotrophic protists control the growth of bacteria and phytoplankton, and heterotrophic protists represent an important link of bacterial & microalgal biomass to higher trophic levels.

• Journal of the Korea Institute of Building Construction
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• v.22 no.1
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• pp.11-21
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• 2022

Currently, in the domestic construction industry, the free web method has been emerging as a potential solution to the shortage of skilled workers due to the prolonged COVID-19 crisis, as it helps in securing economic feasibility through shortening the construction period and reducing labor costs. To consider one part of the construction method, in this study, the bending behavior according to the load was evaluated for the SY slope-type beam formwork, which was manufactured at a factory, assembled with rebar, brought into the site, and then poured into the site. For the SY Beam standard cross-sectional shape, a cross-sectional dimensional width of 400mm and depth 600mm determined through structural modeling using the MIDAS GEN program were applied. A total of 6 specimens were made with a member length of 5,000mm, 5 specimens and one RC specimen in the comparison group were manufactured in real-size format using the thickness of the steel plate(0.8, 1.0, 1.2mm) as a variable, and bending experiments were performed. In the bending test, the steel plate deck showed high initial stiffness and maximum strength as it yielded, which showed that it sufficiently contributed to the flexural strength. It is judged that additional analysis and experimental studies for 1.05, 1.1, and 1.15mm are needed to derive the appropriate steel plate thickness and the method for calculating the tensile force contribution of the steel plate to secure the manufacturing, construction and economic feasibility of SY Beam in the future.

• Journal of the Korean Recycled Construction Resources Institute
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• v.5 no.3
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• pp.282-289
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• 2017

The flexural behavior tests of UHPC segmental Box girder which has 160MPa compressive strength and 15.4m length were carried out. The test variables are area of prestressing wires, volume fraction of steel fibers and longitudinal reinforcing bars in upper flange and web. PS tendons which has 32 strands of 15.2mm diameter in lower flange, 24 strands and 14 strands in lower flange were arranged and volume fraction of 2%, 1.5% and 1.0% is used in box girder concrete. UHPFRC box girder which has 32 strands in lower flange showed the over reinforcement and brittle behavior. UHPFRC box girder which has 24 strands showed the similar peak load as 32 strands girder and ductile behavior as large deflection. UHPFRC box girder which has 14 strands showed half of the peak load of 24 strands box girder and ductile behavior. After the application of the formular for the reinforcement index to the behavior of the UHPFRC box girders, reinforcement index does not determine the characteristic of behavior of UHPFRC box girder exactly. So the index should consider the dimension precisely and modify the reference value corresponding to the 0.005 strain of the prestressing strands.

• Journal of Information Technology Services
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• v.16 no.1
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• pp.99-118
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• 2017

Growing importance of online security on certification, many of the web-related security technology introduced day by day. Especially, using smart certificate (USIM) is recognized as one of the most safe and convenient method for the security of public certificate. The purpose of this study is to find the factors that affect the Intention of switching to smart certificate for the general public who use public certificate service. Based on migration theory, this study determined the relationship between pull factors, push factors and mooring factors which can affect the intention of switching to smart certificate. To empirically analyze the proposed hypothesis, the statistical analysis were conducted based on the response from 350 public certificate service users using SPSS 22.0 and Smart PLS 2.0. The results of this study can be summarized as follows. First, system quality, one of the pull factors has positive influence on perceived ease of use. On the other hand, the other pull factor, service quality has positive influence on both perceived ease of use and perceived usefulness. Second, push factors, user experience and information security awareness have positive influence on perceived ease of use only. Third, mooring factor, switching cost does not have influence on switching intention while perceived usefulness has positive influence on. The results of this research can be used for whom has interest to the switching intention of public certificate service for online security and provide the proper understanding about smart certificate service mechanism.