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검색결과 2,274건 처리시간 0.032초

Solubilization of Proteins from Human Lymph Node Tissue and Two-Dimensional Gel Storage

  • De Marqui, Alessandra Bernadete Trovo;Vidotto, Alessandra;Polachini, Giovana Mussi;De Mattos Bellato, Claudia;Cabral, Hamilton;Leopoldino, Andreia Machado;De Gois Filho, Jose Francisco;Fukuyama, Erica Erina;Settanni, Flavio Aurelio Parente;Cury, Patricia Maluf;Bonilla-Rodriguez, Gustavo Orlando;Palma, Mario Sergio;Tajara, Eloiza Helena
    • BMB Reports
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    • 제39권2호
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    • pp.216-222
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    • 2006
  • In the present study, we compared six different solubilization buffers and optimized two-dimensional electrophoresis (2-DE) conditions for human lymph node proteins. In addition, we developed a simple protocol for 2-D gel storage. Efficient solubilization was obtained with lysis buffers containing (a) 8M urea, 4% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate), 40 mM Tris base, 65 mM DTT(dithiothreitol) and 0.2% carrier ampholytes; (b) 5M urea, 2M thiourea, 2% CHAPS, 2% SB 3-10 (N-decyl-N, N-dimethyl-3-ammonio-1-propanesulfonate), 40mM Tris base, 65 mM DTT and 0.2% carrier ampholytes or (c) 7M urea, 2M thiourea, 4% CHAPS, 65 mM DTT and 0.2% carrier ampholytes. The optimal protocol for isoelectric focusing (IEF) was accumulated voltage of 16,500 Vh and 0.6% DTT in the rehydration solution. In the experiments conducted for the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), best results were obtained with a doubled concentration (50 mM Tris, 384 mM glycine, 0.2% SDS) of the SDS electrophoresis buffer in the cathodic reservoir as compared to the concentration in the anodic reservoir (25 mM Tris, 192 mM glycine, 0.1% SDS). Among the five protocols tested for gel storing, success was attained when the gels were stored in plastic bags with 50% glycerol. This is the first report describing the successful solubilization and 2D-electrophoresis of proteins from human lymph node tissue and a 2-D gel storage protocol for easy gel handling before mass spectrometry (MS) analysis.

The Relationship between Microbial Characteristics and Glomalin Concentrations of Controlled Horticultural Soils in Gyeongnam Province

  • Kim, Min Keun;Ok, Yong Sik;Heo, Jae-Young;Choi, Si-Lim;Lee, Sang-Dae;Shin, Hyun-Yul;Kim, Je-Hong;Kim, Hye Ran;Lee, Young Han
    • 한국토양비료학회지
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    • 제47권2호
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    • pp.107-112
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    • 2014
  • Glomalin has been suggested as an enhancer for soil stability by promoting the aggregation. In this study, we examined the concentrations of glomalin and microbial characteristics in 25 controlled horticultural soils sampled from Gyeongnam Province. Total glomalin had a significant positive correlation with soil organic matter (p < 0.01), soil microbial biomass carbon (p < 0.05), and dehydrogenase activity (p < 0.05) in controlled horticultural soils. In addition, the total glomalin had a significant positive correlation with concentrations of total fatty acid methyl esters, Gram-negative and Gram-positive bacteria, fungi, and arbuscular mycorrhizal fungi in controlled horticultural soils (p < 0.001). In conclusion, the concentration of total glomalin could be an indicator of microbial biomass richness for sustainable agriculture in controlled horticultural soils.

인천광역시 도서지역에서 번식하는 저어새(Platalea minor)의 육추 교대에 관한 비교 연구 (A Comparative Study of Nest Attendance Patterns of Chick-rearing Black-faced Spoonbills (Platalea minor) in Incheon, South Korea)

  • 박종현;이기섭;권인기;정훈
    • Ocean and Polar Research
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    • 제42권1호
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    • pp.89-95
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    • 2020
  • Our study was conducted to examine the nest attendance of the Black-faced Spoonbill (Platalea minor) from 2015 to 2018 at two Islets located in Incheon, South Korea. We visited study sites in March-April and set up the remote sensor cameras at two breeding sites (Mae-do, Guji-do) to observe chick-rearing behavior. Mean nest bout length at Mae-do (female: 4.2 ± 0.1 hr, male: 4.0 ± 0.1 hr) was shorter than at Guji-do (female: 5.3 ± 0.2, male: 6.0 ± 0.3 hr), and trip duration at Mae-do (female: 4.4 ± 0.1 hr, male: 4.0 ± 0.1) was also shorter than at Guji-do (female: 7.0 ± 0.2 hr, male: 7.0 ± 0.3). Mean nest bout length and trip duration at both study sites decreased as chick rearing progressed. Males attended their nests during the daytime, and females attended their nests during the nighttime. Our results showed that females and males' duties were divided by the diel cycle, but the pattern of nest attendance could change depending on the environment of their breeding sites during the chick-rearing period.

잎브로콜리에서 배추좀나방 요방제 수준 설정 (Economic Threshold for the Diamondback Moth (Plutella xylostella L., Lepidoptera: Plutellidae) on Leaf Broccoli (Brassica oleracea var. botrytis italiana))

  • 최선우;임주락;김주희;이공준;문영훈;정성수;이기권
    • 한국응용곤충학회지
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    • 제54권1호
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    • pp.1-6
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    • 2015
  • 잎브로콜리에 심각한 피해를 주는 배추좀나방의 적절한 방제시기 설정을 위한 기초자료로 이용하기 위하여 유충접종밀도와 잎브로콜리 피해량의 관계를 분석하여 경제적피해허용수준과 요방제 수준을 설정한 결과는 다음과 같다. 배추좀나방 3~4령 유충을 주당 0, 0.5, 1.0, 1.5, 2.0마리를 5월 24일에 접종하고, 6월 1일, 6월 9일, 6월 18일에 발생밀도와 엽수량을 조사한 결과, 초기 접종밀도가 증가할수록 배추좀나방의 발생 밀도는 증가하였고 시간이 경과할수록 엽수량은 감소하였다. 이를 토대로 배추좀나방의 접종밀도와 수량감소율을 회귀식을 이용하여 분석한 결과 y=1636-394x ($R^2=0.79^{***}$)로 추정되었다. 잎브로콜리에서 5% 피해율을 경제적 피해한도로 볼 때 배추좀나방의 경제적피해허용수준은 10주당 2~3마리이고, 요방제 수준은 10주당 1-2마리 수준으로 추정된다.

식쌍성 DO Cas의 광도곡선과 시선속도곡선의 분석 (AN ANALYSIS OF THE LIGHT AND RADIAL VELOCITY CURVES OF DO CAS)

  • 안영숙;김호일;이우백
    • Journal of Astronomy and Space Sciences
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    • 제17권2호
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    • pp.181-188
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    • 2000
  • DO Cas는 최근 Shaw (1990)에 의해 근접촉쌍성으로 분류된 공전주기 0.68일의 식쌍성이다. 짧은 공전주기를 가짐에도 불구하고 Mannino (1958) 이후 분광관측에 의한 시선속도 곡선이 발표되지 않고 있다. 그런데 Hill (1991)은 Mannino의 시선속도곡선이 오류를 포함하고 있는 것으 로 보인다고 하였다. 이 연구에서 우리는 Asiago 천문대에 의뢰하여 Mannino가 분석한 분광관측 사진건판을 입수하여 PDS로 측정하여 IRAP로 재분석하였다. 이 시선속도곡선과 1998년에 관측한 BVR 광도곡선을 Wilson-Devinney 방법으로 동시에 분석하였다. 그 결과 DO Cas가 접촉 또는 주성이 로쉬엽을 채우고 있는 접촉에 매우 가까운 준분리형 쌍성임을 확인하였으며 또한 이 계의 절대량을 구하였다.

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Yonsei Cancer Center QA Set을 이용한 6DoF Couch의 이동 정확성 검증 (Verify Image-Guided Shifts for 6DoF Couch using Yonsei Cancer Center QA Set)

  • 정동민;박효국;윤종원;이상규;김주호;조정희
    • 대한방사선치료학회지
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    • 제29권1호
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    • pp.7-18
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    • 2017
  • 목 적: 영상유도를 통한 6DoF Couch의 이동 정확성의 검증을 위해 QA Set을 제작하였고, 그 유용성을 평가하였다. 대상 및 방법: 6DoF Couch와 CBCT가 설치된 두 대의 선형가속기를 대상으로 하였으며, 자체 제작한 YCC QA Set을 이용하여 수평수직(Translation; TX, TY, TZ)과 회전(Rotation, Pitch; RX, Roll; RY, Yaw; RZ) 여섯방향의 Off-Set 값이 설정된 Penta-Guide Phantom의 CBCT영상을 각각 15회에 걸쳐 획득하였다. 이를 통해 기준영상(Reference Image)과 보정영상(Registration Image)을 비교하였으며, 보정된 6DoF Couch의 이동정확성을 실측하여 오차를 분석하였다. 결 과: 기준영상과 보정영상의 Air Cavity에 해당하는 Pixel들은 모두 30에서 66 사이에 포함되어 보정정확도가 높게 나타났다. 6DoF Couch의 Off-set의 보정 결과 값과 실측치의 비교에서 수평수직방향의 오차는 TX방향은 $0.25{\pm}.18mm$ TY방향은 $0.25{\pm}.25mm$ TZ방향에서 $0.36{\pm}.2mm$로 나타났다. 그리고 회전방향의 오차는 RX방향은 $0.18{\pm}.08^{\circ}$ RY방향은 $0.26{\pm}.09^{\circ}$ RZ방향에서 $0.11{\pm}.08^{\circ}$로서, 임의의 값에 대하여 정확하게 보정되었다. 결 론: YCC QA Set을 이용해 매우 간단한 방법으로 6DoF Couch의 수평수직방향뿐 아니라 회전방향의 오차에 대한 검증을 할 수 있었으며, 이는 6DoF Couch의 Daily IGRT QA를 수행함에 있어서 유용할 것이라 사료된다.

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고추 세균점무늬병 발생에 따른 수량 변화와 경제적 방제수준 설정 (Determination of Economic Control Thresholds for Bacterial Spot on Red Pepper Caused by Xanthomonas campestris pv. vesicatoria)

  • 김주희;정성수;이기권;임주락;이왕휴
    • 식물병연구
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    • 제21권2호
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    • pp.89-93
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    • 2015
  • 고추 세균점무늬병이 수량에 미치는 영향을 조사하여 경제적 방제수준을 설정하고자 시험을 수행하였다. 노지고추 세균점무늬병 발생정도와 수량과의 회귀식은 Y=-0.724X+281.58, $R^2=0.78$, $r=-0.88^{**}$ 성립하였다. 노지고추 세균점무늬병 발생정도와 수량감소와의 회귀식은 Y=0.813X+15.95, $R^2=0.78$, $r=0.88^{**}$ 성립하였다. 성립된 회귀식을 이용하여 노지 고추 수량에 영향을 주지 않는 수준의 세균점무늬병 방제적기는 발생 발병엽률이 30.3% 이하이었다. 또한 고추 세균점무늬병 경제적 방제수준은 노지 고추 세균점무늬병 발생정도가 16.3% 이하로 결정되었다.

곡류 및 그 가공품 중 아플라톡신과 데옥시니발레놀의 안전성 평가 (The Safety Assessment of Aflatoxins and Deoxynivalenol in Cereals and Their Products)

  • 김영수;김영숙;김명길;이성봉;이주예;오상헌;정유정;서미영;성진희;이완;이정복;윤미혜
    • 한국식품위생안전성학회지
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    • 제28권2호
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    • pp.158-167
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    • 2013
  • This study was carried out to assess the safety of cereals and their products (20 species, 205 samples) distributed in Gyeonggi province by analyzing contamination levels such as exposure frequency of Aflatoxins and Deoxynivalenol (DON). Aflatoxins were detected in 16 (8%) samples in total with a range of 0.01~27.88 ${\mu}g/kg$, including 3 of 9 bake flour (33%), 2 of 6 dried corn (33%), 3 of 12 frying flour (25%) and 2 of 11 sorghum (18%). DON was found in 56 (27%) samples including 11 of 13 adlay (85%), 6 of 8 wheat (75%), 6 of 10 processed corn for popcorn (60%), 6 of 11 sorghum (55%) and 7 of 16 barley (44%) with a range of 2.2~754.4 ${\mu}g/kg$. In particular, both Aflatoxins and DON were detected in 8 samples (2 millet, 2 wheat, 1 sorghum, 1 adlay, 1 dried corn, 1 bake flour) simultaneously. As a result of this study, we found that an adult is exposed to Aflatoxins of $0.80({\times})10^{-3}{\mu}g/kg$ b.w./day and DON of 0.18 ${\mu}g/kg$ b.w./day. The quantity of exposure to DON amounted to 18.5 percentage level compared with PMTDI 1 ${\mu}g/kg$ b.w./day suggested on JECFA, therefore we can assess that the possibility of health risks by intake cereals distributed in Gyeonggi province is low. However the concentration of Aflatoxins in one dried corn was 27.88 ${\mu}g/kg$ which was over the maximum residue limits (MRL) suggested on internal and external level, the monitoring about mycotoxin should be conducted on continuously.

A likely exoplanet around F5 supergiant ${\alpha}$ Persei near the Cepheid instability strip

  • 이병철;한인우;박명구;김강민
    • 천문학회보
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    • 제36권1호
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    • pp.28.2-28.2
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    • 2011
  • To search for and study the nature of the long-periodic variations of massive stars, we have been carrying out a precise radial velocity (RV) survey for supergiants. Here, we present high-resolution RV measurements of ${\alpha}$ Per which lies near the Cepheid instability strip from November 2005 to February 2011 using the fiber-fed Bohyunsan Observatory Echelle Spectrograph (BOES) at Bohyunsan Optical Astronomy Observatory (BOAO). The orbital solution yields a period of 129 days, a 2K amplitude of 80 m/s, and an eccentricity of 0.1. Assuming a possible stellar mass of 7.3 $M{\bigodot}$, we estimate the minimum mass for the planetary companion to be 7.5 MJup with the orbital semi-major axis of 0.97 AU. We do not find the correlation between RV variations and chromospheric activity indicator (Ca II H & K region). The Hipparcos photometry and bisector velocity span (BVS) do not show any obvious correlations with RV variations. These analyses suggest that ${\alpha}$ Per is a pulsating supergiant that hosts an exoplanet. If the 129 days variations of ${\alpha}$ Per do not come from an exoplanet but Cepheid-like pulsations, the theoretical boundary of the Cepheid instability strip may need to be extended to the bluer side.

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