• Plant Breeding and Biotechnology
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• 제6권4호
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• pp.337-353
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• 2018

We used Illumina/HiSeq sequencing for analysis of gene expression profiling among four maize seed types (dent, CM3 and CM6; waxy, CM5 and CM19) at 10 DAP (days after pollination). A total of 88,993,000 (CM3), 103,817,340 (CM6), 103,139,640 (CM5), and 66,978,958 (CM19) sequence reads were generated with read lengths of about 0.9, 1.0, 1.0, and 0.7 billion bp, respectively. We obtained 69.1 (CM3), 71.0 (CM6), 71.2 (CM5), and 71.8% (CM19) high quality reads from the raw data and compared them with reference RNA sequences in a public DB (NCBI). It was revealed that mapped reads were 58%, 63%, 62%, and 62% of the EST reference in CM3, CM6, CM5 and CM19, respectively; and more than 51,000 genes were expressed based on RPKM criteria (over 0.25 value) in each CM3, CM6, CM5, and CM19 inbred line. In differentially expressed gene (DEG) analysis, we found that 3,527 genes were differentially expressed by at least two-fold with 1,709 upregulated in the two waxy inbred lines and 1,818 upregulated in the two dent inbred lines. We also detected genes for the sucrose and starch biosynthesis pathways based on BINs, and different expression patterns between waxy and dent inbred lines were shown for the gene set for starch synthesis, such as sh2, bt2, du1, wx1, and ae1. Although some genes were more expressed in dent lines, most genes for starch synthesis were much expressed in waxy lines. Especially, there was greater expression of the sus2 gene in both waxy lines compared with the dent lines.

• 한국작물학회지
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• 제31권1호
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• pp.16-18
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• 1986

보리 F$_2$용 종자에 대한 찰성유전자(wx)와 분장성 전분유전자(fra)에 대한 유전현상을 구명하고 또 이들 두 유전자간에 관계를 구명키 위하여 실험을 한바 그 결과는 아래와 같다. 1 찰성 및 전분상성 배분인자는 각각 1개의 열성유전자가 관여하였으며, 2. 찰성과 분장성 전분유전자는 각각 독립적으로 작용하였다.

• 한국작물학회지
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• 제46권2호
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• pp.69-77
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• 2001

'Chalok 1/Cooktail 51' corns, supersweet corn gene controlled by either brittle-l (bt l) or shrunken-2(sh2) gene introduced into waxy corn, showed agronomic characteristics between supersweet corn and waxy corn. The ears were harvested at different development stages from 15 to 35 days after silking (DAS). Ear diameter of Cocktail 51 and 'Cocktail 51'/Chalok 1 increased from 15 DAS to 30 DAS and little increased thereafter, but that of Chalok 1/Cocktail 51 and 'Chalok l' increased until 35 DAS. Diameter of ear extension increased more in Cocktail 51 and Chalok 1/Cocktail 51 corn than Chalok 1. Ear fresh weight of Cocktail 51 decreased later 30 DAS but those of the other hybrids were vice versa. Rate of super-sweet kernels per ear of Chalok 1/Cocktail 51 corns was about 38 %. Development, and elongation of kernel were much more prominent in supersweet kernel than in waxy kernel, but fresh weight increased higher in waxy kernel than supersweet kernel. Moisture content in kernel decreased from 15 DAS to 35 DAS. Total sugar content of the kernel increased until 25 DAS, and that of Cocktail 51 kernel showed the highest among of them. After cooked by steam, flavor and mastication feeling rate cooked by steam of Cocktail 51 and Chalok 1/Cocktail 51 were increased from 15 DAS to 25 DAS and markedly decreased thereafter. But those of Chalok l/Cocktail 51 and, Chalok 1 were decreased after 30 DAS. These results suggested that the optimum harvest date for fresh supersweet corn (Cocktail 51), Cocktail 51/Chalok 1 seems to be about 20 DAS and Chalok 1/Cocktail 51 and waxy com (Chalok 1) was about 25 DAS.

• 한국작물학회지
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• 제38권1호
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• pp.46-54
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• 1993

최근 육성된 내한성이며 찰쌀보리인 품종들이 확대 보급을 통하여 재배지대의 북상과 수량에 대한 안정성을 제고시키기 위하여 찰과, 찰피, 메과 및 메피성의 isogenic line을 이용한 찰ㆍ메 및 피ㆍ과성인자가 발아 및 출현률에 미치는 영향을 검사한 결과는 다음과 같다. 1.수확 전일 강우시는 피성에 비하여 과성의 발아율이 4-6% 낮았으며, 5일 인공강수처리에서는 과성의 발아율로 현저히 저하되었다. 2. 탈곡기회전속도가 600rpm에서 1000rpm으로 상승할 때 피성의 발아율이 100%에서 90%로 저하되었으나, 과성은 98%에서 76%로 크게 저하되었다. 3. 과성에서 발아율보다 출현률이 피성에 비하여 큰 차이가 있었으며 파종심도가 깊을수록 그 차이가 컸다. 4. 파종심도, 고탈곡회전수가 수확시의 강우순으로 피성보다 과성의 출현율은 크게 저하되었다. 5. 탈곡시 찰성배유유부자에 따른 쌀보리의 배손상정도와는 관련이 없었으며, 6. 탈곡시 배의 손상은 겉보리는 영에 의한 보호로 적었으나 영의 보호가 없는 쌀보리는 컸다. 7. 탈곡시 탈곡시의 회전수를 600, 900rpm으로 탈곡만 하거나 또는 탈곡후 탈망회전속도를 750rpm으로 탈망한 종자의 손상입률 및 발아율은 겉보리는 큰 영향이 없었으며, 쌀보리는 매우 높아 채종시 주의가 필요하다. 8. 수발아율은 찰성인자 도입시에는 메성에 비해 높았으나 과성인자 도입시에는 차이가 없었다. 9. 과맥의 출현률 향상을 위하여는 수확후 강우피해를 방지하고 탈곡시 회전속도를 낮추며 파종심도를 적절히 조절하여 찰성인자 도입시에는 등숙과 정중 휴안성이 낮아 수발아률이 높은 점을 고려한 육종적 조치를 하여야 찰과성 품종의 육성보급시 재배면적의 북상 및 확대를 기하고 수량의 안정성을 제고할 수 있다.

• 한국임상수의학회지
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• 제41권2호
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• pp.88-94
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• 2024

Pet rabbits are affected by the highly contagious ectoparasite Psoroptes (P.) ovis, which carries significant economic implications for the global rabbit industry. Accurate identification of the mite species remains essential to implement effective treatment and control strategies. Two approximately one-year-old female pet rabbits were admitted to the Veterinary Teaching Hospital of Chungbuk National University due to excessive scratching of the ears and the presence of waxy debris within the ear canals. Mites were isolated from the waxy debris extracted from the ear canals and subsequently identified as Psoroptes spp. through microscopic examination. Species confirmation was achieved through mitochondrial cytochrome c oxidase subunit 1 (cox1) gene analysis. The analysis revealed the mites to be P. ovis based on cox1 gene sequences. The deposited GenBank accession numbers for these sequences are OR985022 and OR985023. This represents the first report of mitochondrial cox1 gene sequences of P. ovis isolated from pet rabbits in South Korea.

• 식물분류학회지
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• 제38권4호
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• pp.371-388
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• 2008

Coryloideae 35집단에 대한 계통 유연관계를 알아보기 위하여 핵 DNA의 waxy 유전자와 엽록체 DNA의 atpB-rbcL intergenic spacer 지역에 대한 염기서열을 분석하였다. Waxy 유전자 분석에서 Coryloideae의 4개 속은 단계통군을 형성하였으며, 개암나무속은 단계통군을 형성하면서 군내군의 가장 기부에 분계조(clade)를 형성하였다. Ostryopsis속은 서어나무속과 새우나무속을 위한 자매군을 형성하였으며, 새우나무속 역시 단계통군을 형성하였다(BS=86, PP=99). AtpB-rbcL 분석에서는 Ostryopsis속이 아과 내에서 가장 기부에 분계조를 형성하였다. 개암나무속은 서어나무속-새우나무속과 Ostryopsis속의 중간에 위치하였고(BS=98, PP=100), 서어나무속은 새우나무속 분류군들과 함께 높은 지지도(BS=100, PP=100)를 가지고 하나의 clade를 형성하였다. Waxy 유전자 분석에서 서어나무속의 Carpinus절은 단계통을 형성하였지만 Distegocarpus절은 병계원군(paraphyletic group)으로 나타났다. 개암나무속의 경우는 2개의 subclade를 형성하였지만 속내 절 또는 아절 등의 분류계급과는 일치하지 않았다. AtpB-rbcL 분석에서는 대부분의 분류군들이 각각의 clade내에서 polytomy를 형성하여 차이를 보였다. 이상의 결과에서 두 유전자의 계통분석 결과가 뚜렷하게 불일치하였고, 특히 Ostryopsis속의 위치와 새우나무속의 단계통 여부, 그리고 서어나무속과 새우나무속의 유연관계가 가장 큰 차이를 보였다. 따라서 이들에 대한 추가적인 연구가 필요한 것으로 판단된다.

• 한국육종학회지
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• 제40권3호
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• pp.250-257
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• 2008

Knowledge of genetic diversity and of the genetic relationships among elite breeding materials has had a significant impact on the improvement of crops. In maize, this information is particularly useful in i) planning crosses for hybrid and line development, ii) in assigning lines to heterotic groups and iii) in plant variety protection. We have used the SSR technique to study the genetic diversity and genetic relationships among 76 Korean waxy corn accessions, representing a diverse collection from throughout Korea. Assessment of genetic diversity among members of this group was conducted using 30 microsatellite markers. Among these 30 microsatellite markers, we identified a total of 127 alleles (with an average of 4.2 and a range of between 2 and 9 alleles per locus). Gene diversity at these 30 microsatellite loci varied from 0.125 to 0.795 with an average of 0.507. The cluster tree generated with the described microsatellite markers recognized two major groups with 36.5% genetic similarity. Group I includes 63 inbred lines, with similarity coefficients of between 0.365 and 0.99. Group II includes 13 inbred lines, with similarity coefficients of between 0.45 and 0.85. The present study indicates that the 30 microsatellite loci chosen for this analysis are effective molecular markers for the assessment of genetic diversity and genetic relationships between Korean waxy corn accessions. Specifically, this study's assessment of genetic diversity and relationships between a set of 76 Korean waxy corn inbred lines will be helpful for such activities as planning crosses for hybrid and line development and association mapping analyses of maize breeding programs in Korea.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 Grop Science and Biotechnology in the 21st Century
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• pp.30.1-30.1
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• 2006

• 농업과학연구
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• 제34권2호
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• pp.131-134
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• 2007

1. 본 시험에 공시된 7개의 찰옥수수 계통중에서 간장은 IK/H26-2, Heunchal, 239-3, IK/H26-1 계통이 대조품종인 Chalok 1호보다 큰 반면에 Local-1, Local-2, IK/LE 계통은 작게 나타났다. 2. 착수고의 경우 Local-2, IK/LE는 각각 23.3cm, 35cm로 대조품종보다 낮았으며, 주당 분얼수는 대조품종인 찰옥1호와 약간의 차이를 보였다. 3. 이삭의 경직경은 IK/H26-1, IK/H26-2, Local-2 239-3 계통 등에서 대조품종보다 낮았고 Local-1, IK/LE, Heunchal 계통 등에서는 다소 굵었다. 4. 출사일수는 Local-1, Local-2, IK/LE 등에서 대조품종보다 2일정도 빨랐고 239-3, Heunchal등에서는 각각 61일, 60일로 다소 늦은 반면에 IK/H26-1, IK/H26-2 등에서는 대조품종과 동일하였다. 특히, 공시 수집종 계통 중에서 Local-2가 55일로 가장 짧은 기간을 나타내어 조숙 찰옥수수 교잡종의 육성 자료로 이용에 유리할 것으로 판단되었다.

• 한국작물학회지
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• 제43권2호
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• pp.89-94
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• 1998

Granule bound starch synthase (GBSS), also known as the '"waxy protein'", is responsible for the synthesis of amylose in the amyloplasts of cereal crops. In hexaploid wheat (Triticum aestivum L.), GBSS is involved in amylose synthesis and rolls as an important factor to determine flour quality and end-use quality in food products. Genes on three Wx loci have been found to encode GBSS in common wheats. We developed techniques for the purification and separation of GBSS in wheat. Three major GBSS isoforms, which were encoded by the genes on three loci, Wx-A1, Wx-B1, and Wx-D1 migrating differently by one dimensional SDS-po-lyacrylamide gel electrophoresis (1D SDS-PAGE), were identified. GBSS from 66 Korean hard and soft winter wheats were purified and determined for their Wx loci and four of them were identified possessing a null allele either at the Wx-A1 and Wx-B1 loci. With help of identification of three GBSS isoforms using 1D SDS-PAGE system, we are able to identify and monitor Wx gene expressions in breeding materials for developing waxy or partial waxy wheats without experiencing consecutive selecting generations.cting generations.