• Title/Summary/Keyword: Watermelon mosaic virus

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Incidence of Watermelon Mosaic Virus in Cucurbits (박과 작물에 발생하는 Watermelon Mosaic Virus에 관한 연구)

  • Lee Soon Hyung;Lee Key Woon
    • Korean journal of applied entomology
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    • v.20 no.4 s.49
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    • pp.191-195
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    • 1981
  • Cucurbits including pumpkin (Cucurbita pepo), gourd (Lagenariaa siceraria), cucumber (Cucumis sativus), melon(Cucumis melo) and watermelon(Cucurbita anguria) were diseased with mosaic symptoms. The causal virus was identified as watermelon mosaic virus(WMV). The WMV was transmitted by Myzus persicae Sulzer, and no seed borne virus was found. The virus caused large local lesions on the inoculated leaves of the Chenopodium amaranticolor and mosaic symptom on the upper leaves of Cucumis melo, Cucumis sativus, Lagenaria siceraria, Cucurbita anguria and Cucurbita pepo. There were no symptoms on the inoculated leaves of the Nicotiana tabacum var. Bright yellow, Nicotiana glutinosa, Vigna unguiculata. Petunia hybrida and Datura stramonium. Thermal inactivation point was $55\~65^{\circ}C$, dilution end point was $10^{-4}\;10^{-5}$ and longevity in vitro of the virus was $7\~8$ days. The virus showed positive reaction against watermelon mosaic virus antiserum in microprecipitin tests. The virus particles were flexuous rods in size of 750 nm.

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Virus Diseases Occurred on Watermelon in Jeonnam Province (전남지역의 수박에 발생하는 바이러스 병 발생 실태)

  • Ko, Sug-Ju;Lee, Yong-Hwan;Cha, Kwang-Hong;Park, Jin-Woo;Lee, Su-Heon;Yang, Kwang-Yeol
    • Research in Plant Disease
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    • v.10 no.1
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    • pp.39-43
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    • 2004
  • Recent occurrence of virus diseases on watermelon plants cultivated in Jeonnam province was investigated from 1998 to 2002. While virus diseases were severely occurred on watermelon cultivated in green house in 1998, those of open field were severer than in green house since 2000. When 128 samples collected from different fields were examined by electron microscopy, 87.8% of the samples contained rod-shaped or filamentous virus particles. RT-PCR analysis of the samples revealed that Cucumber green mottle mosaic virus (CGMMV) was only detected from collected samples at May. Watermelon mosaic virus (WMV) was most frequently found and CGMMV and Zucchini yellow mosaic virus (ZYMV) were slightly at June and July. However Cucumber mosaic virus (CMV) and Papaya ringspot virus (PRSV) have not been detected.

Ultrastructural Aspects of the Mixed Infections of Watermelon Mosaic Potyvirus and Cucumber Green Mottle Mosaic Tobamovirus Isolated from Watermelon

  • Kim, Jeong-Soo;Cho, Jeom-Deog;Park, Hong-Soo;Kim, Kyung-Soo
    • The Plant Pathology Journal
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    • v.16 no.4
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    • pp.211-215
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    • 2000
  • Symptoms on 4 varieties of watermelons inoculated with watermelon mosaic potyvirus II isolated from watermelon (WMV-W) were severe mosaic and leaf malformation while those inoculated with cucumber green mottle mosaic tobamovirus from watermelon (CGMMV-W) were mild mosaic and chlorotic spots. Inoculation of the mixture of WMV-W and CGMMV-W produced extremely severe mosaic along with necrotic spots and general necrosis. Doubly infected plants were also stunted. Cells infected with WMV-W or CGMMV-W alone exhibited the intrinsically ultra-structural properties of each virus infection. WMV-W induced potyvirus-characteristic cylindrical inclusions in the cytosol. Virus particles were orderly aligned along the tonoplasts. CGMMV-W induced tobamovirus-characteristic stacked crystalline arrays of virus particles in the cytosol. Cells infected doubly with WMV-W and CGMMV-W contained striking cytopathic effects that were not present in single infection of each virus. The unique ring structure, nonagon, was that a single potyvirus particle was surrounded by 9 CGMMV-W tobamovirus particles.

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Incidence of Virus Diseases in Major Cultivated Areas of Watermelon and Melon in Chungbuk Province (충북지역 주산지 수박, 멜론에서의 바이러스 발생현황)

  • Jong-Woo Han;Young-Uk Park;Cheol-Ku Youn;Seok-Ho Lee;Taek-Goo Jeong;Hong-Soo Choi;Mi-Kyeong Kim
    • Research in Plant Disease
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    • v.29 no.1
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    • pp.88-93
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    • 2023
  • To investigate the incidence status of viruses in major cultivated areas of watermelon and melon in Chungbuk Province, samples were collected from 2020 to 2021 in vinyl greenhouse of Jincheon and Eumseong and examined for virus infection using reverse transcription polymerase chain reaction. Of the six viruses on watermelon that was analyzed in this study, watermelons were infected with cucumber mosaic virus (CMV), watermelon mosaic virus (WMV), cucumber green mottle mosaic virus (CGMMV), and cucurbit aphid-borne yellows virus (CABYV). The incidence rate of CMV was 20.9-35.0%, WMV 0.4-15.8%, CGMMV 1.6-38.5%, and CABYV was 3.5-3.7% from 2020 to 2021. But strangely, there were no incidence of zucchini yellow mosaic virus and cucurbit chlorotic yellows virus (CCYV) during investigation. From this result, we knew the major virus was CGMMV on watermelon in Chungbuk Province. Molecular diagnosis assays of the two melon viruses, showed that melons were infected with CABYV and CCYV from 2020 to 2021. The incidence rate of CABYV was 53.9-92.2% and CCYV was 2.7-20.8%. The incidence of CABYV was high in melon cultivation of Jincheon and Eumseong, Chungbuk. Afterwards, it is necessary to establish a control management strategy for reduce the incidence of CABYV. Furthermore, we must pay attention that of CCYV even if the incidence was low.

Isolation and Characterization of Watermelon Isolate of Cucumber green mottle mosaic virus(CGMMV-HY1) from Watermelon Plants with Severe Mottle Mosaic Symptoms

  • Shim, Chang-Ki;Han, Ki-Soo;Lee, Jung-Han;Bae, Dong-Won;Kim, Dong-Kil;Kim, Hee-Kyu
    • The Plant Pathology Journal
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    • v.21 no.2
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    • pp.167-171
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    • 2005
  • We isolated the Cucumber green mottle mosaic virus(CGMMV) particles from watermelon leaves and designated as CGMMV-HY1 as a watermelon isolate and attempted to characterize the pathogenic isolate responsible for such an epidemic in watermelon and also to monitor dominant viral isolates in greenhouse. The watermelon plants infected with CGMMV generally showed mottle mosaic, mosaic, growth stunting, necrosis and deformed fruit. The reactions of indicator plants to CGMMV-HY1 were the local lesions on Nicotiana tabacum cv. White Burley, Nicotiana tabacum cv. Samsun, and Chenopodium amaranticola, and the mosaic symptoms only on Cucumis sativus, but the CGMMV-HY1 did not infect Nicotiana sylvesytis, Datura stramonium, Chenopodium quinoa, and Petunia hybrida. Purified virus particles were rod-shaped and about 300 nm long. The coat protein (CP) of purified CGMMV-HY1 was single band with molecular weight of about 16.5 kDa which was confirmed by western blot analysis probed with monoclonal antibody of CGMMV-HY1. The genomic and subgenomic RNAs of 6.4 kb and 0.75 kb were revealed by the electrophoresis on 1.2% formaldehydedenatured agarose gel. Viral and complementary CGMMV-specific primer sets were designed for spanning the genome using previously reported CGMMV sequences. A 464bp of CP gene of CGMMV-HY1 was amplified by RT-PCR and cloned into PGEM-T easy vector. The nucleotide sequence of CP gene of CGMMV-HY1 shared 98%, 99%, and 100% identities with that of CGMMV strains W, KOM, and KW respectively. Based on these results, we identified CGMMV-HY1 as a CGMMV isolate of watermelon, a member of Tobamovirus.

Construction of Antibodies for Detection and Diagnosis of Cucumber green mottle mosaic virus from Watermelon Plants

  • Shim, Chang-Ki;Lee, Jung-Han;Hong, Sun-Min;Han, Ki-Soo;Kim, Hee-Kyu
    • The Plant Pathology Journal
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    • v.22 no.1
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    • pp.21-27
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    • 2006
  • We immunized BALB/c mice with purified Cucumber green mottle mosaic virus isolate HY1 (CGMMV-HY1). Through the selection of positive clones that were grown on the HAT medium, four sensitive monoclonal clones (CG99-01, CG99-02, CG99-03, and CG99-04) were selected from 500 Hypoxanthine-guanine phosphoribosyltransferase positive hybridoma cells. Four sensitive clones of CGMMV-HYI were determined as IgM type of the subclass of mouse immunoglobulins Ig group. The titer of monoclonal antiserum against CGMMVHY1 was estimated 1:12,800 by the indirect ELISA. Although monoclonal antibodies (MAbs) from CG99-01 and from CG99-04 cross-reacted with Zucchini green mottle mosaic virus and Kyuri green mottle mosaic virus, MAb from the cell line CG99-03 was highly specific to CGMMV. No MAbs cross-reacted with Cucumber mosaic virus-Fny. Only CG99-04 reacted with Pepper mild mottle virus weakly and CG99-02 reacted with both CGMMV and KGMMV. CGMMV was detected from the rind of watermelon fruit by DAS-ELISA of CGMMV-HY1, but not from the flesh of watermelon. Average seed transmission rate of CGMMV in watermelon was $24\%$ from symptomatic watermelon collected from 5 regions of Gyeongnam province. CGMMV was detected by DAS-ELISA with specific MAb of CGMMVHY1 periodically from root stock, during the sequential process for nursery seedling in Haman. Necrotic spots on cotyledons of root stock seedling progressed to reveal the typical symptomatology on the primary leaves of scion upon grafting. Here, we have established MAb based ELISA system, which could accurately detect CGMMV from watermelon seeds, nursery seedlings, transplants and field samples from greenhouse or open out door field as well.

Ultrastructural Aspects of the Mixed Infections of Watermelon Mosaic Potyvirus Isolated from Pumpkin and Cucumber Green Mottle Mosaic Tobamovirus from Watermelon

  • Cho, Jeom-Deog;Kim, Jeong-Soo;Park, Hong-Soo;La, Yong-Jun;Kim, Kyung-Soo
    • The Plant Pathology Journal
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    • v.16 no.4
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    • pp.216-221
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    • 2000
  • Mixed infection of watermelon mosaic potyvirus II isolated from pumpkin (WMV-P) and cucumber green mottle mosaic tobamovirus from watermelon (CGMMV-W) caused extremely severe symptoms such as progressive silting and death of watermelon plants. Single infections of either WMV-P or CGMMV-W on the same hosts produced only vein clearing and/or mosaic on the upper leaves. In cells infected with WMV-P, potyvirus-characteristic inclusions of pinwheels, scrolls and cylindrical inclusions were present in the cytosol. Parallel arrays of virus particles in the tonoplast were also common. In cells infected with CGMMV-W, virus particles occurred as stacked-bands of scattered randomly in the cytosol and vacuoles in all type cells. Many cells also contained vesiculated mitochondria with fibril-containing vesicles. Cells infected mixedly with WMV-P and CGMMV-W contained structural features that were not observed in cells infected singly with the two viruses. A particle of WMV-P potyvirus was surrounded by evenly spaced nine particles of CGMMV-W tobamovirus, which made a unique nonagon ring. The angled layers having $60^{\circ}$-$135^{\circ}$ were alternating layer, herringbone, crosshatching and ladder figures.

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Soil Transmission of Cucumber green mottle mosaic virus and Its Control Mensures in Watermelon (수박에 오이녹반모자이크바이러스의 토양전염과 예방대책)

  • Choi, Gug-Seoun;Kim, Jae-Hyun;Kim, Jeong-Soo
    • Research in Plant Disease
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    • v.10 no.1
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    • pp.44-47
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    • 2004
  • Soil transmission ratio of Cucumber green mottle mosaic virus (CGMMV) was 0.2 to 3.5 % in watermelon growing fields naturally infested with the virus. Biological activities of CGMMV lost after 17 months in moist well-aerated soil but still continued more than 33 months in waterlogged soil. To inhibit the virus infection through soil, the roots of watermelon seedlings were soaked in 10% solution of skim milk prior to transplanting. The seedlings treated with skim milk solution were not infected, while 5.0 to 7.6% out of control seedlings were infected. The roots treated with skim milk were coated with membrane around the roots under scanning electron microscope.

Novel Pathogenic Strain of Watermelon mosaic virus Occurred on Insam (Panax ginseng) (인삼(Panax ginseng)에 발생한 Watermelon mosaic virus의 새로운 병원성 계통)

  • Jung, Won-Kwon;Nam, Moon;Lee, Joo Hee;Park, Chung Youl;Kim, Byoung Hoon;Park, Eun Hye;Lee, Min-A;Kim, Mi-Kyeong;Choi, Hong-Soo;Lee, Jun Seong;Kim, Jeong-Soo;Choi, Jin Kook;Kwon, Tae Ryong;Lee, Key-Woon;Lee, Su-Heon
    • Research in Plant Disease
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    • v.19 no.4
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    • pp.331-337
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    • 2013
  • A disease, supposedly caused by a virus, was observed from Insam (Panax ginseng) fields of Punggi in year 2006. It has long believed to be a physiological disorder. However, the incidence of the disease has increased every year. When several samples were observed under electron microscope, filamentous virus-like particles were observed. The nucleotide sequences of the virus were analyzed by RT-PCR with specific primer sets derived from the results of DNA chip. The results indicated that the disease was caused by Watermelon mosaic virus (WMV). It revealed that the result of the biological assay by the virus was different from that of WMV previously found in other crops. Therefore, this is the first report that WMV causes the disease in P. ginseng and the virus is named to be WMV-Insam.

Multiplex Reverse Transcription Polymerase Chain Reaction Assay for Simultaneous Detection of Five Cucurbit-infecting Viruses.

  • Lee, Su-Heon;Kim, Sang-Mok;Kim, Woo-Chang;Lee, Key-Woon
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.150.1-150
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    • 2003
  • A single-step multiplex reverse transcription polymerase chain reaction (RT-PCR) assay was developed for the simultaneous detection of five cucurbit-infecting viruses: cucumber mosaic virus (CMV), watermelon mosaic virus 2 (WMV2), zucchini yellow mosaic virus (ZYMV), cucumber green mottle mosaic virus (CGMMV), and kyuri green mottle mosaic virus (KGMMV). The multiplex RT-PCR provides a simple and rapid method for detecting various viruses in cucurbit plants, which will help diagnose many cucurbit plants at a time.

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