• Title/Summary/Keyword: WaterML

Search Result 2,364, Processing Time 0.028 seconds

Cholinomimetic Properties of a Water-Soluble Fraction from Mulberry Leaves in Rats

  • Lee, Ju-Seon;Chung, Sung-Hyun;Lee, Yong-Sup;Jin, Chang-Bae
    • Biomolecules & Therapeutics
    • /
    • v.13 no.1
    • /
    • pp.26-31
    • /
    • 2005
  • The present study examined effects of a water-soluble fraction from mulberry leaves (ML water fraction) on the circulatory and autonomic nervous systems, which were compared with those of acetylcholine (ACh) used as a reference drug in order to elucidate its mechanism of action. Intravenous administration of ACh or a ML water fraction produced temporary depressor and tachycardiac responses in a dose-dependent manner in unrestrained, conscious Sprague-Dawley rats. The systemic hemodynamic effects of ACh and a ML water fraction were almost completely blocked by pretreatment with atropine, a muscarinic antagonist. The depressor responses to ACh and a ML water fraction were slightly enhanced and prolonged by pretreatment with neostigmine, an anticholinesterase, whereas the tachycardiac responses were remarkably blocked by pretreatment with pentolinium, a ganglionic blocking agent. In vitro experiments using the ileum isolated from rats showed that ACh and a ML water fraction increased ileal contractility in a dose-dependent manner. The increases in ileal contractility were also completely abolished in the presence of atropine. Finally, the specific binding of [$^3H$]quinuclidinyl benzilate, a muscarinic antagonist, to rat cortical synaptic membranes was inhibited by a ML water fraction in a concentration-dependent manner with an IC$_{50}$ value of 9.5 mg/ml. The results suggest that the effects of a ML water fraction are mediated through direct stimulation of muscarinic cholinergic receptors by unknown cholinomimetic substance(s) contained in that fraction.

Microbiological Pollution Investigation of Lowland Spring Water at the Jeju City Waterfront, Jeju Island (제주도 내 제주시 해안가 저지대용천수의 미생물학적 오염도 조사)

  • Kim, Man-Chul;Han, Yong-Jae;Heo, Moon-Soo
    • Journal of Life Science
    • /
    • v.22 no.3
    • /
    • pp.324-331
    • /
    • 2012
  • Bacteriological examination of spring water in Jeju City was conducted. A total of 21 spring water samples were collected from January to April, 2010. During the study period, the range of temperature was 0.6 to $15.4^{\circ}C$, and the results of the analyses showed that hydrogen ion concentrations (pH) for spring water were 0.43 to 7.9. Salinity levels for the samples averaged from 3.0 to 1.64%, and levels of water-dissolved oxygen were 1.85 to 6.06 mg/l. The range of total coliforms in spring water samples at 21 stations located in the designated spring water were <1.8->1,600 MPN/100 ml. Furthermore, the range of geometric means of total coliforms was 9.9-151.6 MPN/100 ml, while the range of fecal coliforms in spring water samples at 21 stations located in the designated spring water area was <1.8->1,600 MPN/100 ml. Finally, the range of geometric mean of fecal coliforms was 3.1-151.6 MPN/100 ml. The level of microbial contamination was examined in 21 samples for indications of bacterial contamination such as heterotrophic bacteria, Escherichia coli O157:H7, Salmonella spp., Vibrio parahaemolyticus, Staphylococcus aureus, and Shigella spp. were frequently detected in the spring water. Salmonella spp., Shigella spp., Vibrio parahaemolyticus, and S. aureus were detected in the range of $0-0.5{\times}10^1$, $0-0.1{\times}10^1$, $0-0.1{\times}10^1$, and $0-0.3{\times}10^1$ CFU/ml, respectively, while E. coli O157:H7 was not detected in the examined spring water samples.

Difference in Volume Perception according to Cup Shape in Normal Weight and Overweight Female College Students (정상체중과 과체중 여대생을 대상으로 컵의 형태에 따른 인지량의 차이에 관한 연구)

  • Lee, Myung-Ok;Chang, Un-Jae
    • Journal of the Korean Dietetic Association
    • /
    • v.20 no.1
    • /
    • pp.63-69
    • /
    • 2014
  • The volume perception of water according to cup shape was examined in female college students divided into a normal weight group (<23 body mass index, n=51) and an overweight group (${\geq}$23 body mass index, n=48). The perceived water volume was recorded by participants after they observed the water volume in cups with different shape (highball and tumbler) and with different base height (high base and low base), which contained the same amount of water (150 ml). The normal weight group perceived a water volume of 238.5 ml (highball) and 201.6 ml (tumbler) for the high base cups. The overweight group perceived a volume of 245.6 ml (highball) and 188.8 ml (tumbler). For the low base cups, the normal weight group perceived a water volume of 207.8 ml (highball) and 104.1 ml (tumbler). The overweight group perceived a volume of 202.2 ml (highball) and 100.4 ml (tumbler). Both the normal weight and overweight groups perceived significantly more liquid in the highball cups compared to the tumbler cups. In addition, both groups perceived significantly more liquid in the high base cups compared to the low base cups. However, there were no significant differences in the estimated variance of perceived volume between the normal weight and overweight groups according to the cup shape and cup base height. In conclusion, cup shape and base height potentially affects volume perception. Therefore, modifying cup shape may be used in a weight control program to help control caloric beverage consumption.

Improvement of proliferation efficiency of strawberry 'Maehyang' treated by coconut water in tissue culture ('매향' 딸기의 조직배양 시 coconut water 처리에 따른 기내 증식효율 향상)

  • Kim, Hye Jin;Choi, Mi Ja;Lee, Jong Nam;Suh, Jong Taek;Kim, Ki Deog;Kim, Yul Ho;Hong, Su Young;Kim, Su Jeong;Sohn, Hwang Bae
    • Journal of Plant Biotechnology
    • /
    • v.47 no.3
    • /
    • pp.242-247
    • /
    • 2020
  • This experiment was performed to determine the concentration of coconut water that is most effective in promoting the growth of 'Maehyang' strawberry in tissue culture. Cultivars in this experiment consisted of 'Maehyang' grown in the presence of 0, 20, 40, 60, 80, 100 ml·L-1 of coconut water which was added to a medium mixed with BA 0.5 mg·L-1 and IBA 0.1 mg·L-1. Morphological variation tests and SSR detection with coconut water were conducted to determine variations in proliferation. The proliferation rate of 'Maehyang' strawberry improved with the coconut water treatment compared to non-treatment. In particular, the proliferation rate with 100 ml·L-1 coconut water treatment increased by about 4 times. When 'Maeyang' was treated with 100 ml·L-1 of coconut water, two morphological variants occurred without genetic variation. Therefore, the results suggest that 100 ml·L-1 of coconut water can be used to mass-produce "Maeyang" strawberry without causing genetic variations.

Effects of Sam so on Pye Tang Water Extract on the plasma cortisol Concentration and Plasma $CO_2$ in the rabbit (삼소온폐탕(蔘蘇溫肺湯) 전탕액(煎湯液)이 가토(家兎) 혈장(血漿) Cortisol 농도(濃度) 및 혈장(血漿) $CO_2$에 미치는 영향(影響))

  • Sin, Jo-Yeong;Han, Sang-Whan;Lee, Byung-Soon
    • The Journal of Internal Korean Medicine
    • /
    • v.11 no.2
    • /
    • pp.112-119
    • /
    • 1990
  • To evaluate effects of Sam so on Pye Tang(SSOPT) Water Extract plasma cortisol concentration and plasma $CO_2$ in the Rabbit. The results obtained were as follows. 1. Intravenous administration of SSOPT water extract at the dose of 0.5ml/kg remarkably increased plasma cortisol concentration on 1 hour. 2. Intravenous adminitration of SSOPT Water extract at the does of 1.0ml/kg significantly decreased plasma $CO_2$ on 1 hour. 3. Intravenous adminitration of SSOPT Water extract at the does of 0.5ml/kg remarkable decreased plasma $Na^+$ from 1 to 4 hour and 1.0ml/kg significantly decreased plasma $Na^+$ on 1 hour. 4. Intravenous administration of SSOPT Water extract at the does of 1.0ml/kg remarkably increased plasma $k^+$ from 1 to 3 hour. 5. Intravenous administration of SSOPT water extract at the does of 0.5ml/kg significantly decreased plasma $CI^-$ on 3, 4 hours. and 1.0ml/kg remarkably increased plasma $CI^-$ on 4 hour. These results suggest that therapeutic action of SSOPT water extract for athma may be reated with the increment of plasma cortisol concentration and the decrease plsma $CO_2.$.

  • PDF

Cytotoxicity of Crude Extracts of Rheum uudulatum L. with Human Kidney Epithelial Cell A498 (신장 상피세포주 A498을 이용한 대황(Rheum undulatum L.)추출물의 세포독성)

  • 나명석;진종언;조남철
    • The Korean Journal of Food And Nutrition
    • /
    • v.13 no.5
    • /
    • pp.460-464
    • /
    • 2000
  • We have evaluated cytotoxic effects of four crude extracts of methylene chloride, ethyl acetate, butanol, water layer isolated Rheum undulatum in A498 cell line, human kidney epithelial cells. The cytotoxic evalutation was measured by colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide(MTT) , neutral red(NR) and sulforhodamine protein B(SRB). These results obtained are as follows : MTT, NR and SRB quantities were significantly decreased in cultured A498 cells treated four crude extracts by increased concentrations. The cell cytotoxic effect of crude extracts of butanol layer was more stronger than others layer. The values of MTT$\sub$50/, NR$\sub$50/, SRB$\sub$50/ of crude extract of butanol layer and were measured both 0.63 mg/ml, 0.65 mg/ml, and 0.68 mg/ml, respectively and the values of water layer were 0.84 mg/ml, 0.82 mg/ml. and 0.80 mg/ml. respectively in cultured A498 cell line.

  • PDF

Bacterial Sanitation of Farm Water in Kyunggi Province (경기지역 목장수의 위생세균학적 분포)

  • 박석기;김성원;이강문;최성민;오영희
    • Journal of Environmental Health Sciences
    • /
    • v.19 no.2
    • /
    • pp.10-15
    • /
    • 1993
  • Hygienic condition of farm waters has an effect on the hygiene of dairy foods. So we examined Standard Plate Count, coliform, E. coli, heat-resistant bacteria, psychrophilic bacteria, Pseudomonas aeruginosa and enterococcus for the bacterial sanitation of 78 farm waters in Kyunggi Province. Of the 78 farm waters, the average number of psychrophilic bacteria was 750 $\pm$ 170/ml, SPC 440 $\pm$ 130/ml, Pseudomonas aeruginosa 130 $\pm$ 97/100/ml, Coliform 22 $\pm$ 17/ml, E. coli 10 $\pm$ 6/100/ml, Heatresistant bacteria 5 $\pm$ 1ml, and Enterococcus 2 $\pm$ 1/100/ml. The percent of over than 1000/ml in SPC and psychrophilic bacteria of 78 farm water was 11.5% and 23.1%, respectively. The rate of over than 10/ml in coliform and heat-resistant bacteria was 12.8% and 15.4%, respectively and the rate of over than 10/100 ml in E. coli, Pseudomonas aeruginosa and enterococcus was 8.9%, 33.3%, and 2.6%, respectively. Pseudomonas aeruginosa was no significant between any other indicator organisms, and psychrophilic bacteria was significant with only SPC but other indicator organisms were highly significant with each other.

  • PDF

In Vitro Antiviral Activity of Aqueous Extracts from Korean Medicinal Plants Against Influenza Virus Type A

  • Park, Kap-Joo;Lee, Hyung-Hoan
    • Journal of Microbiology and Biotechnology
    • /
    • v.15 no.5
    • /
    • pp.924-929
    • /
    • 2005
  • Boiled-water extracts from 101 Korean medicinal plants were tested in vitro for their inhibitory activity against influenza virus type A by means of a modified hemagglutination inhibition test. Thirteen of the 101 extracts exhibited strong anti-influenza virus type A activity at concentrations of less than $780\;{\mu}g/ml$. Out of the above 13 extracts, MW-40 (Chaenomeles speciosa), MW-88 (Citrus junos), and MW-100 (Zingiber officinale) exhibited marked antiviral activity in the concentration range of $0.195\;{\mu}g/ml$ to 100 mg/ml, $0.0487\;{\mu}g/ml$ to 100 mg/ml, and $0.0487\;{\mu}g/ml$ to 100 mg/ml, respectively. The extracts MW-88 and MW-100 were not cytotoxic to red blood cells, whereas MW-40 showed very weak cytotoxicity in the concentration range of 50 mg/ml to 100 mg/ml. Therefore, the present results demonstrate that boiled water extracts of 2 Korean medicinal plants, MW-88 and MW-100, have strong anti-influenza virus type A activity and no cytotoxic effects, and they may inhibit attachment of the virus to the cell and may be used for prophylaxis.

Cytotoxic Effect of Korean Traditional Prescriptions on the Human Gastric Cancer Cell Lines (한약처방제의 인체 위암 세포주에 대한 세포독성 효과에 관한 연구)

  • Kim, Eun-Hae;Eun, Young-Ah;Kang, Bong-Joo;Sung, Hyun-Jae;Park, Kap-Joo
    • Korean Journal of Pharmacognosy
    • /
    • v.28 no.4
    • /
    • pp.233-238
    • /
    • 1997
  • ln order to search for antigastric cancer agents from Korean traditional prescriptions. We selected 41 traditional prescriptions, based on a review of the Korean traditional medicine books. Both boiling water and methanol extracts were tested, by means of the Sulforhodamine B (SRB) protein assay. Six of the 41 water extracts; #3, #34, #35, #38, #40, #41 showed efficacy against gastric cancer cell (AGS: Human gastric carcinoma, ATCC HTB 103). #3 inhibited 50% cancer cell growth1 at the concentration of $152\;{\mu}g/ml$, #34, #35, #38, #40 and #41 inhibited 50% cancer cell growth at the concentration of $145\;{\mu}g/ml$, $129\;{\mu}g/ml$, $173\;{\mu}g/ml$, $10\;{\mu}g/ml$ and $19\;{\mu}g/ml$ respectively. Ten of the 41 methanol extracts; #1, #3, #32, #33, #35, #36, #37, #38, #41 were active. #1 inhibited 50% cancer cell growth at the concentration of $206\;{\mu}g/ml$, #3, #32, #33, #35, #36, #37, 738, #40, #41 inhibited 50% cancer cell growth at the concentration of $133\;{\mu}g/ml$, $159\;{\mu}g/ml$, $199\;{\mu}g/ml$, $147\;{\mu}g/ml$, $113\;{\mu}g/ml$, $187\;{\mu}g/ml$, $130\;{\mu}g/ml$, $9\;{\mu}g/ml$, $15\;{\mu}g/ml$ respectively. Prescription #3, #35, #38, #40, #41 were also interesting because both methanol and water extracts were active.

  • PDF

EFFECTS OF PAI MO SAN WATER EXTRACT ON THE PLASMA CORTISOL CONCENTRACTION AND ARTERIAL BLOOD $PCO_2,\;PO_2$ IN THE RABBIT (패모산(貝母散) 전탕액(煎湯液)이 가토(家兎) 혈장(血漿) Cortisol 농도(濃度) 및 동맥혈(動脈血) $PCO_2,\;PO_2$에 미치는 영향(影響))

  • Choi, Seon-Youb;Han, Sang-Whan
    • The Journal of Internal Korean Medicine
    • /
    • v.11 no.1
    • /
    • pp.127-140
    • /
    • 1990
  • To evaluate effects of Pai Mo San (PMS) water extract on the plasma cortisol concentration and arterial blood $PCO_2,\;PO_2$ in the rabbit. The results obtained were as follows: 1. Intravenous administration of PMS water extract at the dose of 0.2 ml/kg remarkably increased plasma cortisol concentration from 1 to 2 hours. 2. Intravenous administration of PMS water extract at the dose of 0.4 ml/kg plasma cortisol concentration showed more significant increase than the control group after 1,2,4 hours. 3. No change observed in the arterial blood $PO_2$ after intravenous administration of PMS water extract at the dose of 0.2 ml/kg used. 4. No change observed in the arterial blood $PO_2$ after intravenous administration of PMS water extract at the dose of 0.4 ml/kg used. 5. Intravenous administration of PMS water extract at the dose of 0.2 ml/kg arterial blood $PCO_2$ showed more significant decreased than the control group after 1,3 hours. 6. Intravenous administration of PMS water extract at the dose of 0.4 ml/kg significantly decreased arterial blood $PCO_2$. These results suggest that the therapeutic action of Pai Mo San water extract for Yeol Su (熱嗽), Wha Su (火熱), Gu Su (久熱) and anti-asthma maybe related with the increase of plasma cortisol, decrease of $PCO_2$.

  • PDF