Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Improvement of proliferation efficiency of strawberry 'Maehyang' treated by coconut water in tissue culture

'매향' 딸기의 조직배양 시 coconut water 처리에 따른 기내 증식효율 향상

  • Kim, Hye Jin (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Choi, Mi Ja (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Lee, Jong Nam (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Suh, Jong Taek (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Kim, Ki Deog (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Kim, Yul Ho (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Hong, Su Young (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Kim, Su Jeong (Highland Agricultural Research Institute, National Institute of Crop Science) ;
  • Sohn, Hwang Bae (Highland Agricultural Research Institute, National Institute of Crop Science)
  • 김혜진 (국립식량과학원 고령지농업연구소) ;
  • 최미자 (국립식량과학원 고령지농업연구소) ;
  • 이종남 (국립식량과학원 고령지농업연구소) ;
  • 서종택 (국립식량과학원 고령지농업연구소) ;
  • 김기덕 (국립식량과학원 고령지농업연구소) ;
  • 김율호 (국립식량과학원 고령지농업연구소) ;
  • 홍수영 (국립식량과학원 고령지농업연구소) ;
  • 김수정 (국립식량과학원 고령지농업연구소) ;
  • 손황배 (국립식량과학원 고령지농업연구소)
  • Received : 2020.04.29
  • Accepted : 2020.07.23
  • Published : 2020.09.30
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Abstract

This experiment was performed to determine the concentration of coconut water that is most effective in promoting the growth of 'Maehyang' strawberry in tissue culture. Cultivars in this experiment consisted of 'Maehyang' grown in the presence of 0, 20, 40, 60, 80, 100 ml·L-1 of coconut water which was added to a medium mixed with BA 0.5 mg·L-1 and IBA 0.1 mg·L-1. Morphological variation tests and SSR detection with coconut water were conducted to determine variations in proliferation. The proliferation rate of 'Maehyang' strawberry improved with the coconut water treatment compared to non-treatment. In particular, the proliferation rate with 100 ml·L-1 coconut water treatment increased by about 4 times. When 'Maeyang' was treated with 100 ml·L-1 of coconut water, two morphological variants occurred without genetic variation. Therefore, the results suggest that 100 ml·L-1 of coconut water can be used to mass-produce "Maeyang" strawberry without causing genetic variations.

본 실험은 딸기 조직배양 시 증식률 향상을 위해 coconut water의 적정처리 농도 구명하고자 실시하였다. 본 실험에 사용된 시험 품종은 '매향'이며, BA 0.5 mg·L-1와 IBA 0.1 mg·L-1을 혼합처리한 배지에 coconut water를 0, 20, 40, 60, 80, 100 ml·L-1로 첨가하여 실험하였다. Coconut water 처리 시 형태적 변이검정과 SSR 검정을 통해 변이 발생 유무를 확인하였다. '매향'은 무처리에 비해 coconut water 처리에서 증식률이 향상되었으며, 특히 coconut water 100 ml·L-1 처리에서 증식률이 약 4배 향상되었다. '매향'에 coconut water 100 ml·L-1 처리 시 형태적 변이체가 2개체 발생하였으나 유전적 차이는 보이지 않았다. 따라서 '매향' 조직배양 시 coconut water 100 ml·L-1를 처리하면 유전적 변이없이 대량증식이 가능할 것으로 판단된다.

Keywords

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