• The Korean Journal of Food And Nutrition
• /
• v.31 no.3
• /
• pp.328-334
• /
• 2018

The purpose of this study was to examine the effects of Maca water and/or ethanol extract on the nitric oxide (NO) production in human umbilical vein endothelial cells HUVAC and on erectile dysfunction in rats. Maca was extracted due to both solutions, which are water and ethanol. Each Maca extract was applied to HUVAC, and NO production was checked. Additionally, three different dosages (250, 500 and 1,000 mg/kg) of Maca ethanol extract was administered to Sprague-Dawley rats for 4 weeks. All rats were sacrificed and each sample was collected for analysis. The control rats received only the saline vehicle. The NO production of HUVAC was significantly increased by domestic and homemade Maca water extracted at $60^{\circ}C$ group. Both NO generation and testosterone release were not influenced due to the oral administration of Maca. In the EtOH group rats, the number of sperm was reduced compared to that of the control group. All Maca groups had a high number of sperm and each sperm count had increased as a result of the Maca extract dose. The results of this research suggest that Maca has a positive effect on male erectile dysfunction, which need to be examined further in future studies.

• The Korean Journal of Physiology and Pharmacology
• /
• v.8 no.1
• /
• pp.51-55
• /
• 2004

Sopungsungi-won has been used as a traditional medicine for diabetes and it has been proved to be a potential remedy for type 2 diabetes mellitus. We previously reported that water extract of Sopungsungi-won exhibits anti-diabetic effects both in vivo and in vitro experiments. In the present study, we have chosen to examined anti-apoptotic effect of Rheum undulatum, which is the main component of Sopungsungi-won, on pancreatic ${\beta}-cells$, HIT-T15, against hydrogen peroxide $(H_2O_2)$. oxidative stress. To investigate the anti-apoptotic effect of Rheum undulatum water extract (RUWE) against $H_2O_2-induced$ apoptosis in pancreatic ${\beta}-cell$ line of hamster, HIT-T15, MTT assay, DAPI staining, TUNEL assay, RT-PCR and caspase-3 enzyme assay were performed. The morphological analysis demonstrated that cells treated with $H_2O_2$ exhibited classical apoptotic features, while such changes was reduced in cells pre-treated with RUWE. In addition, RUWE pre-treated cells prior to $H_2O_2$ treatment induced increase of levels of bcl-2 expression and decrease of caspase-3 enzyme activity compared to cells treated with $H_2O_2$ only. These results provide the possibility of usage of RU in patients with progressively deteriorated diabetes.

• Herbal Formula Science
• /
• v.26 no.4
• /
• pp.307-318
• /
• 2018

Objectives : Endoplasmic reticulum (ER) stress designates cellular responses to the accumulation of misfolded and unfolded proteins in ER, which is related to a variety of liver diseases. Present study investigated the inhibitory effects of Litsea japonica flesh water extract (LJE) aganist ER stress. Methods : After HepG2 cells were pretreated with LJE and subsequently exposed to tunicamycin (Tm) or thapsigargin (Tg), expression of C/EBP homologous protein (CHOP), glucose regulated protein 78 kDa (GRP78), asparagine synthetase (ASNS), and endoplasmic reticulum DnaJ homologue 4 (ERDJ4) were determined by immunoblot and real-time PCR analysis. Three canonical signaling pathways in response to ER stress were examined to explore molecular mechanisms involved. Results : Pretreatment of 1 mg/mL LJE inhibited Tm- or Tg-induced CHOP expression, while L. japonica fruit water extract did not. In addition, LJE decreased the levels of GRP78, ASNS, and ERDJ4 mRNA by Tm. Moreover, phosphorylations of eukaryotic translation initiation factor $2{\alpha}$ and inositol-requiring enzyme 1, expression of nuclear form of activating transcription factor $6{\alpha}$, and transactivation of ER stress response element- and unfolded protein response element-harboring luciferase activities were inhibited by LJE pretreatment. Conclusions : Present results suggest that LJE would be a candidate to prevent or treat ER stress-mediated liver injuries.

• The Korea Journal of Herbology
• /
• v.28 no.5
• /
• pp.69-77
• /
• 2013

Objectives : The purpose of this study was to characterize the effect of the Ethanolic extract of Stachys sieboldii and Lycopus lucidus on the learning and memory impairments induced by scopolamine. Methods : The genetic difference of Stachys sieboldii and Lycopus lucidus were observed with RAPD analysis. The cognition-enhancing effect of Stachys sieboldii and Lycopus lucidus was investigated using a passive avoidance test, Y-maze test and the Morris water maze test in mice. Drug-induced amnesia was induced by treating animals with scopolamine (1 mg/kg, i.p.). Results : As a result of RAPD analysis, Stachys sieboldii and Lycopus lucidus Radix was found to be genetically different and The results of learning memory analysis showed that Stachys sieboldii extract-treated group (500 mg/kg, p.o.) and the tacrine-treated group (10 mg/kg, p.o.) significantly ameliorated scopolamine-induced amnesia based on the Passive avoidance Y-maze test and Water maze test. And these results are same manner in DPPH radical scavenger effect and Acetylcholineseterase inhibition effect. These results suggest that Stachys sieboldii extract maybe a useful cognitive impairment treatment, and its beneficial effects are depending on the origin plants. Conclusions : Commercially available Stachys sieboldii Radix consists of two original plant, one of them people misuse. To clarify the origin of the plant Memory tests were performed. These results suggest that 80% Ethanol extract of Stachys sieboldii showed significant anti-amnestic and cognitive-enhancing activities related to the memory processes, and these activities were parallel to treatment duration and dependent of the learning models.

• Journal of Korean Society of Water and Wastewater
• /
• v.33 no.2
• /
• pp.159-167
• /
• 2019

In this paper the potential of the principal component analysis(PCA) technique for the application of detecting leaks in water pipe networks was evaluated. For this purpose the PCA was conducted to evaluate the relevance of the calculated outliers of a PCA model utilizing the recorded pipe flows and the recorded pipe leak incidents of a case study water distribution system. The PCA technique was enhanced by applying the computational algorithms developed in this study which were designed to extract a partial set of flow data from the original 24 hour flow data so that the effective outlier detection rate was maximized. The relevance of the calculated outliers of a PCA model and the recorded pipe leak incidents was analyzed. The developed algorithm may be applied in determining further leak detection field work for water distribution blocks that have more than 70% of the effective outlier detection rate. However, the analysis suggested that further development on the algorithm is needed to enhance the applicability of the PCA in detecting leaks by considering series of leak reports happening in a relatively short period.

• Journal of Applied Biological Chemistry
• /
• v.58 no.4
• /
• pp.377-381
• /
• 2015

The content analysis of 6-gingerol, which is an active compound, in Zingiber spp. (Z. officinale and Z. mioga) and their commercial foods (ginger teas and powders) was conducted using high-performance liquid chromatography. A reverse phase system was used, with a gradient solvent system of water and acetonitrile. The 6-gingerol content was highest in the methanol extract of Z. officinale root (17.09 mg/g extract) and ginger powder B (15.92 mg/g extract). The results demonstrated that this method was simple and reliable for the quality control of Zingiber commercial foods.

• Biomedical Science Letters
• /
• v.28 no.1
• /
• pp.34-41
• /
• 2022

In this study, conditions for extraction of functional component from Nypa fruticans wurmb was optimized. The yield by extraction with 30% ethanol (LE30, 42.12%) was higher than those hot water extraction (LDW 33.32%), 50% ethanol (LE50, 40.12%) and 70% ethanol (LE70, 34.5%). The extract was purified and analyzed by GC MS. The prevailing compounds found in extract were Cyclodecasiloxane-, pentadecanoic acid, -eicosane, undecanal and tridecanoic acid. The presence of saturated and unsaturated fatty acids in ethanolic extract vindicate the use of this plant to treat many diseases in traditional medicine. The total phenolic contents in the LDW, LE30, LE50, LE70 extract were 128±1.65 mg/g, 205±2.3 mg/g, 210±4.23 mg/g and 180±5.6 mg/g, respectively. The DPPH was highest in LE70 extract (1,000 ㎍/mL, 81.14%), ABTS was highest in LE50 extract (1,000 ㎍/mL, 84.14%). The protective effects against oxidative stress in raw 264.7 cell imparted by the LE50 extract was better than those imparted by the other extracts. The findings of the present study suggest that 50% ethanol is best solvent for extraction of Nypa fruticans Wurmb, considering yield, polyphenol content, and antioxidant activities with extraction cost.

• YAKHAK HOEJI
• /
• v.57 no.5
• /
• pp.348-356
• /
• 2013

The present study investigated the effects of Hovenia Dulcis (HD) fruit extract powder on the improvement of physical activity, especially exercise capacity. Forty mice were divided into 4 groups including normal controls, negative controls, 100 (HD-100) and 200 (HD-200) mg/kg HD fruit extract powder groups for 5-times exercises using treadmill. Normal control did not performed treadmill running but others did 5-times for 10 days. HD fruit extract powders were administrated orally one-times per day for 10 days before treadmill exercise and normal and negative controls were fed with excipient water. After 5-times exercise, blood biochemical analysis showed that aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine phosphokinase (CK) activities and blood lactate concentration were statistically increased in negative controls than in normal controls. They were decreased in HD fruit extract powder groups, compared with negative controls. These results were considered as the effects of HD fruit extract powders on reduced tissue damages during exercise. Other measured indices did not reveal remarkable differences. All together, these results suggest that HD fruit extract powders may enhance the exercise performance by recovering the exercise-fatigue via blood lactate concentration by reducing blood LDH activity and via reduction of blood CK and AST activity.

• Korean Journal of Environmental Agriculture
• /
• v.13 no.3
• /
• pp.301-310
• /
• 1994

This experiment was conducted to test the fungicidal activity of extracts from 50 medicinal plants to powdery mildew (Sphaerotheca fulinginea) and identify the bioactive substances. Among the medicinal plants tested, the water extract of Rheum undulatum was the most effective in spore germination inhibition, which inhibited by 100% at 200-fold dilution. Also, 50-fold dilution of water extract, 100-fold dilution of alcohol extract, 500-fold dilution of crude extract from Rheum undulatum and even 1000-fold dilution of reference chemical inhibited powdery mildew of cucumber more than 60%. 500-fold dilution of crude extract inhibited powdery mildew of cucumber 100% by twice spray treatment. There was phytotoxcity at the 100-fold dilution, but was not recognized this injury at the 500-fold dilution of crude extract. From our research to identify bioactive substance using HPLC, GLC and Mass spectrum analysis, it indicated that Rheum undulatum extract contained tentatively 1,8-dihydroxy-3-methyl-9,10-anthracenedione and 1,8-dihydroxy-3-methoxy-6-methyl-9,10-anthracenedione.

• Korean Journal of Remote Sensing
• /
• v.39 no.4
• /
• pp.425-440
• /
• 2023

Monitoring water surface has become one of the most prominent areas of research in addressing environmental challenges.Accurate and automated detection of watersurface in remote sensing imagesis crucial for disaster prevention, urban planning, and water resource management, particularly for a country where water plays a vital role in human life. However, achieving precise detection poses challenges. Previous studies have explored different approaches,such as analyzing water indexes, like normalized difference water index (NDWI) derived from satellite imagery's visible or infrared bands and using k-means clustering analysis to identify land cover patterns and segment regions based on similar attributes. Nonetheless, challenges persist, notably distinguishing between waterspectralsignatures and cloud shadow or terrain shadow. In thisstudy, our objective is to enhance the precision of water surface detection by constructing a comprehensive water database (DB) using existing digital and land cover maps. This database serves as an initial assumption for automated water index analysis. We utilized 1:5,000 and 1:25,000 digital maps of Korea to extract water surface, specifically rivers, lakes, and reservoirs. Additionally, the 1:50,000 and 1:5,000 land cover maps of Korea aided in the extraction process. Our research demonstrates the effectiveness of utilizing a water DB product as our first approach for efficient water surface extraction from satellite images, complemented by our second and third approachesinvolving NDWI analysis and k-means analysis. The image segmentation and binary mask methods were employed for image analysis during the water extraction process. To evaluate the accuracy of our approach, we conducted two assessments using reference and ground truth data that we made during this research. Visual interpretation involved comparing our results with the global surface water (GSW) mask 60 m resolution, revealing significant improvements in quality and resolution. Additionally, accuracy assessment measures, including an overall accuracy of 90% and kappa values exceeding 0.8, further support the efficacy of our methodology. In conclusion, thisstudy'sresults demonstrate enhanced extraction quality and resolution. Through comprehensive assessment, our approach proves effective in achieving high accuracy in delineating watersurfaces from satellite images.