Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Process Optimization of Nypa fruticans Wurmb Extract using Mixed Solvent and its Functional Component Analysis

  • Kim, Jin (Dental Healthcare & Clinical Trial Center, Chosun University) ;
  • Kim, Han Sung (Him Co., Ltd, Business Incubation Center, Chosun University) ;
  • Son, Jang Wan (School of Infomatics & Product Design, Chosun University) ;
  • Moon, Seong Yong (Dental Healthcare & Clinical Trial Center, Chosun University) ;
  • Lee, Sook-Young (Marine Bio Research Center, Chosun University)
  • Received : 2022.02.10
  • Accepted : 2022.03.30
  • Published : 2022.03.31
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Abstract

In this study, conditions for extraction of functional component from Nypa fruticans wurmb was optimized. The yield by extraction with 30% ethanol (LE30, 42.12%) was higher than those hot water extraction (LDW 33.32%), 50% ethanol (LE50, 40.12%) and 70% ethanol (LE70, 34.5%). The extract was purified and analyzed by GC MS. The prevailing compounds found in extract were Cyclodecasiloxane-, pentadecanoic acid, -eicosane, undecanal and tridecanoic acid. The presence of saturated and unsaturated fatty acids in ethanolic extract vindicate the use of this plant to treat many diseases in traditional medicine. The total phenolic contents in the LDW, LE30, LE50, LE70 extract were 128±1.65 mg/g, 205±2.3 mg/g, 210±4.23 mg/g and 180±5.6 mg/g, respectively. The DPPH was highest in LE70 extract (1,000 ㎍/mL, 81.14%), ABTS was highest in LE50 extract (1,000 ㎍/mL, 84.14%). The protective effects against oxidative stress in raw 264.7 cell imparted by the LE50 extract was better than those imparted by the other extracts. The findings of the present study suggest that 50% ethanol is best solvent for extraction of Nypa fruticans Wurmb, considering yield, polyphenol content, and antioxidant activities with extraction cost.

Keywords

Acknowledgement

Following are results of a study on the "Leaders in INdustry-university Cooperation +" Project, supported by the Ministry of Education and National Research Foundation of Korea.

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