• Journal of Animal Science and Technology
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• v.64 no.1
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• pp.23-37
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• 2022

Thirty-six weaned piglets with an initial body weight (BW) of 8.43 ± 0.40 kg (28 days of age, ([Landrace × Yorkshire] × Duroc) were randomly assigned to 6 treatments for a 2-week feeding trial to determine the effects of different inorganic zinc (IZ), organic zinc (OZ) or combination of low crude protein diet (LP) and Mixed feed additive (MFA) on diarrhea score, nutrient digestibility, zinc utilization, blood profiles, organ weight, and fecal microflora in weaned piglet diet. The pigs were individually placed in 45 × 55 × 45 cm stainless steel metabolism cages in an environmentally controlled room (30 ± 1℃). The dietary treatments included a negative control (NC), positive control (PC; zinc oxide, 1,000 mg/kg), T1 (IZ : OZ, 850 : 150), T2 (IZ : OZ 700 : 300), T3 (IZ : OZ, 500 : 500), and T4 (LP + MFA [0.1% Essential oils + 0.08% Protease + 0.02% Xylanase]). The daily feed allowance was adjusted to 2.7 times the maintenance requirement for digestible energy (2.7 × 110 kcal of DE/kg BW^0.75). This allowance was divided into two equal parts, and the piglets were fed at 08 : 30 and 17 : 30 each day. Water was provided ad libitum through a drinking nipple. The diarrhea score was significantly increased (p < 0.05) in NC treatment compared with other treatments. The apparent total tract digestibility (ATTD) of dry matter (DM), nitrogen (N), and gross energy (GE) was significantly increased (p < 0.05) in the T2 treatment compared with the PC and NC treatments in week 1. In week 2, the ATTD of DM, N, and GE was significantly decreased (p < 0.05) in the NC treatment compared with other treatments. The T3 treatment had significantly higher (p < 0.05) ATTD and apparent ileal digestibility of zinc than the PC and T1 treatments. The Escherichia coli count in feces was significantly decreased in the T4 treatment compared with the NC and T2 treatments. The Lactobacillus count in feces was significantly increased in the T4 and T1 treatment compared with the T2 and T3 treatments. In conclusion, IZ : OZ 500 : 500 levels could improve nutrient digestibility and zinc utilization in weaned piglets, Moreover, MFA in LP diets could be used as a zinc alternative.

• ALGAE
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• v.37 no.2
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• pp.149-161
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• 2022

Noctiluca scintillans is a heterotrophic dinoflagellate that causes red-colored oceans during the day (red tides) and glowing oceans at night (bioluminescence). This species feeds on diverse prey, including phytoplankton, heterotrophic protists, and eggs of metazoans. Thus, many scientists have conducted studies on the ecophysiology of this species. It is easy to cultivate N. scintillans at a scale of <1 L, but it is difficult to cultivate them at a scale of >100 L because N. scintillans cells usually stay near the surface, while prey cells stay below the surface in large water tanks. To obtain mass-cultured N. scintillans cells, we developed an automatic system for cultivating N. scintillans on a scale of 100 L. The system consisted of four tanks containing fresh nutrients, the chlorophyte Dunaliella salina as prey, N. scintillans for growth, and N. scintillans for storage, respectively. The light intensities supporting the high growth rates of D. salina and N. scintillans were 300 and 20 µmol photons m^-2 s^-1, respectively. Twenty liters of D. salina culture from the prey culture tank were transferred to the predator culture tank, and subsequently 20 L of nutrients from the nutrient tank were transferred to the prey culture tank every 2 d. When the volume of N. scintillans in the predator culture tank reached 90 L 6 d later, 70 L of the culture were transferred to the predator storage tank. To prevent N. scintillans cells from being separated from D. salina cells in the predator culture tank, the culture was mixed using an air pump, a sparger, and a stirrer. The highest abundance of N. scintillans in the predator culture tank was 45 cells mL^-1, which was more than twice the highest abundance when this dinoflagellate was cultivated manually. This automatic system supplies 100 L of N. scintillans pure culture with a high density every 10 d for diverse experiments on N. scintillans.

• Journal of Animal Science and Technology
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• v.64 no.3
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• pp.564-573
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• 2022

In this study, considering the difficulties for all farms to convert farm styles to animal welfare-based housing, an experiment was performed to observe the changes in the behavior and welfare of sows when the slat floor was changed to a collective breeding ground. Twenty-eight sows used in this study were between the second and fifth parities to minimize the influence of parity. Using a flats floor cover, the flattening rates were treated as 0%, 20%, 30%, 40%, and 50%. Data collection was the behavior of sows visually observed using a camera (e.g., standing, lying, fighting and excessive biting behaviors, and abnormal behaviors) and the animal welfare level measured through field visits. Lying behavior was found to be higher (p < 0.01) as the flattening rate increased, and sows lying on the slatted cover also increased as the flattening rate increased (p < 0.01). Fighting behavior wasincreased when the flattening rate was increased to 20%, and chewing behavior was increased (p < 0.05) as the flattening rate increased. The animal welfare level of sows, 'good feeding', it was found that all treatment groups for body condition score and water were good at 100 (p < 0.05). 'Good housing' was the maximum value (100) in each treatment group. As the percentage of floor increased, the minimum good housing was increased from 78 in 0% flattening rate to 96 in 50% flattening rate. The maximum (100) 'good health' was achieved in the 0% and 20% flattening rates, and it was 98, 98, and 99 in the 30%, 50%, and 40% flattening rate, respectively. 'Appropriate behavior' score was significantly lower than that of other paremeters, but when the flattening ratio was 0% and 20%, the maximum and minimum values were 10. At 40% and 50%, the maximum values were 39 and 49, respectively, and the minimum values were analyzed as 19 for both 40% and 50%. These results will be used as basic data about sow welfare for farmers to successfully transition to group housing and flat floors.

• Journal of Nutrition and Health
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• v.56 no.3
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• pp.277-287
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• 2023

Purpose: This study was conducted to investigate the eating behavior (EB) and nutritional status of university students in Gwangju, and to compare their nutrition quotients (NQs) and problems according to their EB types. Methods: The subjects were 219 (127 female and 92 male) undergraduate students from Gwangju. The Student's t-test was used to compare the EB levels according to gender, and Pearson's χ² test was used to compare the distribution of EB types by gender. A gender-adjusted analysis of covariance (ANCOVA) was conducted to examine the difference in NQs by EB types. Results: First, there were significant differences in the EB scores and the distribution of EB types according to gender. Female students showed higher levels of emotional (Emo) and external (Ext) EBs than the male students, and Emo, Ext, and combined (Com) EB types were more evident among female rather than male students. Second, there was a difference in NQs according to the EB types. The Emo EB type subjects had a lower NQ than those of the restrained (Res) EB type and showed significant differences in diversity and dietary behavior. Specifically, the Emo EB type subjects displayed significantly lower efforts to maintain a healthy diet and subjective health status than the Com EB type and their levels of checking nutrition labels, hand washing, and water intake were significantly lower than that of the Res EB type. The Ext EB type showed significantly lower moderation of Ramyeon and sweetened drink intake than the Res and Com EB types. Conclusion: The level of EBs and the distribution of EB types varied according to gender, and the NQs were different according to EB types. The results of this study are expected to contribute to the establishment of customized strategies for improvement by identifying major nutritional problems by gender and the EB types of university students.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.26 no.6
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• pp.67-84
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• 2023

In some areas of the Republic of Korea, the designation and management of conservation areas do not adequately reflect regional characteristics and often impose behavioral regulations without considering the local context. One prominent example is the Busan EDC area. As a result, conflicts may arise, including large-scale civil complaints, regarding the conservation and utilization of these areas. Therefore, for the efficient designation and management of protected areas, it is necessary to consider various ecosystem factors, changes in land use, and regional characteristics. In this study, we specifically focused on the Busan EDC area and applied machine learning techniques to analyze the habitat of regional species. Additionally, we employed Explainable Artificial Intelligence techniques to interpret the results of our analysis. To analyze the regional characteristics of the waterfront area in the Busan EDC district and the habitat of migratory birds, we used bird observations as dependent variables, distinguishing between presence and absence. The independent variables were constructed using land cover, elevation, slope, bridges, and river depth data. We utilized the XGBoost (eXtreme Gradient Boosting) model, known for its excellent performance in various fields, to predict the habitat probabilities of 11 bird species. Furthermore, we employed the SHapley Additive exPlanations technique, one of the representative methodologies of XAI, to analyze the relative importance and impact of the variables used in the model. The analysis results showed that in the EDC business district, as one moves closer to the river from the waterfront, the likelihood of bird habitat increases based on the overlapping habitat probabilities of the analyzed bird species. By synthesizing the major variables influencing the habitat of each species, key variables such as rivers, rice fields, fields, pastures, inland wetlands, tidal flats, orchards, cultivated lands, cliffs & rocks, elevation, lakes, and deciduous forests were identified as areas that can serve as habitats, shelters, resting places, and feeding grounds for birds. On the other hand, artificial structures such as bridges, railways, and other public facilities were found to have a negative impact on bird habitat. The development of a management plan for conservation areas based on the objective analysis presented in this study is expected to be extensively utilized in the future. It will provide diverse evidential materials for establishing effective conservation area management strategies.

• Journal of Animal Science and Technology
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• v.52 no.2
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• pp.103-110
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• 2010

This study was conducted to determine the effect of supplemental medicinal plants (Artemisia, Acanthopanx and Garlic) on productive parameters in pigs. In experiment 1, a total of 90 multiparous sows were used in a 21-d performance study. The diets included: 1) CON (basal diet; Control), 2) BM1 (CON + 0.1% medicinal plants) and 3) BM2 (CON + 0.2% medicinal plants). Backfat thickness from farrowing to weaning was higher (P<0.05) in CON compared with sows fed treatments diets. The piglets weight gain was higher in the medicinal plants treatments (P<0.05). ADFI, nutrient digestibility and survivability were not affected by the experimental treatments. In experiment 2, a total of 60 finishing pigs (Landrace ${\times}$ Yorkshire ${\times}$ Duroc, $65.21\;{\pm}\;0.04\;kg$ average initial body weight) were used in a 56-d performance assay to determine the effects of supplemental medicinal plants (Artemisia, Acanthopanx and Garlic) on growth performance and carcass characteristics in finishing pigs. The dietary treatments included: 1) CON (basal diet ; Control), 2) BM1 (CON + 0.1% medicinal plants) and 3) BM2 (CON + 0.2% medicinal plants). For 4~8 weeks and overall period, ADG was higher (P<0.05) in the pigs fed medicinal plants. CON treatment was higher 24 pH loin and cooking loss than BM1 treatment (P<0.05). Water holding capacity and drip loss after 1day were affected by the dietary treatments (P<0.05). No numerical differences were observed in sensory evaluation, meat color, TBARS and loin area among three treatments. In conclusion, the results obtained from this feeding trial suggest that the medicinal plants mixture supplementation in diets for finishing pigs can improve ADG, water holding capacity, cooking loss and it improved backfat loss in sow and, litter weight gain.

• Korean Journal of Environment and Ecology
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• v.27 no.3
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• pp.305-315
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• 2013

This study is aimed to provide the preliminary data for conservation and management of wintering site for Anser fabalis population in Cheongna Area, Incheon Free Economic Zone, Korea through analyzing the relationship between the biotop structure of its wintering site and behavior characteristics. The main types (size and ratio) of the biotope in Cheongna Area, Incheon Free Economic Zone are reed wetlands ($6,093,762m^2$, 47.8%), rice fields without rice straw ($2,927,916m^2$, 23.0%), and rice fields with rice straw ($1,915,655m^2$, 15.0%). According to the survey carried on 13th Feb., 2013, total 33 species and 6, 535 individual birds were observed and among of them, the dominant bird was Anser fabalis showing 5,128 indiviuals, 78% of total population. As the result of analyzing the migratory route of Anser fabalis, the bird moved from roosting site to foraging site before and after sunrise and from feeding site to roosting site before and after sunset. According to the analysis of interrelation between habitat characteristics and biotope types of the bird, population density was the highest in reed wetlands among habitat types and individual appearance was the highest in water-filled rice field melting ice. The bird ate the roots and bulb of hydrophytes in reed wetlands and showed various behaviors like eating dropped grains, resting and sleeping in water-filled rice fields and eating dropped grains and resting in rice fields with and without rice straw. It is shown that the number of Anser fabalis appearing in rice fields is depended on the presence of dropped grains than types of rice field.

• Korean journal of applied entomology
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• v.47 no.4
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• pp.385-394
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• 2008

Larvae of Monochamus saltuarius showed normal growth and development on conifers of Pinus koraiensis, P. densiflora, Abies holophylla, Larix leptolepsis, P. bungeana, and P. rigida, respectively, but the conifers influenced significantly the body weight and the survival rate of larvae. Though the larval body weights were in a wide spectrum among treatments, growth curves of them were very similar from each other, showing continuous increase from the early larval stage to about 3 months old. The body weight was decreased slightly after the feeding period of the early 3 months. The size of larvae and adults became the largest from P. bungeana fed larvae. The mid-sized ones were from P. koraiensis, P. densiflora and A. holophylla. Small ones came from L. leptolepsis and P. rigida. The larval growth was retarded without water supply. Overall survival rates from the early stage of a larva to a fertile adult were 53.6% from P. koraiensis; 51.8%, P. densiflora; 34.7%, A. holophylla; 17.8%, P. bungeana; 16.7%, L. leptolepsis; and 12.3%, P. rigida. Adults from larvae fed the 6 species of conifers, respectively, were grown into the reproductively potent adults, which laid viable eggs. A few of overwintered larvae did not pupate and remained still as a larva until the late October of the year. Data from the field survey, the head width emerged from P. koraiensis was larger than that of L. leptolepsis. The adult emergence hole in P. koraiensis was larger also. While, the size of the emergence hole was larger in the artificially innoculated log of P. koraiensis, which was kept for a larva to be with a minimized food competition and sufficient water supply, than that of the field.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.5
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• pp.782-787
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• 2002

This study was done to investigate the effects of Korean mistletoe water extract and lectin on the apoptosis and preneoplastic lesion in chemically induced rat hepatocarcinogenesis. To attain the above objectives, weanling Sprague-Dawley male rats were fed modified AIN-76 diets containing 10% corn oil for 9 weeks. One week after feeding starts, rats were intraperitoneally injected twice with a dose of diethylnitrosamine (DEN, 50 mg/kg body weight (BW). Rats were provided with 0.05% phenobarbital (PB) in drinking water from one week after DEN treatment until the end of experiment. During the period of PB treatment, rats were injected with mistletoe extract (100 $\mu\textrm{g}$/kg BW) and lectin (10 $\mu\textrm{g}$/kg BW) twice a week. At the end of 9th week, rats were sacrificed and the formation of hepatic glutathione S-transferase placental form positive (GST-P$^{+}$) foci, apoptosis, DNA fragmentation and apoptosis related proteins were determined respectively. The formation of GST-P$^{+}$foci was significantly decreased by mistletoe extract or lectin treatment. Although there was no effect on apoptosis and DNA fragmentation in hepatic tissue by mistletoe extract or lectin treatment, caspase-9 and fas-L were increased. These results suggest that Korean mistletoe extract and lectin have a potential to inhibit hepatocarcinogenesis by increasing apoptosis.sis.

• Journal of Aquaculture
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• v.18 no.1
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• pp.19-25
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• 2005

This study was performed to investigate the optimum density for the intensive mass production of cyclopoid copepod, Paracyclopina nana in terms of nauplii and adults production. Effect of three development stages on the fecundity of adult female for nauplii production, survival rate of P. nana nauplii with different initial nauplii culture densities for adults production and cannibalistic feeding behavior of P. nana was examined, respectively. The fecundity of adult female by different female, copepodite ana nauplii density in 2 ml water volume decreased with the density of adult female, but was not affected by the density of either copepodite or nauplii. The average daily nauplii production for a adult female in 8 L water volume was $2.3{\times}10^5$ individuals with the incubation density of 7 adult females/ml, and this average value was significantly higher than those values of 0.6 to $1.7\times10^5$ individuals with the incubation density of 1,3,5, 10 adult females/ml (P<0.05). Survival rate of P. nana nauplil with different initial nauplii culture densities in 5 L vessels for 15 days were 32.7, 30.7, 28.9 and $23.0\%$ with the culture density of 50, 100, 150 and 200 inds./ml, respectively, but these were significantly higher than those of values 19.7 and $18.4\%$ with the culture density of 250 and 300 inds./ml (P<0.05). Cannibalistic behavior of P. nana adults on their offspring was observed, but the behavior decreased when phytoplankton was supplemented though there was no statistical difference (P>0.05). These results may indicate that P. nana is adaptable to the hatchery conditions and this species is cultured at the high densities. Optimum culture density for nauplii and adults production of P. nana were 7 adult females/ml and 200 nauplii/ml, respectively.