• Journal of Korean Society of Environmental Engineers
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• v.39 no.4
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• pp.214-219
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• 2017

We analyzed the water quality of first flush and rainfall runoff from a building rooftop, and investigated the removal of suspended solids (SS) in first flush using various media to develop a first flush filtration system. Particle size distribution exhibited most of particles in first flush from the rooftop ranged from 10 to 30 ${\mu}m$. SS concentrations maxed in 10~20 min and decreased afterwards. Dissolved organics and inorganic materials in runoff also showed highest levels in first flush (10 min). Filtration tests using anthracite (AC), polyurethane (PU), polypropylene (PP) showed about 50% of SS removal during the first 10 min operation, but the removal rates dramatically decreased after 20 min of filtration. Based upon the results from rinse and run cycle tests, only AC could achieve nice cycles without distinct decease of SS removal. SS removal rates increased with higher depth of media bed and lower flowrate. The system achieved over 50% of SS removal with a media depth of 30 cm and flowrate < 12 L/min.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.16 no.3
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• pp.315-330
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• 2018

A subcritical facility must ensure nuclear criticality safety under all circumstances. For this purpose, it is essential to have a procedure to validate that calculated values do not exceed upper subcritical limit (USL), determined by quantifying the bias and uncertainty. However, there are several validation methodologies of nuclear criticality safety and these can yield different USL. Therefore, it is necessary to analyze the validity of the methodologies to establish one methodology that can provide the most appropriate USL. In this study, two documents, a guide for validation of nuclear criticality safety calculational methodology (NUREG/CR-6698) and a criticality benchmark guide for light water reactor fuel in transport and storage package (NUREG/CR-6361), are compared and analyzed. In particular, the methodology in NUREG/CR-6361 is applied to the USLSTATS code. However, the analysis results show that the methodology in NUREG/CR-6698 is more appropriate, for several reasons. This is applied to decision of USL to design casks using SCALE code version 6.1.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.7-7
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• 2001

Cryopreservation by vitrification of Hanwoo blastocysts derived from nuclear transfer with Hanwoo adult ear cell was compared with that of in vitro fertilized blastocysts. For vitrification, day 7 or day 8 blastocysts were serially exposed in glycerol (G) or/and ethylene glycol (EG) mixtures (10% G for 5 min, 10% G plus 20% EG for 5 min, and 25% G plus 25% EG for 30 sec) which was diluted in 10% FBS added D-PBS. Thawing of straw was carried out in air for 10 sec and then in water bath of $25^{\circ}C$ for 20 sec. The contents of the straw (0.2 ml) was expelled into a culture dish contained with 0.5M sucrose and 10% FBS(S-DPBS) by cutting the cotton plug and then the recovered embryos were put into fresh 0.25M and 0.125M S-DPBS for 30 sec, respectively, The embryos were transferred into D-PBS with 10% FBS for 5 min. and were cultured in a 10u1 droplet of co-culture environment (cumulus cell monolayer + 10% added CRl medium) for 24 h. In the result, survival rates were 88.9% and 85.4% for nuclear transfer embryos and in vitro fertilized embryos, respectively. After transfer of vitrified-thawed blastocysts produced from nuclear transfer, 4 of 5 total recipients did not return to the subsequent estrus cycle at 30 days. It is concluded that the Hanwoo blastocysts derived from nuclear transfer can be successfully cryopreserved using simple and efficient vitrification method.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.387-393
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• 2011

The objective of this study was to develop a real-time PCR method for the rapid detection and quantification of the protozoan pathogen Perkinsus olseni using a TaqMan probe. For the standard, genomic DNA was extracted from $10^5$ in vitro-cultured P. olseni trophozoites, and then 10-fold serial dilutions to the level of a single cell were prepared. To test the reliability of the technique, triplicates of genomic DNA were extracted from $5{\times}10^4$ cells and 10-fold serial dilutions to the level of 5 cells were prepared. The standards and samples were analyzed in duplicate using an $Exicycler^{TM}$ 96 real-time quantitative thermal block. For quantification, the threshold cycle ($C_T$) values of samples were compared with those obtained from standard dilutions. There was a strong linear relationship between the $C_T$ value and the log concentration of cells in the standard ($r^2$ = 0.996). Detection of DNA at a concentration as low as the equivalent of a single cell showed that the assay was sensitive enough to detect a single cell of P. olseni. The estimated number of P. olseni cells was similar to the original cell concentrations, indicating the reliability of P. olseni quantification by real-time PCR. Accordingly, the designed primers and probe may be used for the rapid detection and quantification of P. olseni from clam tissue, environmental water, and sediment samples.

• The Korean Journal of Malacology
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• v.26 no.1
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• pp.97-105
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• 2010

Annual gonad development of the rock shell, Thais clavigera distributed in Jeju Port was investigated over a 12 month period from March 1998 to February 1999. Monthly change in gonad development was examined using histology. Gametogenesis of T. clavigera in the study area initiated as early as in October and fully ripe eggs could be observed from May to July. Percent gonad area (PGA) also increased rapidly from May to July then dropped in August when the water temperature remained $22.6-24.5^{\circ}C$, suggesting that rock shell released their eggs during this period. All female rock shell collected during the course of study exhibited pseudo-penis in the head, indicating that a certain level of imposex had progressed in the study site. Relative penis size index (RPSI), an indicator of level of imposex phenomenon varied 59.5-173.4% and this value was relatively higher than the index reported from elsewhere. It was believed that such imposex phenomenon observed in T. clavigera was caused by endocrine disruption by chemical contaminants such as TBTs released from biocidal paints in the port environment.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.6
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• pp.2883-2888
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• 2014

Background: Realgar which contains arsenic components has been used in traditional Chinese medicine (TCM) as an anticancer drug. However, neither Realgar nor its formula are soluble in water. As a result, high dose of Realgar has to be administered to achieve an effective blood medicine concentration, and this is associated with adverse side effects. The objective of the present study was to increase the solubility of a formula using hydrometallurgy technology as well as investigating its effects on in vitro and in vivo cell proliferation and apoptosis in Sarcoma-180 cell line. Materials and Methods: Antiproliferative activity of Realgar Bioleaching Solution (RBS) was evaluated by MTT assay. Further, effects of RBS on cell proliferation and apoptosis were studied using flow cytometry and transmission electron microscopy. Kunming mice were administered RBS in vivo, where arsenic specifically targeted solid tumors. Results: The results indicated that RBS extract potently inhibited the tumor growth of Sarcoma-180 cell line in a dose-dependent manner. Flow cytometry and transmission electron microscopy further indicated that RBS significantly induced cell apoptosis through the inhibition of cell cycle pathway in a dose-dependent manner. Further, on RBS administration to mice, arsenic was specifically targeted to solid tumor.s Conclusions: RBS could substitute for traditional Realgar or its formula to work as a potent tool in cancer treatment.

• Journal of the Korean Applied Science and Technology
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• v.36 no.4
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• pp.1427-1433
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• 2019

In this paper, Gas engine was tested for the energy of synthesis gas. As excess air ratio increase 1, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6 in 1800 rpm and synthesis gas, thermal efficiency generally decrease and power generation was 34 kWm at λ 1.4. And excess air ratio increase 1, 1.1, 1.2, 1.3, 1.4 in power generation 34 kWm, thermal efficiency generally increase 34.2%, 36.9%, 37.2%, 37.4%, 38.1%. Total efficiency through power generation consumes 38.7 kg/h of fuel at 30 kWe load and recovers 57.3% of waste heat by recovering 57.3 kW of waste heat through 32.1% power generation efficiency and heat recovery from cooling water and exhaust gas. The total efficiency was 85.8%.

• Proceedings of the Korea Contents Association Conference
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• 2009.05a
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• pp.759-764
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• 2009

With its rapid growth, the digital contents-related industry is drawing attention as an industry generating a high value added. Specifically, the 3D morphing technology often used in the appearance or movement of each object comprising contents makes it possible to compose various contents by inferring the appearance and movement of the object. For a realistic swimming appearance of fish by using fish that swim in the water, this paper proposes a technique that applies the same speed concept as a physical pendulum to a swimming cycle and applies a frame morphing technique that is able to express the growth process from fry to adult fish. This article is capable of realistically reviving the fish growth process, and can through magnified applying can be applied to in 3D fishes encyclopedias or virtual underwater expressions etc. in the viewpoint which is oceanography.

• KIEAE Journal
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• v.16 no.1
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• pp.67-71
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• 2016

Purpose: There is a growing interest in rainwater runoff reduction effect of green roof, as flooding caused by increasing impervious surface is becoming more and more frequent in urban areas. This study was conducted to prove runoff reduction and runoff delay effect of the retentive green roof and to investigate its influencing factors to the rainfall events that occurred in the summer of 2013. Method: The experiment intended to monitor the runoff quantity of the retentive green roof($140m^2$) and normal roof($100m^2$) in #35 building in Seoul National University, Seoul, Korea for 75 days in 2013. Result: On analysis of 9 rainfall events, it showed that the retentive green roof has 24.8~100% of runoff reduction ratio, 21.2~100% of peak flow reduction ratio, 0.5~3.75 hours of peak delay, and $1.8{\sim}7.2m^3$ of retaining capacity in an area of $140m^2$. It shows different results depending on rainfall and antecedent dry days. The results show that runoff reduction effect is effective when the rainfall is less than 50 mm and antecedent dry day is longer than five days on average. By installing retentive green roofs on buildings, it can help mitigate urban floods and rehabilitate urban water cycle.

• Journal of Plant Biology
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• v.17 no.4
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• pp.143-156
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• 1974

The purpose of this experiment was to study one side of germination physiology based on that protein profiles and protease relating to protein metabolism, that peroxidase, catalase, $\alpha$-amylase, $\beta$-amylase, and malate dehydrogenase involved in the carbohydrate metabolism of seed germination. All these experiments were divided into the two groups with and without acetone treatment, and were carried out. The protein bands of each germinating stage between the groups treated with and without acetone showed certain basic pattern in polyacrylamide gel disc electrophoresis. However, there was a little difference in the number of protein band, optical density, and migration velocity between two groups. The isozyme bands of peroxidase, and catalase between two groups in polyacrylamide gel disc electrophoresis did not show the numeral difference, but the optical density of certain germinating stage treated with acetone was higher than the group untreated with it and it showed their enzyme activity. The $\alpha$-amylase and $\beta$-amylase activities which involved in starch metabolism of seed germination were higher in the treated group than the other. On one hand, the protease activity of hydrolase occurred in the seeds for germination was also higher, more or less in the treated group than in the other. The isozyme band pattern of malate dehydrogenase in TCA cycle of energy metabolism pathway was very different between two groups growing for 72 hours with and without acetone treatment in cellulose acetate electrophoresis. It indicated that two isozyme bands of malate dehydrogenase was high. Consequently these experimental results mentioned above indicated that acetone treatment before sowing had an effect on dissolving certain complexed lipid substance involved in the seed coats, the activity of carbohydrate hydrolase increased with water absorption which was most comfortable in its germination, dissolved glycerin and fatty acid became certain energy source, and they stimulated the acceleration of respiration metabolism.