• Journal of Biosystems Engineering
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• v.28 no.5
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• pp.429-438
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• 2003

This study exploits the robot system which is necessary in gene study and bio-technology industry. As well, a DNA chip, which of use has been increased recently, can be manufactured with this system. The robot consists of a device spotting DNA on the silylated slide, a well plate, a bed for fixing well plates, devices of washing and drying the pin in DNA spotting .device, a distillation-water vessel, and a discharge vessel of wash water. We made the period of sticking DNA to the pin on the well plate to be 15 seconds. The spot size of DNA was set to be 0.28 mm on the average by bringing the slide into contact with pin during 1 second. If DNA is spotted in minimum space possible about 0.32mm, this system can stick about 8,100 DNA spots on the well plate with this rate. Analyzing the procedure: Movement starts, Pin washes, dries, and smears DNA on the well plate. Spotting DNA onto 12 chips took 2 minutes and 50 seconds.

• Journal of Environmental Health Sciences
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• v.31 no.1
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• pp.73-78
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• 2005

A determination method of trace Ni(II) and Zn(II) in aqueous solution was studied and developed by adsorbing on titanium dioxide. For this purpose, several conditions were optimized such as the pH of sample solution, adsorption time, the types and concentration acid, and desorption time. The titanium dioxide was added in sample solution which was pH adjusted. Then, the sample solution was stirred for 5 minutes. This mixture was stored in room temperature for 30 minutes to allow adsorption. After filtering and washing the titanium dioxide, the analytes were dissolved from the titanium dioxide on membrane filter by an ultrasonic vibration for 10 minutes in 1.0 M $HNO_3$ solution. Then, this sample solution was analysed using ICP-AES. The adsorption equilibrium was achieved in 30 minutes. The desorption was the most of effective with 1.0 M(mol/l) nitric acid solution, and desorption time was 10 minutes. This procedure was applied for the analysis of two real samples, i.e., brown seaweed and tangle. The recoveries of Ni(II) and Zn(II) in spiked samples were 89.4${\sim}$98.9% for analytes.

• Korean Journal of Microbiology
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• v.14 no.4
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• pp.176-185
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• 1976

An improved procedure for the rapid purification of glucose-6-phosphate dehydrogenase from extracts of Saccharomyces cerevisiae was developed by using affinity chromatography. Among six affinty media tested, NADP$^{+}$-agarose and Affi-gel Blue were more effective than others (i.e., Affi-gel Red, AMP-agarose, ATP-agarose, and NAD$^{+}$-agarose). Conditions to desorb the enzyme bound to the affinity media were examined to increase the purity as well as yield. The best result was obtained when the column was developed with a linear gradient of KCl (0-1.0M). In case of Affi-gel Blue, introduction of NAD$^{+}$ (15mM) washing step prior to the salt gradient was most effective to remove NAD$^{+}$-binding proteins. For a large scale preparation of G-6-P dehydrogenase higher recovery was obtained by Affi-gel Blue than NADP$^{+}$-agarose, however, the purity of the enzyme was decreased by 10 times if the former was used as the affinity medium. The capacity of Affi-gel Blue for G-6-P dehydrogenase was found to be 5 times higher than that of NADP$^{+}$-agarose. Furthermore Affi-gel Blue could be reused repeatedly and its preparation is relatively easier and less expensive than NADP$^{+}$-agarose.X> +/-agarose.

• Korean Chemical Engineering Research
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• v.43 no.6
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• pp.696-703
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• 2005

The definition of cake is not established for cake filtration, and especially the definition was impossible for the filtration of the floc already sedimented. The definition is proposed with the experimental method named 'filtration-permeation'. The limit of water content which can be achieved with cake filtration of floc was established with the definition of cake. The expression operation of which the purpose is to reduce the water content of pre-formed filter cake is calculated with our 'unified theory on solid-liquid separation' and compared with the experimental results. The importance of expression is analyzed by the calculated whole procedure of cake filtration and expression. The method determining the most effective operational conditions of filter press including the cake discharge and washing time is proposed.

• Journal of Korean Society on Water Environment
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• v.20 no.6
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• pp.664-669
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• 2004

An upflow BAF(Biological Aerated Filter) equipped with an expanded clay media was applied to reuse weaving wastewater of water jet loom. The performance of lab-scale biofilter was investigated by the adjustment of EBCTs(Empty Bed Contact Time) and the packing ratio of media, which were changed 1.1 to 3.7hr and 38 to 63%, respectively. In most conditions except 1.1hr of EBCT, BOD, CODcr, SS and Turbidity of the effluent were 1~4mg/L, 7~16mg/L, 1~5mg/L and 5~14NTU, where their removal efficiencies were 76~95%, 82~93%, 63~94% and 59~81%, respectively. From the observation of SEM(Scanning Electron Microscope) photographs of porous clay media, it was revealed that this media provided good performance of retaining microbes effectively. In addition, $0.44~0.49kgVSS/kgBOD_{rem}$. of low sludge reduction was expected. The most efficient back washing cycle and procedure were once per 4 to 9 days and air including collapse-pulsing method, respectively. Therefore, this system can be of use as an weaving wastewater treatment for reuse.

• Textile Coloration and Finishing
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• v.13 no.2
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• pp.18-18
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• 2001

This study has examined the removal of mixed soil of protein and fat by protease. Cotton and PET fabrics were soiled by spotting of hemoglobin and triolein, respectively. The soiling order and soil concentration were changed in this procedure. The soiled fabrics were aged at 130℃ for 20 minutes. Protease was added in the alcohol ethoxylate(AE) detergent solution. The removal effciency was evaluated by analysis of protein and/or fat on the fabrics before and after washing, respectively. The detergency of PET fabrics was higher than that of cotton fabrics. The removal efficiency of hemoglobin was improved by protease from cotton and PET fabrics. Especially the removal efficiency of hemoglobin was remarkably improved from cotton fabrics. With the increase of hemoglobin and triolein (1:1) mixed soil, the removal of mixed soil was increased in proportion to mixed soil content up to a certain point. but it began to decrease above the point from cotton fabrics, while it was generally increased from PET fabrics. The detergency of total mixed soil from cotton fabrics was higher in case of soiling order with triolein after hemoglobin than in case of soiling order with triolein before hemoglobin. But the soiling order was not greatly effected in the detergency of total mixed soil from PET fabrics.

• Textile Coloration and Finishing
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• v.13 no.2
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• pp.104-113
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• 2001

This study has examined the removal of mixed soil of protein and fat by protease. Cotton and PET fabrics were soiled by spotting of hemoglobin and triolein, respectively. The soiling order and soil concentration were changed in this procedure. The soiled fabrics were aged at $130^\circ{C}$ for 20 minutes. Protease was added in the alcohol ethoxylate(AE) detergent solution. The removal effciency was evaluated by analysis of protein and/or fat on the fabrics before and after washing, respectively. The detergency of PET fabrics was higher than that of cotton fabrics. The removal efficiency of hemoglobin was improved by protease from cotton and PET fabrics. Especially the removal efficiency of hemoglobin was remarkably improved from cotton fabrics. With the increase of hemoglobin and triolein (1:1) mixed soil, the removal of mixed soil was increased in proportion to mixed soil content up to a certain point. but it began to decrease above the point from cotton fabrics, while it was generally increased from PET fabrics. The detergency of total mixed soil from cotton fabrics was higher in case of soiling order with triolein after hemoglobin than in case of soiling order with triolein before hemoglobin. But the soiling order was not greatly effected in the detergency of total mixed soil from PET fabrics.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 1993.10a
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• pp.12-19
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• 1993

In general, the line balancing problem is defined as of finding an assignment of the given jobs to the workstations under the precedence constraints given to the set of jobs. Usually, the objective is either minimizing the cycle time under the given number of workstations or minimizing the number of workstations under the given cycle time. In this paper, we present a new type of an assembly line balancing problem which occurs in an electronics company manufacturing home appliances. The main difference of the problem compared to the general line balancing problem lies in the structure of the precedence given to the set of jobs. In the problem, the set of jobs is partitioned into two disjoint subjects. One is called the set of fixed jobs and the other, the set of floating jobs. The fixed jobs should be processed in the linear order and some pair of the jobs should not be assigned to the same workstations. Whereas, to each floating job, a set of ranges is given. The range is given in terms of two fixed jobs and it means that the floating job can be processed after the first job is processed and before the second job is processed. There can be more than one range associated to a floating job. We present a procedure to find an approximate solution to the problem. The procedure consists of two major parts. One is to find the assignment of the floating jobs under the given (feasible) assignment of the fixed jobs. The problem can be viewed as a constrained bin packing problem. The other is to find the assignment of the whole jobs under the given linear precedence on the set of the floating jobs. First problem is NP-hard and we devise a heuristic procedure to the problem based on the transportation problem and matching problem. The second problem can be solved in polynomial time by the shortest path method. The algorithm works in iterative manner. One step is composed of two phases. In the first phase, we solve the constrained bin packing problem. In the second phase, the shortest path problem is solved using the phase 1 result. The result of the phase 2 is used as an input to the phase 1 problem at the next step. We test the proposed algorithm on the set of real data found in the washing machine assembly line.

• Korean Journal of Agricultural Science
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• v.30 no.1
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• pp.76-88
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• 2003

This study exploits the robot system which is necessary in gene study, bio-technology industry. As well, it can achieve the job of DNA chip manufacturing whose use rate has been increased recently. The robot consists of DNA spotting device for spotting DNA on the silylated slide and well plate, bed for fixing well-plate, washing & drying device of washing and drying the pin part of DNA spotting device, distillation-water vessel, and discharge vessel of wash water. We made the term of sticking DNA to the pin on well plate to be 15 seconds. The spot size of DNA was set to be 0.28 mm on the average by bringing the slide into contact with pin for 1 second. At this rate, if DNA is spotted in the minimum space possible of about 0.32mm, it can stick about 8,100 DNA spots on the well plate. Analyzing the procedure: Movement starts. Pin washes, dries, and smears DNA on the well plate. Spots DNA onto 12 chips takes 2 minutes and 50 seconds.

• Tuberculosis and Respiratory Diseases
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• v.40 no.1
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• pp.29-34
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• 1993

Background: The development of the flexible fiberoptic broncoscope by Ikeda was an important technologic advance in the diagnosis and management of patients with pulmonary disease. But, cross contamination related to fiberoptic bronchoscope was reported in cases involving tubercle bacilli, MOTT and other agents. Therefore, cleaning and disinfecting of fiberoptic bronchoscope requires careful attention. Methods: From September 1991 to May 1992, medical records of all patients with positive culture for MOTT in bronchial washing specimens were reviewed. Also to evaluate bactericidal effect of 2% glutaraldehyde, culture was performed after inoculum of MOTT, Serratia marsescens and Pseudomonas aeruginosa to the disinfectant solution. Results: In 2% alkaline glutaraldehyde, MOTT was not survived only after 30 minute exposure, but P. aeruginosa and S. marsescens were rapidly inactivated with no survivors after exposure to 2% glutaraldehyde. Since vigorous mechanical cleansing and more than 30 minute of contact time within washing machine, no more outbreak was observed. Conclusions: It is also very important that bronchoscopes must be meticulously cleaned after each procedure and more than 30 minute exposure would be required for eradication of MOTT with 2% glutaraldehyde. However even the most strictly applied infection control measures cannot exclude contamination completly and clinicians have to stay alert to this possibility. Prompt detection of pseudoepidemics is possible if abrupt increase in isolation rates, especially if they involve unusual or generally nonpathogenic organisms, are readily recognized.