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http://dx.doi.org/10.5307/JBE.2003.28.5.429

Development of Microarrayer for Manufacturing DNA Chip  

이현동 (Dept. of Agricultural Machinery Engineering, Chungnam National University)
김기대 (Agricultural Machinery Engineering, Chungnam National University)
나건영 (Agricultural Machinery Engineering, Chungnam National University)
임용표 (Horticuture science. Chungnam National University)
Publication Information
Journal of Biosystems Engineering / v.28, no.5, 2003 , pp. 429-438 More about this Journal
Abstract
This study exploits the robot system which is necessary in gene study and bio-technology industry. As well, a DNA chip, which of use has been increased recently, can be manufactured with this system. The robot consists of a device spotting DNA on the silylated slide, a well plate, a bed for fixing well plates, devices of washing and drying the pin in DNA spotting .device, a distillation-water vessel, and a discharge vessel of wash water. We made the period of sticking DNA to the pin on the well plate to be 15 seconds. The spot size of DNA was set to be 0.28 mm on the average by bringing the slide into contact with pin during 1 second. If DNA is spotted in minimum space possible about 0.32mm, this system can stick about 8,100 DNA spots on the well plate with this rate. Analyzing the procedure: Movement starts, Pin washes, dries, and smears DNA on the well plate. Spotting DNA onto 12 chips took 2 minutes and 50 seconds.
Keywords
DNA chips; Microarrayer; DNA spotting; Genome project;
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  • Reference
1 이상엽, 윤성호, 임근배, 조윤경. 2000. Genomics와 DNA chip. New & Information for chemical engineerings. Vol. 18(3):307-311.
2 이현동, 김기대, 김찬수, 김성환, 나건영, 임용표. 2002. 유전자 검색을 위한 DNA chip 제작용 로봇 시스템의 개발(II) - 로봇 시스템 성능실험 -. 한국농업기계학회 2002년 동계학술대회 논문집 Vol. 7(2):333-338.
3 Huibin, Y. etc. 2001. An evaluation of the performance of cDNA microarrays for detecting changes in global mRNA expression. Nucleic Acids Research Vol. 29(8).   DOI   ScienceOn
4 Brown, T. A. 1987. Gene Clonning. Chapman & hall.
5 Yoon, S. H., J. G. Choi and S. Y. Lee. 2000. Development of DNA chip microarrayer. J. Microbiol. Biotechnol. Vol. 10(1):21-26.   과학기술학회마을
6 이현동, 김기대, 임용표, 김찬수. 2002. 유전자 검색을 위한 DNA chip 제작용 로봇 시스템의 개발(I) - 국내외 연구동향 -. 한국농업기계학회 2002년 동계학술대회 논문집 Vol. 7(1):407-412.   과학기술학회마을
7 Cheung, V. G., M. Morley, F. Aguilar, A. Massimi, R. Kucherlapati and G. Childs. 1999. Making and reading microarrays. Nat. Gen. 21:15-19.   DOI   ScienceOn
8 이성우. 2000. DNA 칩의 최근동향. 공학교육과 기술. Vol. 7(3):53-56.