• 제목/요약/키워드: Washing procedure

검색결과 93건 처리시간 0.027초

먹장어 어묵 제조 (PROCESSING OF STEAMED FISH JELLY PRODUCT FROM HAGFISH)

  • 김수현
    • 한국수산과학회지
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    • 제11권4호
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    • pp.197-203
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    • 1978
  • 먹장어를 연제품원료로서 이용하기 위한 기초자료를 얻고자 먹장어 어묵의 최적가공조건을 실험한 결과를 요약하면 다음과 같다. 채육한 육에 대하여 전분은 $5\%$, 식염은 $3\%$, tripolyphosphate는 $0.3\%$, 수세회수는 6회, 1단가열은 $45\~50^{\circ}C$에서 1시간, 2단가열은 $90^{\circ}C$에서 30분간이 좋았다. 1단가열 대신 자연응고 현상을 이용할 경우에는 $25^{\circ}C$에서 1시간 자연응고 시킨 다음 $90^{\circ}C$에서 30분간 가열하는 것이 좋았다.

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중요민속자료(복식)의 보존처리 (The Conservation Treatment for the Important Folklore Materials-Clothes)

  • 한성희;이규식
    • 보존과학연구
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    • 통권14호
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    • pp.94-108
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    • 1993
  • The cultural properties of cloth are of animal orgin (silk), or of vegetable orgin(cotton, hemp, ramie). As clothes are of an orginic material, they were subjected to damage by chemical, phisigical or biological factors, viz, moulds insects, lights, humidity and temperature changes, etc. And these factors promote that clothes generally result from various types of deterioration. In 1992, We were performed the conservation treatments for total 9 pieces of cloth, such as 3 pieces of General PAK SHIN-RYONG(Important Folklore Material No.110) 3 pieces of Madam Jung(Important Folklore Material No.115) and 1 piece of King Se-jo(Important Folklore Material No.219). The procedure of the conservation treatment for clothes describe the following below. 1) The washing and dry-cleaning to remove the contaminated substances from cloth was used 0.2% stearyl potassium soap solution and the mixture solution compound of n-Hexane, C6H14. and n-Decane, C10H22. And after the washing and dry-cleaning, the dry of clothes was carried out in a warm condition. These steps were repeated in 2 times over for each cloth. 2) The repair of clothes was attached the similar textiles to stronger fabric linings by needlework.3) The reprodution was made for cloth of King Se-jo to equalize the type, color, quality and skill of materials. 4) After these above procedures, all clothes fumigated to prevent the biodeterioration by using the mixed gas of methyl bromide and ethylene oxide as insecticide and fungicide. 5) Finally for the purpose to keep in a safety long-term condition, the treated clothes sealed with Biaxially Oriented Polyvinylacohol Film(BO-PVA film) and Helium, purity 99.999%, filled up in sealed BO-PVA film bag.

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환원/확산 공정에 의한 고성능 Nd-Fe-B 자성분말의 제조 (Preparation of Highly Efficient Nd-Fe-B Magnetic Powders by Reduction/Diffusion Process)

  • 김동수;진춘강;백연경;최철진
    • 한국분말재료학회지
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    • 제20권3호
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    • pp.197-202
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    • 2013
  • A novel route to prepare Nd-Fe-B magnetic particles by utilizing both spray drying and reduction/diffusion processes was investigated in this study. Precursors were prepared by spray drying method using the aqueous solutions containing Nd salt, Fe salt and boric acid with stoichiometric ratios. Precursor particles could be obtained with various sizes from 2 to $10{\mu}m$ by controlling concentrations of the solutions and the average size of $2{\mu}m$ of precursors were selected for further steps. After heat treatment of precursors in air, Nd and Fe oxides were formed through desalting procedure, followed by reduction processes in Hydrogen ($H_2$) atmosphere and with Calcium (Ca) granules in Argon (Ar) successively. Moreover, diffusion between Nd and Fe occurred during Ca reduction and $Nd_2Fe_{14}B$ particles were formed. With Ca amount added to particles after $H_2$ reduction, intrinsic coercivity was changed from 1 to 10 kOe. In order to remove and leach CaO and residual Ca, de-ionized water and dilute acid were used. Acidic solutions were more effective to eliminate impurities, but Fe and Nd were dissolved out from the particles. Finally, $Nd_2Fe_{14}B$ magnetic particles were synthesized after washing in de-ionized water with a mean size of $2{\mu}m$ and their maximum energy product showed 9.23 MGOe.

현미(玄米)의 도정(搗精) 및 취반(炊飯) 과정중 BHC 잔류분(殘留分)의 제거(除去) (Elimination of BHC Residues in the Polishing and Cooking Processes of Brown Rice)

  • 김용화;김혜남;김상순;이서래
    • 한국식품과학회지
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    • 제11권1호
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    • pp.18-25
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    • 1979
  • BHC를 살포하여 재배한 현미(玄米)(BHC잔류량 0.3 ppm)의 도정(搗精), 세미(洗米), 및 취반(炊飯)중 BHC 잔류량(殘留量)을 분석하여 그의 행방을 추적한 결과는 다음과 같다. 1) 현미의 도정(搗精)중 BHC는 10분도미에서 8%, 7분도미에서 20%가 잔류하였다. 2) 쌀의 세척(洗滌)중 10분도미에서는 34%, 7분도미에서는 31%가 잔류하였다. 3) 재래식 취반(炊飯)과 전기밥솥에 의한 취반중 10분도미에서는 각각 86%, 77%, 7분도미에서는 각각 69%, 41%가 잔류하였다. 4) 전체적으로 보아 현미로부터 밥으로의 BHC 이행률(移行率)은 10분도미에서 2.3%, 7분도미에서 4.3%에 불과하였다. 따라서 현미의 조리(調理), 가공(加工)중 발생되는 BHC의 제거량(除去量)을 잔류농약 허용량(許容量)설정에 반영(反映)하여 야 될 것이다.

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영구자석 동기 전동기의 I-F 구동과 센서리스 구동을 위한 속도 제어 절환 기법 (Speed Controller Transition Method for I-F Operation and Sensorless Operation of Permanent Magnet Synchronous Motor)

  • 김동욱;김성민
    • 전기전자학회논문지
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    • 제23권2호
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    • pp.543-551
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    • 2019
  • 영구자석 동기 전동기는 출력 밀도가 높고 효율이 높다는 장점 때문에 적용 범위가 넓어지고 있다. 자동차나 로봇과 같은 고전력밀도, 고성능 전동기 구동 시스템뿐만 아니라 세탁기, 에어컨, 냉장고와 같은 비용 절감이 매우 중요한 시스템에도 영구자석 동기 전동기가 사용되고 있다. 비용 절감을 위해 회전자 위치 센서를 제거하는 센서리스 제어가 필요한데, 일반적으로 센서리스 제어는 전동기를 기동하는 조건에서는 사용하기 어렵다. 따라서 초기 기동에서는 전류 벡터를 임의의 속도로 회전시키는 I-F 속도 제어를 사용하고, 특정 속도 이상이 되면 센서리스 속도 제어로 절환해야 한다. I-F 속도 제어와 센서리스 속도 제어에서의 속도 제어 성능도 중요하지만 두 제어 기법이 절환되는 과도 상태에서도 속도 제어 성능을 유지해야 한다. 본 논문에서는 영구자석 동기 전동기의 센서리스 속도 제어를 위해 I-F 속도 제어에서 센서리스 속도 제어로의 절환 기법을 제안한다. 제안된 기법의 성능을 확인하기 위해 세탁기 구동 시스템에서 실험을 수행하였다.

Rapid Staining Using the Shorr Method for Intraoperative Peritoneal Washing Cytology in Advanced Gastric Cancer: a Pilot Study from a Single Institution

  • Son, Sang-Yong;Choi, Hai-Young;Lee, Yoontaek;Park, Young Suk;Shin, Dong Joon;Oo, Aung Myint;Jung, Do Hyun;Ahn, Sang-Hoon;Park, Do Joong;Lee, Hye Seung;Kim, Hyung-Ho
    • Journal of Gastric Cancer
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    • 제19권2호
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    • pp.173-182
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    • 2019
  • Purpose: Intraoperative peritoneal washing cytology (PWC) is used to determine treatment strategies for gastric cancer with suspected serosal invasion. However, a standard staining method for intraoperative PWC remains to be established. We evaluated the feasibility of a rapid and simple staining method using Shorr's stain for intraoperative PWC in advanced gastric cancer. Materials and Methods: Between November 2012 and December 2014, 77 patients with clinical T3 or higher gastric cancer were enrolled. The sensitivity, specificity, and concordance between the Shorr staining method and conventional Papanicolaou (Pap) staining with carcinoembryonic antigen (CEA) immunohistochemistry (IHC) were analyzed. Results: Intraoperative PWC was performed laparoscopically in 69 patients (89.6%). The average time of the procedure was 8.3 minutes, and the average amount of aspirated fluids was 83.3 mL. The average time for Shorr staining and pathologic review was 21.0 minutes. Of the 77 patients, 16 (20.7%) had positive cytology and 7 (9.1%) showed atypical findings; sensitivity and specificity were 73.6% and 98.2% for the Shorr method, and 78.9% and 98.2% for the Pap method with CEA IHC, respectively. Concordance of diagnosis between the 2 methods was observed in 90.9% of cases (weighted ${\kappa}$ statistic=0.875) and most disagreements in diagnoses occurred in atypical findings (6/7). In overall survival, there was no significant difference in C-index between the 2 methods (0.459 in Shorr method vs. 0.458 in Pap with CEA IHC method, P=0.987). Conclusions: Shorr staining could be a rapid and reliable method for intraoperative PWC in advanced gastric cancer.

국내 소아에서 최초로 초음파기관지내시경-세침흡인술을 이용하여 진단된 흉곽 내 결핵 림프절염 증례 (The First Pediatric Case of Intrathoracic Tuberculosis Lymphadenitis Diagnosed by Endobronchial Ultrasound Guided Transbronchial Needle Aspiration)

  • 김광훈;이경종;김예진
    • Pediatric Infection and Vaccine
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    • 제20권3호
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    • pp.186-189
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    • 2013
  • EBUS-TBNA는 성인에서 폐암의 진단 및 병기 결정에 중요한 역할을 하고 있는 시술로서 최근 흉곽 내 결핵 림프절염의 진단에 있어서의 역할에 대한 연구도 이루어지고 있는 중이나 아직 소아에 있어서 이 방법을 통한 진단은 보고된 바가 없는 상태이다. 저자들은 병력 및 피부 결핵 반응 검사, 흉부 CT 소견을 통해 결핵 림프절염이 의심되는 13세 여아에서 EBUS-TBNA를 통한 조직학적 진단 1례를 경험하였기에 보고하는 바이다. 환자는 3주간 지속된 발열 주소로 입원하여 진단적 검사 진행 중 피부 결핵반응 강양성 소견 보였고 흉부 CT상 종격동 및 폐문 주위의 커져 있는 림프절 소견 보여 이 부위에 대한 EBUS-TBNA 시행 후 결핵 림프절염 진단 후 총 6개월간의 항결핵제 치료를 완료하였다.

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소 수정란의 전염성질병 예방 (Infectious Disease Control of Bovine Embryos)

  • 석호봉
    • 한국수정란이식학회지
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    • 제1권1호
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    • pp.16-27
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    • 1986
  • Based on the current importing and exporing regulations for disease control of embryo transfer, some important microorganisms and their control possibilities are reviewed. The results reviewed were sumrnarized as follows: 1. Regulations regarding to the import of embryos vary between importing and exporting countries, but exporting countries examine the donor and embryos for the heaith certification by the requirements of importing countries. 2. Organisms that infect the gametes are 5 kinds of viruses and the diseases caused by them could not be controlled or eradicated using embryo transfer. 3. Organisms that do not infect the gametes are 4 kinds of viruses and the causal organisms are potential candidates for control or eradication by embryo transfer. 4. Organisms that penetrate the zona pellucida and infect the embryo are 6 kinds of viruses including bovine viral diarrhea virus. 5. Organisms that cannot penetrate the zona pellucida or do not infect the embryo are 15 kinds of viruses and the removal from their contaminations are recommended by proper washing procedure and antisera treatment. Bovine and porcine parvovirus, porcine pseudorabies virus and vesicular stomatitis virus are included in these organisms. 6. Bovine embryos that artificially exposed to various pathogenic organisms such as bovine herpes virus, IBR virus, bluetongue virus, bovine viral diarrhea virus and Brucella abortus in vitro are discussed about their infection by several treatments.

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Comparative study of 2-nitroimidazole-fluorophore-conjugated derivatives with pimonidazole for imaging tumor hypoxia

  • Seelam, Sudhakara Reddy;Hong, Mi Kyung;Lee, Yun-Sang;Jeong, Jae Min
    • 대한방사성의약품학회지
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    • 제5권2호
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    • pp.101-112
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    • 2019
  • Herein, 2-nitroimidazole-fluorophore conjugates were synthesized by linking 2-nitroimidazole and FITC or RITC via thiourea bonds. The prepared derivatives were stable for 2 h in Dulbecco's modified Eagle's medium (DMEM) at 37 ℃. The novel conjugates were studied for their in vitro uptake under hypoxic conditions using U87MG and CT-26 cell lines, showing significantly higher uptakes in hypoxic than normoxic cells. Immunohistochemical analysis confirmed hypoxia in U87MG and CT-26 xenografted tumor tissues. Moreover, the prepared conjugates were evaluated by in vivo experiments after intravenous injection in U87MG and CT-26 xenografted mice. Hypoxia was confirmed by immunohistochemistry of the prepared derivatives with co-injected pimonidazole. Confocal microscopy of the prepared derivatives showed strong fluorescence in hypoxic tumor tissues correlated with the pimonidazole distribution. This suggested that the 2-nitroimidazole-fluorophore conjugates are promising optical imaging probes for tumor hypoxia and are promising substitutes for pimonidazole immunohistochemistry, which requires a multi-step procedure of incubation involving antibody, second antibody, dye, hydrogen peroxide, and multiple washing steps.

콜로니 픽킹 로봇 시스템의 개발 (II) - 로봇 시스템의 성능실험 - (Development of Robot System for Colony Picking (II) - Performance test of the robot system -)

  • 이현동;김기대;나건영;이영규
    • Journal of Biosystems Engineering
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    • 제28권5호
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    • pp.449-456
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    • 2003
  • A robot system was developed to pick desired colony. This robot system consisted of an image acquisition process which acquires the image information of colony, an illumination device which irradiates the object once when it gets the image of it, a picking head, a replicating head, a bed for fixing well-plates, and a sterilization device of sterilizing pin stained with colony. Picking pins were washed in an ultrasonic wave washing for takes ten seconds. Picking pins were dipped for sterilizing in alcohol for ten seconds. The time for resterilizing picking pins in a heater was five seconds. This performance test resulted 100% success rate for both the colony picking and the colony replication process. Considering the procedure in which 8 pins were smeared by colony, picked on a well plate, and sterilized as one cycle, the system could carry out one cycle in 110 seconds. It took about 138 minutes to pick around 600 colonies.