• Title/Summary/Keyword: Washing procedure

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PROCESSING OF STEAMED FISH JELLY PRODUCT FROM HAGFISH (먹장어 어묵 제조)

  • KIM Soo-Hyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.11 no.4
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    • pp.197-203
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    • 1978
  • In this study, the effects of some additives to the quality of fish jelly product such as salt tripolyphosphate and starch were examined. Besides, that of washing and stepwise heating procedure were also discussed. From the result of quality test by measuring jelly strength and sensory evaluation the product was quite palatable 19 common, and the addition of 3 percent of salt and 5 percent of starch resulted in the best jelly strength. It was markedly enforced when 0.3 percent of tripolyphosphate was added. The suggestible processing method of hagfish jelly product is as follows : The meat is to be bleached by washing at least 6 times with tenfold tap water by volume of meat at $5-6^{\circ}C$ and then ground throughly with addition of 3 percent of salt, 5 percent of starch and 0.3 percent of tripolyphosphate. The ground meat is finally to be heated for 1 hour at 45 to $50^{\circ}C$ and then for 30 minutes at $90^{\circ}C$.

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The Conservation Treatment for the Important Folklore Materials-Clothes (중요민속자료(복식)의 보존처리)

  • Han, Sung-Hee;Lee, Kyu-Shik
    • 보존과학연구
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    • s.14
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    • pp.94-108
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    • 1993
  • The cultural properties of cloth are of animal orgin (silk), or of vegetable orgin(cotton, hemp, ramie). As clothes are of an orginic material, they were subjected to damage by chemical, phisigical or biological factors, viz, moulds insects, lights, humidity and temperature changes, etc. And these factors promote that clothes generally result from various types of deterioration. In 1992, We were performed the conservation treatments for total 9 pieces of cloth, such as 3 pieces of General PAK SHIN-RYONG(Important Folklore Material No.110) 3 pieces of Madam Jung(Important Folklore Material No.115) and 1 piece of King Se-jo(Important Folklore Material No.219). The procedure of the conservation treatment for clothes describe the following below. 1) The washing and dry-cleaning to remove the contaminated substances from cloth was used 0.2% stearyl potassium soap solution and the mixture solution compound of n-Hexane, C6H14. and n-Decane, C10H22. And after the washing and dry-cleaning, the dry of clothes was carried out in a warm condition. These steps were repeated in 2 times over for each cloth. 2) The repair of clothes was attached the similar textiles to stronger fabric linings by needlework.3) The reprodution was made for cloth of King Se-jo to equalize the type, color, quality and skill of materials. 4) After these above procedures, all clothes fumigated to prevent the biodeterioration by using the mixed gas of methyl bromide and ethylene oxide as insecticide and fungicide. 5) Finally for the purpose to keep in a safety long-term condition, the treated clothes sealed with Biaxially Oriented Polyvinylacohol Film(BO-PVA film) and Helium, purity 99.999%, filled up in sealed BO-PVA film bag.

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Preparation of Highly Efficient Nd-Fe-B Magnetic Powders by Reduction/Diffusion Process (환원/확산 공정에 의한 고성능 Nd-Fe-B 자성분말의 제조)

  • Kim, Dongsoo;Chen, Chunqiang;Baek, Younkyoung;Choi, Chuljin
    • Journal of Powder Materials
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    • v.20 no.3
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    • pp.197-202
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    • 2013
  • A novel route to prepare Nd-Fe-B magnetic particles by utilizing both spray drying and reduction/diffusion processes was investigated in this study. Precursors were prepared by spray drying method using the aqueous solutions containing Nd salt, Fe salt and boric acid with stoichiometric ratios. Precursor particles could be obtained with various sizes from 2 to $10{\mu}m$ by controlling concentrations of the solutions and the average size of $2{\mu}m$ of precursors were selected for further steps. After heat treatment of precursors in air, Nd and Fe oxides were formed through desalting procedure, followed by reduction processes in Hydrogen ($H_2$) atmosphere and with Calcium (Ca) granules in Argon (Ar) successively. Moreover, diffusion between Nd and Fe occurred during Ca reduction and $Nd_2Fe_{14}B$ particles were formed. With Ca amount added to particles after $H_2$ reduction, intrinsic coercivity was changed from 1 to 10 kOe. In order to remove and leach CaO and residual Ca, de-ionized water and dilute acid were used. Acidic solutions were more effective to eliminate impurities, but Fe and Nd were dissolved out from the particles. Finally, $Nd_2Fe_{14}B$ magnetic particles were synthesized after washing in de-ionized water with a mean size of $2{\mu}m$ and their maximum energy product showed 9.23 MGOe.

Elimination of BHC Residues in the Polishing and Cooking Processes of Brown Rice (현미(玄米)의 도정(搗精) 및 취반(炊飯) 과정중 BHC 잔류분(殘留分)의 제거(除去))

  • Kim, Yong-Hwa;Kim, Hye-Nam;Kim, Sang-Soon;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.11 no.1
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    • pp.18-25
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    • 1979
  • In order to investigate the effect of polishing washing and cooking processes on the residue level of BHC in rice grain, brown rice samples having a 0.3 ppm total BHC content were subjected to various treatments and residue analysis. The results are summarized as follows: 1) The conventional polishing process of brown rice decreased the residue level down to 8 and 20% for 100 and 70% polished rice, respectively. 2) The washing procedure decreased the level to 34 and 31% for 100 and 70% polished rice, respectively. 3) The cooking processes with a conventional kettle and an automatic electric cooker decreased the residue level to 86 and 77% in 100% polished rice, and 69 and 41% in 70% polished rice, respectively. 4) By summation of the above results in sequence. it was concluded that the residue levels of BHC in cooked rice were 2.3 and 4.3% of original residue in the brown rice for 100 and 70% polished rice, respectively.

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Speed Controller Transition Method for I-F Operation and Sensorless Operation of Permanent Magnet Synchronous Motor (영구자석 동기 전동기의 I-F 구동과 센서리스 구동을 위한 속도 제어 절환 기법)

  • Kim, Dong-Uk;Kim, Sungmin
    • Journal of IKEEE
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    • v.23 no.2
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    • pp.543-551
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    • 2019
  • Permanent Magnet Synchronous Motors(PMSMs) have a wider range of applications due to their high output density and high efficiency. PMSMs are used not only in high-power density, high-performance motor-driven systems such as vehicle and robots, but also in systems where cost-cutting is very important, such as washing machines, air conditioners and refrigerators. To reduce costs, position sensorless control is required, which is generally difficult to be used under conditions of starting the motor. Thus, the I-F speed control that rotates the current vector at any speed in the starting procedure should be used at first, and then the sensorless speed control could be applied after PMSM rotates above a certain speed. Speed control performance in I-F speed control and sensorless speed control is very important. And more speed control performance should be maintained even in the transient in which the two control techniques are changed. In this paper, the speed controller transition method from I-F speed control to sensorless speed control of permanent magnet synchronous motor is proposed. Experiments were carried out on the washing machine drive system to verify the performance of the proposed technique.

Rapid Staining Using the Shorr Method for Intraoperative Peritoneal Washing Cytology in Advanced Gastric Cancer: a Pilot Study from a Single Institution

  • Son, Sang-Yong;Choi, Hai-Young;Lee, Yoontaek;Park, Young Suk;Shin, Dong Joon;Oo, Aung Myint;Jung, Do Hyun;Ahn, Sang-Hoon;Park, Do Joong;Lee, Hye Seung;Kim, Hyung-Ho
    • Journal of Gastric Cancer
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    • v.19 no.2
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    • pp.173-182
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    • 2019
  • Purpose: Intraoperative peritoneal washing cytology (PWC) is used to determine treatment strategies for gastric cancer with suspected serosal invasion. However, a standard staining method for intraoperative PWC remains to be established. We evaluated the feasibility of a rapid and simple staining method using Shorr's stain for intraoperative PWC in advanced gastric cancer. Materials and Methods: Between November 2012 and December 2014, 77 patients with clinical T3 or higher gastric cancer were enrolled. The sensitivity, specificity, and concordance between the Shorr staining method and conventional Papanicolaou (Pap) staining with carcinoembryonic antigen (CEA) immunohistochemistry (IHC) were analyzed. Results: Intraoperative PWC was performed laparoscopically in 69 patients (89.6%). The average time of the procedure was 8.3 minutes, and the average amount of aspirated fluids was 83.3 mL. The average time for Shorr staining and pathologic review was 21.0 minutes. Of the 77 patients, 16 (20.7%) had positive cytology and 7 (9.1%) showed atypical findings; sensitivity and specificity were 73.6% and 98.2% for the Shorr method, and 78.9% and 98.2% for the Pap method with CEA IHC, respectively. Concordance of diagnosis between the 2 methods was observed in 90.9% of cases (weighted ${\kappa}$ statistic=0.875) and most disagreements in diagnoses occurred in atypical findings (6/7). In overall survival, there was no significant difference in C-index between the 2 methods (0.459 in Shorr method vs. 0.458 in Pap with CEA IHC method, P=0.987). Conclusions: Shorr staining could be a rapid and reliable method for intraoperative PWC in advanced gastric cancer.

The First Pediatric Case of Intrathoracic Tuberculosis Lymphadenitis Diagnosed by Endobronchial Ultrasound Guided Transbronchial Needle Aspiration (국내 소아에서 최초로 초음파기관지내시경-세침흡인술을 이용하여 진단된 흉곽 내 결핵 림프절염 증례)

  • Kim, Kwang Hoon;Lee, Kyung Jong;Kim, Yae-Jean
    • Pediatric Infection and Vaccine
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    • v.20 no.3
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    • pp.186-189
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    • 2013
  • Endobronchial ultrasound guided transbronchial needle aspiration (EBUS-TBNA) now provides an important alternative diagnostic modality in patients with intrathoracic tuberculosis lymphadenopathy. The procedure is well tolerated in the outpatient setting, provides access to the mediastinal and hilar lymph node locations commonly in tuberculosis and also allows bronchial washing to be performed at the same procedure. However, there is no report of EBUS-TBNA applied to children to diagnose tuberculosis. We report a case of EBUS-TBNA applied to children who had intrathoracic tuberculosis lymphadenopathy.

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Infectious Disease Control of Bovine Embryos (소 수정란의 전염성질병 예방)

  • 석호봉
    • Journal of Embryo Transfer
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    • v.1 no.1
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    • pp.16-27
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    • 1986
  • Based on the current importing and exporing regulations for disease control of embryo transfer, some important microorganisms and their control possibilities are reviewed. The results reviewed were sumrnarized as follows: 1. Regulations regarding to the import of embryos vary between importing and exporting countries, but exporting countries examine the donor and embryos for the heaith certification by the requirements of importing countries. 2. Organisms that infect the gametes are 5 kinds of viruses and the diseases caused by them could not be controlled or eradicated using embryo transfer. 3. Organisms that do not infect the gametes are 4 kinds of viruses and the causal organisms are potential candidates for control or eradication by embryo transfer. 4. Organisms that penetrate the zona pellucida and infect the embryo are 6 kinds of viruses including bovine viral diarrhea virus. 5. Organisms that cannot penetrate the zona pellucida or do not infect the embryo are 15 kinds of viruses and the removal from their contaminations are recommended by proper washing procedure and antisera treatment. Bovine and porcine parvovirus, porcine pseudorabies virus and vesicular stomatitis virus are included in these organisms. 6. Bovine embryos that artificially exposed to various pathogenic organisms such as bovine herpes virus, IBR virus, bluetongue virus, bovine viral diarrhea virus and Brucella abortus in vitro are discussed about their infection by several treatments.

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Comparative study of 2-nitroimidazole-fluorophore-conjugated derivatives with pimonidazole for imaging tumor hypoxia

  • Seelam, Sudhakara Reddy;Hong, Mi Kyung;Lee, Yun-Sang;Jeong, Jae Min
    • Journal of Radiopharmaceuticals and Molecular Probes
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    • v.5 no.2
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    • pp.101-112
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    • 2019
  • Herein, 2-nitroimidazole-fluorophore conjugates were synthesized by linking 2-nitroimidazole and FITC or RITC via thiourea bonds. The prepared derivatives were stable for 2 h in Dulbecco's modified Eagle's medium (DMEM) at 37 ℃. The novel conjugates were studied for their in vitro uptake under hypoxic conditions using U87MG and CT-26 cell lines, showing significantly higher uptakes in hypoxic than normoxic cells. Immunohistochemical analysis confirmed hypoxia in U87MG and CT-26 xenografted tumor tissues. Moreover, the prepared conjugates were evaluated by in vivo experiments after intravenous injection in U87MG and CT-26 xenografted mice. Hypoxia was confirmed by immunohistochemistry of the prepared derivatives with co-injected pimonidazole. Confocal microscopy of the prepared derivatives showed strong fluorescence in hypoxic tumor tissues correlated with the pimonidazole distribution. This suggested that the 2-nitroimidazole-fluorophore conjugates are promising optical imaging probes for tumor hypoxia and are promising substitutes for pimonidazole immunohistochemistry, which requires a multi-step procedure of incubation involving antibody, second antibody, dye, hydrogen peroxide, and multiple washing steps.

Development of Robot System for Colony Picking (II) - Performance test of the robot system - (콜로니 픽킹 로봇 시스템의 개발 (II) - 로봇 시스템의 성능실험 -)

  • 이현동;김기대;나건영;이영규
    • Journal of Biosystems Engineering
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    • v.28 no.5
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    • pp.449-456
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    • 2003
  • A robot system was developed to pick desired colony. This robot system consisted of an image acquisition process which acquires the image information of colony, an illumination device which irradiates the object once when it gets the image of it, a picking head, a replicating head, a bed for fixing well-plates, and a sterilization device of sterilizing pin stained with colony. Picking pins were washed in an ultrasonic wave washing for takes ten seconds. Picking pins were dipped for sterilizing in alcohol for ten seconds. The time for resterilizing picking pins in a heater was five seconds. This performance test resulted 100% success rate for both the colony picking and the colony replication process. Considering the procedure in which 8 pins were smeared by colony, picked on a well plate, and sterilized as one cycle, the system could carry out one cycle in 110 seconds. It took about 138 minutes to pick around 600 colonies.