• Title/Summary/Keyword: Wash water rate

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Development of Microarrayer for Manufacturing DNA Chip (DNA 칩 제작을 위한 로봇 시스템의 개발)

  • 이현동;김기대;나건영;임용표
    • Journal of Biosystems Engineering
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    • v.28 no.5
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    • pp.429-438
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    • 2003
  • This study exploits the robot system which is necessary in gene study and bio-technology industry. As well, a DNA chip, which of use has been increased recently, can be manufactured with this system. The robot consists of a device spotting DNA on the silylated slide, a well plate, a bed for fixing well plates, devices of washing and drying the pin in DNA spotting .device, a distillation-water vessel, and a discharge vessel of wash water. We made the period of sticking DNA to the pin on the well plate to be 15 seconds. The spot size of DNA was set to be 0.28 mm on the average by bringing the slide into contact with pin during 1 second. If DNA is spotted in minimum space possible about 0.32mm, this system can stick about 8,100 DNA spots on the well plate with this rate. Analyzing the procedure: Movement starts, Pin washes, dries, and smears DNA on the well plate. Spotting DNA onto 12 chips took 2 minutes and 50 seconds.

A Study on the Women's Behavior of Underwear Consumption (여성의 내의 소비 행동에 관한 연구)

  • 정혜원
    • Journal of the Korean Society of Clothing and Textiles
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    • v.28 no.3_4
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    • pp.422-432
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    • 2004
  • This study investigated underwear consumption behavior of women, who were 20∼59 years old and resided in Incheon during 2002 summer. 372 women completed questionnaires, and data were analyzed using factor analyses, t-test, ANOVA, $\chi$$^2$-analyses. They usually purchased underwear considering comfort and economic factor at a discount store. They wore sleeveless T-shirts, but they didn't wear underpants except panties highly all the year round. Higher rate of them wore pantyhose instead of underpants during autumn, winter and spring. They demanded unstitching of stitches and lengthened sleeves in most of the groups. And older and lowly educated women group wanted widen armhole of upper underwear, and tighten waistband of panties. They generally washed in washing machine using cold water. Besides they added oxygen bleach and softner during wash and final rinse. Older than 20's boil their underwear frequently. The women in a group of boiling commonly answered the looseness of waistband. It is recommended not to boil but to soak with bleach added warm water for improving the whiteness and durability of underwear. For a treatment with higher temperature deprives the elasticity of spandex.

A Study on Estimating Diffuse Pollution Loads Removal by Road Vacuum Cleaning (도로청소에 의한 비점오염부하 삭감량 산정방법 연구)

  • Lee, Taehwan;Cho, Hong-Lae;Jeong, Euisang;Koo, Bhon K.;Park, Baekyung;Kim, Yongseok
    • Journal of Korean Society on Water Environment
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    • v.33 no.2
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    • pp.123-129
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    • 2017
  • The purpose of this study is to identify potential methodologies to reasonably estimate the effectiveness of road vacuum cleaning in terms of pollution loads reduction. In this context, this study proposes two empirical equations to estimate the amount of diffuse pollution loads removed by road vacuum cleaning. The proposed equations estimate the removed amount of pollution loads respectively taking into consideration of: a) the distance of road vacuum cleaning; and b) the amount of road-deposited sediment(RDS). All of the parameters in these equations were evaluated based on results of field monitoring and laboratory analyses, except for the RDS generation rate. The results of this study suggest that pollutant removal efficiency is 46.3% for $BOD_5$ and 56.4% for TP; discharge ratios for particulate and dissolved $BOD_5$ are 35.0% and 21.2%, respectively; discharge ratios for particulate and dissolved TP are 35.0% and 19.4%, respectively. Average concentrations of pollutants in RDS are $BOD_5$ 977.3 mg/kg and TP 317.6 mg/kg. Some results of a case study imply that both equations can be potentially useful if the adopted parameters are reasonably evaluated. In particular, the RDS generation rate should be evaluated based on monitoring data collected from various road conditions.

A Study on the Literature Review of Acorn in Korea (도토리에 대한 국내의 연구 동향)

  • 김복남
    • Korean journal of food and cookery science
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    • v.11 no.2
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    • pp.158-163
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    • 1995
  • This study was surveyed and compiled the contribution about acorn in korea .the physico-chemical properties of acorn and acorn starch. preparation of acorn starch and elimination of the acorn tannin, the rheological properties of acorn starch gel. Nutrition of acorn and antioxidative activity of tannin in acorn extract. The result obtained were as follow. 1. The chemical compositions of acorn were water 6.5∼13.7%, crude ash 1.9∼3.4%, crude fat 1.1∼5.0%, crude protein 5.8∼7.8%, crude fiber 2.1∼3.6%, N-free extract 71∼77.5%, total tannin 4.6∼9.3%, Ca 92.7∼460.9mg%, p 80.0∼740.9mg%, Na 66.2∼93.9 mg%, and K 867.9∼983.1mg%. 2. The acorn tannin was extracted with water, acetone, and ethanol. The generation method was wash SE settling method with water. 3. The shape of acorn starch granule was rounded triangular and some elliptical, rasing power 12.4∼12.5, Blue value 0.43∼0.47, Alkali number 10.8∼11.3, Amount of Arnylose 28.8∼30.50% Tannin contents on the initial go tim-zation temperature of acorn starch were not influenced but maximum and cooling viscosity o the acorn starch were decreased. 4. Nutritional effect and diet absorption rate were not influence, by rice and 20% T-A(elimination of tannin from the acorn) mixed diet, and the content of lipid in s rum were not influenced by rice 40% and T-A (elimination of tannin from the acorn) mixed diet. 5. jallic acid, digallic acid, gallotannin were contained in acorn powder extract. The main antioxidative : stlvity was speculated due to the gallic acid.

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Removal of Pesticide Residues in Field-sprayed Leafy Vegetables by Different Washing Method (엽채류에 엽면 살포된 농약의 세척 방법에 따른 제거)

  • Kwon, Hyeyoung;Kim, Taek-Kyum;Hong, Su-Myeong;Kim, Chan-Sub;Baeck, Minkyeong;Kim, Doo-Ho;Son, Kyung-Ae
    • The Korean Journal of Pesticide Science
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    • v.17 no.4
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    • pp.237-243
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    • 2013
  • Pesticides were sprayed on perilla leaf and leafy lettuce in a greenhouse and the reduction rate of pesticide residues on each vegetable by washing were tested. The reduction rate of pesticide residues by washing for 30 sec~3 min on perilla leaf were 3~63% in tap water, 2~58% in salt water, 6~74% in green tea water, and 8~86% in detergent solution. The detergent solution only showed significant difference in reduction rates compared to the tab water washing. Considering reduction effects of the washing duration, it was showed that the reduction rates were a pattern of inclining as the duration of washing process increased, but there was no significant difference in the reduction rates except the reduction rates between washing in the detergent solution for 1 min and 3 min. Comparing washing in flowing tab water and in stagnant tab water with leafy lettuce, the reduction rate by one time washing were 8~68% in flowing tab water and 7~64% in stagnant tab water. The water and the time used in this experiment were 17.5 L, 2.9 min with flowing tab water and 4 L, 1 min with stagnant tab water. The reduction rate by 3 times washing in stagnant tab water were 16.5~76.6%, and the water and the time used were 12 L, 3 min. Therefore, when the water and the time used to wash vegetables were considered, washing two or three times in stagnant tab water could be more effective than washing one time in flowing tab water.

Separation of Caffeine and Catechin Compounds from Green Tea by Quercetin Molecular Imprinted Solid-Phase Extraction (케르세틴 분자각인 고정상 추출을 이용한 녹차에서 카페인 및 카테킨 화합물의 분리)

  • Jin, Yin-Zhe;Row, Kyung-Ho
    • Journal of the Korean Chemical Society
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    • v.51 no.2
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    • pp.165-170
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    • 2007
  • In this work, caffeine and some catechin compounds such as +C, EGC and EGCG were extracted from green tea using quercetin molecular imprinted polymers in solid-phase extraction. For synthesis of MIP, quercetin as the templates, MAA as the monomer, EGDMA as the crosslinker and AIBN as the initiator were used. For extraction of caffeine and catechin compounds from green tea, the solid-phase extractions of a load followed by wash and elution procedures were done with water, methanol and methanol:acetic acid=90:10 (vol.%) as the solvents, respectively. HPLC analysis (C18 column, 5 μm, 250×4.6 mm) with the mobile phase of methanol:water=40:60 (vol.%) at a flow rate of 0.5 ml/min was adopted for the quantitative determination. By solid-phase extraction, the resolutions of caffeine and some catechin compounds from green tea were increased. The quercetin-MIP had higher selectivity to +C compounds.

Characteristics of Non-Point Pollution from Road Surface Runoff

  • Lee, Chun-Sik;Jang, Seong-Ho
    • Journal of Environmental Science International
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    • v.19 no.6
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    • pp.665-670
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    • 2010
  • Pollutants from urban pavement consists various kinds of substances which are originated from dry deposition, a grind out tire, corrosive action of rain to pavement and facilities and raw materials of the road etc.. These are major pollutants of urban NPS (Non-point source) during rainfall period. However there is not enough information to control such pollutants for appropriate management of natural water quality. In this study of transportation areas, three monitoring stations were set up at trunk road, urban highway and national road in Gyeongnam province. Runoff flow rate was measured at every 15minutes by automatic flow meters installed at the end of storm sewer pipe within the road catchment area for water quality analysis. Data was collected every 15 minutes for initial two hours of rainfall. Additional samples were collected 1-4 hours interval till the end of rainfall. The monitoring parameters were $COD_{Mn}$, SS, T-N & T-P and heavy metals. The average EMCs of TSS and $COD_{Mn}$ were 62.0 mg/L and 24.2 mg/L on the city trunk road, which were higher than those of urban highway and national road, indicating higher pollutant loads due to activities in the city downtown area beside the vehicle. On the other hand, the average EMC of T-N and T-P were in the range of 2.67-3.23 mg/L and 0.19-3.21 mg/L for all the sampling sites. Heavy metals from the roads were mainly Fe, Zn, Cu and Mn, showing variable EMCs by the type of road. From the TSS wash-off analysis in terms of FF(first flush) index, first flush phenomenon was clearly observed in the trunk road(FF : 0.89-1.43). However, such mass delivery behavior was not apparently shown in urban highway(FF : 0.90-1.11) and national road(FF : 0.81-1.41).

A study on Runoff Characteristics of Non-point Pollutant with Rainfall Intensity - A case of fowls manure - (강우강도에 따른 비점오염원 유출 특성에 관한 연구 - 계분을 중심으로 -)

  • Lee, Min-Woo;Lee, Young-Shin
    • Journal of Wetlands Research
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    • v.11 no.1
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    • pp.91-97
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    • 2009
  • The fowls manure is using as fertilizers for farmland because of enough nutrients. However, excess nutrients can be washed off during a storm and affected on nearby waterbodies. In this paper, the runoff characteristics from farmland were studied to determine the washoff mass. A lab-scale reactor was designed to estimate the surface runoff and infiltration rates according to the rainfall intensity. Surface runoff water did not occur at 10mm/hr rainfall intensity, but some runoff occurred at 20mm/hr rainfall intensity. At 32.4mm/hr rainfall intensity, it shows the highest pollutant concentrations such as 686mg/L for $BOD_5$ and at 630mg/L $COD_{Mn}$. The pollutant as based on fowls manure compost was highly washed-off by subsurface water at 32.4mm/hr rainfall intensity, however the concentration was largely decreased at 43.2mm/hr rainfall intensity. The summary of the results is that the highest wash-off concentrations value shows at 32.4mm/hr rainfall intensity for $BOD_5$ and at 67.1mm/hr for T-N and T-P.

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Improvement of Methanogenic activity in the Anaerobic Treatment of Wastewater with High Sulfate (고농도 황산염을 함유한 폐수의 혐기성 처리시 메탄균의 효율 향상을 위한 연구)

  • Shin, Hang Sik;Oh, Sae Eun
    • Journal of Korean Society of Water and Wastewater
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    • v.10 no.2
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    • pp.47-54
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    • 1996
  • UASB reactor was operated for treating wastewater containing high sulfate to assess their performance, competition between sulfate-reducing bacteria(SRB) and methane-producing bacteria(MPB), and the change in the characteristics of microbial granules according to change of hydraulic retention time(HRT) in the reactor. The reactor was fed with a synthetic moderate strength wastes(glucose, 2000 mgCOD/l) containing high sulfate($2400mgSO_4{^{2-}}/l$). The organic loading rate(OLR) ranged from 1.5 to 3.0 gCOD/l.d as HRT maintained 15 to 30 hrs in the stage I. The COD removal efficiency was between 80 to 92%. During this period, methane yield rapidly decreased from 0.3 to 0.1 1 $CH_4$/gCODremoved. While sulfide concentration in the effluent increased from 80 to 200 mgS/l. This indicates that SRB becomes dominant over MPB at a relatively long HRT in the excess sulfate. When OLR of reactor maintained from 5 to 8 gCOD/l.d in the stage II, methane yield increased from 0.1 to 0.17 1 $CH_4$/gCODremoved regardless of decrease of COD removal efficiency. This indicates that SRB is more sensitive to the change of a short HRT than MPB. In the competition between SRB and MPB, about 30% of the removed COD was utilized by SRB at HRT of 30 hrs during the start-up period, while about 73% was used by SRB at HRT of 15hrs at the final step of second experimental stage. Whereas after shock exposure of OLR about 62% was utilized by SRB at HRT of 5hrs. It indicates that SRB is strongly suppressed by the wash-out of significant dispersed SRB since a large electron flow is distributed to the MPB. In addition, the granulation in the presence of high sulfate is unfavoured at a long HRT because of substrate transport limitations into MPB like Methanothrix spp. which is an important factor in the composition of the granules. Accordingly, granule sizes in the UASB reactor decreased with time due to weak network frame of granules by the decreased activity of MPB.

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Current conditions regarding dental infection management recognition of students in the department of dental hygiene (치위생(학)과 학생의 치과감염관리에 관한 인식현황)

  • Lee, Yeun-Kyoung;Kim, Soon-Duck
    • Journal of Korean society of Dental Hygiene
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    • v.9 no.3
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    • pp.468-478
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    • 2009
  • This research was performed to provide basic data for the development of infection related dental hygiene studies by surveying the current condition of recognition among students in the department of dental hygiene toward hospital infection management while receiving the following results by using a personal self-administered survey method targeting 303 students in the department of dental hygiene from certain areas. 1. With the current condition of recognition on the sanitization and sterilization of instruments among students in the department of dental hygiene(study), the rate at which surgical instruments are to be sterilized with autoclaves was 79.9% which was relatively higher than other instruments while it was shown that prosthetic instruments for treatment was 56.4%, conservative instruments for treatment was 51.8%, and ultrasonic scaler tip was 51.1% while the way syringe tips(36.1%) and the dental anesthetic apparatus(27.9%) were revealed to require sanitization by alcohol. 2. The 'hand wash' area was the highest with 4.71 while the 'materials and environment management' area and 'equipment management' area appeared high respectively with 4.43 and 4.41. 3. With the current condition of recognition on equipment management, 'equipments used for contagious patients are separately washed after a one-time use and must be sterilized or separated-and-discarded' was the highest with 4.82 while 'sterilization equipments with humidity or water on it are considered contaminated and are not used' showed the lowest recognition level with 3.90. 4. Regarding the current condition of materials and environment management, 'contagious and general trash are separated and discarded' was the highest with 4.70 while 'the refrigerator for medicine storage is cleaned on a regular basis once a month' was revealed as the lowest with 4.11. 5. With the current condition of recognition on hand washing, 'one must wash their hands after coming in contact with contagious patients, was the highest with 4.90 while washing hands after taking off gloves' appeared as the lowest with 4.51 point. To conclude department of dental hygiene there is to infection management and necessary about organization disinfecting and pasteurization to strengthen an education in order raising a stamp helping practical ratio about the infection management which whole, is from presence at a sickbed and connection does and about the infection management which is substantial and educational program development leads feed with the fact that deepening studying which is continuous must become accomplished becomes.

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