• Journal of Korean Medical Ki-Gong Academy
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• v.17 no.1
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• pp.24-51
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• 2017

Objective : The purpose of this study is to introduce Whidam's Su-Gi therapy(WST.) as a remarkable traditional manual therapy. Main subject : WST. is a traditional manual therapy from Do-In(導引) An-Kyo(按蹻) that treats disease by controlling Gi(Gi-Gong therapy). It makes treatment effects by pushing Sa-Gi(邪氣) which becomes aggregation-accumulation(積聚) to right places by using pointillage, stroking, and rubbing with doctor's focused intention(意念). The cervical spine connects body and brain, so treating cervical spine could be a foundation of all treatment. Discussion : WST. is a manual therapy that treats not only muscles, nerves, vessels, joints but also Oriental medicine concept-meridian, acupoint and Gi(氣). Conclusions: 1) WST. is a traditional manual therapy using Gi-Gong, 2) WST. changes Sa-Gi(邪氣) into Jung-Gi(正氣), which means curing aggregation-accumulation(積聚), 3) Cervical WST. treats a passage that connects upper and lower parts of human body; heart and brain, and 4) Cervical WST. is a treatment to activate So-Ju-Cheon(小周天), which controls human body immunity.

• Restorative Dentistry and Endodontics
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• v.35 no.4
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• pp.285-294
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• 2010

The purpose of this study was to evaluate the viability of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure by means of MTT assay, WST-1 assay. 12 teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both side of the first and second maxillary molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group 1 (Immediate extraction), group 2 (Slow freezing under pressure of 3 MPa), group 3 (Slow freezing under pressure of 2 MPa), group 4 (Slow freezing under no additional pressure), group 5 (Rapid freezing in liquid nitrogen under pressure of 2 MPa), group 6 (Rapid freezing in liquid nitrogen under no additional pressure), group 7 (low-temperature preservation at $0^{\circ}C$ under pressure of 2 MPa), group 8 (low-temperature preservation at $0^{\circ}C$ under no additional pressure), group 9 (low-temperature preservation at $-5^{\circ}C$ under pressure of 90 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in $37^{\circ}C$ water bath, then MTT assay, WST-1 assay were processed. One way ANOVA and Tukey HSD method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 7 ($0^{\circ}C$/2 MPa) showed higher viability of periodontal ligament cells than other group (2-6, 8) and this was statistically significant (p < 0.05), but showed lower viability than group 1, immediate extraction group (no statistical significance). By the results of this study, low-temperature preservation at $0^{\circ}C$ under pressure of 2 MPa suggest the possibility for long term preservation of teeth.

• Restorative Dentistry and Endodontics
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• v.34 no.4
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• pp.356-363
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• 2009

The purpose of this study was to evaluate the viability of periodontal ligament cells in rat teeth using slow cryo-preservation method under pressure by means of MTT assay and WST-1 assay. Eighteen teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both sides of the first and second maxillary molars were extracted as atraumatically as possible under Tiletamine anesthesia. The experimental groups were group 1 (Immediate control), group 2 (Cold preservation at $4^{\circ}C$for 1 week), group 3 (Slow freezing), group 4 (Slow freezing under pressure of 3 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in $37^{\circ}C$water bath, then MTT assay and WST-1 assay were processed. One way ANOVA and Tukey method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 4 showed significantly higher viability of periodontal ligament cells than group 2 and 3 (p < 0.05), but showed lower viability than immediate control group. By the results of this study, slow cryo-preservation method under pressure suggests the possibility for long term cryo-preservation of the teeth.

• Restorative Dentistry and Endodontics
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• v.34 no.6
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• pp.491-499
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• 2009

This study examined the influence of the storage methods on the viability of oral epithelial cells using conventional cell freezing storage, slow freezing preservation, rapid freezing preservation, and slow freezing preservation with a pressure of 2 Mpa or 3 Mpa. The cell viability was evaluated by cell counting, WST-1 and the clonogenic capacity after 6 days of freezing storage. After 6 days, the frozen cells were thawed rapidly, and the cell counting. WST-1, and clonogenic capacity values were measured and compared. 1. The results from cell counting demonstrated that conventional cryopreservation, slow freezing under a 2 Mpa pressure and slow freezing under a 3 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p < 0.05). 2. The results from the optical density by WST-1 demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p<0.05). 3. The clonogenic capacity demonstrated that slow freezing under a 2 Mpa pressure showed significantly higher values than slow freezing preservation and rapid freezing preservation (p < 0.05).

• Proceedings of the Korea Concrete Institute Conference
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• 1996.10a
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• pp.101-107
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• 1996

In this study, the wedge splitting test (WST) specimens with various strength levels were tested to investigate the fatigue crack growth behavior of concrete. Selected test variables were concrete compressive strength with 2 levels (28 MPa, 60 MPa, 100 MPa) and maximum fatigue loading with 2 levels (75%, 85%). Fatigue testing was preceded by fracture energy test and the crack growth was measured by means of the compliance calibration method, 60 WST specimens were cast for the fatigue test, and 6 companion cylinders ($\phi$100${\times}$ 200 mm) for each batch. In fatigue test, the frequency of loading cycle was 1 Hz, and the minimum fatigue loading level was 5~10 % of ultimate monotonic loading. On the basis of the experimental results, a fracture mechanics-based empirical relationships for fatigue crack growth rate (da/dN-$\Delta$KI relationships) were presented. In addition, the effect of initial notch depth on the fracture energy and the validity of compliance calibration technique for the WST were shown.

• Journal of the Ergonomics Society of Korea
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• v.33 no.2
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• pp.109-124
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• 2014

Objective: This study was conducted for one-hand users including hemiplegic clients currently using general folding manual wheelchairs, so as to analyze their specific problems and recommend solutions regarding usage. Background: Traditional manual wheelchairs require considerable use and control of both hands for operation, thus adaptations become necessary for individuals with asymmetrical use of hands. Method: Thirty hemiplegic clients who were admitted to rehabilitation and convalescent hospitals participated as subjects. The research tools were general folding manual wheelchairs commonly used by people with impaired gait, and the Wheelchair Skills Tests (WST) WST-M/WCU 4.1 version was adopted as the assessment tool. All participants were asked to fill out questionnaires on demographics and wheelchair usage characteristics. Assessment procedures were performed with currently used manual wheelchairs and with/without the use of foot to control the wheelchair. Results: When the participants drove folding manual wheelchairs without the use of foot, even the lowest failure rate among the WST items tested recorded 96.7%. On the contrary, with the use of foot in maneuvering the wheelchairs, failure rates dropped noticeably and success rate among the WST items tested was as high as 86.7%. Conclusion: These findings imply that the use of one-arm (hand) propellable (drivable) wheelchair can be an active and effective solution in resolving problems for hemiplegic clients using existing manual wheelchairs. As such, the government should provide institutional support to further develop and distribute this device or technology, and promote relative research in tandem. For now, the supply of commercially available device to hemiplegic clients is deemed urgent and also a mechanism to provide the devices and relevant services. Application: This study offers viable solutions for hemiplegic clients who rely on existing manual wheelchairs to increase their mobility and occupational performance.

• Journal of fish pathology
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• v.28 no.2
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• pp.71-78
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• 2015

In this study, we conducted to the development of a rapid antimicrobial susceptibility test method using WST-1 which is known to water-soluble tetrazolium salt, in order to rapidly response against bacterial diseases in fish. Eight of antibiotics which are permissioned for marine organism from government were used to rapid antimicrobial susceptibility testing using the WST-1. As a result, a similar tendency was verified compare to conventional antibiotic susceptibility test results. Generally, the antibiotic susceptibility test method required about 3 days (72 hours) for determine the effective antibiotics, however, we have confirmed that the our method using WST-1 was required at least 36 hours in this study. Consequentially, our method will contribute to development of rapid antimicrobial susceptibility testing for bacterial diseases in fish.

• Journal of Korean Electronics
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• v.7 no.1
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• pp.60-61
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• 1987

• Journal of Sensor Science and Technology
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• v.32 no.6
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• pp.412-417
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• 2023

There is increasing interest in the rapid and highly sensitive monitoring of cell viability in biological and toxicological research. Conventional methods depend on optical assays using Water Soluble Tetrazolium-8 (WST-8) or 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, which requires a large volume of samples and special instruments, necessitating shipment of clinical samples to laboratories. This paper reports on the development of a rapid and sensitive electrochemical (EC) sensor using screen printed electrode (SPE) and surface modification using 4'-mercapto-N-phenylquinone diamine (4'-NPQD), as double electron mediators, for monitoring cell viability via the measurement of nicotinamide adenine dinucleotide (NADH). We used the sensor to observe the viability of MCF-7 and doxorubicin (Dox)-treated cells. The oxidation current of NADH was measured via chronoamperometry (CA), and the EC results showed a good linear relationship when compared with NADH quantification using WST-8 assay. The analysis time was only 10 s and limit of detection (LOD) of NADH was 1.78 µM. Our EC method has the potential to replace conventional WST assays for cell viability and cytotoxicity experiments.

• Restorative Dentistry and Endodontics
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• v.39 no.1
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• pp.39-44
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• 2014

Objectives: The purpose of this study was to evaluate in vitro cytotoxicity of the pozzolan cement and other root-end filling materials using human periodontal ligament cell. Materials and Methods: Endocem (Maruchi), white ProRoot MTA (Dentsply), white Angelus MTA (Angelus), and Super EBA (Bosworth Co.) were tested after set completely in an incubator at $37^{\circ}C$ for 7 days, Endocem was tested in two ways: 1) immediately after mixing (fresh specimens) and 2) after setting completely like other experimental materials. The methods for assessment included light microscopic examination, cell counting and WST-1 assay on human periodontal ligament cell. Results: In the results of microscopic examination and cell counting, Super EBA showed significantly lower viable cell than any other groups (p < 0.05). As the results of WST-1 assay, compared with untreated control group, there was no significant cell viability of the Endocem group. However, the fresh mixed Endocem group had significantly less cell viability. The cells exposed to ProRoot MTA and Angelus MTA showed the highest viability, whereas the cells exposed to Super EBA displayed the lowest viability (p < 0.05). Conclusions: The cytotoxicity of the pozzolan cement (Endocem) was comparable with ProRoot MTA and Angelus MTA. Considering the difficult manipulation and long setting time of ProRoot MTA and Angelus MTA, Endocem can be used as the alternative of retrofilling material.